223 results about "Paraffin section" patented technology

The invention discloses a manufacturing method of paraffin sections of zostera marina embryo, comprising the following steps: (1) material drawing and fixation: zostera marina seeds are taken and the fruit skin is stripped, and after endosperm is removed, an integral zostera marina embryo is soaked in FAA (free amino acids) fixing solution and is fixed for more than 48 hours; (2) dehydration: the zostera marina embryo is taken out from the fixing solution and is soaked in alcohol for dehydration; (3) waxing and embedding: paraffin is added gradually in a vessel containing the zostera marina embryo and dimethylbenzene, and conventional paraffin embedding is carried out to obtain the wax blocks containing the zostera marina embryo; (4) slicing, spreading and drying: the wax blocks coated with the zostera marina embryo is fixed on a wheel rotation type slicing machine for carrying out continuous slicing of the paraffin, so as to obtain wax bands and enable the wax bands to be attached to an object slide; (5) dewaxing, rehydration and dyeing of hematoxylin dye solution; (6) slicing, dehydration and dyeing of eosin and alcohol; and (7) transparence and mounting are carried out to manufacture a permanent section. In the manufacturing method disclosed by the invention, the paraffin sections of the zostera marina embryo, which have clear dyeing and an integral texture structure, can be obtained.

The invention discloses a paraffin section method for fern gametophytes, which comprises the steps of material selection and fixation, washing, coloration and bluing, dehydration and hyalinizing, waxing and embedding, sectioning, patching, dewaxing and mounting, as well as microscopic examination and photographing, wherein improved FAA (formalin, acetic acid and alcohol) stationary liquid is adopted in the fixation, and the stationary liquid consists of formalin, acetic acid and 30% alcohol (formalin:acetic acid:30% alcohol = 1:1:18). The method is adopted, so that the disadvantage of much youngness of the fern gametophytes can be effectively overcome; the fixation can be well performed for cell divisions at all stages; from a paraffin section in a later stage, a plurality of mitotic sections can be observed, so that a whole development process can be reflected detailedly; therefore, the method has high development and application values.

This invention relates to body liquid cell olefin slice making process, which comprises pre-processing, decenter deposition and fixing dehydrate wax impregnation, burying, slicing and HE dying and sealing. The invention works together with daily work with clear background of HE and immune organization dying without dark background of agar burying and processing the molecule burying and improves the accuracy of the body liquid cell diagnose.

The invention discloses a production method for a paraffin section of a paeonia lactiflora mature embryo. The production method comprises the following steps: drawing materials and fixing: stripping a seed coat, cutting a seed, immersing the seed top part containing the embryo or part endosperm into a FAA stationary liquid, and fixing for more than 24 hours; dehydrating: washing in ethyl alcohol with the concentration of 70% for 3 to 5 times, and performing gradient dehydration from the ethyl alcohol with the concentration of 70%; performing hyalinization: carrying out five level hyalinization by using dimethyl benzene as a transparent reagent; waxing and embedding: pouring out one half volume of dimethyl benzene, adding wax crumbs in a bottle until saturation, staying overnight at the temperature of 40 DEG C; then changing a wax liquid every two hours for three times per day, repeating for three days, and carrying out conventional paraffin embedding; slicing; flattening and bonding: flattening a linoleum tape on an iron plate of a water bath kettle at the constant temperature of 50 DEG C, dewaxing, rehydrating, dyeing through safranine and fast green and performing hyalinization; mounting. According to the invention, the paeonia lactiflora mature embryo paraffin section of which the tissue structure is integrated and clear can be obtained, so that accurate technical support can be provided for researching paeonia lactiflora seed dormancy and germination.

The invention discloses a probe and a sequence combination for simultaneous detection of various mutation types and particularly provides a kit, a method, a gene chip, a probe and a sequence combination which are high in sensitivity and specificity and used for simultaneous detection of point mutation, short segment insertion and deletion, copy number variation and fusion genes of 57 tumor driver genes listed in a detection list. The invention further discloses a sequence combination and probe associated gene chip and kit and a method for simultaneous detection of various mutation types. By detection of body fluid including blood, hydrothorax, abdominal dropsy and the like and tumor frozen tissues or paraffin sections, comprehensive tumor driver gene mutation information can be acquired, sensitivity and accuracy in tumor gene detection are improved, and accordingly clinicians can be assisted in making of individualized medication schemes to achieve best accurate treatment effects.

