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744 results about "Microscopic exam" patented technology

Microscopic examination in a biochemical laboratory. Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye) .

Method and apparatus for automated reprocessing of tissue samples

A method and apparatus of automatically reprocessing a specimen for microscopic examination is disclosed. Processing of a specimen for microscopic examination involves fixation of the specimen and preparation of the embedded specimen from the fixed specimen. There are instances where, once a specimen has been processed, it is necessary to reprocess the specimen due to contamination of reagents during processing or inadequate fixation. The system automatically reprocesses a specimen by removing residual embedding material from the specimen with a clearing agent, removing the clearing agent with a dehydrating agent, and removing the dehydrating agent with an aqueous fluid.
Owner:VENTANA MEDICAL SYST INC

Method for producing inocula for livestock and poultry by multi-thalli mixed liquid

The invention relates to a method for fermentation production of a poultry bacterial agent by multi-bacteria miscible liquid, wherein aerobic bacteria, namely Bacillus subtilis, Bacillus licheniformis, bacillus natto, beer yeast, Aspergillus niger and Aspergillus oryzae are cultured in a shaking table according to different culture mediums, so as to culture a mother seed solution; simultaneously Lactobacillus acidophilus, bifidobacteria and enterococcus faecalis are subjected to anaerobic culture by utilization of Kille flasks, and a mother bacterial solution of photosynthetic bacteria is cultured by a Kille flask under the condition of illumination; the prior stock solution is inoculated into a seed tank for anaerobic culture and fermentation according to 4 percent of the inoculum concentration, and the fermentation time is between 48 and 60 hours; and the cultured seed liquid is inoculated into a productive tank for anaerobic culture and fermentation according to 10 percent of the inoculum concentration, the fermentation time is between 60 and 72 hours, and the fermentation end point is reached when the pH value is reduced to 4.0. The method has the advantages that the microscopic examination viable count of the bacterial agent is more than 5 billion per milliliter, so that the bacterial agent is safe and nontoxic, thereby not only improving the disease resistance of poultry but also promoting the quick growth of the poultry, reducing the feed-meat ratio and improving the quality of meat, eggs and milk.
Owner:张培举

Hatching device and method for demersal spawns of marine fishes

The invention relates to a hatching device and method for demersal spawns of marine fishes. The hatching device comprises a net cage, a water channel, a bracket, a temperature controller indicator, a probe, a heating rod, a water inlet hose, a sinker, an air inlet hose, an air stone and an annular water injector, wherein the net cage and the bracket are located in the water channel; the net cage is bound on the bracket; the water inlet hose and the air inlet hose are located in the water channel outside the net cage; the upside of the net cage is provided with the annular water injector; and the probe and the heating rod are arranged in the water channel. The hatching method comprises the following steps of: controlling a hatching condition; hatching; carrying out microscopic examination observation; carrying out secondary water temperature controlling; managing routinely; collecting larva fish and the like. The hatching method has the advantages that spawn deposition is avoided because of conical point inflation; spawns adhered on net cage edges are washed at any time by the annular water injector; the net cage is conveniently replaced; the water temperature is stably controlled by an electric temperature controller; and the fresh water quality can be ensured due to water feeding and drainage modes of water feeding of the annular water injector at the upside and water feeding of the water channel at the bottom and overflowing of the periphery of the water channel. Therefore, by means of the hatching device and method, a hatching rate of the demersal spawns of the marine fishes in the industrial fry breeding is improved.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Method for counting and characterizing aggressive diamonds in cmp diamond conditioner discs

The present invention is a method for determining the location of and distinguishing aggressive diamonds from active diamonds on a diamond conditioner disc, comprising: (a) contacting a diamond conditioner disc with a hard surface, wherein the diamond-containing side of the diamond conditioning disc is facing the hard surface, (b) pushing the conditioner disc a sufficient distance that all diamonds could possibly be scratching the surface at the same time and at least a distance corresponding to the length of the said diamond conditioner disc (c) observing number and position of the scratches left by diamonds on the hard surface to determine the number and position of active diamonds on the diamond conditioner disc, and (d) selecting the diamonds, the marks for which are the most pronounced and which comprise 50% or more of the total furrow area observed for all of the active diamonds in descending order of furrow are plus any diamonds in excess of the number needed to achieve said 50% or more whose individual furrow area is 2% or more, which diamonds are determined to be aggressive diamonds, or impressing the diamond conditioner disc under a load onto a hard surface and the impression of the most aggressive diamonds in the hard surface being confirmed by microscopic examination to in turn confirm the position and aggressiveness of the aggressive diamonds observed or (e) contacting a diamond conditioner disc with a hard surface, wherein the diamond-containing side of the diamond conditioning disc is facing the hard surface, (f) pushing the conditioner disc a sufficient distance that all diamonds could possibly be scratching the surface at the same time and at least a distance corresponding to the length of the said diamond conditioner disc (g) observing number and position of the scratches left by diamonds on the hard surface to determine the number and position of active diamonds on the diamond conditioner disc, (h) the hard surface further comprises a layer of contrasting material such that when the diamond conditioner disc moves across the hard surface, the said diamond conditioner disc crosses the limits of the layer entirely from one end to the other and scratches the layer of contrasting material on the hard surface thereby leaving a visible mark, (i) the said layer is between 8 and 15 microns thick and (j) selecting the diamonds which cut entirely through the said layer allowing backlighting to be easily viewed.
Owner:ARACA

