983 results about "Cell sheet" patented technology

An integrated scaffold for bone tissue engineering has a tubular outer shell and a spiral scaffold made of a porous sheet. The spiral scaffold is formed such that the porous sheet defines a series of spiral coils with gaps of controlled width between the coils to provide an open geometry for enhanced cell growth. The spiral scaffold resides within the bore of the shell and is integrated with the shell to fix the geometry of the spiral scaffold. Nanofibers may be deposited on the porous sheet to enhance cell penetration into the spiral scaffold. The spiral scaffold may have alternating layers of polymer and ceramic on the porous sheet that have been built up using a layer-by-layer method. The spiral scaffold may be seeded with cells by growing a cell sheet and placing the cell sheet on the porous sheet before it is rolled.

Tissue engineering devices for use in the repair or regeneration of tissue made of support scaffolds and cell sheets.

A method for identifying and isolating cells which produce secreted proteins. This method is based upon a specific characteristic or the expression level of the secreted protein by transiently capturing the secreted protein on the surface of an individual cell, allowing selection of rare cell clones from a heterogeneous population. Also provided is the use of this method to generate cells which produce a desired level of secreted protein or secreted protein of a particular characteristic(s), and organisms which possess such cells. In particular, the method allows rapid isolation of high expression recombinant antibody-producing cell lines, or may be applied directly to rapid isolation of specific hybridomas, or to the isolation of antibody-producing transgenic animals. This method is applicable for any cell which secretes protein.

Compositions, kits and methods are described that comprise one or more constructs, each construct comprising a ligand attached or conjugated to a polymer construct, e.g., an oligonucleotide sequence, by a linker, each ligand binding specifically to a single target located in or on the surface of a cell. The polymer construct comprises a) an Amplification Handle; b) a Barcode that specifically identifies a single ligand; c) an optional Unique Molecular Identifier that is positioned adjacent to the Barcode on its 5′ or 3′ end; and d) an Anchor for hybridizing to a complementary sequence, e.g., for generation of a double-stranded oligonucleotide. These compositions are used in methods, including high throughput methods, for detecting one or more targets or epitopes in a biological sample. These compositions are also used in a high throughput method for characterizing a cell by simultaneous detection of one or more epitopes located in or on the cell and its transcriptome.

The inventive method allows peptides or polypeptides to be exposed on the surface of gram-negative host bacteria using specific intimin-based anchor modules. Intimins with shortened carboxy terminals have been found to be particularly suitable anchor modules for passenger domains in the exterior E. coli cell membrane. According to the method, host bacteria are transformed using vectors, on which are located a fused nucleic acid sequence consisting of a sequence segment which codes for an intimin with a shortened carboxy terminal and a nucleic acid sequence segment which codes for the passenger peptide that is to be exposed. The invention permits a particularly large number of passenger domains to be exposed on the cell surface of the bacteria, without adversely affecting the viability of the bacteria.

A cell culture method prepares first cells that are adhesion-dependent cells and monolayered or multilayered cells on a culture surface of a culture substrate, seeds second cells that are adhesion-dependent cells and are magnetized by allowing to have magnetic particles on the first cells, induces the second cells to a predetermined position on the first cells by magnetic force, and cultures the first cells and the second cells in a cell arrangement obtained by the magnetic induction. According to this cell culture method, after a cell sheet was prepared individually, cells can be multilayered without changing a temperature, peeling the monolayered sheet and laminating the monolayered sheets.

Methods for improving the specific binding ability of bispecific binding compositions are described. The bispecific binding compositions are able to target cells by a high affinity targeting domain to a target cell surface marker and a low affinity binding domain that binds specifically to a second cell surface marker, wherein the binding of each domain to its respective cell surface marker increases or decreases, as desired, the biological activity of the respective cell surface markers. The invention further provides bispecific binding agents for use in the methods, as well as uses for the agents.

By using a bed material for cell culture having a surface composed of two domains of domain A coated with a temperature-responsive polymer and domain B composed of any one or a combination of a domain coated with a polymer having high affinity with cells, a domain coated with the temperature-responsive polymer in an amount different from the amount of the temperature-responsive polymer of domain A, and a domain coated with a polymer which responds to a temperature different from the temperature to which domain A responds, a method for the co-culture of a plurality of kinds of cells which has heretofore been difficult becomes possible.

