2018 results about "Incubator" patented technology

A docking-station useful for providing a neonate predefined, continuous, stabilized and non-interrupted life-support environmental conditions, comprising of: (i) a neonate incubator, having at least one first opening and a life support system, and (ii) an imaging-device, having a scanning chamber with at least one second opening. The docking-station is configured such that said neonate incubator first opening and said scanning chamber second opening are juxtapose-able so as to reversibly hermetically communicate; thereby providing said neonate predefined, continuous, stabilized, non-interrupted life-support environmental conditions during the entire process of scanning.

An incubator with a double glazed wall that promotes regulation of sound pressure levels and temperature levels the incubator, using an insulated glass unit. The canopy of the incubator has at least one insulated glass unit. This unit includes at least one first inner glass and at least one second external glass. The inner and external glasses are spaced by a spacer, and are characterized by an inner atmosphere confined within the canopy and an external atmosphere.

The invention provides an automated apparatus of cell culture having tanks of culture medium, of growth factors and of cells to be cultured, an incubator having a thermostated enclosure which houses a cell culture vessel, and control computer system. A supporting and agitation device of the culture vessel is provided in the enclosure, and the culture vessel is formed by a bag having at least one inlet port connected to the tanks and one outlet port connected devices for harvesting and storage of the cells after culture, these harvesting and storage devices and tanks being located outside the enclosure and being connected to the cell expansion bag ports by conduits which together with the cell expansion bag form a preassembled module passing through a wall of the enclosure.

A portable livestock incubating device is provided formed of a rectangular container having and incubating volume forming an incubating chamber defined by the zone within the incubating volume. Each each end wall further includes an access door hingedly attached to an upper portion above a clean out door for providing ingress and egress of livestock. Each access door has an exhaust vent located at an upper portion whereby heated air, generated via an electric heater within said the incubating chamber exhausts therethrough. A base with wheels mounted thereon for facilitating transportation of the portable livestock incubating device.

An incubator (1) with an incubation chamber (2), a shaking device (4) which can be driven by a drive unit and which is used to shake receptacles (5) that can be placed in the incubation chamber (2) and that contain cell cultures, and a device chamber (3) which adjoins the incubation chamber (2) and which accommodates at least parts of the shaking device (4) protruding into the incubation chamber (2), wherein the shaking device (4) has a base plate (8) which seals off the incubation chamber (2) relative to the device chamber (4) and which, on its inner face (9) directed toward the incubation chamber (2), has a shaking table (11) that can be moved in a horizontal plane by a drive arm (10), wherein a motor (34) is arranged on the outer face (37) of the base plate (8) directed away from the inner face (9), which motor (34) drives a drive shaft (14) that is rotatably mounted in the base plate (8) and that is operatively connected to the drive arm (10).

The invention discloses a cold-storage transport incubator, which comprises a box body and a box cover matched with the box body, the inner walls of the box body and the box cover are provided with a polyurethane foam plastic layer, and the polyurethane foam plastic layer There is a layer of vacuum heat insulation board inside the layer, and the polyurethane foam plastic layer and the vacuum heat insulation board form a composite heat insulation structure. The thickness of the polyurethane foam plastic layer is 6-30 mm, and the thickness of the vacuum insulation panel is 5-10 mm. The surface of the polyurethane foam layer or the vacuum insulation board located on the inner wall of the incubator is covered with a protective layer. Adopting the cold storage type transportation incubator of the present invention, since the vacuum insulation board with excellent heat insulation effect is provided in the polyurethane foam plastic layer, the thickness of the polyurethane foam plastic layer can be greatly reduced, so that the heat preservation box with the same shape can The effective volume of the box is greatly improved, the weight of the incubator is also reduced to a certain extent, and the heat preservation effect is also better.

