104 results about "Bacillus species" patented technology

Compositions including a non-pathogenic lactic acid-producing bacteria, such as a Bacillus species, spores or an extracellular product of B. coagulans, formulated for oral administration to the intestinal tract for inhibiting bacterial gastrointestinal infections are described. Methods and systems using the compositions for treating gastrointestinal infections, particularly sudden infant death syndrome (SIDS) are also disclosed.

The present invention discloses compositions derived from an isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain, suitable for topical application to the skin or mucosal membranes of a mammal, which are utilized to inhibit the growth of bacterium, yeast, fungi, virus, and combinations thereof. The present invention also discloses methods of treatment and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, by topical application of therapeutic compositions which are comprised, in part, of isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain. In addition, the present invention also discloses compositions, methods of treatment, and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, comprising an extracellular product of Pseudomonas lindbergii comprising a supernatant or filtrate of a culture of said Pseudomonas lindbergii strain.

The present invention relates to thermostable proteinases from thermophilic Bacillus species and their uses in the preparation of nucleic acid samples. The enzymes of the invention are stable and active at 65-80° C., but are readily autolysed or denatured above 90° C.

The present invention relates to thermostable proteinases from thermophilic Bacillus species and their uses in the preparation of nucleic acid samples. The enzymes of the invention are stable and active at 65-80 ° C., but are readily autolysed or denatured above 90° C.

Disclosed is a method for detecting the species of a bacterium in a simple manner. Specifically disclosed is a method for detecting the species of a bacterium, which can detect at least one bacterial species selected from Staphylococcus species, Streptococcus species, Klebsiella species, Escherichia species, Mycobacterium species, Legionella species, Vibrio species, Bacillus species, Neisseria species, Campylobacter species, Chlamydia species, Chlamydophila species, Mycoplasma species, Listeria species, Salmonella species and Yersinia species, and which comprises the steps of: a) contacting a sample suspected to contain a nucleic acid derived from the bacterium species to be detected with a nucleic acid molecule capable of hybridizing with at least a part of a DnaJ gene derived from the bacterial species; and b) detecting the presence or absence of the hybridization between the nucleic acid molecule and the nucleic acid contained in the sample.

The present invention discloses compositions derived from an isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain, suitable for topical application to the skin or mucosal membranes of a mammal, which are utilized to inhibit the growth of bacterium, yeast, fungi, virus, and combinations thereof. The present invention also discloses methods of treatment and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, by topical application of therapeutic compositions which are comprised, in part, of isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain. In addition, the present invention also discloses compositions, methods of treatment, and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, comprising an extracellular product of Pseudomonas lindbergii comprising a supernatant or filtrate of a culture of said Pseudomonas lindbergii strain.

The invention relates to a wastewater treatment device and a wastewater treatment method using the same. The wastewater treatment device comprises a plurality of biological reaction tanks, wherein each biological reaction tank comprises wastewater and activated sludge which are introduced to the biological reaction tank; a reticular rotating-type Bacillus contact device is arranged in at least one biological reaction tank; the contact device comprises a plurality of reticular rotating-type Bacillus contacts arranged around a rotation axis side by side at a specified interval; microbial films are attached onto the reticular rotating-type Bacillus contacts; both the microbial films and the activated sludge are microbes with mixed bacteria of Bacillus as dominants; and the lower regions of the contacts are partially soaked in the activated sludge. By using the wastewater treatment device provided by the invention, stable wastewater treatment can be achieved, the installation space of the treatment equipment can be substantially reduced and the cost is lowered.

Genetically modified microorganisms having the ability to produce D(−)-lactic acid at temperatures between 30° C. and 55° C. are provided. In various embodiments, the microorganisms may have the chromosomal lactate dehydrogenase (ldh) gene and / or the chromosomal acetolactate synthase (alsS) gene inactivated. Exemplary microorganisms for use in the disclosed methods are Bacillus spp., such as Bacillus coagulans.