Methods for improving the biological and pharmaceutical properties of bispecific binding agents are described herein where the bispecific binding agent are able to target cells by a high affinity binding domain to a first cell surface marker that does not induce a significant biological effect and a low affinity binding domain that binds specifically to a second cell surface marker, causing a significant and desired biological effect. Compositions of such bispecific binding agents, uses for them, and kits containing them are also provided.

The invention relates to a spread method of a polycrystalline silicon solar cell. The spread method is characterized in that the spread method comprises the following processing steps of entering a boat, warming, oxidizing, spreading, redistributing, cooling and going out the boat, wherein the spreading step comprises low temperature pre-deposition and then high temperature spreading. Reaction between a phosphorus source and a silicon wafer cannot be completed under low temperature, so that the low temperature pre-deposition is carried out on low temperature source communication at a first step of spreading, the phosphorus source cannot spread (or conduct spreading with low rate) inside a silicon wafer, the phosphorus source only accumulates on the surface of the silicon wafer, and a phosphorus film with certain thickness is formed on the surface of the silicon wafer after source communication for certain time; and the high temperature spreading is carried out on high temperature source communication at a second step, phosphorus on the surface of an original silicon wafer is reacted with the silicon wafer and spreads to the inside of the silicon wafer, and spreading rates of the center point and the periphery of the silicon wafer are same. Therefore, spreading uniformity is good, concentration distribution of impurities on the surface of the silicon wafer and inside the silicon wafer body is even, sheet resistance uniformity is improved, and final photoelectric conversion efficiency of a cell sheet is improved accordingly.

The invention relates to a solder strip of a solar module. V-shaped groove are formed on upper and lower surfaces of the solder strip, or a V-shaped groove is only formed on the soldered surface of the solder strip and a cell sheet; and the V-shaped groove is vertical to the length direction of the solder strip. The solder strip is 0.15-0.5 mm in thickness; the angle beta of the V-shaped groove is 20-75 degrees; and the vertical distance from the peak of the V-shaped groove on the upper surface to the peak of the V-shaped groove on the lower surface is 0.10-1.0 mm. The solder strip of the solar module has the following advantages that: through bending the solder strip, the internal stress of the solar cell sheet after being welded is largely reduced in the process of expanding with heat and contracting with cold so that the breaking rate of the solar cell and the hidden crack possibility of the solar module are reduced.

Mesenchymal stem cells are pluripotent cells capable of differentiating into myocardial and vascular endothelial cells. The present invention demonstrates that the mesenchymal stem cell sheet have therapeutic potential for a severely damaged heart due to its pluripotency and in situ self-renewal capability. Mesenchymal stem cells derived from adipose tissue were cultured to prepare a mesenchymal stem cell sheet. Four weeks after induction of myocardial infarction in rats, the mesenchymal stem cell sheet was transplanted to the heart. The mesenchymal stem cell sheet were readily engrafted to the surface of the scarred myocardium, grew gradually in situ, and formed a thick layer (approximately 600 μm) in 4 weeks. The grown transplanted mesenchymal tissue contained newly formed blood vessels, myocardial cells, and undifferentiated mesenchymal cells. The engrafted mesenchymal stem cells inhibited thinning of the myocardial wall in the scar area, and improved cardiac function and survival rate in rats with myocardial infarcts. Thus, mesenchymal stem cell sheet transplantation may represent a novel therapeutic approach for myocardial tissue regeneration.

The present disclosure relates to bispecific polypeptides comprising a first and a second immunoglobulin single variable domain (ISV), wherein said first ISV binds to a first target on the surface of a cancer cell with a low affinity and, when bound inhibits a function of said first target, and a said second ISV binds to a second target on the surface of said cell with a high affinity and wherein said first target is different from said second target. The present invention further discloses methods for identifying and making the same.

The invention relates to hydrogel for intelligent desorption of a cell sheet layer and an application of the hydrogel. The hydrogel is formed by dissolving functional monomers M1, chemical or physical crosslinking agents M2 and water-solubility biomacromolecules M3 in solvents 4 and adopting an oxidation reduction initiating system for free radical polymerization, and the mass percentages of M1, M2, M3 and M4 are respectively 5 to 10 percent, 2 to 10 percent, 1 to 15 percent and 75 to 92 percent. The hydrogel has excellent temperature or pH value response characteristics and biocompatibility, and the fast multiplication and desorption of cells can be effectively promoted. The cell sheet layer formed after the desorption maintains the complete cell epimatrix, and the biological function damage of the cells caused by an enzyme hydrolysis method and the cell damage caused by degradation ingredients of support frame materials are avoided. The cell sheet layer after the intelligent desorption by the hydrogel can be used for treating and repairing relevant tissues and organs of skin, corneal epithelium and parodontium ligament.