The invention relates to a coal rock porosity, permeability and electroacoustic stress-strain combined measuring device under overburden pressure and heating. The coal rock porosity, permeability and electroacoustic stress-strain combined measuring device is provided with an incubator, wherein a supporting rod, a comprehensive gripper, a pushing platform, a cushion block and a coal sample are arranged in the incubator, the upper part of the comprehensive gripper is provided with an experimental oil pipe, the experimental oil pipe is inserted into the coal sample, the upper part of the experimental oil pipe is sequentially connected with an electromagnetic valve e, a standard chamber, a pressure gauge b, an electromagnetic valve c, a barometric regulator and a gas booster pump, an inlet of the gas booster pump is communicated with a gas cylinder through a lead wire, and the upper part of the experimental oil pipe is sequentially connected with a pressure gauge c, an electromagnetic valve d, a water pressure regulator, a liquid booster pump and a water tank. The coal rock porosity, permeability, and electroacoustic stress-strain combined measuring device can be used for effectively simulating the high temperature and high pressure geological environment under deep complicated formation conditions to obtain the porosity, the gas-water relative permeability, a stress-strain curve, the resistivity and the acoustic velocity of the coal rock sample under the same experimental conditions, thus effectively saving the sample, improving the accuracy and the comparability of experimental data, and bringing the great convenience for scientific research.

The invention relates to a device and method for hatching the embryos of Chinese softshell turtles. The device comprises an incubator, an incubation plate and a collecting box and is characterized by further comprising a temperature sensor, a humidity sensor, a temperature control device, a humidifying device and a ventilation device. After the device is adopted, fertile eggs can be automatically hatched in a completely naked state; the device is high in hatching efficiency; and the defects that the fertile eggs are mildewed, the embryos die and the like caused by medium incubation in the prior art can be avoided.

The invention provides a single cell separation method based on droplet micro-fluidic chips. The method concretely comprises the following steps: A, a single cell suspension flows into a dispersed phase inlet channel from a dispersed phase inlet; B, an oil phase liquid flows into a continuous phase inlet channel from a continuous phase inlet; C, the above two phases merge to form droplets enclosing single cells, the droplets flow over a droplet capturing unit with the generation of a large number of droplets in a liquid storage pool, and stand for 2-5 min, and the superfluous droplets are sucked out when the droplets slowly settle into the droplet capture unit; and D, the droplet chips which capture the single cells are cultured in a 37 DEG C incubator, then DAPI is added to carry out nuclear staining, and the single cell capture rate is detected. The method has the advantages of simplicity and rapidity in operation, small use amounts of the cells and reagents, low experiment cost, high integration and wide application range.

The invention discloses a method for feeding lepidopteron. In the feeding process, a tray type net cage is used as a main feeding tool. The method comprises steps of collecting eggs, sterilizing, hatching larvae from the eggs, feeding the larvae, feeding pupas, feeding imagoes and the like, wherein gauze or plastic ropes are used as an oviposition substrate for collecting the eggs; the eggs are sterilized by using a formaldehyde solution with the volume concentration of 3-4%; the eggs are hatched and fed in an illumination incubator; the larvae are sterilized by using a sodium hypochlorite solution with the volume concentration of 8-12%; pupas feather in the tray type net cage; the feathering imagoes are continuously fed in the net cage and the eggs are collected and sterilized. The tray type net cage is simple and convenient to operate, the insects are effectively prevented from escaping and the cleaning can be carried out at any time; in the feeding process, eggs and larvae are disinfected by using different disinfectants, the disinfection effect is thorough and gentle, the bacteria are effectively prevented from mass propagation, and the hatchability of the eggs, the feathering rate of pupa are also improved, the hatchability of the eggs can be 95-96%, and the feathering rate of pupa can be 98%.

A method for identifying rice blast resistance of rice comprises the steps of scissoring three rice stems in a large bract period from a rice growing field, bringing the rice stems back to a laboratory, scissoring 2cm from the upper portion of each stem section and 5-6cm from the lower portion of each stem section to obtain one rice stem with the whole length of approximate 7-8cm, putting the three rice stems into culture dishes with two layers of filter paper, adding sterile water of 1mL into each culture dish, washing cultivated rice blast fungus spores with the sterile water, obtaining concentration of 106 pcs/ml, dropping spore suspending liquid of 8 muL to stem section sites of each rice stem through a liquid moving gun, putting the culture dishes into a illuminating incubator at the temperature of 26 DEG C, wrapping the culture dishes with transparent films to keep humidity in the culture dishes, cultivating for ten days in light and dark alternating modes to survey disease conditions, dividing survey disease levels into three levels, regarding the first level as disease-resistant cultivars, regarding the second level as susceptible cultivars, and regarding the third level as highly susceptible cultivars. The method optimizes the concentration and inoculation amount of inoculated rice blast fungus spores and establishes simple, convenient and accurate grading standards.

