The invention relates to the field of research and development of biopesticide preparation, concretely, relates to a tetramycin complex and an application. the tetramycin complex comprises tetramycin and active substances; the active substances comprise one or more of the following substances: azoles, pyrimidinyl methanol, 2-amino-miazines, morpholines, aniline pyrimidines, pyrroles, benzamides, benzimidazoles, dicarboximides, methanamides, kresoxim-methyl, dithiocarbamates, N-halomethylthio-dicarboximides, copper compounds, nitrophenol derivatives, organophosphates, guanidines, trace elements, carbamates and pyrethroids. According to the invention, compared with the prior art, the tetramycin complex and the application are capable of reducing the danger of resistant fungal strains selectivity, prolonging the service life of active substances, enlarging prevention and treatment scope, increasing diseases prevention effect and delaying or overcoming resistance of pathogenic bacteria, the cost is saved, and the application frequency of pesticide can be reduced.