The invention discloses a production method of a probiotic soil conditioner. The production method comprises the steps of: (1) respectively inoculating huge bacillus, bacillus subtilis and bacillus licheniformis into a seed cultivation medium to cultivate and obtaining respective seed liquid; (2) adding the cultivation medium for production into a fermentation tank to sterilize and cool; and (3) inoculating the bacillus seed liquid into the fermentation tank to cultivate under an oxygen-leading agitating condition, inoculating the bacillus subtilis seed liquid and bacillus licheniformis seed liquid to cultivate, and obtaining the probiotic soil conditioner. The invention also discloses the probiotic soil conditioner obtained by the method. The method disclosed by the invention is scientific and reasonable, simple and feasible in production process and strong in industrialization implementation, and has an important significance to the development of agricultural production.

The invention discloses a preparation method of a feather fermentation protein feed. The preparation method is characterized by comprising the following steps of adding each kilo of feather into 20 to 30l of inorganic salt culture medium for mixing, sterilizing at the temperature of 115 to 121DEG C for 15 to 20 minutes, and cooling to room temperature; inoculating a bacillus licheniformis seed solution into the inorganic salt culture medium in which the feather is added according to the volume ratio of 3 to 5 percent; introducing 0.15 to 0.18 cubic meter of sterile air into each cubic meter of solution in each minute for ventilating and fermenting at the fermentation temperature of 30 to 37DEG C until the feather is completely dissolved; performing filtering and impurity removal on fermentation liquor, then adding maize flour with the mass percent of 6 to 7 percent and bran with the mass percent of 4 to 5 percent into filtrate, uniformly mixing, drying and granulating to obtain the feather fermentation protein feed. According to the method disclosed by the invention, the content of soluble protein in the fermentation liquor reaches 31.4 to 34.3g / L; for the fermentation protein feed prepared from the maize flour, the bran and flour serving as auxiliary materials, the mass percent of crude protein reaches 28.6 to 29.5 percent, and the protein digestibility in vitro reaches 89.5 to 92.4 percent. The protein feed prepared by the method disclosed by the invention has natural maize and wheat fragrance, is good in palatability, and is conductive to digesting and promoting the growth.

The invention relates to a soil improving method for improvement of subway shield soil and dredged soil and greening of landfill yards. The method is characterized by comprising the following steps of (1), adding an improver and a microbial inoculant into the soil to improve the soil, wherein the improver contains the following main components of turf, grain and perlite, the inoculant of the microbial inoculant is bacillus megatherium, and a carrier is peat dust; and (2), improving a nitrogen-fixing plant of Lucerne, utilizing the Lucerne which is a legume and has higher biological nitrogen-fixing capability, and sowing the Lucerne on the ground with flattened soil, wherein the sowing amount is 2 to 3g / m<2>, the sowing period is May to August, and plants are ploughed into the soil after autumn. The method is characterized in that the microbial inoculant is inoculated onto a liquid culture medium, and is subjected to shaking culture for 2 to 3 days, a 200ml parent inoculant solution is obtained from each bottle of freeze-drying inoculant (bacillus megatherium), the parent inoculant solution is inoculated into the sterile peat dust with a humidity of 50% to 60%, and 0.5kg peat dust is inoculated into every 10ml of inoculant solution. The method has the advantage that the soil structure and the quality difference of the subway shield soil and the dredged soil are improved, so the growth and the development of the greening plants planted in a sealed area can be met.

The invention discloses a method for preparing a biological compound demulsifying agent, which relates to a method for preparing a demulsifying agent. The method solves the problems that the harms to the environment caused by a chemical demulsifying agent still exist although the conventional biological demulsifying agent is compounded with the chemical demulsifying agent or auxiliary agents with other functions, and the biological demulsifying agent has low demulsifying efficiency, poor stability and high cost. The method comprises the following steps: firstly, preparing a bacillus mojavensis seed solution; secondly, preparing a bacillus subtillis seed solution; and finally, mixing the bacillus mojavensis seed solution and the bacillus subtillis seed solution, and inoculating the mixture into a culture medium for culture to obtain the biological compound demulsifying agent. The biological compound demulsifying agent has an oil expulsion rate up to 100 percent, reduces the addition and the cost, has high stability which is shown by good demulsifying effect on emulsions under the condition of the pH value of 3 to 9, and the change of the oil expulsion rate of not more than 10 percent when the temperature of the emulsions changes between 20 and 120 DEG C.