The invention discloses a morchella esculenta cultivating strain and an inducting method and a cultivating method therefor. An inducting culture medium of a morchella esculenta cultivating strain mother strain is used to induce morchella esculenta; a cultivating culture medium of a morchella esculenta cultivating strain is used to cultivate the morchella esculenta; the inducing method comprises the steps of taking out a preserved test tube strain; inoculating the test tube strain to an inducting culture medium flat plate; culturing in a culture box at a temperature of 18-22 DEG C; after hyphae grow on the whole plate, taking the hyphae as inoculating hyphae to be placed in the cultivating culture medium for use; and the cultivating method comprises the steps of sterilizing, inoculating and performing management on two stages of a mycelium growth period and a sporocarp growth period. The invention, through long-term test, searching and discussion, provides a morchella esculenta artificial cultivating technological method which has breakthrough at home and abroad; the morchella esculenta cultivating strain is an optimized and induced morchella esculenta strain; an integrated technology capable of maintaining stable production and continuous production is achieved; and commercial production is realized.

The invention discloses a method for testing the critical freeze damage temperature of a plant, and a system thereof, and belongs to the agricultural meteorological disaster monitoring and control field. The method comprises the following steps: putting a plant to be tested in a low-temperature constant-temperature incubator, stimulating natural frost conditions by using the low-temperature constant-temperature incubator, a speed adjustable fan and a humidifier to make the plant to be tested frozen, allowing one end of a working electrode to contact with the plant to be tested, connecting a high precision LCR tester by the other end of the working electrode, applying an AC excitation voltage signal to the working electrode, acquiring the electric signal of the plant by the working electrode, transmitting the electric signal to the high precision LCR tester, converting the electric signal into a digital signal, displaying, storing and processing in a computer for data analysis, and analyzing the change characteristics of the electric signal of the plant in order to obtain the freeze damage temperature of plant. The method and the system can be used for accurately measuring the critical freeze damage temperature of the plant, can be used in airflow disturbance frost prevention control, and provides important control parameters for the airflow disturbance frost prevention control.

The invention discloses separation, purification and identification methods of human amnion mesenchymal stem cells. The separation method of hAMSCs (human amnion mesenchymal stem cells) comprises the following steps: fragmentating human amnion; and carrying out two-step rotating digestion with trypsin of EDTA (ethylene diamine tetra-acetic acid) and collagenase of DNaseI, filtering with a steel mesh and collecting cell filtrate namely separated original hAMSCs. The purification method of hAMSCs comprises the following steps: incubating original hAMSCs with an LG (low glucose)-DMEM (dulbecco modified eagle medium) culture medium in a CO2 incubator; removing amnion epithelial cells which do not perform complete adherence growth under an inverted microscope; replacing a new culture medium on the third day; digesting with a trypsin-EDTA solution after cell converge degree reaches 80-90%; and collecting cells so as to obtain high-purity hAMSCs. The identification method of hAMSCs comprises the following steps: identifying hAMSCs and the amnion epithelial cells by adopting immunocytochemical staining vimentin and CK19; and detecting expressions of CD29, CD44, CD166, CD34 and CD45 by adopting a flow cytometry. The separation and purification methods disclosed by the invention have the advantages of high yield, high activity and high purity of hAMSCs; and the identification method is simple, convenient and precise.

The invention discloses a rapid cultivating method of sand moss crust, Including the steps that a whole piece of the moss crust in a sand natural environment of a dry area is collected, a silver leaf bryum is used as a dominant species, the impurities of sand and dead leaves are sieved to be removed after soaking; the moss crust is crushed after air drying in a shade place or oven drying for 48 hours under 30 degrees centigrade; the underlying sand of the moss crust is collected as substrate and is filled in a cultivating container with the diameter being 18cm, the height being 2cm and the thickness being 1.5cm, the moss pieces are evenly spread on the substrate surface inside the cultivating container, water is added until the water content is 13.9%; the moss pieces are put into a PGX multi-stage serial intelligent illuminating incubator to constantly cultivate for 40 days, and the moss crust can be formed. The cultivating condition requirement of the moss crust is relatively simple, the technology is convenient, the cost is low, and the method can be well popularized in production application.

