61 results about "Complex cell" patented technology

The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.

In a nonaqueous electrolyte cell-oriented electrode (10), an electrode active material layer (12) formed on a collector (1) has a density gradient developed with a gradient of a varied concentration of a solid along a thickness from a surface of the electrode active material layer (12) toward the collector (1), and in a gel electrolyte cell-oriented electrode (30), an electrode active material layer (32) formed on a collector (1) has a density gradient developed with (a) gradient(s) of (a) varied concentration(s) of one or both of an electrolyte salt and a film forming material along a thickness from a surface of the electrode active material layer (32) toward the collector (1).

The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.

The present invention provides an approach for the simultaneous determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of potentiators of cellular activation allows for characterization of such pathways and cell populations.

The present invention provides methods, instruments, reagents, kits and the biology involved in analyzing drug response. An embodiment of the present invention provides an approach for the characterization a plurality of pathways in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states of cellular molecules such as proteins, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Some of these categories include redox potential, ITIM phosphorylation, intracellular pH and other categories allows for characterization of such pathways and cell populations. Also, the present analysis is useful for the analysis of the effect of compounds on potential target cells.

The invention provides a multi-functional-region cell three-dimensional co-culture method based on a micro-fluidic chip. The chip is composed of a central main channel which is relatively low and side channels which are relatively high and are arranged on two sides, wherein the main channel and the side channels are connected by virtue of trumpet-shaped grid structures. Specifically, the method comprises the following steps: (1) adding collagen solutions different in composition to the sample inlet of the main channel of the chip and applying negative pressure to the outlet of the main channel, wherein the three collagen solutions flow into the main channel and functional regions, which keep distinct boundaries, are formed on the basis of a lamina flow principle; and (2) sterilizing the chip for a whole night through ultraviolet radiation, and directly blending cells to the collagen solutions which are injected into the main channel, so that cell inoculation can be achieved. By virtue of the micro-fluidic chip designed by the invention, a cell three-dimensional culture system, which has a plurality of independent functional regions, can be constructed in one step; and the method has an important application potential in the construction of in vitro complex cell micro-environments.

The method and system for psychological treatment by brain stimulation of the psychologically disordered object provide a possibility to stimulate the simple cells to assist the complex cells, and / or replace the complex cells in order to correctly perform the assigned command. An improved method includes the steps providing a creation of the psychoconduction code based on the simplified symbols, which by the audio, video, kinesthetic and / or olfactory stimulation simple cells to correct or replace the complex cells. An improved system comprises a controlling system comprising a processing system, including a controller and a memory, and a terminal device comprising at least one of a compact disk driver, a floppy disk driver, a printer and a control panel. Also, the improved system comprises an auxiliary equipment and a display connected to the controller, which is connected to the speaker(s).

The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.

A microfluidic system (12) for assembling and subsequently investigating complex cell arrangements has a three-dimensional microstructure (11) in which the cell culture is assembled, cultivated and investigated. At least two microchannel segments (16, 17) through which the microstructure (11) can be perfused from outside with a medium (21, 22) run in the microstructure (11), whereby the microchannel segments (16, 17) run at least in sections approximately parallel or equidistant to one another. The two microchannel segments (16, 17) are separated from one another by a wall structure (25) in which at least one aperture (26) connecting the two microchannel segments (16, 17) is provided. An electrode arrangement (27) is provided in or on the microstructure (11) in order to generate an inhomogeneous electric field (28) in the region of the at least one aperture (26). A cell arrangement is assembled in this microstructure (11) by supplying medium (21, 22) with cells (23, 24) for assembling the cell culture in the microstructure (11), with the inhomogeneous electric field (28) determined by the microstructure (11) bringing about the assembling of the cell arrangement from the supplied cells (23, 24).

The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell population.

The invention discloses a method for extracting a face feature by simulating a biological vision mechanism, and belongs to the field of image processing and pattern recognition. The method comprises the following steps of: simulating a learning mechanism of primary visual cortex simple cells, training a group of filters for describing a simple cell receptive field, and selecting a small number ofillumination invariant features of image extracted by the filter, which have the specific frequency selectivity, from the group of filters; simulating the function of primary visual cortex complex cells, and adding the expression and the translational invariance of the feature on the basis of the illumination invariance; strengthening a salient region of the invariant feature by using a visual attention mechanism; and converting the strengthened invariant feature into a characteristic vector for face recognition. An experiment shows that the method can effectively reduce the influence of illumination, expression and translation change on face recognition effect, and has real-time processing capability.

