157 results about "Injury repair" patented technology

The invention relates to methods and compositions for the differentiation of stromal cells from adipose tissue into hematopoietic supporting stromal cells and myocytes of both the skeletal and smooth muscle type. The cells produced by the methods are useful in providing a source of fully differentiated and functional cells for research, transplantation and development of tissue engineering products for the treatment of human diseases and traumatic tissue injury repair.

The present invention relates to pharmaceutical compositions comprising a chemotactic hematopoietic stem cell product comprising an enriched population of CD34+ cells containing a subpopulation of cells having chemotactic activity, methods of preparing these compositions and use of these compositions to treat or repair vascular injury, including infarcted myocardium.

The invention provides a method for manufacturing an artificial soft tissue body carried with a vascular net flow channel. The method comprises the following steps of: firstly designing a soft tissue scaffold model with a vascular structure, layering the soft tissue scaffold model one by one at equal distance, and manufacturing photomask plates of various layers; then uniformly mixing a cell and a collagen solution, and injecting a photocuring hydrogel and a photoinitiator to obtain a photocuring composite solution; injecting the photocuring composite solution to a work table, covering with the photomask plates, curing the photocuring composite solution by adopting a surface exposure technology, and then carrying out curing accumulation layer by layer to obtain a photocured hydrogel soft tissue scaffold with the vascular structure; planting vascular endothelial cells to the vascular structure of the photocured hydrogel soft tissue scaffold, wherein the vascular endothelial cells are attached to the surface of a vascular channel; carrying out static culture and dynamic culture in vitro to obtain the artificial soft tissue body carried with the vascular net flow channel. The method provided by the invention can solve the problems of survival of cells inside a large tissue engineering soft tissue scaffold, and soft tissue scaffold vessel network manufacturing and vascularization in the large injury repair of a soft tissue.

The invention discloses an osteochondral stent as well as a preparation method and application thereof. The osteochondral stent comprises a cartilage layer, an adhesion layer and a bone layer, wherein two sides of the adhesion layer are respectively connected with the cartilage layer and the bone layer; one or more of the cartilage layer, the adhesion layer and the bone layer are of a porous structure. The osteochondral stent can be used for repairing osteochondral defects, can fully capture cells, has a strong osteochondral injury repairing function, and is simple and convenient to operate.

The invention provides a personalized tissue repairing scaffold for bone, cartilage, skin injury and other injury repairing fields. The personalized tissue repairing scaffold is molded by means of a three-dimensional printing technology, and comprises a substrate and a plurality of sustained-release microspheres dispersed in the substrate, wherein each sustained-release microsphere is of a three-layer structure, namely a core layer, an intermediate layer and an outer layer, and growth factor and/or drugs are loaded on the core layer and outer layer of each sustained-release microsphere. According to the characteristics of treatment positions, degradable high-molecular compound hydrogel or degradable high-molecular/inorganic hybrid hydrogel can be further poured into holes of the substrate, and molding is achieved by means of a low-temperature freeze-drying method. The invention further provides a method for preparing the personalized tissue repairing scaffold. The personalized tissue repairing scaffold has excellent biocompatibility and degradability by adopting the sustained-release microspheres with three-layer structures, is used for pulsed sustained release of growth factor and/or drugs to achieve the effect of long-time stable sustained release, and is beneficial to prolonging of sustained-release cycle of growth factors and/or drugs.

The invention relates to a bionic stent simulating a natural tendon tissue fiber hierarchical structure and a preparation method of the bionic stent. The material is a multi-hierarchical structure simulating tendon tissue fibers. A double-nozzle spinning head is adopted, positive and negative electric fields are respectively added, nano fibers are received through a rotating funnel and twisted oncore layer yarns, and stable and continuous primary core-spun yarns are prepared. A plurality of primary core-spun yarns are stranded to serve as core layer yarns, nano fibers are electrostatically spun on the outer layers, the obtained secondary core-spun yarns are stranded to serve as a core layer, nano fibers are electrostatically spun on the outer layer, and tertiary core-spun yarns with a fiber multi-hierarchical structure simulating tendon tissues are prepared and used for a tendon repair stent. The bionic stent is beneficial to tendon repair and normal function recovery, is quite suitable to be used as a tendon injury repair and medical instrument material, and has good application prospect; the preparation method is simple and convenient; and the production efficiency is high.

The invention belongs to the field of medical materials for tendon injury repair and discloses a polycaprolactone/methylacryloyl elastin nanofiber composite membrane and a preparation method and application thereof. The preparation method specifically comprises the following steps of grafting methacrylic anhydride to elastin to obtain the methylacryloyl elastin; then mixing polycaprolactone with the methylacryloyl elastin uniformly to prepare a spinning solution; carrying out electrostatic spinning by using the spinning solution to obtain a composite membrane; and soaking the composite membrane by using a photoinitiator solution, carrying out photo-crosslinking under ultraviolet light and then carrying out washing and drying for many times to obtain the polycaprolactone/methylacryloyl elastin nanofiber composite membrane. The preparation method is easy to operate and relatively low in production cost, and the obtained composite membrane is good in mechanical property and biocompatibility and can be used for tendon tissue engineering and tissue repair.