The invention discloses a method for strawberry virus-removing, comprising the following steps: 1) putting the potted strawberry with virus into the bio-chemical incubator, culturing in the lethal temperature for virus for 2-3 days; 2) stripping the stem tip nascent tissue of smaller than 0.5mm, inoculating which in the culture medium with concentration of MS half-reduced for its growing; 3) transferring the stem tip nascent tissue when its sprout grows to 0.5-1.5cm in height to induced enrichment culture medium for growing; transferring it to biochemical incubator when generates adventitious bud, culturing under the lethal temperature for 30 minutes; 4) putting the sprout mentioned above into tissue culture room for culturing under 28 Deg C for 30 minutes; 5) repeating the steps 2) to 4) for 1-2 times. The method in this invention does not strip large quantity of stem tip and the virus-removing rate is as high as 100%.

The invention relates to a construction method of a human amniotic mesenchymal stem cell bank. The construction method comprises the following steps: taking a human amnion for detection, flushing and washing the human amnion with a phosphate buffered solution, then smashing the human amnion, diluting with the phosphate buffered solution, digesting with trypsin, digesting with collagenase IV and deoxyribonuclease I, and filtering to obtain a single-cell suspension; by adding human serum albumin, transferring, insulin and sodium selenite into a DMEM/F12 basal culture medium with the ratio of VDMEM to VF12 being 1 to 1, placing human amnion mesenchymal stem cells in an incubator under the serum-free condition, and then performing liquid exchange and culture transfer; subjecting the mesenchymal stem cells obtained through in vitro culture and proliferation to liquid nitrogen refrigeration, and preserving the cells according to the gender of newborn infants, the ABO/Rh type and the HLA type; establishing cell information files, so that the human amniotic mesenchymal stem cell bank is constructed. The method has the characteristics of no other animal derivation, wide source range and no ethic limitation. With adoption of the method, the human amniotic mesenchymal stem cells can be provided for cell therapy and other application.

The invention discloses a cell recognition and detection method based on a biochemical reagent of a supermolecule nanometer self-assembled system. The method comprises the following steps of: step one. a carbon nanotube or a graphite composite nanometer material is ultrasonically dispersed in a corresponding solvent so as to obtain a suspension liquid; step two. the suspension liquid prepared in the step one is uniformly and dropwise coated on the surface of an electrode, and the electrode is put in a dryer till a solvent volatilizes so as to obtain a nanometer self-assembled system modified electrode; step three. normal cells or tumor cells are washed and diluted with phosphate buffer, and then are uniformly coated on the surface of the nanometer modified electrode prepared in the step 2, the nanometer modified electrode is placed in an incubator at a constant temperature of 37 DEG C for 2h so as to obtain a cell sensor based on a nanometer interface; finally, by the using of the sensor, electroactive biochemical reagents such as tetrathiafulvalene derivatives or carborane derivatives and the like are used as probe molecules, and methods such as electrochemistry and surface contact angle detection and the like are used for carrying out high sensitivity detection and analysis on different types of cells.

The invention discloses a method for culturing bladder cancer organs in vitro. The method comprises the following steps: manufacturing a gas-liquid interaction culture system, namely manufacturing a uniformly paved rat tail collagen supporting layer on the surface of a porous culture membrane in a Transwell upper chamber; re-suspending fresh in-vitro bladder cancer tissues by using a rat tail collagen solution, uniformly mixing, adding the obtained mixture onto the rat tail collagen supporting layer, and then putting the rat tail collagen supporting layer into an incubator at 37 DEG C to solidify so as to obtain a rat tail collagen layer containing the bladder cancer tissues; adding an organoid culture medium into a Transwell lower chamber, and enabling the liquid level of the culture medium to be lower than the rat tail collagen layer containing bladder cancer tissues; and carrying out passage and cryopreservation. The culture success rate of the bladder cancer organoid is remarkablyincreased, the bladder cancer organoid with reserved immune cells can be obtained through culture, operation is easy, the utilization rate of tumor tissue is high, and great significance and value areachieved for bladder cancer drug screening research.