59 results about "Metaphase" patented technology

Methods and apparatus are provided for the automated analysis of images of living cells acquired by time-lapse microscopy. The new methods and apparatus can be used for the segmentation, classification and tracking of individual cells in a cell population, and for the extraction of biologically significant features from the cell images. Based upon certain extracted features, the inventive image analysis methods can characterize a cell as mitotic or interphase and / or can classify a cell into one of the following mitotic phases: prophase, metaphase, arrested metaphase, and anaphase with high accuracy.

A method of cellular evaluation based on the electronic nature of cells is reveled though cellular reproductions use of a magnetic force. The dynamic process of nuclear response is shown to be electronic in nature relative to DNA mediating electrons hydrogen bonding in bases pairing of DNA though out the a cell cycle and finally during metaphase one see the magnetic component of interaction. The electrostatic understanding of magnetic force is not well defined in physics in the process of electrodynamic. Cells use electrodynamic interaction within the cell are being studied as the basis and using the cell to measure and define electrodynamic interaction with the system that is biological a call. Specifically DNA thought the electronic interaction interactions. It appears infrared spectrum holds promises to help in revel these mechanisms. The promise of understanding or merely evaluation of electrodynamic interaction holds great promise to science with the greatest medical implication to understand genomic responses in cells. Understanding how the DNA interacts within a cell dynamic transition are known to take place and these are regulated thought electrodynamic interaction.

The method for increasing commodity rate of breeding black shrimps in the invention includes selecting excellent seeds, postponing the time of shrimp postlarvae stocking properly and feeding some chubs, bighead carps, etc., controlling the coverage rate of float grass and the transparency of water, and making use of calces. When in the metaphase of breeding black shrimps, more particularly, when the black shrimps are in the propagation season of Autumn, the area of float grass is controlled to be 60-70% of the water area, the transparency is controlled to be 40-50cm. In the propagation season, when the color of oogenesis of shrimps becomes grey and transparent, or flea-shape larva is hatched out, calces of 10-20g per stere is used for spraying the whole pool within five days, then the method is used every week and is totally used for 7-10 times. By increasing the measures of controlling the coverage rate of float grass and the transparency of water and making use of calces, neoteny of black shrimps and over propagation are controlled, the growth of grown shrimps is promoted, and the commodity rate of black shrimps can reach 90% at the end of the year. The method of breeding black shrimps has the advantages of easy and convenient operation as well as easy popularization and use, etc., which is a good method for effectively increasing commodity rate of breeding black shrimps.

The invention provides an excised mutagenesis tetraploid method of water melon and ploidy early stage certification technique, wherein dinitro toluene herbicide (DNH) is employed to substitute the conventional colchicines as inducer, whose function is to suppress the Mitosis in the metaphase of cell division through the mechanism of interfering spindle, so as to double the tissue cell chromosome. The method has the advantages of increased inducement success rate and substantially shortened time required for inducement.

The invention provides a multicolor fluorescence in situ hybridization (MFISH) method for quickly analyzing and identifying alien chromosome of wheat. The method comprises the following steps: (1) preprocessing actively dividing tissues in meristematic zone of wheat root tip by N2O and conducting enzymolysis on the actively dividing tissues to obtain a metaphase specimen with clear images and uniform distribution of chromosomes; (2) marking a fluorescent probe of fundamental genome of wheat by nick translation method; (3) conducting fluorescence in situ hybridization on chromosomes at mitosis metaphase obtained in step (1) by using the constructed fluorescent probe in step (2); and (4) analyzing the fluorescence in situ hybridization results obtained in step (3). According to the method provided by the invention, not only can alien chromosomes and chromosome segments in distant hybrid wheat be identified, but also alien chromosomes and chromosome segments of distant hybrids of other crops can be identified.

