749 results about "Microscopic exam" patented technology

A microflow device for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts which are arranged across the width of a collection region in an irregular or set random pattern so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic permeable hydrogel coating. The collection region is formed as a cavity in a body molded from PDMS, which flexible body is sandwiched between a glass slide or comparable flat plate and a rigid top cap plate, both of which are pressed into abutting relation with the PDMS body by a heat-shrunk polymeric sleeve. Following cell separation and washing, cells can be released from the sequestering agents and the device centrifuged to force said cells to collect adjacent the hydrogel-coated slide or plate. Slitting the polymeric sleeve allows the body to then be peeled from the slide or plate, using an integral tab, to expose the separated cells on the top surface thereof for ready microscopic examination.

An intravaginal fertilization and culture container includes a main chamber and a microchamber. A culture medium, one or more oocytes and sperm are introduced through an access opening having an associated resealable closure device. The container is enveloped in a capsule of soft elastic material prior to lodgment in the posterior fornix. A loop on one capsule part adapts the length to anatomic variations. The microchamber has a channel which receives the catheter tip to facilitate embryo retrieval while eliminating risk of injury thereto. The microchamber permits embryos to be microscopically inspected in situ before transfer to the uterine cavity.

The technology disclosed relates to scanning of large flat substrates for reading and writing images. Examples are flat panel displays, PCB's and photovoltaic panels. Reading and writing is to be understood in a broad sense: reading may mean microscopy, inspection, metrology, spectroscopy, interferometry, scatterometry, etc. of a large workpiece, and writing may mean exposing a photoresist, annealing by optical heating, ablating, or creating any other change to the surface by an optical beam. In particular, we disclose a technology that uses a rotating or swinging arm that describes an arc across a workpiece as it scans, instead of following a traditional straight-line motion.

A microflow device for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts which are arranged across the width of a collection region in an irregular or set random pattern so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic permeable hydrogel coating. The collection region is formed as a cavity in a body molded from PDMS, which flexible body is sandwiched between a glass slide or comparable flat plate and a rigid top cap plate, both of which are pressed into abutting relation with the PDMS body by a heat-shrunk polymeric sleeve. Following cell separation and washing, cells can be released from the sequestering agents and the device centrifuged to force said cells to collect adjacent the hydrogel-coated slide or plate. Slitting the polymeric sleeve allows the body to then be peeled from the slide or plate, using an integral tab, to expose the separated cells on the top surface thereof for ready microscopic examination.

An apparatus and method for ultrafast real-time optical imaging that can be used for imaging dynamic events such as microfluidics or laser surgery is provided. The apparatus and methods encode spatial information from a sample into a back reflection of a two-dimensional spectral brush that is generated with a two-dimensional disperser and a light source that is mapped in to the time domain with a temporal disperser. The temporal waveform is preferably captured by an optical detector, converted to an electrical signal that is digitized and processed to provide two dimensional and three dimensional images. The produced signals can be optically or electronically amplified. Detection may be improved with correlation matching against a database in the time domain or the spatial domain. Embodiments for endoscopy, microscopy and simultaneous imaging and laser ablation with a single fiber are illustrated.

A method and apparatus of automatically reprocessing a specimen for microscopic examination is disclosed. Processing of a specimen for microscopic examination involves fixation of the specimen and preparation of the embedded specimen from the fixed specimen. There are instances where, once a specimen has been processed, it is necessary to reprocess the specimen due to contamination of reagents during processing or inadequate fixation. The system automatically reprocesses a specimen by removing residual embedding material from the specimen with a clearing agent, removing the clearing agent with a dehydrating agent, and removing the dehydrating agent with an aqueous fluid.

A scanning probe microscopy (SPM) inspection and / or modification system which uses SPM technology and techniques. The system includes various types of microstructured SPM probes for inspection and / or modification of the object. The components of the SPM system include microstructured calibration structures. A probe may be defective because of wear or because of fabrication errors. Various types of reference measurements of the calibration structure are made with the probe or vice versa to calibrate it. The components of the SPM system further include one or more tip machining structures. At these structures, material of the tips of the SPM probes may be machined by abrasively lapping and chemically lapping the material of the tip with the tip machining structures.