The present invention refers to a process for increasing the production of biomass and metabolites of microorganisms of Bacillus sp. species. Obtained metabolites are lipopeptide compounds of the fengycin, surfactin, and iturin families, which exhibit antimicrobial activity. The invention further includes biocidal compositions comprising Bacillus subtilis EA-CB0015, Bacillus amyloliquefaciens EA-CB0959, and / or metabolites thereof, either alone or together with other biocidal agents, and the use of these compositions for the treatment of diseases caused by various phytopathogenic agents, including Mycosphaerella fijiensis, in a variety of crops.

The invention provides a preparation method of a sea cucumber bait. The preparation method solves the technical problems of instable quality, unreasonable nutritional composition, immature production process, less microbe content, not ideal effect, and the like of a raw material. The preparation method comprises the following steps of: inoculating lactic acid bacteria strains, microzyme strains, photosynthetic bacteria strains and bacillus stains to aquatic feed yeasts, float sludge and zostera marina powder in purified seawater, wherein the balance is the purified seawater, and the content is in terms of weight percent; carrying out liquid-state fermentation on a mixture at room temperature; and adding vitamins, immune polysaccharide, an enzyme preparation, amino acid peptides and radix astragali polysaccharide to a product to prepare the sea cucumber bait. The preparation method provided by the invention can be used for preparing the sea cucumber bait.

The invention provides a microecological microbial agent for resisting banana vascular wilt and a preparation method thereof. A fermentation medium for the fermentation liquor of the microecological microbial agent for resisting the banana vascular wilt is prepared from the following components in percentage by weight: 0.1 to 10 percent of corn flour, starch, cane sugar or molasses, 1 to 10 percent of bean pulp, soybean meal, bran or beef extract and 1,000ml of water under the condition of the pH value of 6 to 9. The method comprises the following steps of: preparing bacillus subtilis seed liquor; inoculating the prepared bacillus subtilis seed liquor to the fermentation medium for culturing; and collecting the bacillus subtilis fermentation liquor after the fermentation is finished. In the microbial agent and the preparation method, the raw materials of the fermentation medium are economic and cheap; and the fermentation medium has a proper concentration, inoculation quantity and bottling quantity, so that the fermented product has higher activity for resisting the banana vascular wilt. The formula of the fermentation medium and the fermentation condition can make a fermentation product have high antibacterial activity and a low cost. The adopted detection method is fast and convenient and a straightforward detection result can be obtained by the detection method.

The invention discloses a preparation method of a compound microorganism deodorant. The preparation method respectively comprises the following steps: preparing environment-friendly enzyme, preparinga photosynthetic bacteria seed solution, preparing a saccharomycetes seed solution, preparing a lactobacillus seed solution, preparing an actinomycetes seed solution, preparing a bacillus seed solution and preparing an acetic bacteria seed solution; then, mixing the photosynthetic bacteria seed solution, the saccharomycetes seed solution, the lactobacillus seed solution, the actinomycetes seed solution, the bacillus seed solution and the acetic bacteria seed solution according to a certain proportion, and putting the mixed solution in a brown sugar culture medium for culturing, thus obtaininga mixed microorganism fermentation solution; finally, uniformly mixing the environment-friendly enzyme with the mixed microorganism fermentation solution according to a volume ratio which is (0.5 to 1):(1 to 1.5), thus obtaining the compound microorganism deodorant. All microorganisms in the compound microorganism deodorant are in mutual promotion and synergy effects, bio-organic matters generating an odor smell can be effectively decomposed, a deodorizing effect is good, and an application range is wide.

Four yeasts (NRRL Y-30213, NRRL Y-30214, NRRL Y-30215, and NRRL Y-30216) and 1 bacterium (NRRL B-30212) have been identified as being superior antagonists capable of suppressing Fusarium head blight (head scab) in cereals, particularly in wheat and barley. Fusarium head blight is primarily caused by the fungus Gibberella zeae (anamorph=Fusarium graminearum).

A preparation method for bouquet natto belongs to the technical scheme of food microorganism and fermentation engineering. The method has following steps: beans with high content of vegetable proteins is selected, soaked in water, sterilized at high temperature and pressure, bacterized with bacillus subtilis seed fermentation broth, fermentated, after-matured. The invention is characterized in that the after-matured material is then added with barmy carbohydrate, bacterized with Saccharomyces cerevisiae, fermentated and after-matured to obtain the finished product. The invention processes secondary fermentation based on the prior preparation method of natto, bacterizes Saccharomyces cerevisiae and adds barmy carbohydrate which provides plenty of nutrients for the growing of Saccharomyces cerevisiae, thus providing good condition for improving the flavor of original natto, completely eliminating the special odious smell and the beany flavor of the original natto, maintaining the original ropiness and completely utilizing the original nutrients. The prepared natto has pure flavor and high nutritional components.

