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Dentin collagen fiber demineralization material and preparation method and application thereof

A collagen fiber and dentin technology, applied in dental preparations, dentistry, dental prosthesis, etc., can solve the problems of dentin collagen degradation and high incidence of secondary caries

Pending Publication Date: 2019-10-15
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These shortcomings are the main factors responsible for the high incidence of secondary caries around the restoration and the degradation of dentin collagen

Method used

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  • Dentin collagen fiber demineralization material and preparation method and application thereof
  • Dentin collagen fiber demineralization material and preparation method and application thereof
  • Dentin collagen fiber demineralization material and preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0065] The material of this embodiment is to adopt the ethylene glycol chitosan (polymerization degree ≥ 400, MilliporeSigma, St.Louis, MO, USA) aqueous solution of suitable concentration to mix with EDTA aqueous solution, add 1-ethyl-3-( After the 3-dimethylaminopropyl)-carbodiimide cross-linking agent was continuously stirred at room temperature for 14 hours, unreacted residues were removed using appropriate dialysis conditions. Wherein the total amount of ethylene glycol chitosan is 10 g, EDTA 300 g, and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide cross-linking agent 15 g.

[0066] For example, a preferred dialysis condition is that the above reaction solution is dialyzed in a dialysis membrane with a molecular weight cut-off of 12-14 kDa and containing 0.05M NaOH to remove reactants with smaller molecular weights, and again with a molecular weight cut-off of 12-14 kDa and containing Dialyze against a dialysis membrane against double distilled water to remove uncrosslink...

Embodiment 2

[0069] This embodiment is different from embodiment 1 in that:

[0070] (1) ethylene glycol chitosan is dissolved in deionized water to form a chitosan solution with a concentration of 10 mg / mL;

[0071] (2) EDTA is dissolved in deionized water to form a concentration of EDTA aqueous solution of 300mg / mL;

[0072] (3) The above two solutions are mixed in equal volumes, and the pH of the mixed solution is adjusted to 6.0;

[0073] (4) Dissolve 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide crosslinking agent in the above mixture at a concentration of 15 mg / mL to make the carboxyl group on the EDTA molecular chain functional The group is covalently bonded to the amine functional group on the soluble chitosan skeleton, and is continuously stirred at room temperature for 14 hours;

[0074] (5) Place the above reaction solution in a dialysis membrane with a molecular weight cut-off of 12-14kDa and contain 0.05M NaOH for dialysis to remove reactants with a smaller molecular weight...

Embodiment 3

[0086] This embodiment differs from embodiment 2 in that:

[0087] (1) Carboxymethyl chitosan was dissolved in deionized water to form a chitosan solution with a concentration of 7 mg / mL.

[0088] (2) EDTA is dissolved in deionized water to form a concentration of 200mg / mL EDTA aqueous solution, the EDTA aqueous solution is mixed with carboxymethyl chitosan solution in equal volumes, and the pH of the mixed solution is adjusted to 6.0; the 1-ethyl-3 -(3-Dimethylaminopropyl)-carbodiimide cross-linking agent was dissolved in the above mixture at a concentration of 13 mg / mL, and kept stirring at room temperature for 12 hours.

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Abstract

The invention relates to a dentin collagen fiber demineralization material and a preparation method and an application thereof. In particular, the invention relates to a conjugate of water-soluble chitosan and an amino carboxylic acid metal chelating agent, and a use thereof for dental demineralization, preferably for dentin demineralization. The invention also discloses the dentin collagen fiberdemineralization material and the preparation method and the application thereof. The material is prepared from 0.5-1 part by mass of water-soluble chitosan, 10-30 parts by mass of EDTA and 0.8-1.5 parts by mass of a crosslinking agent. The invention also relates to a dental demineralization composition including the conjugate and the material. The conjugate, material and composition can be used for dentin demineralization treatment before dental adhesive repair. When being used as a dentin collagen demineralization agent, the conjugate, material and composition have excellent selective extrafibrous demineralization performance, good biocompatibility, effective bacteriostasis, inhibition of endogenous matrix proteinase activity and extremely low cytotoxicity.

Description

technical field [0001] The invention belongs to the field of dental bonding restoration materials, and in particular relates to a dentin collagen fiber demineralization material and a preparation method and application thereof. Background technique [0002] Teeth are highly mineralized organs in the human body. The normal dentin matrix is ​​composed of an orderly woven collagen fiber matrix, sequentially arranged inorganic phase apatite crystals and a small amount of non-collagenous proteins. [0003] According to the relative positional relationship between hydroxyapatite and collagen, the mineralization of dentin can be divided into two parts: intrafibril mineralization and extrafiber mineralization. Although 65% of the minerals in dentin are extrafibrillar, intrafibrillar mineralization is critical for maintaining fibrillar morphology and determines the mechanical properties of dentin at the nanoscale and prevents the incorporation of collagen in dentin. Denatured and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K6/097C08J3/24C08L5/08
CPCC08J3/24C08J2305/08A61K6/898A61K6/891A61K6/30C08L5/08A61K6/40A61K6/20A61K8/736
Inventor 牛丽娜陈吉华郑智明郭景梅余凡焦凯
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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