The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.

Methods for providing transgenic plants and parts hereof that, relative to the wild type state, is modified in a complex cell wall polysaccharide structure including pectins and hemicelluloses, the modification being in the overall glycosidic linkage pattern or the monosaccharide profile, comprising transforming a plant cell with a nucleotide sequence that causes an altered production of a complex cell wall polysaccharide-modifying enzyme such as endo-rhamnogalacturonan hydrolase, an endo-rhamnogalacturonan lyase, an endo-galactanase, an endo-arabinanase, an arabinofuranosidase, a galactosidase such as a beta-galactosidase, a xylosidase and an exo-galacturosidase. The modification can occur in vivo or post harvest, in which latter case the modifying enzyme is separated in the growing plant from its substrate, e.g. by targeting the enzyme to the Golgi, the endoplasmic reticulum or a vacuole, or is in a form that is inactive in the plant. After harvest the enzyme is brought into contact with its substrate or it is activated to provide the desired post harvest modification of the cell wall polysaccharide. The transgenic plant materials have improved functionalities and are useful in food and feed manufacturing and as pharmaceutically or medically active substances.

The invention discloses a cell division sequence detection method, belonging to the field of image analysis and mode recognition. The method comprises the following steps of: acquiring a first cell division candidate region by a cell division region distinguishing feature and space time correlation feature based method and extracting a cell division candidate sequence; describing the first cell division candidate region through a direction gradient histogram and converting the cell division candidate sequence into a characteristic vector sequence through characteristic extraction; and recognizing the cell division sequence through learning and deducing of a hidden conditional random field model according to the characteristic vector sequence. The method does not depend on experiential image processing; robustness and universality are improved by complex cell tracking and biological knowledge about specific cell morphology, behavior law and the like; and thus, the method can be widely applied to automatic understanding and detection of a cell behavior in a microscope image sequence.

The invention relates to a separation and efficient amplification culture method for an antigen specific T lymphocyte. By means of the immunomagnetic bead technology and the character of an active T cell expression CD137, the antigen specific T lymphocyte is separated from a complex cell mass; then an antigen specific T lymphocyte with high tumor killing activity is obtained through in-vitro efficient amplification culture. CD137L (CD137-ligand), IL-7, IL-15 and IL-2 are added in the culturing process, so that the phenomenon that activated and induced cells are liable to apoptosis in the culturing process is avoided, the amplification time is increased, and the killing activity of the antigen specific T lymphocyte is improved.

The invention discloses a high-whiteness bleaching method for high-yield bamboo wood pulp. The high-whiteness bleaching method comprises the following steps: firstly, using an extruder to carry out enhanced mechanical treatment on pretreated bamboo sheets; using a transition metal ion remover and the like to carrying out chemical impregnating treatment on extruded cizu materials; then, carrying out secondary spiral extrusion before pulping, straightening and screening; secondarily, using a peroxide activating agent and peroxide to carry out the activating treatment of 20 percent by weight of bamboo pulp at a treatment temperature of 60-90 DEG C for 30-60 minutes; thirdly, using a chlorine-free bleaching medicine H2O2 to carry out high-concentration rested bleaching of 10-30 percent by weight of bamboo pulp at a treatment temperature of 60-90 DEG C for 60-180 minutes; and using a decoloring agent of thiourea dioxide to decolour the bamboo pulp, remove a ground color, adjust a color phase and improve the bleaching white content. The invention forcefully and mechanically treats the bamboo sheets, synchronously realizes pulping, the removal of transition metal ions and deep extractionsand the break of complex cells, reduces the consumption of the bleaching medicine and improves the bleaching efficiency.

The invention discloses a cancer cell recognition method, device and system. The method comprises the steps of processing a cell image to obtain a first feature map; processing the first feature map to obtain a set; processing the set to obtain a second feature map, and processing the second feature map to obtain a cancer cell detection frame and a cancer cell code; processing the cancer cell detection frame is processed based on a deep learning network to output category information. According to the method, the cell image is processed to obtain the collection of the elliptical cell candidate frames, and the elliptical cell candidate frames can reduce background noise generated during subsequent processing compared with a normal rectangular frame; processing the set is processed to obtain a cancer cell detection frame and cancer cell codes, the segmenting cancer cells are segmented for type analysis, and reducing the subsequent image processing difficulty is reduced through the cancer cell codes; a cancer cell detection frame is processed to output type information, the type of cancer cells can be recognized through a mature deep learning network, and compared with an existing recognition mode, the cancer cell recognition method can better adapt to cancer cell recognition in a complex cell environment.