The invention discloses a method for extracting triterpene substances from inonotus obliquus by use of quorum sensing molecules induction, wherein the method comprises the following steps: adding activated inonotus obliquus CFCC 83414 into a liquid medium, and performing fermentation culture for 8-14 days at 24-26 DEG C; adding quorum sensing molecules in the 5th-8th days of the fermentation; after the fermentation, separating thalli and fermentation liquid; performing aftertreatment to separate and purify triterpene substances from the thalli and fermentation liquid. According to the method disclosed by the invention, quorum sensing molecules are added in the fermentation metaphase to stimulate growth of inonotus obliquus; the method does not need introduction of a gene engineering strainor additional enzyme, is simple in aftertreatment, and can effectively increase the yield of triterpene substances in inonotus obliquus.

The invention discloses a breeding method for a sow in a reproduction period. The method comprises the following steps: feeding in a reserve period, feeding in a pregnancy period, and feeding in a lactation period. According to the invention, directed at actual states of the sow in all stages of the reproduction period, targeted feeding with a feed with different components and proportions is economic, scientific and reasonable, and fully meets the requirements of the sow for nutrients in all stages at the same time, so the sow can remain a good growth state from oestrum to piglet delactation, i.e., the sow in an oestrous period has the advantages of well-developed, high-quality and numerous ova, high pregnancy rate, high survival rate of fertilized ova, high survival rate of early embryos in the early stage of mating, good developmental state of metaphase embryos, and high survival rates of the piglets after birth and in the lactation period; thus, the marketing rate of commercial pigs is fundamentally increased, and pig-raising efficiency is improved.

The invention relates to a biological agent and particularly relates to a lymphocyte culture medium. The lymphocyte culture medium comprises lectin, a basal culture medium and a serum substitution, wherein the lectin is L-type phytohemagglutinin; the basal culture medium is an RPMI1640 basal culture medium; and the serum substitution comprises bovine serum albumin, recombinant human insulin, ferric citrate and an amino acid mother solution. By adopting the serum-free human peripheral blood lymphocyte culture medium, the animal or human serum is not used so that the cost of the culture medium is reduced and the disease dissemination risk is also reduced. The components of the serum-free human peripheral blood lymphocyte culture medium are determined so that the problems of complex traditional serum components, quality differences in different batches and fluctuated culture effect can be avoided. The culture medium provided by the invention is high in lymphocyte conversion rate and lymphocyte metaphase index, low in reagent cost and stable in quality, and the cultured cell can be used for clinical diagnosis of the karyotype analysis.

The invention discloses a fertilization method under the condition of drip irrigation cultivation under corn film, which belongs to the field of agricultural planting technology. The method is: mechanical fertilization, the fertilizer applicator is set with three fertilization ports, three rows of fertilizer are applied on each large ridge, and the middle row of fertilizer is applied to the ridge In the middle, the distance between the fertilization openings on the outermost two sides is 50-55cm; apply 60% fertilizer in the middle, and 20% fertilizer on each side; apply 15-17cm deep in the middle, and 8-10cm deep on both sides place. The beneficial effects are as follows: 1. Cross-layer fertilization. In the early stage of corn growth, its root system is short and can absorb shallow fertilizer, while in the middle and late stages of corn growth, its root system is longer and can absorb deep fertilizer; 2. Fertilize on both sides. There are fertilizers on both sides of the corn plant, and the closest distance to the fertilization belt is 5cm, which fully exerts the absorption of the fertilizer by the whole plant and improves the utilization rate of the fertilizer; 3. Fertilize on demand. Corn needs more fertilizer at the jointing and booting stage. At this time, the root system is longer and can absorb deep-level fertilizers, and the deep-level fertilizers account for a large proportion, which can just meet the needs of corn for fertilizers.

The invention discloses a fluorescence in situ hybridization (FISH) method for fish chromosomes. In the method, on the basis that a metaphase split phase specimen with a clear image and good chromosome spreading is obtained, a probe is marked by FISH technology and a nick translation method by taking Biotin-16-dUTP as a marker, a hybridization signal is amplified at two stages and a human 5.8S+28SrDNA probe is clearly positioned on a polyploid fish chromosome nucleolus organizer region (NOR). The chromosome ploidy and a karyotype of a polyploid are compared and analyzed by observing the chromosomal localization of ribosome 5.8S+28SrDNA on a polyploid fish, namely, the polyploid fish is proved to be a genetic polyploidy or an evolution polyploidy and an autopolyploid or an allopolyploid byanalyzing.

