2073 results about "Polyglutamic acid" patented technology

The present invention intends to provide a composition for hair treatment containing γ-polyglutamic acid or a salt thereof, a hair cosmetic for damaged hair containing such a composition, and their uses. The composition for hair treatment containing γ-polyglutamic acid or a salt thereof and the hair cosmetic for damaged hair of the present invention have excellent improvement effects on the strength and frictional force of hair, so that they can provide tension, elasticity, or the like to damage hair to prevent or alleviate split hair and broken hair as well as improvements in combing and touch. Furthermore, they also exert effects of moisture retention inherent to γ-polyglutamic acid or a salt thereof, preventing or improving effects on the generation of dandruff on the basis of such effects, preventing effects on the feeling of stickiness or creak, and various effectiveness including appropriate residual tendency to hair in a simultaneous manner, respectively.

Various biodegradable polyglutamate-amino acids comprising recurring units of the general formulae (I) and (II) are prepared. Such polymers are useful for variety of drug, biomolecule and imaging agent delivery applications.

In this invention, a release-delaying system is to be developed for LHRH antagonists, in particular for cetrorelix, which allows the active compound to be released in a controlled manner over several weeks by complexation with suitable biophilic carriers. The acidic polyamino acids polyglutamic acid and polyaspartic acid were selected for complexation with cetrorelix. The cetrorelix polyamino acid complexes are prepared from aqueous solutions by combination of the solutions and precipitation of the complexes, which are subsequently centrifuged off and dried over P2O5 in vacuo. If complexes having a defined composition are to be obtained, lyophilization proves to be a suitable method. The cetrorelix-carboxylic acid complexes were also prepared from the aqueous solutions. In the random liberation system, the acidic polyamino acids poly-Glu and poly-Asp showed good release-delaying properties as a function of the hydrophobicity and the molecular mass of the polyamino acid. In animal experiments, it was possible to confirm the activity of the cetrorelix-polyamino acid complexes as a depot system in principle. It is thus possible by complexation of cetrorelix with polyamino acids to achieve testosterone suppression in male rats over 600 hours. The release of active compound here can be controlled by the nature and the molecular mass of the polymers.

The invention provides a multifunctional aerogel material for blood component protection and a preparation method of the multifunctional aerogel material. Gellan gum, hyaluronic acid, Pullulan, Lubrajel CG andpoly(gamma-glutamic acid), derivatives or a mixture thereof are taken as a skeleton to act with a novellight-sensitive material, aerogel or a precursor for preparation of the ready-to-useaerogel material, and then multifunctional ready-to-useaerogel composite is prepared from the ready-to-useaerogel material as well as raw materials includingglucose, a novel solvent, a novel fixing agent, a buffering agent, a stabilizer, a novel preservative, a surfactant, a high-molecular compound, essential oil, an antioxidant and the likewith a three-spectral-line high-energy photocuring method by utilizing a high polymer material such as hyaluronic acid and the like as the skeleton. The material has the characteristics of being convenient to use, green, environment-friendly and the like, the whole blood component treated with the material keeps good cellular morphology, is stored for a long time, reducesbatch-to-batch difference and is an ideal additive for a whole blood controlling product and other products, and the material can also be applied to collection, storage and transfer of tissue cells as well as fields of cosmetics, food, drugs and the like.

The invention discloses a composite soil conditioner which belongs to the technical field of fertilizers, and mainly includes the following raw materials in parts by weight: 100-500 parts of humic acid with 45% of organic matter, 0.5-2 parts of ferrous sulfate, 8-100 parts of polyglutamic acid, 15-150 parts of polyaspartic acid, 1-50 parts of earthworm powder, 12-180 parts of shell powder, 10-50 parts of soybean cakes, 1-10 parts of amino-oligosaccharin, 10-30 parts of attapulgite powder and 10-20 parts of microorganism composite zymophyte. The composite soil conditioner provided by the invention can provide nutrition, adjust and balance the pH value of soil, adjust the soil microflora, improves the water retention, fertilizer maintenance and breathability of soil, degrade organic pollutants, releases phosphorus and potassium, has the antagonism function to heavy metals or can be used for precipitating heavy metals, reduces absorption of crops to heavy metals, and has the multiple conditioning functions of detoxifying the soil, building up fertility, improving the physical properties and micro-ecological environment of soil.