Method for detecting density distribution of micro-plastics in soft tissues of marine organisms

The invention discloses a method for detecting density distribution of micro-plastics in soft tissues of marine organisms, and belongs to the field of micro-plastics in fish or bivalve organism samples. The method for detecting the density distribution of the micro-plastics in the soft tissues of the marine organisms comprises the following steps of selecting the fish or bivalve organisms with similar individuals, washing the fish or bivalve organisms with pure water to prepare digestive tract or soft tissue samples, and recording the weights; preparing a digestion solution for digesting the prepared samples, and obtaining a mixed digestion solution; adding NaCl into the mixed solution to carry out density flotation; and carrying out microscopic examination on particles subjected to flotation, selecting the suspected particles to be subjected to analysis of a microscopic-Fourier spectrometer and the like, performing counting after the particles are identified as the micro-plastics, andperforming calculation to obtain the density distribution of the micro-plastics. According to the method, the micro-plastics in the digestive tracts or the soft tissues of the marine organisms can beaccurately quantified, thereby providing basic data for the pollution condition of the micro-plastics in an area.
Owner:ZHEJIANG UNIV +1

Screening method for producing chondroitin sulfate bacterial strain and application of bacterial strain fermentation method in production of chondroitin sulfate

The invention relates to a screening method for producing chondroitin sulfate bacillus subtilis and application of a bacterial strain fermentation method in the production of chondroitin sulfate, belonging to the technical field of biological engineering. The bacterial strain disclosed by the invention is bacillus subtilis which is obtained by the following steps of: diluting and coating fermented soya beans subjected to a boiling water bath, dyeing and carrying out microscopic examination on an obtained soporiferous strain, then carrying out primary fermentation and shaking culture one by one on screened bacterial strains, adding chondroitin sulfate standard substance of sharks in a formation liquor to be used as an internal standard, carrying out qualitative analysis and screening by adopting a high performance liquid chromatography, and carrying out morphological, physiological and biochemistric and molecular biological identification on the screened bacterial strains to obtain thebacillus subtilis for producing the chondroitin sulfate. Meanwhile, the invention discloses a method for producing the chondroitin sulfate by using the bacterial strain; and the method is used for fermenting the chondroitin sulfate for 24h, wherein the yield of the chondroitin sulfate is 177mg / L.
Owner:HUNAN WUXING BIOLOGICAL TECH CO LTD

Bacillus vallismortis and application thereof

The present invention provides Bacillus vallismortis SZ-4, which has the preservation number of CGMCC No.8273. According to the present invention, the strain grows on a beef extract-peptone (NB) culture medium in a single cell reproduction growth manner to form bacterial colonies, and the bacterial colonies have characteristics of round shape, milk white color, slightly elevated center, and moist and transparent surface; microscopic examination results show that the Bacillus vallismortis SZ-4 has a rod shape, has flagella, has a size of 0.7-0.82*1.6-2.2 mum, presents the positive Gram staining effect, has spores, and can grow on a beef extract-peptone (NB) culture medium containing 2-5% of NaCl, wherein the beef extract-peptone (NB) culture medium comprises: 3.0 g of a beef extract, 10.0 g of peptone, 5.0 g of NaCl, 17 g of agar and 1000 ml of water, and the pH value is 6.8-7.2; and the Bacillus vallismortis SZ-4 provides significant antagonism effects for pathogenic bacteria causing ginseng root rot, blight, sclerotinia sclerotiorum and botrytis cinerea. The present invention further provides an application of the Bacillus vallismortis SZ-4 in prevention and control of plant fungal diseases or preparation of microorganism preparations for prevention and control of plant fungal diseases.
Owner:JILIN AGRICULTURAL UNIV

Quantitative fecal examination apparatus

A quantitative fecal examination apparatus includes a fecal collector removably mounted on a container base, having the fecal collector provided with a suction portion for sucking a fecal specimen into the collector and then the fecal specimen is disposed into the container base for examination test or for sucking the fecal specimen for further laboratory test including microscopic inspection, and having a quantitative design in the examination apparatus for collecting the fecal sample in a pre-set quantity for more precise examination.
Owner:CHANG MAO KUEI
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