The invention provides a method for building a hollow vascularized heart based on the 3D biological printing technology and the hollow vascularized heart and relates to the technical field of
tissue engineering and
biology. The method includes: performing
reverse modeling to build a heart three-dimensional grid model, importing the data of the heart three-dimensional grid model into a double-
nozzle biological printing
machine, and driving the nozzles of the printing
machine to move according to a predesigned CAD digital model and selected forming parameters, wherein the
nozzle 1 of the printing
machine is loaded with sacrificial materials and used for printing a support framework, the
nozzle 2 of the printing machine sprays biological ink to obtain building bodies, and the effective components of the biological ink comprise hydrogel,
platelet-rich
plasma, third-generation human
umbilical vein endothelial cells and SD rat primary cardiomyocytes; crosslinking and cleaning the building bodies, and performing three-dimensional culture to form the hollow vascularized heart. The method has the advantages that the problem that a large-size hollow vascularized heart is hard in integrated printing, and the hollow vascularized heart built by the method is high in
cell activity and has certain functions.