We have discovered a new method to analyze and characterize complex cell signaling networks. The method is based on specific binding of protein-protein interaction modules to a single type of protein or a mixture of proteins. The method utilizes a number of different protein-protein interaction domains as probes or sensors for the signaling state of the system under investigation.

The present invention provides an apparatus and a method to assemble organotypical tissues which can then be perfused and investigated under preferably physiological conditions. This is achieved by a microfluidic system for assembling and subsequently cultivating complex cell arrangements, comprising: a three-dimensional microstructure in which the cell arrangement is assembled and cultivated, at least two microchannel segments running in the microstructure and defining a flow direction, through which microchannel segments the microstructure can be perfused from outside with a medium, whereby the microchannel segments run approximately parallel or equidistant to one another at least in sections, a wall structure which separates the at least two microchannel segments and in which at least one aperture connecting the at least two microchannel segments is provided, and an electrode arrangement provided in or on the microstructure in order to generate an inhomogeneous electric field in the region of the at least one aperture.

The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations.

The invention provides a three-dimensional complex cell aggregate model as well as a preparation method and application thereof. According to the model, a natural or chemically synthesized polymer is used as a matrix material to prepare submicron-scale microspheres, and cell factors are embedded in or fixed on the surface of the submicron-scale microspheres and are co-cultured with cells to form a complex cell aggregate containing the microspheres, so that the biological activity of the cells in the aggregate can be regulated and controlled from inside to outside. The model can be used for researches of propagation and differentiation of stem cells in a three-dimensional state, overcomes the problems that the cell aggregate is heterogeneous in composition / structure, the cell proliferation efficiency is low and the directional induced differentiation efficiency is low due to non-uniform mass transfer and short acting time of liquid factors in a culture medium during the traditional three-dimensional culture of the stem cells, can be used for stimulating interaction between cells and cells, cells and an extracellular matrix as well as cells and soluble factors, thereby providing a theoretical and technical support for in vitro construction and optimization of a stem cell culture technology, and tissue engineering construction of various tissue and organ analogs or equivalents.

Provided is a method for inter-cell interference management in a cellular system. More particularly, provided is a method for inter-cell interference avoidance that may not require signaling between base stations and a complex cell planning.

The invention relates to a feature extraction method based on biological vision. The method comprises the steps that multi-direction multi-scale Gabor filtering is carried out on an original input image to obtain a set of responses of the original input image, and the responses are used for simulating locality and direction sensitive features of a simple cell receptive field of a visual cortex; the maximum response value and the direction corresponding to the maximum response value of each position of the responses are obtained, and are used for simulating invariance extraction features of a complex cell receptive field of the visual cortex; the responses are divided into a plurality of square blocks equal in size, histogram statistic is carried out in the direction with the square blocks as the unit, and statistics is carried out on each position in the maximum response value direction of each position to reflect overall space distribution performance of the features; the accumulation and standardization operation are carried out with the square blocks as the unit to obtain a set of feature vectors with selectivity and invariance. The method simulates the features of the simple cell receptive field and the complex cell receptive field of the visual cortex, low-dimension and effective features are obtained for images of complex scenes, and the method is used for the field of object identification, detection and tracking.

The invention discloses a blood leucocyte separating medium and application thereof, a preparation method of the blood leucocyte separating medium, and a blood leucocyte separating vacuum blood collection tube with the blood leucocyte separating medium and application thereof. The blood leucocyte separating medium comprises glucan T500, hydroxyethyl starch, meglumine diatrizoate, water, sodium citrate, NaN3, poly(diallyldimethylammonium chloride) and polyvinyl pyrrolidone, the proportion of parts by weight of which is 18.5-21.5:1.5-2.5:2-3:69:3.8:0.1:0.05-0.15:2-3. The invention has the advantages that clinical blood collection and anticoagulant leucocyte seperation can be completed at a time, the sodium citrate in the blood leucocyte separating medium and calcium ions in blood are chelate and anticoagulant, the technical problem of damaging the liquid level layer of the separating medium does not exit, a supernatant liquor is sucked up without needing centrifugation after natural precipitation, a complex cell seperation technology is transformed into a simple blood collection seperation process, the processes are easily united, the repeatability of test results is good, and the quality controllability is strong.

