The invention provides a manufacturing method of antibacterial ceramic tiles, and belongs to the technical field of architectural ceramics. The manufacturing method of the antibacterial ceramic tiles is characterized by comprising the following steps: printing protective glaze after inkjet printing, wherein the protective glaze comprises the following raw materials in parts by weight: 11-13 parts of Qidao clay, 11-13 parts of calcium carbonate, 4.5-5.5 parts of barium carbonate, 4.5-5.5 parts of burnt bone stone, 28.5-32.5 parts of potassium feldspar, 14-16 parts of glaze frits, 1.7-2.3 parts of zirconium silicate, 15-18 parts of albite, 1.7-2.3 parts of zinc oxide and 7-9 parts of an antibacterial agent; and in the firing step, increasing the temperature of a kiln to 835-840 DEG C, insulating for 1.5-2.5 hours, then heating to the temperature of 1,200-1,210 DEG C, insulating for 3-4 hours, and then cooling. The protective glaze is suitable for the antibacterial agent to efficiently play effects for a long time, and by a ceramic tile firing mode for the glaze, the wear-resistant and long-acting antibacterial ceramic tile can be obtained.