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474 results about "Cell morphology" patented technology
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Cell morphology. Cell morphology is essential in identifying the shape, structure, form, and size of cells. In bacteriology, for instance, cell morphology pertains to the shape of bacteria if cocci, bacilli, spiral, etc. and the size of bacteria. Thus, determining cell morphology is essential in bacterial taxonomy.
Prediction of prostate cancer progression by analysis of selected predictive parameters
InactiveUS6025128AImprove performanceMicrobiological testing/measurementBiological testingStatistical analysisFactor ii
A method for screening individuals at risk for prostate cancer progression is disclosed. The method is useful for evaluating cells from patients at risk for recurrence of prostate cancer following surgery for prostate cancer. Specifically, the method uses specific Markovian nuclear texture factors, alone or in combination with other biomarkers, to determine whether the cancer will progress or lose organ confinement. In addition, methods of predicting the development of fatal metastatic disease by statistical analysis of selected biomarkers is also disclosed. The invention also contemplates a method that uses a neural network to analyze and interpret cell morphology data. Utilizing Markovian factors and other biomarkers as parameters, the network is first trained with a sets of cell data from known progressors and known non-progressors. The trained network is then used to predict prostate cancer progression in patient samples.
Owner:CYTODIAGNOSTICS +3
Pluripotent stem cells derived without the use of embryos or fetal tissue
InactiveUS20030113910A1New breed animal cellsArtificial cell constructsPluripotential stem cellGerm layer
Owner:STEMA
Diazonium salt modification of silk polymer
ActiveUS20090232963A1Change physical propertiesPeptide/protein ingredientsPharmaceutical containersChemical MoietyPolymer science
A method for modifying silk polymer by coupling a chemical moiety to a tyrosine residue of a silk polymer is described herein for the purpose of altering the physical properties of the silk protein. Thus, silk proteins with desired physical properties can be produced by the methods described herein. These methods are particularly useful when the introduction of cells to a mammal is desired, since modifications to the silk protein affect the physical properties and thus the adhesion, metabolic activity and cell morphology of the desired cells. The silk protein can be modified to produce, or modify, a structure that provides an optimal environment for the desired cells.
Owner:TRUSTEES OF TUFTS COLLEGE TUFTS UNIV
Cell fixation and use in phospho-proteome screening
InactiveUS7326577B2Expand accessEasy screeningWithdrawing sample devicesChemiluminescene/bioluminescenceEpitopeWilms' tumor
Owner:LAB OF AMERICA HLDG
Method and device for classifying white blood cells
ActiveCN103745210AAccurate classificationCharacter and pattern recognitionMaterial analysisWhite blood cellSample image
The invention provides a method and a device for classifying white blood cells. The method comprises the following steps: dyeing the white blood cells in a blood sample to obtain a blood sample containing the dyed white blood cells; performing image acquisition on the white blood cell blood sample to obtain a white blood cell blood sample image; segmenting various cells of the white blood cell blood sample image, and respectively extracting cell morphology characteristic parameters of various cells; performing re-segmentation on various segmented cells to obtain a cell nucleus image and a cytoplasm and particle image; extracting color characteristic parameters of the cell nucleus image; extracting particle distribution characteristic parameters and color characteristic parameters of the cytoplasm and particle image; performing normalization on extracted characteristics; sending the normalized characteristics into a neural network classifier, identifying five types of cells in the white blood cells, and respectively working out the number of the five types of cells and the percentage of total white blood cell count. According to the method and the device for classifying the white blood cells provided by the invention, the white blood cells can be accurately classified.
Owner:AVE SCI & TECH CO LTD
Methods of detecting cancer cells in biological samples
InactiveUS20040197839A1Preparing sample for investigationBiological testingBladder cancerBacteriuria
The invention provides methods of detecting cancerous cells in biological samples using a double staining / dual imaging approach, which can be used to diagnose cancer. More specifically, the present invention provides methods of diagnosing bladder cancer by a simultaneous scanning of cell morphology and FISH signals of cells derived from a urine sample.