A mortar powder containing paraffin wax for phase-change insulating is prepared from cement, light aggregate, fibres and paraffin wax through fusing paraffin wax or preparing the microcrystal powder of paraffin wax, and proportionally mixing it with others. Its advantages are low hydroscopicity and high water-proof performance and softening coefficient.

A process for the production of paraffin sections of biological tissue, especially for molecular pathology studies is disclosed. In the process, the tissue sample is simultaneously fixed, dehydrated and cleared in a first step, subsequently dehydrated and cleared in a second step and infiltrated with an inert specimen matrix in a third step. The specimen can then be further embedded in a casting supporting matrix according to the standard procedures followed by any local pathology or research laboratory. A kit and a processing station for automating paraffin embedding of a tissue sample suitable for histopathological and molecular analysis is also described. A bio-indicator system is described for measuring the degree of crosslinking. A tissue sample holding means or a vial which includes a tissue sample holding means provided with a data logging device capable of registering and transmitting data regarding the sample and conditions where the sample was processed is also disclosed.

A green making technology of animal tissue paraffin section disclosed in the invention aims at providing a technique of making a paraffin section of animal tissue applied to the agricultural university medicine and other majors, characterized by that the type of chemical reagents that are used is limited, the chemical reagents have no toxicity, and dehydrate the tissue thoroughly, accelerate making sections but not lead to excessive contraction and increasement of hardness of the tissue, and substitute dimethyl benzene used in the traditional making technology of the paraffin section which isharmful to human body, so as to eliminate the occupational diseases and environmental pollution caused by dimethyl benzene. The making technology comprises the following steps: carrying out fixation with a fixative solution (100ml of the fixative solution comprises 91 ml 85% alcohol, 4 ml methanol and 5 ml glacial acetic acid ), dehydrating the tissue with graded ethanol, then putting the fixative and dehydrated tissue in a mixed transparent reagent (comprising 14wt% of analytically pour n-butanol, 29wt% of acetone and 57wt% of absolute alcohol ) for standing for 3 hours, then carrying out normal waxing and embedding, using analytically pour turpentine (60 DEG C) for section staining, carrying out dewaxing twice, carrying out hydration under normal temperature, using haematoxylin for staining nuclei, decoloration and bluing, and using eosin for staining, dehydrating until get absolute alcohol, then carrying out air drying, and directly using the gum diluted by turpentine to sealing the section.

A method for preparing paraffin section of seawater roe includes selection of fixing agent, confirmation of dewatering process, confirmation of transparent process, confirmation of wax penetration process, improvement of dyeing mode. In the method, egg capsule stripping process being difficultly operated is omitted.

The invention discloses a full-field optical coherence tomographic three-dimensional medical imaging device and a full-field optical coherence tomographic three-dimensional medical imaging method. The device comprises a Kohler illumination part, a reference arm, a sample arm, an imaging lens and an area array CCD detector; according to the method, piezoelectric ceramics are adopted for phase shift of a reference mirror according to the white light low-coherence interference theory; the optical path of the two arms is changed; CCD is used for receiving an interference signal; the interference signal is processed by a computer to obtain a two-dimensional fault map of a sample; finally an electric control displacement platform is used for axial scanning to obtain three-dimensional information, wherein a single chip can be used for emitting two different signal modulation piezoelectric ceramics; the CCD and the electric control displacement platform for the two arms correspond to two demodulation modes. According to the device and the method disclosed by the invention, the sample needs not to be subjected to the treatments such as slicing, dyeing and fluorescence labeling; frozen sections and paraffin sections which have the defects such as complex preparation and low resolution ratio can be replaced; a handhold medical instrument can be made for submicron-grade in-vitro imaging or in-vivo imaging of biological tissues.

