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Gamma-polyglutamic acid producing bacterium and method for preparing gamma-polyglutamic acid and salts thereof by using gamma-polyglutamic acid producing bacterium

A technology of polyglutamic acid and glutamate, which is applied in the field of microorganisms and fermentation, and can solve problems such as high cost and cumbersome process

Inactive Publication Date: 2011-01-19
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-molecular-weight γ-PGA (1000KDa) is useful as a thickener and the preparation of superabsorbent resins. There are many reports on this type of γ-PGA synthetic bacteria; lower molecular weight γ-PGA (100KDa) is used for the control and slowing of drugs. release carrier is more favorable, but it has not been able to screen out a strain that can efficiently synthesize γ-PGA and is suitable for industrial production. Its preparation can only be made by physical or chemical degradation, which is cumbersome and costly; in addition, the molecular weight is narrow Distributed γ-PGA is more conducive to its fine application

Method used

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  • Gamma-polyglutamic acid producing bacterium and method for preparing gamma-polyglutamic acid and salts thereof by using gamma-polyglutamic acid producing bacterium
  • Gamma-polyglutamic acid producing bacterium and method for preparing gamma-polyglutamic acid and salts thereof by using gamma-polyglutamic acid producing bacterium
  • Gamma-polyglutamic acid producing bacterium and method for preparing gamma-polyglutamic acid and salts thereof by using gamma-polyglutamic acid producing bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Slant medium components: peptone 8g / L, yeast extract 10g / L, sodium glutamate 10g / L, agar 20g / L, K 2 HPO 4 ·3H 2 O 2g / L, NaCl 10g / L, CaCl 2 1g / L, MgSO 4 1g / L, pH 7.0.

[0050] Seed medium preparation: glucose 20g / L, yeast extract 15g / L, sodium glutamate 10g / L, NaCl 10g / L, K 2 HPO 4 ·3H 2 O 2g / L, CaCl 2 1g / L, MgSO 4 1g / L, pH 7.0, sterilized at 121°C for 15 minutes.

[0051] Preparation of fermentation medium: glucose 30g / L, yeast extract 25g / L, sodium glutamate 20g / L, NaCl 10g / L, K 2 HPO 4 ·3H 2 O 20g / L, CaCl 2 1g / L, MgSO 4 1g / L, pH 7.0, sterilized at 121°C for 15 minutes.

[0052] The cryopreserved CGMCC No.4034 strain was inoculated on a slant medium for activation, and cultured at 37°C for 8 hours. The CGMCC No.4034 activated on the slant was inoculated into the seed medium, the shaker rotated at 200r / min, and cultured at 37°C for 10 hours.

[0053] Put the seed liquid obtained in the previous step into the shake flask fermentation medium, the inoc...

Embodiment 2

[0056] Slant-plane activation of strains: the same as in Example 1.

[0057] Seed medium preparation: glucose 20g / L, beef extract 15g / L, sodium glutamate 10g / L, NaCl 10g / L, K 2 HPO 4 ·3H 2 O 2g / L, CaCl 2 1g / L, MgSO 4 1g / L, pH 7.0, sterilized at 121°C for 15 minutes.

[0058] Fermentation medium preparation: glucose 30g / L, beef extract 30g / L, sodium glutamate 50g / L, NaCl 10g / L, K 2 HPO 4 ·3H 2 O 20g / L, CaCl 2 1g / L, MgSO 4 1g / L, pH 7.0, sterilized at 121°C for 15 minutes.

[0059] The cryopreserved CGMCC No.4034 strain was inoculated on a slant medium for activation, and cultured at 37°C for 8 hours. Inoculate the seed medium with CGMCC No.4034 activated on the slant, and culture at 35° C. for 12 hours at a shaker speed of 200 r / min.

[0060] Put the seed liquid obtained in the previous step into the fermentation medium of the shake flask, the inoculum size is 10% (v / v), the liquid volume of the shake flask is 200mL / 500mL, the shaker speed is 150r / min, and cultiva...

Embodiment 3

[0063] Slant-plane activation of strains: the same as in Example 1.

[0064] Seed medium preparation: glucose 20g / L, peptone 15g / L, glutamic acid 10g / L, NaCl 10g / L, K 2 HPO 4 ·3H 2 O 2g / L, CaCl 2 1g / L, MgSO 4 1g / L, pH 7.0, sterilized at 121°C for 15 minutes.

[0065] Fermentation medium preparation: glucose 30g / L, peptone 30g / L, glutamic acid 70g / L, NaCl 10g / L, K 2 HPO 4 ·3H 2 O 20g / L, CaCl 2 1g / L, MgSO 4 1g / L, pH 7.0, sterilized at 121°C for 15 minutes.

[0066] The cryopreserved CGMCC No.4034 strain was inoculated on a slant medium for activation, and cultured at 37°C for 8 hours. The CGMCC No.4034 activated on the slant was inoculated into the seed medium, the shaker rotated at 300 r / min, and cultured at 30° C. for 15 hours.

[0067] Put the seed solution obtained in the previous step into the shake flask fermentation medium, the inoculum size is 10% (v / v), the shake flask liquid volume is 50mL / 500mL, the shaker speed is 400r / min, and cultivated at 30°C for 7...

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Abstract

The invention discloses a strain of gamma-polyglutamic acid producing bacterium. The gamma-polyglutamic acid producing bacterium is classified and named as Bacillussubtilis PGS-1 and is collected in China General Microbiological Culture Collection Center (CGMCC), wherein the collection number of a bacterial strain is CGMCC No.4034; and the collection date is July 23rd, 2010. The invention also discloses a method for preparing gamma-polyglutamic acid and salts thereof by using the gamma-polyglutamic acid producing bacterium. Compared with the prior art, the gamma-polyglutamic acid synthesized by the gamma-polyglutamic acid producing bacterium of the invention has the advantages of lower molecular weight (300 to 400 KDa), narrower molecular weight distribution, suitability for the field of application with a low molecular weight requirement and extremely wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and fermentation, and in particular relates to a synthetic bacterial strain of gamma-polyglutamic acid and a method for preparing gamma-polyglutamic acid and salts thereof with low molecular weight and narrow molecular weight distribution. Background technique [0002] γ-polyglutamic acid (γ-polyglutamic acid, hereinafter referred to as γ-PGA) is a type of homopolyamino acid formed by linking D-glutamic acid and L-glutamic acid monomers through γ-amide bonds. Fermentation synthesis. Because γ-PGA has excellent physical, chemical and biological properties such as film-forming, fibroblast, plasticity, adhesion, moisture retention, biocompatibility and degradability, it is widely used in industry, agriculture, food and medicine, etc. The field has great application potential. [0003] Most of the γ-PGA producing bacteria reported in the literature are Bacillus species (such as Bacillus anthr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/00C12R1/125
Inventor 姚俊曹新魏钦俊鲁雅洁孙荣斌阮文辉
Owner NANJING MEDICAL UNIV
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