The invention a printing and dyeing method, and especially relates to a production method for a polyester cotton oxygen bleaching and disperse dyestuff dyeing one bath process. The method comprises the following steps: selecting a sample cloth, carrying out pretreatment desizing, pre-setting a grey, singeing the grey, mercerizing, preparing a complex formulation assistant according to a formula, using the complex formulation assistant according to a ratio, designing a dyeing formula, carrying out jar dyeing on the cloth sample, carrying out hot setting, singeing the obtained color body, washing by water, drying, carrying out oiling setting, carrying out after-finishing calendering, carrying out after-finishing full decatizing, and examining the obtained finished product. The production method for the polyester cotton oxygen bleaching and disperse dyestuff dyeing one bath process has obvious energy saving and emission reduction effects, and the handle elasticity, the color fullness and various quality indexes of fabrics obtained in the invention can meet requirements of different clients.