The invention provides a plastic filling material used for
surgical treatment of a
bone defect and a preparation method thereof. The method comprises the steps of using an allogenous
cortical bone forpreparing bone grains in particle size of 0.1-1 mm; adopting a
chloroform / methyl
alcohol (1 to 1) solution for room-temperature soak
degreasing; in
purified water at 60+ / -1 DEG C, soaking the bone grains subjected to
degreasing for 10 h to conduct
virus inactivation; in
hydrochloric acid or
acetic acid, using an ultrasonic method for conducting
demineralization treatment, wherein the residual
calcium amount of the bone grains subjected to
demineralization treatment is lower than 8%; using
purified water for ultrasonic cleaning, and replacing the water hourly until a cleaning liquid pH is 5.8-7.5; conducting
freeze drying treatment, using two
layers of
bacteria-isolating plastic bags for packaging, and making the bone grains subjected to
irradiation sterilization for use; and in a sterileenvironment, preparing a
hyaluronic acid solution in a concentration of 2.5%-2.9%, and adding 450 mg of the bone grains into each milliliter of
hyaluronic acid solution for mixing. By means of the provided material and method, without changing bone induction activity of the
demineralized bone, the plastic mixture of the
demineralized bone matrix and
hyaluronic acid can be prepared, and can be directly used in clinic.