The present invention belongs to the technical field of titanium alloy and the manufacturing method thereof and discloses a titanium alloy seamless tube and the manufacturing method thereof. The invention is mainly characterized by comprising a tube body. The tube body comprises the following components by weight: 90 parts of Ti, 3.5-4.5 parts of V, 5.5-6.5 parts of Al, 0.08-0.12 parts of Ru, 0.28 or fewer parts of Fe, 0.13 or fewer parts of O, 0.07 or fewer parts of C, 0.03 or fewer parts of N, and 0.015 or fewer parts of H. An anti-oxidation coating layer is repeatedly and uniformly coated on the blank material, and then is subjected to the operations of heating, skew-rolling, perforating, deforming, heating, tube-rolling and deforming at the temperature of 850-780 DEG C. Meanwhile, the multi-pass reducing and sizing deformation is conducted at the temperature of 850-780 DEG C, and then the straightening and shot-blasting treatment is finally conducted to obtain the finished products of titanium alloy tubes. The titanium alloy seamless tube and the manufacturing method are simple in process, more environmentally-friendly in manufacturing process, and wider in the dimension range of manufactured titanium alloy tubes. Manufactured products are good in microstructure and mechanical properties, and manufactured titanium alloy tubes have fewer surface defects. Manufactured titanium alloy seamless tubes are good in machining performance, such as low cost, easy forging and corrosion resistance, thus being widely applicable to complex corrosion environments.