A laminated structure of a redox flow cell, an integrated complex electrode cell, and a redox flow cell comprising same, wherein the integrated complex cell can reduce stack lamination process time and lamination cost and increase lamination efficiency by integrating a manifold and a bipolar plate in order to facilitate lamination. The integrated complex electrode cell having an inner seal structure, which inhibits the overflow of electrolytes, is characterized in that it inhibits the overflow of electrolytes of positive and negative poles by forming a structure in which an integrated part of the manifold and the bipolar plate can be sealed.

The invention discloses a method for evaluating the quality of a stereo image based on binocular information processing. The method comprises the steps that simple cell information matrixes are obtained through complex wavelet transform and complex cell information matrixes are formed through collection of coefficients of complex wavelet transform; on the basis of the complex cell information matrixes, two aspects are evaluated, in the first aspect, the binocular complex cell information matrixes are blended, so that a binocular blended image is obtained, and according to the fact that the human eyes has different degrees of visual sensitiveness to binocular information in different frequencies, the evaluated quality of the binocular blended image is finally obtained; in the second aspect, binocular stereo perceptual information is extracted from each complex cell information matrix, with the consideration of the stereoscopic parallax angle and with the combination of the binocular masking characteristic, the human eyes can be influenced by the background luminance, the texture mask, the space contrast ratio and the like in the visual perception process, and the evaluated quality of stereo vision perception is obtained; finally, with the combination of the evaluations obtained from the two aspects, a final stereo image quality evaluation result is obtained and the uniformity of the evaluation result and the subjective perceptive performance of the human eyes is good.

The invention relates to an in-vitro multicellular line coculture dish and a manufacturing method thereof. The in-vitro multicellular line coculture dish comprises a shell (1). The in-vitro multicellular line coculture dish is characterized by also comprising a diaphragm (2) and a partition (3), wherein the diaphragm (2) is arranged in the shell (1) and divides the shell (1) into a plurality of chambers; the partition (3) is detachable and is arranged beside the diaphragm (2); and the diaphragm (2) is a polycarbonate film. The in-vitro multicellular line coculture dish has simple structure, and can maximally simulate the living environment of complex cells in vivo. By using the in-vitro culture dish, multiple cells, which may interact with each other, can be cultured in the same environment; and the cells do not contact with each other, but cell factors secreted by the cells can interact with each other, thereby simulating an in-vivo physiological environment in vitro and completing the positive and negative feedback effects among the cells. The manufacturing method is simple and has the advantages of low investment and low cost.

In a method and apparatus for analyzing multivariable data sets, a general computerized platform is provided for evaluating the relationship between large number of measurements of sets of variables characterizing components of complex states of a system under induced stimulation or controlled conditions. The linked responses of variables and their temporal relations tell about the network of interactions and their hierarchy. Processing of data sets by a simple neural network gives a matrix of weight parameters, that allow to identify fingerprints of complex states characterized by patterns of measured variable and estimate the interactions between the components characterized by the measured variables. The results are provided numerically and by color-coded presentation indicating dominating relations between variables and strongly responding variables. When applied to dynamic responses of a system, the analysis can construct a schematic hierarchical architecture of the network of interaction between the components of the studied system. Applications in biology include analysis of measurements characterizing responses of molecular components in cells under changes induced by stimuli (e.g. drugs, growth factors, hormones, mutations or forced expression of a proteins), and identification of complex cellular states (e.g. proliferation, differentiation, transformation, starvation, necrosis, apoptosis, and the time dependencies of the above effects).

A method and system is described for mapping a system-level description of an integrated system directly to a technology-specific set of logic cells that are comprised primarily of large complex cells (bricks). The invention is based on applying aggressive Boolean operations that would be of impractical runtime complexity for a large library, but are applicable for the targeted brick libraries which typically contain a small number of complex cells, along with a much smaller number of simple cells. This invention is modular such that it can be applied in the context of incremental netlist optimization as well as optimization during physical synthesis.

A method and system is described for mapping a system-level description of an integrated system directly to a technology-specific set of logic cells that are comprised primarily of large complex cells (bricks). The invention is based on applying aggressive Boolean operations that would be of impractical runtime complexity for a large library, but are applicable for the targeted brick libraries which typically contain a small number of complex cells, along with a much smaller number of simple cells. This invention is modular such that it can be applied in the context of incremental netlist optimization as well as optimization during physical synthesis.