Methods for producing high molecular weight poly-gamma-glutamic acid (PGA). The PGA is produced by fermentation, and purified by use of tangential flow filtration, followed by diafiltration, as necessary, to yield a product of the desired purity. Product obtained may be of very high purity using all the prescribed purification steps. Product of this purity is suitable for in vivo medical applications. Other applications, such as food or agricultural, may utilize lower purity levels, and hence do not require all the purification steps specified.

The invention discloses a preparation method of biocompatible gamma-polyglutamic-acid hydrogel, which comprises the following steps of: by using polyglutamic acid as a main raw material and a biological-source material chitosan oligosaccharide as a cross-linking agent, carrying out preparation reaction in an aqueous solution, and activating a gamma-polyglutamic-acid carboxyl group by utilizing activating agents diamine carbide and N-hydroxysuccinimide ester to enable the polyglutamic acid and the free amino group of the chitosan oligosaccharide to form an amido bond to further form the hydrogel in a three-dimensional netted structure. After the prepared gel is swelled and balanced, drying is carried out by adopting a freeze-drying, vacuum-drying or microwave-drying mode. The biological-source material chitosan oligosaccharide is selected as the cross-linking agent, so that the biocompatibility of an obtained hydrogel system can be markedly improved; and a reaction system is an aqueous-solution system, reaction regulating and controlling properties are good, and the gel is stable. Because the hydrogel prepared by the method disclosed by the invention has good biocompatibility, the hydrogel can be widely applied to medicament carriers, auxiliary wound materials, medical and sanitary articles and the like.

The invention discloses a preparation method for a bacterial cellulose composite membrane and application of the bacterial cellulose composite membrane as a facial mask material. The preparation method comprises the following steps: (1) selecting a strain; (2) preparing a seed containing a polyglutamic acid (PGA) and a liquid culture medium; (3) culturing seed cells; (4) producing bacterial cellulose by static liquid fermentation; and (5) purifying a bacterial cellulose membrane. The bacterial cellulose composite membrane can be used as a facial mask carrier or is directly used as a wet facial mask. The preparation method improves the yield of the bacterial cellulose by adding the polyglutamic acid (PGA) into the culture medium, improves the water content and the mechanical strength of a wet membrane, and has simple process and low cost; and the experiments prove that the method can ensure that the dry weight of the bacterial cellulose is improved to between 14 and 16g / L from the prior dry weight of between 8 and 10g / L, and the water content is increased to 99 percent from 98 percent.

The invention provides a water soluble fertilizer containing high-concentration humic acid. The water soluble fertilizer comprises, by weight, 15-25 parts of a water soluble humic acid salt, 18-25 parts of urea, 9-15 parts of dipotassium hydrogen phosphate, 8-13 parts of potassium acetate, 0.5-2 parts of borax, 0.1-10 parts of polyglutamic acid, 1-5 parts of rhamnolipid, 5-10 parts of a hydroxymethyl cellulose aqueous solution and 30-40 parts of water. The water soluble fertilizer has humic acid content greater than or equal to 150g / L. The invention also provides a preparation method of the water soluble fertilizer containing high-concentration humic acid. The preparation method comprises the following steps of 1, carrying out heating stirring of the water soluble humic acid salt and water to obtain a humic acid salt solution, 2, adding dipotassium hydrogen phosphate, potassium acetate, borax, polyglutamic acid, rhamnolipid and urea into the humic acid salt solution, and carrying out heating stirring to obtain a mixed solution, and 3, carrying out emulsification of the hydroxymethyl cellulose aqueous solution and the mixed solution to obtain the water soluble fertilizer containing high-concentration humic acid. The water soluble fertilizer has obvious fertilizer efficiency, is environmentally friendly, stable and uniform and has a wide application range.