The invention discloses a paraffin section method for fern gametophytes, which comprises the steps of material selection and fixation, washing, coloration and bluing, dehydration and hyalinizing, waxing and embedding, sectioning, patching, dewaxing and mounting, as well as microscopic examination and photographing, wherein improved FAA (formalin, acetic acid and alcohol) stationary liquid is adopted in the fixation, and the stationary liquid consists of formalin, acetic acid and 30% alcohol (formalin:acetic acid:30% alcohol = 1:1:18). The method is adopted, so that the disadvantage of much youngness of the fern gametophytes can be effectively overcome; the fixation can be well performed for cell divisions at all stages; from a paraffin section in a later stage, a plurality of mitotic sections can be observed, so that a whole development process can be reflected detailedly; therefore, the method has high development and application values.

The present invention relates to a robotic microscopy apparatus that is able to screen, detect, count and image in an automated and high throughput fashion whole multicellular organisms, tissues, individual cells and groups of cells on or embedded within agar, collagen or other defined matrix. To achieve this, the robotic apparatus of the invention images the samples from the top using a microscope with a long working distance. The invention provides robotic systems for plate handling, biological sample immobilization and microscopic examination. The invention also provides for automatic image acquisition, image storage and display, and image analysis.

An endoscope assembly is disclosed having a housing adapted to be manipulated by medical personnel, such as a surgeon. An elongated lens tube has one end secured to the housing while an elongated stage is removably secured to the housing so that the stage encompasses and is coaxial with the tube. The stage together with the lens tube are adapted for insertion into the cavity of a body. A lens assembly provided within the lens tube relays the optical image from the free end of the stage to the housing. A lens assembly within the housing, furthermore, varies the magnification of the image between macroscopic magnification and microscopic magnification in which tissue may be examined on a cellular level. For macroscopic magnification, white light is transmitted through the lens tube as well as reflected back from the target tissue through the lens tube and to the housing. For microscopic examination, laser radiation is utilized in lieu of the white light illumination. A line scanning confocal assembly contained within the housing enables microscopic examination of the target tissue at varying levels into the tissue from the end of the stage.

The invention relates to a method for fermentation production of a poultry bacterial agent by multi-bacteria miscible liquid, wherein aerobic bacteria, namely Bacillus subtilis, Bacillus licheniformis, bacillus natto, beer yeast, Aspergillus niger and Aspergillus oryzae are cultured in a shaking table according to different culture mediums, so as to culture a mother seed solution; simultaneously Lactobacillus acidophilus, bifidobacteria and enterococcus faecalis are subjected to anaerobic culture by utilization of Kille flasks, and a mother bacterial solution of photosynthetic bacteria is cultured by a Kille flask under the condition of illumination; the prior stock solution is inoculated into a seed tank for anaerobic culture and fermentation according to 4 percent of the inoculum concentration, and the fermentation time is between 48 and 60 hours; and the cultured seed liquid is inoculated into a productive tank for anaerobic culture and fermentation according to 10 percent of the inoculum concentration, the fermentation time is between 60 and 72 hours, and the fermentation end point is reached when the pH value is reduced to 4.0. The method has the advantages that the microscopic examination viable count of the bacterial agent is more than 5 billion per milliliter, so that the bacterial agent is safe and nontoxic, thereby not only improving the disease resistance of poultry but also promoting the quick growth of the poultry, reducing the feed-meat ratio and improving the quality of meat, eggs and milk.

The invention relates to a coverslipping module for mounting coverslips onto specimen slides (12) for microscopic investigations, having a transport apparatus (9) for transporting a specimen slide (12) out of a rack (3) or a specimen slide holder to a coverslipping position (13) in which the specimen slide (12) is equipped with a coverslip, and having alignment means for aligning the specimen slide (12) in the coverslipping position (13) for mounting the coverslip. In order to allow the specimen slide (12) to be reliably and quickly aligned for mounting of the coverslips, thereby ensuring a high level of process dependability with a short processing time, the alignment means are constituted by multiple movable orientation jaws (18) which are arranged so that by closure of the orientation jaws (18), the specimen slide (12) becomes aligned in the coverslipping position (13).