A method is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity derived from Bacillus sp. to produce peroxycarboxylic acids.

The invention discloses a method for preparing a phosphorus-containing organic compound fertilizer. The method comprises working procedures of pretreating grain stillage, preparing the phosphorus-containing organic compound fertilizer and granulating and packaging and specifically comprises the following steps of: (1) pretreating the grain stillage, namely uniformly mixing the grain stillage and water, inoculating an aspergillus fumigatus seed nutrient solution, and fermenting by raw materials; (2) preparing the phosphorus-containing organic compound fertilizer, namely inoculating more than one of an aspergillus niger seed nutrient solution, a bacillus megaterium seed nutrient solution, a lactobacillus seed nutrient solution and a white rot fungi seed nutrient solution into 25 to 65 parts of pretreated grain stillage, 35 to 60 parts of phosphate tailing powder and 5 to 20 parts of water, uniformly mixing, and fermenting by the raw materials; and (3) granulating the fermented organic compound fertilizer and packaging. According to the method, main wastes of a wine industry and main wastes of a phosphorite industry are used comprehensively and recycled; and a prepared product can be used for agricultural production, so that the yield of crops is increased, and the fertilizing soil is improved.

The invention provides methods and compositions for rapid, sensitive, and highly specific nucleic acid-based detection of B. anthracis in a sample. In general, the methods involve detecting within the sspE gene of B. anthracis the presence of a fragment of six unique contiguous nucleotides that is not present in the sspE genes of other Bacillus bacteria or other non-Bacillus bacteria. In many embodiments, the methods involve amplifying a nucleic acid comprising at least a fragment of an sspE gene from a sample, and detecting the presence of the six unique contiguous nucleotide fragments in the nucleic acid. The invention also provides primers and kits for detection of B. anthracis in a sample. The subject invention finds use in a variety of different applications, including research, medical, diagnostic and military applications.

The invention belongs to the technical field of industrial wastewater treatment, and particularly relates to a method for immobilized culture of salt-tolerant bacillus strains. The method mainly comprises the following steps that salt-tolerant bacillus strains are inoculated in a salt-tolerant culture medium for activated growth; the salt-tolerant bacillus strains obtained after activated growth are inoculated on an activated plate culture medium for culture; the strains obtained after being cultured on the activated plate culture medium are inoculated to a salt-tolerant culture medium containing magnetic nanometer materials for shake culture; standing and separation of a culture medium system are performed in a magnetic field, and salt-tolerant bacillus strain precipitations loaded with magnetic nanometer particles are centrifuged and collected. The salt-tolerant bacillus strains loaded with magnetic nanometer particles can be applied to industrial wastewater treatment and have the advantages of being easily separated from wastewater, not likely to be lost, easy to use, low in cost and high in degradation efficiency.

The invention belongs to microbial curing, and in particular relates to a method for preparing calcium carbonate by directly extracting microbial strains from soil. The method comprises the followingsteps: 1, extracting and separating out bacillus species from the soil with high organic matter content; 2, breeding and cultivating the bacillus species separated out in step 1; 3, mixing the bacillus liquid obtained in the step 2 with gelling liquid according to a equal volume proportion, putting the mixture into an air bath incubator at a constant temperature of 30 DEG C for curing for a periodof time, and then filtering the mixed liquid to obtain microbial induced calcium carbonate precipitate. The method can be used for preparing the calcium carbonate by directly extracting the microbialstrains from the soil; urea and nickel sulfate are selected as a solid selection medium for separating the bacillus species, so that the separation efficiency is high; the separated bacillus speciesare mixed with the gelling liquid, and the mixture is cured under the proper conditions, so that microbial induced calcium carbonate precipitate is obtained. The method is economical and environmentally-friendly, easy to operate and high in calcium carbonate yield.

