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Cell three-dimensional mechanical loading unit

A technology of three-dimensional mechanics and loading device, applied in the field of cell mechanics, can solve the problem that the substrate of cells cannot be subjected to tension, and achieve the effect of improving the efficiency of tissue generation, increasing the stretching area, and being widely used

Inactive Publication Date: 2008-11-05
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the drawback of this method is that the base of the cells cannot be subjected to tension

Method used

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  • Cell three-dimensional mechanical loading unit
  • Cell three-dimensional mechanical loading unit
  • Cell three-dimensional mechanical loading unit

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0041] Example 1. Static three-dimensional cell culture of fibroblasts under the condition of no strain field (strength of 0%) and no electromagnetic field (field strength of 0 Tesla) using the cell three-dimensional mechanical loading device.

example 2

[0042] Example 2. Use the cell three-dimensional mechanical loading device to periodically stretch the fibroblasts coated with magnetic beads under the action of a magnetic field force with a field strength of 0.2 Tesla and a frequency of 100 Hz. The fibroblasts with magnetic beads were used as a control in the adjacent cell culture box to exclude the effect of the magnetic field on the cells.

example 3

[0043] Example 3. Use the cell three-dimensional mechanical loading device to periodically stretch the fibroblasts coated with magnetic beads under the action of a magnetic field force with a field strength of 0.2 Tesla and a frequency of 100 Hz, and at the same time apply 10 % strain, 1Hz circumferential periodic stretching, to study the effect of three-dimensional strain field on cells.

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Abstract

The invention discloses a drawing and loading device for cells, which relates to a cellular mechanics loading device that belongs to the medical instrument. The drawing and loading device for cells consists of a control part, a mechanical part and an electromagnetic part, which is characterized in that: the cells are cultivated on an elastic silicon membrane, and circumferential-direction drawing and compressive loading are carried out for the cells through a dynamic system, meanwhile, magnetic bead collagen with ferroferric oxide enveloped is attached to the surfaces of the cells through integrin family, then alternating magnetic field is respectively loaded at an upper and a lower bottoms of a cell cultivating box, rendering the cells to be drawn under the magnetic force while being under the circumferential stress in a base strain field, so as to control the transformation of the cells along the axis Z and control the cell morphology. By simulating the borne mechanical stress of cells under physiological conditions, the drawing and loading device for cells can realize mechanical loading with different dimensions under different mechanical forces, and has the advantages of simple structure, convenient use, wide application and excellent reproducibility, etc.

Description

technical field [0001] The invention relates to the field of cell mechanics, in particular to a cell three-dimensional mechanical loading device. Background technique [0002] The study of cell mechanics is a relatively active field. The main points of interest focus on how mechanical signals are transmitted inside cells, and the changes in the structure and function of cells under the action of mechanical signals. Mechanical stress plays an important role in physiological processes such as cell growth, differentiation, apoptosis, and gene expression, as well as certain pathological processes (such as cardiac hypertrophy, atherosclerosis, etc.). There are stress-sensitive receptors on the cell surface, and these receptors can transmit the sensed force signals to different structural components in the cell through special molecular channels on the cell surface, and realize mechanochemical conversion, thereby regulating the physiological functions of cells. [0003] Our curre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/42C12N5/00C12N13/00
CPCC12M27/00C12M25/02C12M23/20C12M35/02C12M35/04C12M23/26
Inventor 王贵学邱菊辉罗向东王玉
Owner CHONGQING UNIV
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