The present invention provides prodrugs comprising a polymer conjugated to a metal chelator via a disulfide bond. For example, D-penicillamine may be conjugated to a polymer (e.g., gelatin, chitosan, polyglutamic acid) via a linker, such as SPDP. Thus, the cellular delivery and pharmacokinetics of D-penicillamine can be substantially improved. Methods for the treatment of cancer using compositions of the present invention are also disclosed.

The invention discloses a preparation method of an embedding microbial agent for in-situ purification of a water body, which compounds a plurality of microbial strains suitable for the sewage treatment with enzyme preparation, and adds nutritive salt and growth factors needed by the microbial growth in an embedding carrier so as to be more suitable for the microbial growth, so that the defects of deficient nutrition of the water body and low sewage treatment efficiency are overcome. Active carbons with strong adsorption capacity and diatomite with greater specific gravity are added in the embedding carrier; polyglutamic acid is added in the embedding carrier; the polyglutamic acid reinforces the strength of embedding particles; meanwhile, glutamic acid obtained by degrading the polyglutamic acid can be used as nutritional ingredients for the strain growth, so that preparation can be preferably colonized in a river channel without easily losing. After the embedding microbial agent is used for treating sewage for three weeks, the ammonium-nitrogen removal rate reaches 85-90%, and the COD (Chemical Oxygen Demand) removal rate reaches 90-95%.

The invention provides a polyelectrolyte complex gel comprising a chitosan and a γ-polyglutamic acid (γ-PGA) having a molecular weight from about 1 kDa to about 400 kDa or the salt thereof, wherein the chitosan and the γ-PGA are swollen with an aqueous solution. Also provided is a soft tissue augmentation implant, comprising a polyelectrolyte complex gel of the invention as a carrier or a filler and an optional additive. The polyelectrolyte complex gel and the soft tissue augmentation implant containing the same has long degradation time and better supportability so as to provide good maintenance for soft tissue.

The invention discloses functional granular major-element water soluble fertilizer. The fertilizer comprises, by weight, 345-350 parts of urea, 210-220 parts of potassium nitrate, 240-250 parts of monopotassium phosphate, 20-30 parts of potassium sulphate, 110-115 parts of monoammonium phosphate, 11-11.6 parts of boric acid, 6-6.6 parts of EDTA chelated zinc, 7-7.7 parts of EDTA chelated iron, 3-3.9 parts of EDTA chelated manganese, 0.1-0.2 part of ammonium heptamolybdate and 5-10 parts of polyglutamic acid. Compared with the prior art, the functional granular major-element water soluble fertilizer is scientific in formula, complete in nutrition, balanced and stabilized in nutrient, capable of moisture retention and drought resistance and high in utilization rate and has the functions such as improving and restoring soil.

The invention discloses a method for preparing polyglutamic acid hydrogel. In the method, polyglutamic acid is used as a main raw material, glycol diglycidyl ether is used as a crosslinking agent and a pH value of the system is adjusted at regular intervals in the reaction process. The method comprises the following steps of: dissolving 15 to 30 percent of polyglutamic acid into distilled water, adding glycol diglycidyl ether in an amount which is 20 to 50 percent based on the molar amount of of polyglutamic acid monomers, uniformly stirring the mixed solution, adjusting the pH value of the system to 3 to 5, and performing a reaction at the temperature of between 40 and 80 DEG C for 4 to 10 hours, wherein the pH value of the system is adjusted to the initial value every 1 to 2 hours; and performing full swelling dialysis and drying of the prepared hydrogel. The method has the advantages that: the reaction is performed in aqueous solution, which contributes to environmental protection; and the preparation period is short, and the hydrogel prepared by the method is high in water absorption and high in swelling speed, and possesses bio-degradability. The hydrogel prepared by the method can be applied to medicament carriers, medical and hygienic products, water-retaining agents used in farming, forestry and gardening industries, thickening agents for cosmetics and foods, anti-condensation agents, flocculating agents, water treatment chemicals and the like.