Genes have been identified in the Bacillus genome that are responsive to various metabolic conditions and growth cycle changes. The new responsiveness of these genes allows for their use in regulated gene expression in Bacillus sp. and for the monitoring of bioreactor health.

The invention discloses a medium for improving the antibacterial activity of biocontrol bacillus to plant pathogenic fungi, comprising the following components: potato, sucrose, maize paste, sodium L-glutamate, phosphate, MgSO4.7H2O, CaCl2, ferric citrate, MnSO4.H2O, phytic acid, L-proline, threonine, tetramethyl glutaric aciduria, gamma-aminobutyric acid, sodium nitroprusside, hydrocortisone, LaCl3, amino-oligosaccharin, chamomile leach liquor, and celery juice. A biocontrol bacillus seed solution is inoculated into the medium for temperature-variable fermentation culture, so as to promote the growth and reproduction of bacillus, and effectively stimulate the activity of bacillus, enhance the secretion of bacteriostatic substances and the activity of pathogen hydrolase, thereby significantly improving the antibacterial activity of bacillus to plant pathogenic fungi such as fusarium oxysporum, verticillium dahlia, botrytis cinerea, sclerotinia sclerotiorum, and providing a theoreticalbasis for the further development and application of biocontrol bacillus.

The invention relates to an application of a host defense inducer in preventing and treating selected bacteria pests in useful plants, wherein the selected bacteria pests are selected from acidovoraxavenae, burkholderia species, burkholderia glumae, phloem bacillus species, corynebacterium, erwinia species, pseudomonas syringae, pathogenic variant of pseudomonas syringa kiwi, pathogenic variant of pseudomonas syringe soybean, pathogenic variant of pseudomonas syringe tomato, pathogenic variant of pseudomonas syringe cucumber, streptomyces species, xanthomonas species, xanthomonas axonopodis,pathogenic variant of xanthomonas axonopodis citrus, pathogenic variant of xanthomonas axonopodis soybean, xanthomonas campestris, pathogenic variant of xanthomonas campestris banana, pathogenic variant of xanthomonas campestris peaches and plums, strawberry xanthomonas and semi-transparent xanthomonas. The invention also relates to a method for preventing and treating selected bacteria pests in useful plants by adopting the host defense inducer for treatment.

The invention discloses a culture solution for bacillus. The culture solution comprises the following components by mass percentage: 1.50-3.00% of a carbon source, 0.30-1.00% of a nitrogen source, 0.10-0.50% of a yeast extract or corn steep liquor powder or peptone, 0.10-0.30% of K2HPO4 or KH2PO4, 0.02-0.05% of MgSO4.7H2O, 0.01-0.02% of MnSO4.H2O, 0.05-0.10% of NaOH, 1.00-2.00 per thousand of a defoaming agent, 0.10%-1.00% of bacillus seed, and the balance water. The invention also discloses a preparation method of the culture solution and application of the culture solution in control of plant diseases and insect pests. Under a non-sterile airtight condition, the culture solution provided by the invention can realize on-the-spot small scale enlarged culture, the use effect is improved, and also the production cost is lowered.

The invention relates to a fermentation culture method for bacillus subtilis PTS-394. The fermentation culture method comprises the following steps of: inoculating a bacillus subtilis PTS-394 seed solution in a fermentation culture medium and carrying out fermentation culture for two days on a shaking table. The fermentation culture method is characterized in that a carbon source in the fermentation culture medium is corn starch, and a nitrogen source is soybean powder; three key factors affecting bacterial content predicated value are corn starch, soybean powder and yeast powder; the content of the three key factors in the fermentation culture medium is 11.48g / L, 13.35g / L and 0.90g / L, respectively; three key factors affecting the bacterial content and the inhibition zone diameter in the fermentation culture conditions are temperature, an initial pH value and liquid filling amount; and in the fermentation culture process, the temperature is 30.84 DEG C, the initial pH value is 6.93 and the liquid filling amount is 65.15mL / 250mL.

The invention relates to modified polynucleotides encoding modified proteases, and methods for altering the production of proteases in microorganisms. In particular, the modified polynucleotides comprise one or more mutations that encode modified proteases having modifications of the pre-pro region that enhance the production of the active enzyme. The present invention further relates to methods for altering the production of proteases in microorganisms, such as Bacillus species.