The invention relates to a chromosome karyotype analysis system which comprises the following steps: (1) designing a filtering algorithm and a segmentation algorithm to filter and remove impurities from a human metaphase cell image, and extracting a staining monomer; and (2) designing an identification algorithm and a correction algorithm, and identifying and pairing the extracted chromosomes so as to generate a karyotype map. According to the invention, the karyotype analysis method is combined with image processing, machine learning and other technologies, a reliable chromosome karyotype automatic analysis system is developed, automation and intelligence of chromosome karyotype analysis are realized, and the efficiency and the accuracy of chromosome karyotype classification are integrally improved.

A duct stent being capable of carrying the subminiature radioactive particle source is mainly used for the inside radiation therapy of metaphase or terminal pancreatic cancer and bile duct cancer, and has drainage function. The duct stent consists of a tubular drainage tube (1) and fixation portions (2) which are face to face with two ends of the drainage tube (1) and can fix the stent to prevent the stent from moving. The lumen of the drainage tube (1) is a drainage cavity (1.1) for draining the pancreatic juice or the bile. A particle passage (1.2) is provided in the tube wall of the drainage tube (1). The inside diameter of the particle passage (1.2) matches with the outer diameter of the subminiature radioactive particle source.

The present invention provides methods of producing a cloned non-human mammalian nuclear transfer (NT) embryo and methods for producing a cloned non-human mammal. Embodiments of the methods include introducing donor genetic material into a metaphase I oocyte; introducing donor genetic material into a non-enucleated oocyte; introducing donor genetic material obtained from a donor cell that is at metaphase into an oocyte; introducing donor genetic material into an oocyte, and naturally activating the oocyte or the NT embryo; and introducing donor genetic material obtained from a donor cell that is at late G1 phase into an oocyte.

The present invention relates to a method for cell enucleation, comprising removal of the spindle body from a cell in metaphase with a minimal amount of cytoplasm from the cell, said method comprising using polarized light microscopy for visualization of the spindle body during cell enucleation. The present invention also relates to a method for production of an activated reconstructed vertebrate cell, said method comprising: a) culturing a reconstructed vertebrate cell for about 1.5 to about 3 hours after being reconstructed; b) applying at least one electrical pulse to the reconstructed cell; c) culturing the reconstructed cell for about a further 2 hours; and d) treating the reconstructed cell with a chemical activator. The methods of the invention find application in the preparation of totipotent or pluripotent cells of substantially identical genotype, as well as the cloning of vertebrates.

The invention discloses corneal metaphase preserving liquid containing recombinant human serum albumin and a preparation method thereof. The corneal metaphase preserving liquid containing recombinanthuman serum albumin contains glutamine, calcium pantothenate, choline chloride, folic acid, inositol, nicotinamide, pyridoxal.HCl, riboflavin, thiamine.HCl, KCl, NaCl, NaH2PO4.2H2O, glucose, dextran 40, sodium pyruvate, vitamin C, chondroitin sulphate sodium, sodium hyaluronate, non-animal recombinant human serum albumin, gentamicin sulfate, sodium bicarbonate, piperazine-1-erhanesulfonic acid, phenol red indicator, and water for injection. The non-animal recombinant human serum albumin is added to replace serum to improve corneal endothelial cell survival rate; and then the non-animal recombinant human serum albumin cooperates with a compound system of the chondroitin sulphate sodium, the sodium hyaluronate and the dextran 40, the survival rate of the corneal endothelial cell is greatly ensured.