The invention provides a copolymer with a structure of a formula (I), a preparation method thereof, glucose sensitive micelle containing the same, glucose sensitive medicine-carrying micelle containing the same and a preparation method of the glucose sensitive medicine-carrying micelle. The copolymer provided by the invention comprises a polyethylene glycol monomethyl ether hydrophilic chain segment and a polyglutamic acid hydrophobic chain segment, wherein the polyglutamic acid hydrophobic chain segment takes polyglutamic acid as a main chain and the branch chain contains phenylboric acid groups. In the copolymer, both polyethylene glycol monomethyl ether and the polyglutamic acid have good biocompatibility, the polyglutamic acid has good biodegradability, the carboxyl group has pH sensitivity, and the phenylboric acid group has glucose sensitivity. The copolymer provided by the invention has good biocompatibility, biodegradability, glucose sensitivity and pH sensitivity.

The invention provides a high-productive strain Bacillus subtillis CGMCC No.2108 of poly-Gamma -glutamic-acid; the invention also provides a culture method and an applicable culture medium of the strain. The poly-Gamma -glutamic-acid provided by the invention is has the simple requirement of strain nutrient, stable passage, maximum output of 34.1g per liter, simple and practical culture method, wide culture medium raw material source and low cost, thus effectively reducing the production cost and being suitable for large-scale industrial production.

The present invention includes PG polymer complexes with paramagnetic materials, such as Gd, Mn and iron oxide. The complexes may also include chelating agents which may be covalently attached to the PG polymer backbone through linkers. PEG may also be attached to the PG polymer backbone. The complexes may include targeting molecules. The complexes are useful as MRI contrast agents, particularly as blood pool agents.

Production of gama-polyglutamic acid by fermentation is carried out by: culturing bacillus subtills zju-7 strain, i.e., CGMCC No. 1250, in medium with carbon and nitrogen sources, glutamic acid, MgSO4, NaCl and K2HPO4 to obtain ferment liquid, and making it to pass organic solvent settlement and dialysis. The process is easy, yields highly, and costs low with quality products.

The invention discloses an organic / inorganic slow-release fertilizer for flowers and a preparation method of the slow-release fertilizer. The organic / inorganic slow-release fertilizer is prepared from the following raw materials in parts by weight: 45 to 55 parts of horse manure, 20 to 30 parts of wheat bran, 15 to 25 parts of brewer's grains, 12 to 18 parts of flour beetle frass sand, 10 to 15 parts of residual tea, 5 to 10 parts of edible fungi waste, 18 to 26 parts of sorgo straw, 10 to 15 parts of mung bean shells, 5 to 10 parts of sugar beet meal, 1 to 3 parts of spawn powder, 16 to 22 parts of yellow phosphorus furnace slag, 20 to 30 parts of obsidian, 17 to 24 parts of hectorite, 10 to 15 of parts zeolite 4A, 32 to 44 parts of ammonium nitrate, 22 to 28 parts of ammonium hydrogen phosphate ammonium dihydrogen phosphate, 8 to 14 parts of potassium carbonate, 2 to 3 parts of diaminoferric xanthohumate, 3 to 6 parts of sugar calcium, 4 to 8 parts of sodium metasilicate, 15 to 20 parts of polyvinyl alcohol, 5 to 10 parts of carboxymethyl cellulose, 9 to 16 parts of hydroxypropyl xanthan gum, 2 to 4 parts of algal polysaccharides, and 3 to 5 parts of polyglutamic acid. The fertilizer provided by the invention is high in nutrient amount and high and long in fertilizer efficiency, can improve soil, enables soil to form granular structures, and harmonizes water and gas in soil to enable flowers to grow up well and quickly, thereby improving the quality and the yield of flowers and lowering the fertilization cost; important economic benefits are brought.