Owner:BIOVIEW
Method for displaying virtual slide and terminal device for displaying virtual slide
ActiveUS20060050948A1Convenience to workGuaranteed to workCharacter and pattern recognitionMaterial analysisOphthalmologyVirtual slide
The invention relates to a method for displaying a virtual slide capable of storing positional information of a prescribed cell in a virtual slide photographed with a magnification capable of recognizing cell morphology and a terminal device for displaying the virtual slide; or a method for displaying the virtual slide capable of classifying the prescribed cell in the virtual slide photographed with a magnification capable of recognizing cell morphology by a simple operation; or a terminal device for displaying the virtual slide.
Owner:SYSMEX CORP
Method of forming thermoplastic foams using nano-particles to control cell morphology
ActiveUS20050112356A1Low thermal conductivitySmall cell sizeMaterial nanotechnologyLayered productsPolymer scienceHigh pressure
A process for making closed-cell, alkenyl aromatic polymer foams using nano-particle nucleation agents to control the cell morphology of the resulting foam includes forming a polymer melt at a temperature above the polymer glass transition temperature (for crystal polymers) or the polymer melt point (for amorphous polymers); incorporating selected nano-particles into the polymer melt; incorporating blowing agents into the polymer melt at an elevated pressure; optionally incorporating other additives, such as flame retardants, into the polymer melt; and extruding the polymer melt under conditions sufficient to produce a foam product having a desired cell morphology, characterized by parameters such as reduced average cell size range and / or increased asymmetry of the cells.
Owner:OWENS CORNING INTELLECTUAL CAPITAL LLC
Method for displaying virtual slide and terminal device for displaying virtual slide
ActiveUS7925070B2Convenience to workGuaranteed to workCharacter and pattern recognitionMaterial analysisOphthalmologyVirtual slide
Owner:SYSMEX CORP
Classification method and system for cancer digital pathological cell image
ActiveCN106127255AOvercoming diversityOvercome irregularities and many other problemsCharacter and pattern recognitionLearning machineClassification methods
The invention provides a classification method and a classification system for cancer digital pathological cell images. According to the classification method and the classification system, a suspected lesion region of interest is subjected to block processing, the suspected lesion region after block processing is subjected to feature extraction by utilizing partial matching pattern textural features, and the extracted features are classified and identified by adopting an extreme learning machine training method, so as to determine benign and malignant tumors and differentiate levels. The classification method and the classification system for the cancer digital pathological cell images utilize the partial matching pattern textural features for conducting feature extraction, analyze the textural features of cells from macroscopic and microscopic aspects, have the advantage of rotation invariance, effectively overcome the problems of diversity, irregularity and the like of cell morphology, provide reliable textural feature information for classification, apply an extreme learning machine to the classification of breast cancer cells, shorten the training time, accelerate the speed of classification and identification, and improve the accuracy of recognition.
Owner:SHENZHEN INST OF ADVANCED TECH
Improved mesenchyme stem cell protection solution and application thereof
ActiveCN101919380ACharacteristics unchangedMeet application needsDead animal preservationMesenchymeCell activity
The invention relates to an improved mesenchyme stem cell protection solution as well as application and a preparation method thereof. The protection solution can effectively prolong the activity remaining time of mesenchyme stem cells, reduces preparation cost, and has the advantages of wide raw material source, simple preparation, safe and reliable direct clinical application; and after the mesenchyme stem cells are preserved for 48 hours in the protection solution, the cell activity is still above 90 percent, the cell morphology is normal, and the multiplication capacity and mesenchyme stem cell phenotype characteristics are not influenced.
Owner:青岛奥克生物开发有限公司
Preservation solution and preparation method and application thereof
InactiveCN109122667ALong storage timeImpaired inhibitionDead animal preservationIonic strengthAnimal feces
The invention provides a feces preservation solution, a preparation method and an application thereof and a feces preservation method, and belongs to the technical field of biological agents. The feces preservation solution is prepared from the following components: a chelating agent with concentration of 10-500 mmol / L, a bacteriostat with concentration of 100-600 mL / L, an ionic strength maintainer with concentration of 1-10 g / L and a denaturant with concentration of 1-50 g / L. The feces preservation solution can preserve feces samples at normal temperature, and can effectively inhibit DNA degradation and cell damage in feces samples and maintain cell morphology, and has better effect of inhibiting activity of degrading enzyme and microorganisms, and preservation time of the samples is long.