This application discloses a new method to record an image. It relates to microscopy and surface analysis in many scientific and technical fields. In particular it relates to the imaging and inspection of surfaces used in microelectronics: non-patterned and patterned wafers and photomasks. In the sense that it records electric amplitude, it relates to holography. Applications include microscopy, defect inspection, scatterometry, and optical metrology.

A method for autofocusing in microscopic examination of a specimen located at the focus of a microscope objective uses an autofocus beam path, the autofocus beam path being directed, via a deflection device arranged on the side of the microscope objective facing away from the specimen, toward the microscope objective, and from there onto a reflective autofocus interface in the specimen region. The autofocus beam path is reflected at the autofocus interface and directed via the microscope objective and the deflection device toward an autofocus detector. The deflection device comprises two regions spaced apart from one another in a propagation direction of the autofocus beam path. Each region reflects the autofocus beam path. The autofocus detector is arranged in a plane conjugated with the microscope objective pupil to acquire an interference pattern. The focus of the microscope is adjusted as a function of the acquired interference pattern.

An integrated interferometric and intensity based microscopic inspection system inspects semiconductor samples. A switchable illumination module provides illumination switchable between interferometric inspection and intensity based microscopic inspection modes. Complex field information is generated from interference image signals received at a sensor. Intensity based signals are used to perform the microscopic inspection. The system includes at least one illumination source for generating an illumination beam and an integrated interferometric microscope module for splitting the illumination beam into a test beam directed to the semiconductor sample and a reference beam directed to a tilted reference mirror. The beams are combined to generate an interference image at an image sensor. The tilted reference mirror is tilted with respect to the reference beam that is incident on the mirror to thereby generate fringes in the interference image. The system also includes an image sensor for acquiring the interference image from the inteferometric microscope module and intensity signals from the microscopic inspection image.

The invention discloses a method for detecting the plastic content of a marine organism. The method is characterized by including the steps: drying, crushing and digesting marine organism digestive tracts or muscular tissues; performing density gradient centrifugation for digestion solution; performing microscopic examination for micro-plastics in obtained centrifugal liquid by a fluorescence microscope and a Fourier transform infrared microscope; counting the number of the micro-plastics. The method is simple to operate, high in accuracy and application safety and applicable to marine organism micro-plastic pollution detection.

An apparatus and method for ultrafast real-time optical imaging that can be used for imaging dynamic events such as microfluidics or laser surgery is provided. The apparatus and methods encode spatial information from a sample into a back reflection of a two-dimensional spectral brush that is generated with a two-dimensional disperser and a light source that is mapped in to the time domain with a temporal disperser. The temporal waveform is preferably captured by an optical detector, converted to an electrical signal that is digitized and processed to provide two dimensional and three dimensional images. The produced signals can be optically or electronically amplified. Detection may be improved with correlation matching against a database in the time domain or the spatial domain. Embodiments for endoscopy, microscopy and simultaneous imaging and laser ablation with a single fiber are illustrated.

A flow chamber (1) made of plastics as an object carrier for light-microscopic examinations comprised at least one channel (4) in a base plate (2), said channel having a width of 0.01 to 20 mm and a height of 0.01 to 5 mm. One liquid reservoir (3, 3′) each is connected to the inlet and outlet opening (6, 6′) of the channel, whereby a communicating system is generated. The bottom and / or the cover of this chamber is made of a high-class plastic material and may be functionalized. The inlet and outlet portions of the channel may be formed by a rounding (7) of the channel edges or by a surface treatment in a manner that drop formation is prevented and the flow is therefore not obstructed. In a method of sample preparation for light-optical microscopic examinations, a sample flow is generated by a system of communicating pipes, in that a reservoir of a solution with the sample is connected via a third channel with at least one further reservoir and the filling level of the reservoirs differs at the beginning of the examination.