The invention discloses a strain for synthesizing gamma-polyglutamic acid. The strain is screened from soil around a chemical fertilizer plant and is classified to be named as Bacillus Subtilis NS-9, the collection number of the strain in the China Center for Type Culture Collection (CCTCC NO.) is M2014142, and the collection date of the strain is April 23, 2014. The invention also discloses a method for efficiently preparing gamma-polyglutamic acid by using the strain for synthesizing gamma-polyglutamic acid. Compared with an existing gamma-polyglutamic acid production technique, the adopted bacillus subtilis microbial strain disclosed by the invention can be used for efficiently producing gamma-polyglutamic acid by virtue of fermentation; by optimizing the culture conditions of the bacillus subtilis strain, particularly replenishing a carbon source, inorganic salts and energy substances in a fermentation process, the content of gamma-polyglutamic acid in a fermentation solution can reach 100-200g / L, so that the method for preparing gamma-polyglutamic acid, disclosed by the invention, is high in efficiency and low in cost.

The invention provides a microbial flocculating agent prepared through using two bacilli. The bacilli comprise Bacillus megaterium and Bacillus subtilis, wherein the Bacillus subtilis can generate a high-molecular biobased material gamma-polyglutamic acid, and two broths of the two bacilli are mixed and are added with a suspension substance to flocculate the suspension substance and generate a solid flocculating constituent. The microbial flocculating agent can substitute chemical flocculating agents, can be safely used in various industrial sewage processing industries, the food industry and the medicine industry, and has a popularization prospect.

The invention discloses a novel high-strength hydrogel which is prepared by the following steps: synthesizing a first network hydrogel from a polyelectrolyte (such as hyaluronate, polyglutamic acid, chondroitin sulfate or polylysine); and after soaking the hydrogel in a double-bond functional polyethyleneglycol water solution for some time, taking out, and crosslinking to obtain the high-strength double-network hydrogel. The synthesized high-strength hydrogel has favorable biocompatibility, can be used as a tissue engineering / repair scaffold material or the like, and has wide applicability in the field of biomedical materials.

The invention discloses a seed coating, which is characterized by being formed by mixing 65-80% of gamma-polyglutamic acid, 15-30% of dispersant, 0.5-2% of bonder, 0.05-1% of surfactant, 0.05-2% of biocide and 0.1-0.5% of vigilant dye by weight percent. According to the seed coating taking polyglutamic acid as a primary component, the seed growth microenvironment can be improved, the germination of seeds and the growth and development of seedlings can be promoted, the quality of rice seedlings can be improved, the pesticide effect of pesticides can be improved, the pesticides diffused in the environment and the contact amount of non targets can be reduced, no residues are generated on the leaf surfaces, the disease prevention and pest control effects of the seed coating are improved, and the requirements of green ecological agriculture development are met.

The invention discloses a hydrogel based on a gamma-polyglutamic acid and epsilon-polylysine cross-linked polymer. The hydrogel is prepared from crosslinking gamma-polyglutamic acid and epsilon-polylysine, and has a polymer with the following structural units, wherein m is a natural number ranging from 15 to 45, n is a natural number ranging from 3900-17000 and x is a natural number ranging from 5 to 40. The invention further discloses a preparation method of the hydrogel and an application of the hydrogel in preparing medical wound dressing. The hydrogel prepared by the method disclosed by the invention has good biocompatibility and can be widely applied to medical sanitary articles such as wound dressing.

The invention discloses a strain of gamma-polyglutamic acid producing bacterium. The gamma-polyglutamic acid producing bacterium is classified and named as Bacillussubtilis PGS-1 and is collected in China General Microbiological Culture Collection Center (CGMCC), wherein the collection number of a bacterial strain is CGMCC No.4034; and the collection date is July 23rd, 2010. The invention also discloses a method for preparing gamma-polyglutamic acid and salts thereof by using the gamma-polyglutamic acid producing bacterium. Compared with the prior art, the gamma-polyglutamic acid synthesized by the gamma-polyglutamic acid producing bacterium of the invention has the advantages of lower molecular weight (300 to 400 KDa), narrower molecular weight distribution, suitability for the field of application with a low molecular weight requirement and extremely wide application prospects.