The present invention relates to a microtome (1) having functional regions to be operated manually, in particular a sample holder (2), a cutting device (3), a section removal system (4), and a section collection pan (5). A microtome (1) is described in which the particularly firm contact or adhesion of sectioning waste fragments and, in particular, of thin sections can be at least largely avoided. For this purpose, the microtome (1) according to the present invention is characterized in that the components of the microtome (1) that can be brought into contact with paraffin sections are embodied in electrically conductive fashion.

A process and kit for the production of paraffin sections of biological tissue, especially for molecular pathology studies, is described. In the process, the tissue sample is simultaneously fixed, dehydrated and cleared in a first step, subsequently dehydrated and cleared in a second step and infiltrated with an inert specimen matrix in a third step. The specimen can then be further embedded in a casting supporting matrix according to the standard procedures followed by any local pathology or research laboratory. The method is uncomplicated, requires little hands-on time, is reproducible and is standardized thereby limiting cross-centre pre-analytical variance. A bio-indicator system for measuring the degree of crosslinking is also disclosed.

The invention discloses a preparation method for a gill tissue paraffin section. The preparation method comprises the following steps: fixing, decalcifying, dehydrating, transparentizing, carrying out paraffin permeation, embedding, slicing, sticking sections, expanding the sections, de-waxing and rehydrating, staining, re-staining, sealing and the like. Compared with an existing paraffin section manufacturing method, an operation process of dehydrating, transparentizing and immersing by wax is improved; the preparation fixing and tissue wax immersing effects of gill tissue paraffin are improved; a slicing problem when the gill tissue paraffin section is prepared is improved; the structure definition of the gill tissue section is greatly improved; a plurality of problems in a gill tissue manufacturing process in the prior art are solved. The preparation method is good for antigen positioning of immunocytochemical staining, so that when experiments including in-situ hybridization, immunohistochemistry, immunofluorescence and the like are carried out on the gill tissue paraffin section, tissue distribution and cell positioning of some genes and proteins can be displayed, and further feasible conditions are provided for carrying out gill research on levels of cells, genes and proteins.

The invention relates to a method for producing an early gonad paraffin section of Chinese soft shell turtle, which comprises the following steps: 1)fixing a sample, 2)dehydrating and transparentizing, 3)performing paraffin infiltration and embedding, 4)slicing and spreading the section, and 5)dyeing and sealing the section. The method is characterized in that the sample in the step 1) is embedded in a bean product pretreated by a stationary liquid. Compared with prior art, the method has the advantages that the bean product can be taken as a pre-embedding material for embryo stage gonad paraffin section of Chinese soft shell turtle, the source is wide, cost is low, through the pre-embedding, the embryo stage gonad can be better embedded in bean product, and subsequent tissue embedding and slicing are not influenced. The method avoids the disadvantages in a traditional method, and overcomes the disadvantages of difficult operation and observation of early gonad paraffin section of Chinese soft shell turtle; in addition, the pre-embedding gonad position is fixed and several gonad tissue can be processed at a time, so that large scale slicing and analysis are convenient to operate, and the method is especially suitable for researches by aiming at different individuals.

The invention discloses a multi-antibody detection kit used in postoperative recurrence risk assessment after a resection operation is performed on a primary hepatocellular carcinoma patient. The kit comprises 8 immunohistochemical antibodies used for paraffin sections. The invention also discloses a method for determining postoperative recurrence risk of a liver cancer patient by using the kit. The prediction effect provided by the invention is superior to an existing clinical index. The kit and the method provided by the invention have characteristics of objective, convenient, direct, and repeatable. The kit can be used in the predictions of recurrence and death after clinical liver cancer resection operations. The kit assists in screening liver cancer patients with high postoperative recurrence risk. Therefore, appropriate auxiliary treatments can be directed, recurrence can be effectively reduced, and patient survival time can be prolonged.