Owner:广州维帝医疗技术有限公司
Cell three-dimensional mechanical loading unit
InactiveCN101298592AIncrease stretch areaIncreased adhesion areaTissue cultureElectrical/wave energy microorganism treatmentSilicone membraneMagnetic bead
The invention discloses a drawing and loading device for cells, which relates to a cellular mechanics loading device that belongs to the medical instrument. The drawing and loading device for cells consists of a control part, a mechanical part and an electromagnetic part, which is characterized in that: the cells are cultivated on an elastic silicon membrane, and circumferential-direction drawing and compressive loading are carried out for the cells through a dynamic system, meanwhile, magnetic bead collagen with ferroferric oxide enveloped is attached to the surfaces of the cells through integrin family, then alternating magnetic field is respectively loaded at an upper and a lower bottoms of a cell cultivating box, rendering the cells to be drawn under the magnetic force while being under the circumferential stress in a base strain field, so as to control the transformation of the cells along the axis Z and control the cell morphology. By simulating the borne mechanical stress of cells under physiological conditions, the drawing and loading device for cells can realize mechanical loading with different dimensions under different mechanical forces, and has the advantages of simple structure, convenient use, wide application and excellent reproducibility, etc.
Owner:CHONGQING UNIV
High content image flow biological microscopic analysis system
The invention provides a high content image flow biological microscopic analysis system. The system comprises a liquid flow system and an optical system, wherein the liquid flow system enables cells in sample cell suspension to be focused in the center of a liquid flow under the constraint of the system sheath liquid flow and to flow through a detection window of a flow chamber one by one; the optical system comprises optical sources, an optical path system and an optical filter stack; the optical sources include a halogen lamp and a laser; through the optical path system and the optical filter stack formed by a plurality of dichroscopes, the optical system generates a bright field cell image, a six-channel fluorescent cell image and six PMT (photomultiplier tube) optical intensity signals with different wave bands. The high content image flow biological microscopic analysis system integrates flow cytometry and fluorescence microscopic imaging, has a plurality of detection channels, can collect the cell images of the cells passing through the flow chamber one by one and can carry out quantitative analysis on each cell image by utilizing analysis software, thus providing the statistical data of a cell group and the information of the cell morphology, cell structures and subcellular signal distribution.
Owner:SHANGHAI JIAO TONG UNIV
Automatic blood cell recognition device and operating method thereof
The invention provides an automatic blood cell recognition device which comprises a laser light source part, a microscope acquisition part and an objective table part which are respectively connected with a computer. The blood cells can be automatically recognized without staining or utilizing an antibody / antigen reaction on the cell surface, and the blood cell recognition is realized by utilizing cell morphology and spectral characteristic change according to a hyperspectral image. The system is a human blood cell recognition technology with the technical characteristic of automatic non-destructiveness.
Owner:SHANDONG UNIV
Tissue chip used for tumour early stage diagnosis and preparation device
Three kinds of tissues including cancer tissue, precancerosis and corresponding normal tissue are sliced up, dyed, marked, and positioned. Receptor holes are prepared by leading designed lattice array mould paper to paste on surface of wax block of receptor. Wax block with tissue core bar is prepared by using perforating needle and puncture needle for tissue. Common cancer such as lung cancer, nasopharyngeal carcinoma, oesophagus cancer etc. and having integrated clinical data and pathology features are selected. Through in situ hybridization, testing mRNA of relevant gene and expression of protein on tissue chip, consistent result between the invented product and traditional test is validated. In the product, cellular morphology is clear and even, and there is no fallen off tissue point. The invention is applicable to filter cancers, early diagnosis and forecasting prognosis.
Owner:中南大学湘雅医学院肿瘤研究所
Induction and in-vitro culture method of plant stem cells derived from apical meristem of catharanthus roseus roots
InactiveCN111876368AStable subcultureNo epigenetic variationCell culture mediaPlant cellsBiotechnologyStem cell line
The invention discloses an induction and in-vitro culture method of plant stem cells derived from apical meristem of catharanthus roseus roots. The induction method comprises the following steps: cutting off a root cap from the root tip of the catharanthus roseus, performing inoculating to an induction culture medium, performing culturing in dark, and proliferating and growing a stem cell layer inan explant to form a cell cluster; transferring the layered stem cell layer to a subculture medium for culturing for 10-15 days, and performing complete culturing in dark; and performing transferringto a new subculture medium, and performing culturing for 10-15 days under the same condition to obtain a catharanthus roseus stem cell line with stable cell morphology. A catharanthus roseus stem cell culture system is established by inducing apical meristem of the catharanthus roseus roots, stable subculture can be performed under certain conditions, and epigenetic variation is avoided. The catharanthus roseus stem cell culture system established by the invention has the characteristics of being high in growth speed, high in genetic stability and the like, and culture with a 10L bioreactor is preliminarily realized.