The invention discloses a method for identifying all chromosomes of poplar by using oligonucleotide probes. The method comprises the following steps: obtaining a specific oligonucleotide sequence of each chromosome through bioinformatics analysis according to genome information of poplar tomentosa; synthesizing an oligonucleotide library corresponding to chromosome 1-19 of poplar; marking the oligonucleotide probes by using biotin or digoxin; identifying the chromosomes of the poplar by using a fluorescence in-situ hybridization technology; and repeatedly carrying out fluorescence in-situ hybridization for 7 times on the same metaphase splitting phase by using the oligonucleotide probes of up to three chromosomes simultaneously in each cycle of hybridization, so that 19 pairs of chromosomes of poplar species can be accurately identified.The method has the advantages of good universality in poplar species, good experimental repeatability and high resolution.The methoddisclosed by the invention provides a novel method for accurately identifying each chromosome of the poplar species, and lays a solid working foundation for molecular cytogenetics research of poplar.

The invention discloses a medicament for rapidly treating burns and scalds. The finished product of the medicament is mainly prepared by fully mixing three components, namely ectotherm oil and fat, a centipede soaking solution and a beewax soaking solution, and the medicament belongs to the technical field of traditional Chinese medicines. Further, the prepared finished product is placed under a low-temperature condition of 4DEG C and is stood for 24 hours; then supernate is separated to obtain two types of products, namely paste products and liquid products. The medicament disclosed by the invention has the efficacy of resisting bacteria, diminishing inflammation, relieving swelling and pain, clearing away heat and toxic materials, removing necrotic tissue and promoting fresh blood production, drawing out toxin and promoting tissue regeneration, and the like, can be used for rapidly treating scales and burns caused by various reasons, does not need a desinfection chamber, can be applied any time and any place, and is suitable for popularization in vast rural areas and onsite rescue; according to the medicament, severe pains at an initial stage brought by the burns and scalds can be rapidly relieved, and infection under scabs in a metaphase is effectively prevented; the medicament is short in treatment cycle, good in effect, high in cure rate and free of scars after cure.

The invention provides an efficient sugarcane or sugarcane related species stem tip chromosome flaking method, and belongs to the technical field of cell biology. The invention provides an efficient sugarcane stem tip chromosome flaking method aiming at solving the problems that sugarcane chromosomes are large in number and small in form and ideal division phase metaphase cells are difficult to obtain through root tip flaking. Chromosome flaking is carried out by utilizing stem tip meristematic region tissues of sugarcane and sugarcane related species in a vigorous growth period, the method has the advantages of convenient material taking, large meristematic area tissue sample size, vigorous division, many metaphase cells and clear and dispersed chromosome structure, and the technical keypoints of material selection, pretreatment, fixation, dissociation dyeing, flaking and chromosome morphology microscopic observation are disclosed. The method is simple and convenient, accurate and reliable in result, good in repeatability, easy to operate and short in experiment period and improves the sugarcane chromosome genome analysis efficiency. Technical support is provided for sugarcane chromosome karyotype research, germplasm resource classification identification and protection utilization.

The invention relates to a preparation method of a turbellarian worm chromosome specimen. The preparation method of the turbellarian worm chromosome specimen comprises the steps of material preparation, pretreatment, regenerated tissue culture, tissue smashing, centrifugal suspending, colchicine blockage, KCl low permeability treatment, centrifugal suspending and dripping, dyeing, baking and sheet sealing. By adopting the preparation method, regenerated tissues and metaphase cells are fixed and treated fully, the chromosome preparation background is clear, cell dispersion is high, good cell scattering is realized, the number of metakinesis phases is large, and karyotype analysis is facilitated.

The invention relates to a preparation detection method of a biofilm. A stable microorganism biofilm can be formed on a carrier through the steps of ultrasonic carrier cleaning, microbiological culture, carrier biofilm formation, biofilm detection and the like. The specific surface area of the carrier can be increased by ultrasonically cleaning the carrier, so that the apposition growth of more microorganisms can be carried out on the biofilm, and the biomass of the microorganisms is increased. Biofilm formation is started from the metaphase of the logarithmic growth phase of the microorganisms, so that the time of biofilm formation can be shortened, and the efficiency of biofilm formation can be effectively increased. The growth condition of thalli on the biofilm can be accurately monitored in time by using the blood corpuscle counting mode. The preparation detection method of the biofilm is quick in screening and can be widely applied to the fields of environment water treatment, biomedicine materials and the like.