The present invention relates to cotton tissue specific and pathogenic bacterium inducing promoter and their application, and belongs to the field of biotechnology. The promoter contains CAAT-box, TATA-box, ethylene, methyl jasmonate, abscisic acid response element, pathogenic bacterium inducer response elements W boxes, GT-1, MYB, MYBST1, MYB1LEPR, etc. There are also specific root expressing elements to enhance the expression of GUS gene, which expresses specifically in the phloem of root, bud and stem, the vein of foliage and the guard cell of air pore. Paraffin section observation shows that GHNBS promoter expresses in phloem companion cell. After treatment with SA, MeJA, Ethylene, pathogenic bacteria of blight and pseudomonas syringae DC3000, GUS will have obviously raised activity, so that it is one organ specific and pathogenic bacterium relevant promoter.

The invention relates to a method for extracting desoxyribonucleic acid from formalin fixed and paraffin embedded tissues, and belongs to the technical field of nucleic acid application in biology. The method comprises the following steps of: preparing special lysis solution, adding the lysis solution into paraffin section tissues, boiling at high temperature for 30 minutes, and centrifuging to take supernate; adding absolute ethanol for uniform mixing, and adding the mixed solution into a silicon membrane absorption column for centrifuging; rinsing by using protein-free liquid and salt-free rinsing liquid; and eluting by using elution buffer. In the method, a toxic reagent of dimethylbenzene is not used for dewaxing, and harm to bodies of experimenters is avoided; and precious protease Kis not needed to perform long-time incubation enzymolysis, the operation is simple and quick, the extracted desoxyribonucleic acid in genome is high in quality and stability, and the cost and time can be saved to the greatest degree. The extracted product is subjected to polymerase chain reaction (PCR) detection, long segments with about 750pb can be obtained through amplification, and the work in the aspects such as scientific research, biomedicine and the like is greatly facilitated.

The invention belongs to the technical field of biological nucleic acid detection, and particularly relates to a rapid nucleic acid extraction kit and applications thereof. The kit of the invention comprises a grinding rod, an extraction liquid, an extraction column, a micro ultra-filter and a centrifuge tube, wherein the extraction liquid is a DEPC aqueous solution containing chelex-100. The invention further relates to a use method for the kit, and applications of the kit in extractions of DNA or RNA from pathological tissue paraffin section samples, samples of blood stains and semen stains, tissue fluid samples, cerebrospinal fluid samples, blood samples, serum samples, plasma samples, urine samples, hydrops samples, and tissue cell samples. According to the present invention, content and quality of the nucleic acid extracted by the kit of the present invention can well meet requirements of follow-up reactions of PCR, RT-PCR and sequencing. With the present invention, limitation of complexity and time consuming of nucleic acid extraction in the prior art are overcome well, and characteristics of safety and environmental protection are provided.

This invention disclosed a de-waxing method used for paraffin-cut section. De-waxing solution was prepared by mixing distilled water and Triton X-100 by volume ratio of 99:1. It can be used by the following procedure: place the slice into the first de-wax solution and dip for 10-2 minutes at 60-70DEG C; take it out and place it to the second de-wax solution and repeat the above operation; wash it by water. The solution Triton X-100 used in this invention was safe, cost save, environmental friendly and it's easy to handle.

The invention relates to application of a fluorescent in-situ hybridization polyclonal separating probe for renal carcinoma and a kit thereof. Clonal fragments used by the polyclonal separating probe provided by the invention are respectively a combination of RP11-416B14, CTD-2522M13 and CTD-2516D6, and a combination of CTD-2312C1, CTD-2248C21 and RP11-959H17. According to the invention, the defect that karyotype analysis can not be performed conventionally after tumor cells are subjected to cell culture until a mitotic phase in the prior art is overcome. The typical gene modification in Xp11.2 translocation / TFE3 gene fusion related renal carcinoma is detected by using the FISH technology, thereby diagnosing the tumor. The fluorescent in-situ hybridization polyclonal separating probe application is high in accuracy, high in specificity, high in success rate, strong in fluorescent signals and convenient to operate. The invention can be applied in paraffin sections, widens the scope of detection specimens, and greatly optimizes the diagnosis method of Xp11.2 translocation / TFE3 gene fusion related renal carcinoma.