The invention discloses a biological type organic-inorganic compound fertilizer for improvement in saline land and a preparation method thereof. The preparation method includes the steps that attapulgite, potassium humate and zeolite are ground into mixture powder, the mixture powder is mixed with polyglutamic acid and wood vinegar, water is added for constant-temperature stirring, dissolving liquor is obtained and then evenly mixed with a mixture of urea, monoammonium phosphate, potassium sulfate, ammonium sulfate and trace element fertilizer which are mixed evenly and heated to be molten, the mixture is subjected to granulating, drying and cooling, a microbial agent is sprayed on the surface of the cooled granulated mixture, then the mixture is evenly mixed with citric acid, calcium superphosphate, polyacrylamide and livestock and poultry fermented organic fertilizer, and the biological type organic-inorganic compound fertilizer is obtained. The prepared biological type organic-inorganic compound fertilizer can effectively reduce salt and alkaline in saline alkali soil and integrates the advantages of organic fertilizer, inorganic fertilizer and slow release fertilizer, the utilization rate of the fertilizer can be increased, and the improvement effect on soil of microbial floras is effectively utilized; the fertilizer has the advantages of being long in duration of fertilizer efficiency, good in fertilizer efficiency, environmentally friendly and free of pollution.

The present invention discloses a high-yield gamma-polyglutamic acid production gene engineering bacterial and a fermentation production method thereof. The gene engineering bacterial is named Bacillus subtilis FRD518, wherein the preservation number is CGMCC NO.6772, and the Vitreoscilla hemoglobin gene (vgb) is recombined and integrated on the chromosome of the gene engineering bacterial, such that the Vitreoscilla hemoglobin VHb can be successfully and highly expressed so as to significantly improve the oxygen utilization rate of the recombinant Bacillus subtilis under a low dissolved oxygen condition. According to the present invention, during a fermentation process, a carbon source, sodium glutamate, a yeast extract and other components are added in a flow manner, such that the gene engineering bacterial can efficiently produce the gamma-polyglutamic acid in a high yield manner, wherein the yield achieves more than 65 g / L, and is increased by 147% compared with the yield of the single batch culture of the original wild strain; and with the gene engineering bacterial, the problems of low yield, more by-products, long period, high energy consumption and the like of fermentation of the gamma-polyglutamic acid gene engineering bacterial under high viscosity and dissolved oxygen limiting conditions are solved, and the gamma-polyglutamic acid gene engineering bacterial can be applied for large-scale industrial production of gamma-polyglutamate acid.

The preparation method includes the following steps: modifying hepatic target compound onto degradable polymer (chitosan, polylysine, glucosan, agar, polyglutamic acid-benzyl ester, polyalanine) with biological compatibility, and adopting ion exchange or ultrasonic emulsification process to obtain the invented nano hepatic target bio-degradable medicine carrier material. The hepatic target nano particle solution has good target performance for liver, the medicine enriched rate in the liver can be up to 75%, and its slowly-released administration can be up to above 15 days.

The invention discloses an anti-aging composition containing an epidermal growth factor and a preparation method thereof. Human oligopeptide-1 is used as a main active compound and is compounded with polyglutamic acid, a lactobacillus fermentation product, ectoin and other functional active compounds, under a synergic effect of the functional active compounds, the human oligopeptide-1 can fully play biological activity, and the anti-aging composition better reaches the anti-aging, repairing and high-efficiency moisturizing functions, restores skin bright, smooth and shiny, improves skin conditions of being dry, rough, dull, aged, large in pores and the like, and makes skin healthy and smooth. Tests prove that the composition has outstanding moisturizing and skin aging delaying efficacies compared with other skin care products.