Owner:LINGNAN INST OF TECH
Cell division sequence detection method
InactiveCN102156988AImprove robustnessImprove versatilityImage analysisConditional random fieldAlgorithm
The invention discloses a cell division sequence detection method, belonging to the field of image analysis and mode recognition. The method comprises the following steps of: acquiring a first cell division candidate region by a cell division region distinguishing feature and space time correlation feature based method and extracting a cell division candidate sequence; describing the first cell division candidate region through a direction gradient histogram and converting the cell division candidate sequence into a characteristic vector sequence through characteristic extraction; and recognizing the cell division sequence through learning and deducing of a hidden conditional random field model according to the characteristic vector sequence. The method does not depend on experiential image processing; robustness and universality are improved by complex cell tracking and biological knowledge about specific cell morphology, behavior law and the like; and thus, the method can be widely applied to automatic understanding and detection of a cell behavior in a microscope image sequence.
Owner:TIANJIN UNIV
Rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof
ActiveCN106591168AResidue reductionReduce production and use costsBacteriaWater contaminantsAntibiotic YBiology
The invention discloses rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof. The invention provides a strain namely rhodococcus ruber YMHL-1 capable of degrading residual nicosulfuron in wastewater, and the identification shows that the strain belongs to Rhodococcus. The strain is preserved in China General Microbiological Culture Collection Center, CGMCC in February 9th, 2015; and the preservation number is CGMCC No. 10542. The main biological characteristics are as follows: the Gram staining reaction is negative; the bacterial colony is in an orange yellow color, is in a round shape, and is opaque, the surface of the bacterial colony is rough and dry; the cell is in a sphere shape or short bar shape, does not have any flagellum, is motionless, and does not generate any spore; the strain is aerobic and chemoheterotrophic and cannot liquefy gelatin and hydrolyze starch; the enzyme contact reaction is positive, the V.P. reaction is positive, the methyl red reaction is negative; the strain cannot degrade casein, cannot reduce nitrates; and the strain can produce acids from glucose, can utilize glucose, fructose, and citrates, cannot utilize mannose, arabinose, and raffinose, and can reduce more than 90% of antibiotics in water through direct application.
Owner:JIANGSU NANZI ENVIRONMENTAL PROTECTION SCI & TECH
Method for discriminating non-blood-borne nucleated cells enriched from human or animal biological fluid
ActiveCN103091491AHigh recovery rateAccurate identificationMicrobiological testing/measurementPreparing sample for investigationHuman bodyStaining
Owner:有限会社林平
Method for efficiently inducing reprogramming of human body cells into pluripotent stem cells
ActiveCN102093981AImprove the efficiency of inducing iPSCsShort induction periodVector-based foreign material introductionForeign genetic material cellsNuclear reprogrammingExpression gene
The invention provides a method for efficiently inducing reprogramming of human body cells into pluripotent stem cells, belonging to the field of biomedicine. The method provided by the invention comprises the following step: before carrying out nuclear reprogramming on body cells, culturing the body cells for 2-4 days in a culture medium containing thyroid hormone T3. The method provided by the invention can increase the iPSC (induced pluripotent stem cell) induction efficiency by 20-100 times to about 1.2%, and shorten the induction cycle to 12-16 days; and meanwhile, different clones of the iPSC obtained by induction have uniform cellular morphology, multiplication capacity and pluripotent gene expression, and can be used for researching human diseases.