The invention discloses a cardiac muscle tonifying tablet taking an acellular biological membrane as a carrier as well as a preparation method and application of the cardiac muscle tonifying tablet. The cardiac muscle tonifying tablet taking the acellular biological membrane as the carrier is prepared by the following steps: carrying out decellularizing treatment on a natural biological membrane; then, cross-linking nutrient substances on the acellular biological membrane; planting target cells on the acellular biological membrane cross-linked with the nutrient substances; and cultivating a cell sheet which is preliminarily constructed, so that the cardiac muscle tonifying tablet taking the acellular biological membrane as the carrier is prepared. The cardiac muscle tonifying tablet disclosed by the invention is good in proliferation and differentiation activities and mechanical strength, and is sufficient in nutrition support; the cardiac muscle tonifying tablet is not only a breakthrough in tissue engineering but also suitable for the clinical treatment of myocardial infarction. The cardiac muscle tonifying tablet disclosed by the invention is reliable in principle, good in reproducibility, and suitable for standard production.

The invention discloses a capture sieve and a device for capturing cells or biomolecules in solutions. The capture sieve comprises a net-shaped base body and a capture layer formed on the net-shaped base body, wherein the capture layer comprises capture matter which can be in specific binding with target cells or biomolecules. The capture sieve and the device provided by the invention have high specificity and high flux, are suitable for capturing the cells or capturing the biomolecules in the solutions by adopting the molecules expressed by the cells, and are particularly suitable for capturing and sorting circulating tumor cells.

The invention discloses a packaging adhesive film for a solar cell module. The packaging adhesive film for the solar cell module comprises a blocking layer, a first adhesive layer and a second adhesive layer, wherein the first adhesive layer and the second adhesive layer are located at the two sides of the blocking layer, the blocking layer is a polyolefin blocking layer containing ethylene-a-olefin polymer. The adhesive film is good in blocking performance and capable of effectively avoiding the PID phenomenon of the solar cell module under the high-pressure, damp and hot environment, the adhering performance between the packaging adhesive film and a glass substrate and between the packaging adhesive film and a cell sheet is good, and the service life of the solar cell module is prolonged.

The present invention provides a polypeptide having the formula: St-R1-S1-Q-S2-R2 wherein St is a stalk sequence which, when the polypeptide is expressed at the surface of a target cell, causes the R and Q epitopes to be projected from the cell surface; R1 and R2 are a Rituximab-binding epitopes each having the an amino acid sequence selected from the group consisting of SEQ ID No. 1, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 and 16 or a variant thereof which retains Rituximab-binding activity; S1 and S2 are optional spacer sequences, which may be the same or different; and Q is a QBEnd1O-binding epitope having the amino acid sequence shown as SEQ ID No. 2 or a variant thereof which QBEnd1O-binding activity. The invention also provides a nucleic acid sequence encoding such a polypeptide and uses thereof in adoptive cell transfer.

A carrier for cell culture comprising an alginic acid gel layer laminated with a gel layer containing a cell adhesion substance, wherein the gel layer containing a cell adhesion substance has a dry thickness of less than 0.3 mum. The carrier for cell culture enables reproducible and convenient cell layer lamination without inhibiting growth and proliferation of cells upon solubilization of the carrier for preparation of a cell sheet.

An anterior ocular segment related cell sheet or three-dimensional structure that have only a few structural defects as they have been recovered retaining the intercellular desmosome structure and the basement membrane-like protein between cell and substrate. The anterior ocular segment related cell sheet or three-dimensional structure is produced by a process comprising the steps of cultivating cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer having an upper or lower critical dissolution temperature of 0-80° C. with respect to water, optionally stratifying the layer of cultured cells by the usual method, and thereafter, (1) adjusting the temperature of the culture solution to either above the upper critical dissolution temperature or below the lower critical dissolution temperature, and further optionally (2) bringing the cultured anterior ocular segment related cell sheet or three-dimensional structure into close contact with a polymer membrane, and (3) detaching the sheet or three-dimensional structure together with the polymer membrane.