The invention discloses a cell telescoping structure model induced through mitosis retarding and a preparation method thereof. The method includes: retarding the mitosis metaphase through chemical drug or a genetics method to obtain a CIC cell model. The CIC cell model is induced through targeted retarding of mitosis. Building of the cell model is not limited in treatment mode and can be realizedno matter through the chemical drug or through the genetics method, and a stable and reliable model is provided for studying formation mechanism and physiological and pathological significance of CIC.Mitosis retarding induced CIC structure can be realized in normal epithelial cells, and the CIC cell model can be built in tumor cells. The CIC cell model can be built by using tumor targeting drug for targeted retarding of the mitosis metaphase, it shows that the model can serve as an in-vitro model for tumor therapy research.

The invention discloses a comprehensive control method for a wheat cyst nematode. The method includes the following steps: a, a wheat breed of Wenmai 4 or Taikong 6 that resists diseases is adopted; b, a nematocide ethoprophos is adopted during a seeding period and the processing method is to broadcast the ethoprophos with a dosage of 15 to 45Kg / hm<2> before plowing during a wheat seeding period and then turn over thereof into the soil; c, late broadcasting is adopted and the broadcasting period can be late according to the actual situation of the area for the early broadcasted wheat has a heavier disease; d, timely irrigating: the seedling stage, the overwintering as well as the reviving and jointing stage of wheat are three key stages which can be irrigated in time during the prophase and the early time of the metaphase of wheat growing. As adopting the method and steps, the invention has strong pertinence and good effect. Due to field experiments, the invention has no harm on wheat growing and the comprehensive control measure can ensure the control effect of wheat cyst nematode to reach about 90 percent.

The invention belongs to the field of molecular cytogenetics and particularly relates to a method for identifying D genome and D sub-genome complete chromosome. The method comprises the following step: by taking a sequence as shown in SEQ ID NO.1 as a probe to perform fluorescence in-situ hybridization on the DNA (deoxyribonucleic acid) of a cotton genome. The sequence is taken as the probe, and chromosomes in the mitosis metaphase of the upland cotton, the asiatic cotton and the Raymond's cotton are taken as target DNA to perform fluorescence in-situ hybridization, and the results indicate that obvious hybrid signals are shown on the D subgenome chromosome of the upland cotton and the D genome chromosome of the Raymond's cotton, the signals are distributed on all chromosomes, and no obvious signals are shown in the A subgenome chromosome of the upland cotton and the A genome chromosome of the asiatic cotton. The sequence adopted by the method disclosed by the invention can be used for quickly identifying the cotton D genome and D sub-genome complete chromosome by virtue of a FISH (fluorescence in situ hybridization) method.

The invention provides a preparation method of feeding lactic acid bacteria micro pills. The preparation method comprises the following steps: culturing lactic acid bacteria until the metaphase of a logarithmic growth phase appears; adding sodium chloride into a culture solution to perform continuous culture until the prophase of a stable growth phase appears; centrifuging; cleaning bacterial sludge by using normal saline, and collecting the bacterial sludge; re-suspending the bacterial sludge in a fresh MRS culture medium, slowly heating to 45-55 DEG C, keeping the temperature for 30 minutes, then quickly cooling to 0-4 DEG C, taking out the bacterial sludge, and uniformly mixing the bacterial sludge with an appropriate amount of a filling agent, sodium alginate, lactalbumin, xylooligosaccharide, fructo-oligosaccharide, resistant starch and an antioxidant to obtain a mixture; and then putting the mixture into an extrusion pill-rolling machine for pelletizing, and drying after pelletizing to obtain the feeding lactic acid bacteria micro pills. The preparation method can be used for effectively improving the stability and tolerance of the lactic acid bacteria in the micro pills, so that the survival rate of the lactic acid bacteria during storage, transportation and processing of the lactic acid bacteria micro pills is increased and the living bacterium rate of a feed is increased; and meanwhile, the preparation method is simple and easy to control, and is suitable for industrial large-scale production.