Owner:SHENZHEN BEIKE BIOTECH
Zaralenone degrading bacteria and application thereof
InactiveCN106047749AActive part of degradationBacteriaAnimal feeding stuffBacteroidesBacillus amyloliquefaciens
The invention discloses zaralenone degrading bacteria and application thereof, and belongs to the technical field of biology. ZEN is used as a unique carbon source and energy to carry out preliminary screening and secondary screening, a bacteria strain H6 (CCTCC NO: M2016129) capable of degrading ZEN effectively is separated out, fermentation liquor of the bacteria H6 and ZEN (with final concentration of 10 (mu)g / mL) are cultured for 72 hours under the conditions of 37 DEG C and 180 r / min, and the degradation rate to ZEN reaches 93.64%. Through cellular morphology and physiological and biochemical identification and 16 S rRNA sequence comparative analysis, the strain of bacteria is identified as the bacillus amyloliquefaciens. The degradation active substances of the bacteria strain H6 mainly exist in fermented supernate, thalli also have partial degradation effect. The bacteria strain H6 can be used for preparing a microorganism preparation for degrading zaralenone or a feed biodegradation additive.
Owner:HENAN AGRICULTURAL UNIVERSITY
Quantitative analysis method aiming at apoptotic cell morphology of fluorescence microscopic image
InactiveCN103994964AFast splitReduce subjective factorsIndividual particle analysisMicroscopic imagePattern recognition
The invention relates to a quantitative analysis of an apoptotic cell morphology, which can effectively divide cell individuals from an image, automatically calculate cell two-dimensional morphology parameters and greatly shorten image analyzing time. The technical scheme adopted by the invention is a quantitative analysis method aiming at the apoptotic cell morphology of a fluorescence microscopic image. The quantitative analysis method comprises the following steps: (1) collecting a plurality of pairs of fluorescence microscopic cell images to be treated; (2) applying median filtering to a light microscope image in one pair of the images; carrying out gray stretching and carrying out image pre-treatment by using morphology opening operation; (3) automatically finding a threshold value by using an Otsu algorithm; (4) obtaining a divided binary image; (5) filling holes by using a morphology expansion and corrosion method, and removing small regions and incomplete targets on the boundary; (6) judging the state of a cell by the divided binary image according to a dyeing result; (7) outputting a parameter calculation result of each cell. The invention is mainly applied to the quantitative analysis method for the apoptotic cell morphology.
Owner:TIANJIN UNIV
Method of forming thermoplastic foams using nano-particles to control cell morphology
InactiveCN1886449AHigh mechanical strengthDecrease in median cell sizeMaterial nanotechnologyThin material handlingPolymer scienceGlass transition
Owner:OWENS CORNING INTELLECTUAL CAPITAL LLC
Cryopreserving solution for clinical-grade CAR-T cryopreservation and capable of being directly reinfused through intravenous infusion
InactiveCN108552159AAvoid lostReduce workloadInorganic non-active ingredientsPharmaceutical delivery mechanismClinical gradeHydroxyethyl starch
The invention discloses a cryopreserving solution for clinical-grade CAR-T cryopreservation and capable of being directly reinfused through intravenous infusion. The cryopreserving solution comprisesthe following raw materials: dimethyl sulfoxide, a glycerol fructose sodium chloride injection, an invert sugar electrolyte injection, a dextran 40 glucose injection, a hydroxyethyl starch 130 / 0.4 electrolyte injection, a vitamin C injection, a human serum albumin injection and a 0.9 % sodium chloride injection; the cryopreserving solution has the pH value of 6.8-7.0. When a formula for the clinical-grade CAR-T cryopreservation and capable of being directly reinfused through the intravenous infusion is used for cryopreserving CAR-T cells, the thawed cells and the thawed cryopreserving solutiondo not need centrifugation, resuspension, fluid exchange and other processes and can be directly reinfused through the intravenous infusion, so that the loss of cell varieties due to pollution in-vitro repeated proliferation of the CAR-T cells, or changes of cell morphologies and cell functions are effectively avoided, medical equipment can be also significantly simplified and the workload of medical workers can be reduced.
Owner:WUHAN BIO RAID BIOTECH CO LTD
Screening method for anaerobe capable of degrading chlorophenol
InactiveCN1827765AObservation and trainingObserve the growthBacteriaTreatment with anaerobic digestion processesSludgeAnaerobic bacteria
The method uses the anaerobic glove case and many modified culture medium to screen the anaerobic bacteria which possesses the highly effective character of degrading chlorophenol and acid resistance. The method comprises the following steps: A. screening the anaerobic particle sludge; B. segregation and purifying bacteria; C. primary screening the anaerobic bacteria degrading chlorophenol; D. second screening: segregation and purifying with the solid-liquid culture medium, until the cell shape being the single thallus. The operation is simple, and the theory meaning and economic value are important. The method provides the theory basis for anaerobic biological treatment process of chlorophenol waste water. The method can be used to separate, screen and culture the chlorophenol degradation bacterium, and provide the good screening method for researching chlorophenol degradation rule and interaction between function bacteria.
