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Method for preparing calcium carbonate by directly extracting microbial strains from soil

A microbial strain, calcium carbonate technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of increased calcium carbonate yield, consumption, and multiple manpower and material resources, and achieve high separation efficiency.

Pending Publication Date: 2018-08-24
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese patent CN201610828703.2 discloses a method of using microorganisms to prepare calcium carbonate to solidify sand. The sand is poured into the bottle so that it evenly covers the bottom of the bottle, and then the gelling liquid and the cultured bacteria liquid are poured in, and the culture is shaken at a constant temperature. , and then quantitatively draw part of the mixed solution in the bottle every day, then add the same amount of gelling solution, evenly add a small amount of sand, and finally obtain a solidified sand column after several days; but the breeding of bacteria in this method usually consumes a lot of time Manpower and material resources, and the yield of calcium carbonate needs to be further improved

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  • Method for preparing calcium carbonate by directly extracting microbial strains from soil
  • Method for preparing calcium carbonate by directly extracting microbial strains from soil
  • Method for preparing calcium carbonate by directly extracting microbial strains from soil

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A method for directly extracting microbial strains from soil to prepare calcium carbonate, the main steps of the method are:

[0035] 1) Collect soil samples with an organic matter content of 20-30g / kg, take several soil samples within a depth of 5-30cm, put the soil samples into a sterilized sample bag, seal them and store them in a refrigerator at 4°C.

[0036] 2) Extract and isolate Bacillus species from soil samples.

[0037] a) Weigh 5g of soil samples respectively, pour the weighed soil samples into 90ml sterile water in the ultra-clean workbench, shake fully to make the soil samples suspend, and let stand at room temperature for 4 hours, which is 10 -1 Diluent.

[0038] b) Use a micropipette to take 0.5ml of the treated soil supernatant in a test tube filled with 4.5ml of sterile water, shake it, and let the bacteria solution mix evenly, which is 10 -2 Diluent. draw 10 -2 Dilute 0.5ml, transfer it into a test tube filled with 4.5ml sterile water, oscillate, l...

Embodiment 2

[0049]The same method as in Example 1 was used to extract and purify the Bacillus species in the soil to finally obtain the purified bacterial strain. The culture medium shown in Table 1 was prepared to propagate the isolated Bacillus strains; the strains obtained after 3 times of separation and purification were cultured for 36 hours, and the absorbance reached 1.5. Finally, the gel solution is prepared together with the strain solution to induce the strain to precipitate calcium carbonate. Mix equal volumes of bacterial solution and gelling solution (recipe in Table 3) into a 50ml centrifuge tube, place the centrifuge tube in a constant temperature air bath incubator at 30°C for 5 days, and filter the mixed solution to obtain microbially induced carbonic acid The calcium precipitation yield was 60%.

[0050] Table 3 gel formula (1000mL)

[0051]

Embodiment 3

[0053] Adopt the same method as in Example 1 to extract and purify the Bacillus species in the soil, the difference is that urea (20g / L) and nickel sulfate (20g / L) are added to the medium as a solid selective medium to finally obtain the purified bacterial strain. The culture medium shown in Table 1 was prepared to propagate the isolated Bacillus strains; the strains obtained after 3 times of separation and purification were cultured for 36 hours, and the absorbance reached 1.4. Finally, the gel solution is prepared together with the strain solution to induce the strain to precipitate calcium carbonate. Mix equal volumes of bacterial liquid and gelling liquid (recipe in Table 2) into a 50ml centrifuge tube, place the centrifuge tube in a constant temperature air bath incubator at 30°C for 5 days, and filter the mixed liquid to obtain microbially induced carbonic acid The calcium precipitation yield was 65%.

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Abstract

The invention belongs to microbial curing, and in particular relates to a method for preparing calcium carbonate by directly extracting microbial strains from soil. The method comprises the followingsteps: 1, extracting and separating out bacillus species from the soil with high organic matter content; 2, breeding and cultivating the bacillus species separated out in step 1; 3, mixing the bacillus liquid obtained in the step 2 with gelling liquid according to a equal volume proportion, putting the mixture into an air bath incubator at a constant temperature of 30 DEG C for curing for a periodof time, and then filtering the mixed liquid to obtain microbial induced calcium carbonate precipitate. The method can be used for preparing the calcium carbonate by directly extracting the microbialstrains from the soil; urea and nickel sulfate are selected as a solid selection medium for separating the bacillus species, so that the separation efficiency is high; the separated bacillus speciesare mixed with the gelling liquid, and the mixture is cured under the proper conditions, so that microbial induced calcium carbonate precipitate is obtained. The method is economical and environmentally-friendly, easy to operate and high in calcium carbonate yield.

Description

technical field [0001] The invention belongs to microbial solidification, in particular to a method for directly extracting microbial strains from soil to prepare calcium carbonate. Background technique [0002] Biomineralization is a very common natural phenomenon, and almost every kind of organism can synthesize minerals. Nearly two-thirds of it is calcium ore, and a considerable part has a cementing function. Through their own life activities, they are constantly enzymatically reacting with the surrounding environment medium to gradually form calcium carbonate. In recent years, with the research and development of microorganisms in soil, the possibility that the products of life activities (mainly activities of obtaining energy and carbon) of microorganisms that already exist in soil can be used in engineering has been widely recognized in concept. In such a big environment, microbial immobilization technology came into being. [0003] The so-called microbial solidific...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12P3/00C12R1/07
CPCC12N1/02C12N1/20C12P3/00
Inventor 缪林昌王呈呈孙潇昊童天志
Owner SOUTHEAST UNIV
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