The invention belongs to the technical field of plant identification and discloses a tabletting method of eucalyptus chromosome.The tabletting method includes following steps: taking materials, pretreating, fixing, dissociating, dyeing, tabletting and performing microscopic examination.In the tabletting method, oryzalin is adopted as a pretreatment agent, so that cytotoxic effect is reduced obviously when cell mitosis is blocked; cells can be in normal mitosis, so that during fixing, number of accumulated metaphase cells is large, chromosome tabletting success probability is increased, and stability is improved.Oryzalin is low in cost and far lower than colchicine in toxicity; in the tabletting process, environment pollution can be reduced and degree and risk of causing damage to people are lowered.

The invention discloses a method for improving the mitotic phase of a chrysanthemum root tip by artificial regulation. With the artificial regulation method, chrysanthemum tissue-cultured seedlings are processed under the experiment-verified conditions of the proper dark processing and illumination (strong) time, so that the clean chromosome film is obtained and the metacinesis numbers of the mitosis metaphase of the cell can be increased effectively (shown in a figure I and a figure II). The utilized raw materials are available; the method is simple and is easy to implement; the cost is low;and the operability and practicability are high. On the basis of dark processing and illumination processing, the mitosis metaphase phase number is increased; and a clean root-tip mitotic metaphase chromosome image is obtained and the obtained image can be applied to identification of the chrysanthemum mitosis chromosome. Besides, the cytological study for karyotype analysis and FISH analysis andthe like can be realized. The method for acquiring the clear root-tip mitotic metaphase chromosome image of the chrysanthemum can be promoted to the cytological study of other chrysanthemum plants.

The invention discloses a method for preparing a pelochelys bibroni chromosome specimen by using peripheral blood cells, and belongs to the field of cytogenetics. Peripheral blood of a pelochelys bibroni is collected, and a pelochelys bibroni chromosome metaphase specimen which is convenient to observe and clear in image is prepared through the steps of blood cell culture, colchicine treatment, staining and slide preparation and the like. According to the method of the invention, the problem of large damage to animals in an existing aquatic animal chromosome specimen preparation method is solved, non-damage sampling is realized, only a small amount of peripheral blood of the sample needs to be collected, and the influence on the living state of the animals is small. The slide preparation method is simple and rapid; the metaphase of the pelochelys bibroni chromosome can be obtained 72 hours after blood sampling, observation is facilitated, and a beneficial tool is provided for further developing pelochelys bibroni genome and evolution related research and realizing more sufficient protection of the pelochelys bibroni .

The invention relates to a method for preparing a planarian chromosome sample. The preparation method of the planarian chromosome specimen of the present invention comprises the following steps: material collection, pretreatment, regeneration tissue culture, tissue crushing, centrifugal suspension, colchicine blocking, KC1 hypotonic treatment, centrifugal suspension dripping, dyeing and drying and sealing piece. The preparation method of the invention fully fixes the regenerated tissue and the metaphase cells, has a clear chromosome preparation background, well-dispersed cells, and many metaphase division phases, which is beneficial to karyotype analysis.

We have developed a chicken (Gallus domesticus) Z-chromosome-specific DNA library in a phage vector, by means of chromosome microisolation and microcloning. The chromosomal origin, specificity and purity was evaluated by fluorescent in situ hybridization (FISH) on chicken metaphases. Heterologous chromosome painting, using this Z-chromosome-specific probe on turkey (Meleagris gallopavo) metaphases identified its homologous Z-chromosome, under the same stringent conditions as that used in the chicken, indicating a high degree of Z-chromosome sequence homology among these two species. This chicken Z-chromosome library will facilitate the development of Z-chromosome-specific DNA markers that will be useful for genetic mapping in the domestic chicken and related avian species. The Z-chromosome-specific DNA probe will also be useful for studies pertaining to the sex chromosome evolution in avian species.