Owner:TONGJI UNIV
Porous Photonic Crystal with Light Scattering Domains and Methods of Synthesis and Use Thereof
InactiveUS20080212068A1Easy to resolveImprove scattering efficiencySamplingMaterial analysis by optical meansPorous substratePhotonics
The invention includes sensors and sensing methods for determining cell morphology and / or chemical composition of an analyte. A porous substrate exhibiting a first optical signal is exposed to a target analyte and subsequently monitored for changes in the optical signal. More specifically, a photonic or porous substrate having a well-defined and highly tunable reflectivity or transmission spectrum, such as porous silicon (Si), porous alumina, porous Ge, porous GaAs, porous SiO2 and porous polymer, is used for example. A porous or photonic substrate is exposed to an analyte, such as a cell or other macromolecule, and changes in the scattered light are observed over time to determine cell morphology and / or chemical composition of the analyte using the substrate.
Owner:RGT UNIV OF CALIFORNIA
Serum-free medium used for culturing Vero cells, and preparation method thereof
InactiveCN105441378AGood proliferation rateExcellent Cell PerformanceVertebrate cellsArtificial cell constructsAntioxidantProliferation rate
The invention relates to a serum-free medium used for culturing Vero cells. The serum-free medium is composed of, by volume, 85 to 95% of a base culture solution, 2 to 5% of an amino acid solution, 2 to 7% of a serum alternative factor solution, 0.1 to 0.5% of an antioxidant solution, 0.8 to 2% of a yeast extract solution, and 0.1 to 0.5% of an ethanol amine solution. The invention also provides a preparation method of the serum-free medium. In the preparation method, the base culture solution, the amino acid solution, the serum alternative factor solution, the antioxidant solution, the yeast extract solution, and the ethanol amine solution are mixed at the above ratio so as to obtain the serum-free medium. The serum-free medium used for culturing Vero cells is capable of promoting rapid attachment of Vero cells; cell morphology of the Vero cells cultured with the serum-free medium can be maintainer preferably, and cell proliferation rate is higher; the composition of the serum-free medium is simple; cost is reduced; and preparation is convenient.
Owner:ZHAOQING DAHUANONG BIOLOGIC PHARMA
Diazonium salt modification of silk polymer
A method for modifying silk polymer by coupling a chemical moiety to a tyrosine residue of a silk polymer is described herein for the purpose of altering the physical properties of the silk protein. Thus, silk proteins with desired physical properties can be produced by the methods described herein. These methods are particularly useful when the introduction of cells to a mammal is desired, since modifications to the silk protein affect the physical properties and thus the adhesion, metabolic activity and cell morphology of the desired cells. The silk protein can be modified to produce, or modify, a structure that provides an optimal environment for the desired cells.
Owner:TRUSTEES OF TUFTS COLLEGE
Cell electrochemical sensor for analyzing joint toxicity of fungaltoxin
The invention discloses a cell electrochemical sensor for analyzing joint toxicity of fungaltoxin, belonging to the fields of analysis and detection of food quality. According to the cell electrochemical sensor, the sensitivity of the sensor is increased by virtue of electroplating gold nanoparticles, an in-vivo physiological living environment of cells is simulated by virtue of laminin, and a three-dimensional model is constructed by virtue of rat tail collagens so as to relatively truly reappear cell morphology; and the constructed cell electrochemical sensor can rapidly and sensitively respond to the influences caused by the fungaltoxin to human hepatoma carcinoma cells, equipment is convenient and rapid and low in production cost, and by combining with an electrochemical resistance value and a joint index method, the type and degree of a joint action of the fungaltoxin can be rapidly judged. The constructed portable cell electrochemical sensor is successfully applied to the toxicity analysis of the fungaltoxin in foods and has good application prospects in the analysis of the joint toxicity of the fungaltoxin.
Owner:JIANGNAN UNIV
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