The invention discloses a medicine-loaded nanoparticle vector and temperature-sensitive gel compounding system and a preparation method thereof. The preparation method includes the following steps that nanoparticle vectors are prepared; medicine molecules are connected with amino groups or hydroxyl groups at the ends of polyamidoamine dendrimers in a covalent bonding mode, and a medicine-loaded polyamidoamine dendrimer polymer is prepared; temperature-sensitive gel is prepared, the temperature-sensitive gel is a PLGA-PEG-PLGA copolymer, PLGA is a lactide-glycolide copolymer, and PEG is a polyethylene glycol copolymer; a copolymer solution is prepared by putting the PLGA-PEG-PLGA copolymer into a solvent; and under the condition of stirring, the medicine-loaded polyamidoamine dendrimer polymer is added into the copolymer solution, stirring is continued, and the medicine molecule-loaded nanoparticle vector and temperature-sensitive gel compounding system is obtained. The medicine-loaded nanoparticle vector and temperature-sensitive gel compounding system has a dual slow release function, and can stimulate synovial membrane mesenchymal cells to develop into cartilage cells, relieve the inflammation of a cartilage injured part, and promote the injury repair process of articular cartilages.

The invention relates to a machine-transplanted rice long-acting weed-closing and adverse-resistant green returning fertilizer granule, which comprises a compound fertilizer, an adhesive, a rooting agent, fulvic acid and a weed-closing agent. In the compound fertilizer, nitrogen, phosphorus and potassium are in a weight part ratio of 15:15:15, 15:10:10, or 19:7:14; the adhesive is pregelatinized starch, xanthan gum, sesbania gum, konjac gum, polyacrylamide, hydroxypropyl methyl cellulose, or carboxymethylcellulose; the rooting agent can be indoleacetic acid, indolebutyric acid, indole-3-butyric acid potassium, naphthylacetic acid or sodium naphthalene acetate; and the weed-closing agent can be butachlor, oxadiazon, pendimethalin, oxyfluorfen and the like. According to the invention, high polymer adhesive particles are prepared into a glue solution with clear water, compounding materials are crushed and then mixed evenly, and then the mixture is subjected to granulation molding by a double-roll extruding granulator, and the processing process is simple. The materials are prepared into dry granules that are rich in nutrients and can promote regulation, energy, and planting injury repair and the like, the granule are sprayed into rice field and become loose after water absorption. The granules provided by the invention can completely replace the fertilizers and herbicides produced by the traditional method.

The invention discloses a medicinal fatheadtree branchlet or bark alkaloid new compound with the function of promoting endothelial injury repair. The compound is prepared by a method including the steps: crushing medicinal fatheadtree branchlet or bark stems, adding water, performing soaking and extracting, collecting extraction liquid and performing vacuum concentration to obtain medicinal fatheadtree branchlet or bark extracts; extracting the extracts to obtain a dichloromethane portion and an ethyl acetate portion; feeding the ethyl acetate portion to a silica gel column; performing elution by taking dichloromethane-methanol with the volume ratio of 95:(5-10):90 as an elution solvent; combining similar portions by TLC (thin-layer chromatography) analysis; feeding a preparative liquid phase, taking methanol water as an eluent and performing liquid chromatography preparation to obtain the medicinal fatheadtree branchlet or bark new compound. Based on a cell repair related mechanism, under theoretical guidance, the proliferative activity of the medicinal fatheadtree branchlet or bark new compound is intensively studied, and experimental study indicates that the medicinal fatheadtree branchlet or bark new compound can obviously promote proliferation of HUVEC (human umbilical vein endothelial cell) cells and endothelial cell scratch repair and can be used for preparing a drug for controlling inflammatory endothelial injury.

The invention provides a wound protection composition, which is capable of preventing wound infection and promoting injury repair. The composition is free from antibiotics, and the composition consists of a dressing tenacity enhancing component, cell injury repair factors, a bactericidal-bacteriostatic molecule pair and a solvent. The composition can form a transparent liquid layer on the outer surface of a wound, and the transparent liquid layer can be rapidly transformed into a dressing which is thinner than 0.01mm. The composition is capable of forming a film easily and the film material is high in tenacity, and the dressing, which is formed on the outer surface of the wound, is high in air permeability and waterproof performance and is capable of effectively preventing such foreign matters as bacteria, dust and the like from invading into the wound so as to prevent infections; meanwhile, the dressing has such effects as killing bacteria, stopping bleeding, relieving pain and the like; and the composition is free from sensitization and hormone, and the composition is capable of effectively promoting the repair of damaged cells and wounds.

The invention relates to a tear drainage reconstruction system that can be regularly degraded. The reconstruction system is made by adopting polydioxanone (PDO) that is a degradable polymeric biomaterial. The system comprises an upper lacrimal duct part, a lower lacrimal dust part, a main lacrimal duct, a concave dacryocyst part and a nasolacrimal duct part. The system has good flexibility, certain elasticity, extensibility, breaking strength and compression strength performance and is a lamella micro-porous tubular structure that integrates stable nasolacrimal duct supporting function, 'tears pump' function, dacryocyst drainage function, 'drug control-release' function, the function of accelerating the fade-away of chronic inflammation of a lacrimal duct and the repairing function of mucous membrane injury of the lacrimal duct together. The reconstruction system has the advantages that an implanting method is a simple and convenient, the fixation in the lacrimal duct is the stable and reliable and the iatrogenic lacrimal duct injury can not appear, the regular degradation can be realized without secondary take-out and the reconstruction system is suitable for various lacrimal duct obstruction diseases and has high curative ratio of the lacrimal duct obstruction, especially, the reconstruction system has the stable and reliable medium-to-long term effect. The tears drainage reconstruction system currently is the only perfect material for high-degree lacrimal duct reconstruction that combines the physiological drainage of the tears with the pathological mechanism of lacrimal injury repair.