Hydroxyapatite (HA)-binding peptides are selected using combinatorial phage library display. Pseudo-repetitive consensus amino acid sequences possessing periodic hydroxyl side chains in every two or three amino acid sequences are obtained. These sequences resemble the (Gly-Pro-Hyp)x repeat of human type I collagen, a major component of extracellular matrices of natural bone. A consistent presence of basic amino acid residues is also observed. The peptides are synthesized by the solid-phase synthetic method and then used for template-driven HA-mineralization. Microscopy reveal that the peptides template the growth of polycrystalline HA crystals ˜40 nm in size.

The invention relates to a hatching device and method for demersal spawns of marine fishes. The hatching device comprises a net cage, a water channel, a bracket, a temperature controller indicator, a probe, a heating rod, a water inlet hose, a sinker, an air inlet hose, an air stone and an annular water injector, wherein the net cage and the bracket are located in the water channel; the net cage is bound on the bracket; the water inlet hose and the air inlet hose are located in the water channel outside the net cage; the upside of the net cage is provided with the annular water injector; and the probe and the heating rod are arranged in the water channel. The hatching method comprises the following steps of: controlling a hatching condition; hatching; carrying out microscopic examination observation; carrying out secondary water temperature controlling; managing routinely; collecting larva fish and the like. The hatching method has the advantages that spawn deposition is avoided because of conical point inflation; spawns adhered on net cage edges are washed at any time by the annular water injector; the net cage is conveniently replaced; the water temperature is stably controlled by an electric temperature controller; and the fresh water quality can be ensured due to water feeding and drainage modes of water feeding of the annular water injector at the upside and water feeding of the water channel at the bottom and overflowing of the periphery of the water channel. Therefore, by means of the hatching device and method, a hatching rate of the demersal spawns of the marine fishes in the industrial fry breeding is improved.

A device, method and system for preparing and storing samples for microscopic analysis is disclosed. The device provides a reservoir that can be attached to a displacement pipette thereby filling the reservoir with reagents desired for preparing the samples for microscopic analysis. In some embodiments, the specimen may be contained on a transmission electron microscope (TEM) grid. In other embodiments, the sample may be a light microscope (LM) specimen or a scanning electron microscope (SEM) specimen. In yet another embodiment, the invention provides a method of preparing samples for microscopic examination including a device for preparing TEM grids with, a device for preparing TEM, SEM or LM specimens with and a device for storing both grids and specimens in. In yet another embodiment, the invention provides a system for tracking the preparation, analysis and histological evaluation of multiple samples while also providing for their long term storage.

The present invention is a method for determining the location of and distinguishing aggressive diamonds from active diamonds on a diamond conditioner disc, comprising: (a) contacting a diamond conditioner disc with a hard surface, wherein the diamond-containing side of the diamond conditioning disc is facing the hard surface, (b) pushing the conditioner disc a sufficient distance that all diamonds could possibly be scratching the surface at the same time and at least a distance corresponding to the length of the said diamond conditioner disc (c) observing number and position of the scratches left by diamonds on the hard surface to determine the number and position of active diamonds on the diamond conditioner disc, and (d) selecting the diamonds, the marks for which are the most pronounced and which comprise 50% or more of the total furrow area observed for all of the active diamonds in descending order of furrow are plus any diamonds in excess of the number needed to achieve said 50% or more whose individual furrow area is 2% or more, which diamonds are determined to be aggressive diamonds, or impressing the diamond conditioner disc under a load onto a hard surface and the impression of the most aggressive diamonds in the hard surface being confirmed by microscopic examination to in turn confirm the position and aggressiveness of the aggressive diamonds observed or (e) contacting a diamond conditioner disc with a hard surface, wherein the diamond-containing side of the diamond conditioning disc is facing the hard surface, (f) pushing the conditioner disc a sufficient distance that all diamonds could possibly be scratching the surface at the same time and at least a distance corresponding to the length of the said diamond conditioner disc (g) observing number and position of the scratches left by diamonds on the hard surface to determine the number and position of active diamonds on the diamond conditioner disc, (h) the hard surface further comprises a layer of contrasting material such that when the diamond conditioner disc moves across the hard surface, the said diamond conditioner disc crosses the limits of the layer entirely from one end to the other and scratches the layer of contrasting material on the hard surface thereby leaving a visible mark, (i) the said layer is between 8 and 15 microns thick and (j) selecting the diamonds which cut entirely through the said layer allowing backlighting to be easily viewed.

Methods are disclosed for identifying and treating oil sand ores having degraded bitumen. This can be done by considering the location from which the ore is mined or its history, or by microscopic examination of a bitumen froth made from the ore, or by near-infrared spectroscopy. Ore which contains degraded bitumen is treated with at least 0.05 wt / wt % alkaline material, preferably 0.1 wt. / wt % of such alkaline material, in the water addition step of a hot or warm water extraction process for the making of bitumen froth, such as the Clark process.

The invention discloses a method for detecting density distribution of micro-plastics in soft tissues of marine organisms, and belongs to the field of micro-plastics in fish or bivalve organism samples. The method for detecting the density distribution of the micro-plastics in the soft tissues of the marine organisms comprises the following steps of selecting the fish or bivalve organisms with similar individuals, washing the fish or bivalve organisms with pure water to prepare digestive tract or soft tissue samples, and recording the weights; preparing a digestion solution for digesting the prepared samples, and obtaining a mixed digestion solution; adding NaCl into the mixed solution to carry out density flotation; and carrying out microscopic examination on particles subjected to flotation, selecting the suspected particles to be subjected to analysis of a microscopic-Fourier spectrometer and the like, performing counting after the particles are identified as the micro-plastics, andperforming calculation to obtain the density distribution of the micro-plastics. According to the method, the micro-plastics in the digestive tracts or the soft tissues of the marine organisms can beaccurately quantified, thereby providing basic data for the pollution condition of the micro-plastics in an area.

The invention relates to a screening method for producing chondroitin sulfate bacillus subtilis and application of a bacterial strain fermentation method in the production of chondroitin sulfate, belonging to the technical field of biological engineering. The bacterial strain disclosed by the invention is bacillus subtilis which is obtained by the following steps of: diluting and coating fermented soya beans subjected to a boiling water bath, dyeing and carrying out microscopic examination on an obtained soporiferous strain, then carrying out primary fermentation and shaking culture one by one on screened bacterial strains, adding chondroitin sulfate standard substance of sharks in a formation liquor to be used as an internal standard, carrying out qualitative analysis and screening by adopting a high performance liquid chromatography, and carrying out morphological, physiological and biochemistric and molecular biological identification on the screened bacterial strains to obtain thebacillus subtilis for producing the chondroitin sulfate. Meanwhile, the invention discloses a method for producing the chondroitin sulfate by using the bacterial strain; and the method is used for fermenting the chondroitin sulfate for 24h, wherein the yield of the chondroitin sulfate is 177mg / L.

The present invention provides Bacillus vallismortis SZ-4, which has the preservation number of CGMCC No.8273. According to the present invention, the strain grows on a beef extract-peptone (NB) culture medium in a single cell reproduction growth manner to form bacterial colonies, and the bacterial colonies have characteristics of round shape, milk white color, slightly elevated center, and moist and transparent surface; microscopic examination results show that the Bacillus vallismortis SZ-4 has a rod shape, has flagella, has a size of 0.7-0.82*1.6-2.2 mum, presents the positive Gram staining effect, has spores, and can grow on a beef extract-peptone (NB) culture medium containing 2-5% of NaCl, wherein the beef extract-peptone (NB) culture medium comprises: 3.0 g of a beef extract, 10.0 g of peptone, 5.0 g of NaCl, 17 g of agar and 1000 ml of water, and the pH value is 6.8-7.2; and the Bacillus vallismortis SZ-4 provides significant antagonism effects for pathogenic bacteria causing ginseng root rot, blight, sclerotinia sclerotiorum and botrytis cinerea. The present invention further provides an application of the Bacillus vallismortis SZ-4 in prevention and control of plant fungal diseases or preparation of microorganism preparations for prevention and control of plant fungal diseases.

A quantitative fecal examination apparatus includes a fecal collector removably mounted on a container base, having the fecal collector provided with a suction portion for sucking a fecal specimen into the collector and then the fecal specimen is disposed into the container base for examination test or for sucking the fecal specimen for further laboratory test including microscopic inspection, and having a quantitative design in the examination apparatus for collecting the fecal sample in a pre-set quantity for more precise examination.