The invention relates to an ASPL-TFE3 fused renal carcinoma gene probe as well as an application of a kit thereof. Cloned fragments selected by the gene probe are respectively a combination of RP11-634L10, RP11-51H16 and RP11-475F12 and a combination of CTD-2311N12, RP11-416B14, CTD-2522M13, CTD-2516D6, CTD-2312C1, CTD-2248C21 and RP11-959H17. By using the ASPL-TFE3 fused renal carcinoma gene probe, the defects of complexity, time consumption and clinic application unsuitability of RT-PCR and cell karyotyping methods applied in the past are overcome. According to the ASPL-TFE3 fused renal carcinoma gene probe, a unique ASPL-TFE3 fused gene in ASPL-TFE3 fused renal carcinoma is detected by using an FISH technology, so that the renal carcinoma is diagnosed; the gene probe is high in accuracy rate, specificity and success rate, strong in fluorescence signal, simple and rapid in operation and rapid in diagnosis when applied and can be applied to paraffin section; and due to the application of the ASPL-TFE3 fused renal carcinoma gene probe, the specimen detecting range is widened, a novel method for accurately, reliably, simply and conveniently diagnosing the ASPL-TFE3 fused renal carcinoma is established, and the precedent that the ASPL-TFE3 fused renal carcinoma is detected by virtue of FISH is created.

A method of preparing a dactylis glomerata root tissue paraffin section is disclosed. The method includes a step of killing and immobilizing a fresh dactylis glomerata root tissue block, a step of washing, a step of dehydrating and hyalinizing, a step of wax soaking and embedding, a step of block trimming and sectioning, a step of section expanding and roasting, a step of dewaxing, rehydrating and staining with safranine, a step of staining with fast green, dehydrating and hyalinizing and a step of section sealing. After the step of washing and before the step of dehydrating and hyalinizing, agarose pre-embedding is performed and includes embedding the washed dactylis glomerata root tissue block into agarose gel having a content of 5% by mass and melt by stewing. The dactylis glomerata root tissue paraffin section prepared by the method is complete in tissue, clear in cell boundary, bright in color, sharp in color contrast and clean in sectioning.

The invention discloses a kit and a method for rapidly releasing nucleic acid from paraffin-embedded tissue sections. The kit comprises a proteinase K solution (prepared from 10-100g / L protease K, 10-200mM / L Tris with pH value of 7.0-11.0 and 0.01-5mM / L anhydrous calcium chloride), a first nucleic acid release reagent (1-1000 mM / L Tris with pH value of 7.0-11.0, Triton X-100 with a volume ratio of0.1 to 2%, Nonidet P-40 or substitutes thereof with a volume ratio of 0.1 to 2%, and 0.1-10mM / L EDTA disodium salt), and a second nucleic acid release reagent (10-200mM / L Tris with pH value of 7.0-8.0, and Nonidet P-40 or substitutes thereof with a volume ratio of 0.1- 2%). The kit is also preferentially provided with a centrifuge column; when the kit is in use, the paraffin section is placed ina centrifuge tube, the first nucleic acid release reagent and the proteinase K solution are added into the centrifuge tube, the materials in the centrifuge tube are fully and evenly mixed, and incubation is carried out for the first time; after that, the second nucleic acid release reagent is added and is evenly mixed in a way of vortex, and incubation is carried out for the second time, so that asplit product is obtained; the product is then filtered by means of the centrifuge column, so that target nucleic acid can be obtained. After the kit and the method are adopted, the paraffin sectionsdo not need to be subjected to dewaxing, and the complicated purification step is omitted. The kit and the method have the advantages of being safe to use, simple and convenient to operate, high in recovery efficiency and lower in manufacturing cost.

The invention provides a fluorescence PCR detection kit for human K-RAS gene mutation. The kit comprises a forward primer sequence K-M1 PCR reaction solution, a forward primer sequence K-M2 PCR reaction solution, a forward primer sequence K-M3 PCR reaction solution, a forward primer sequence K-M4 PCR reaction solution, a forward primer sequence K-M5 PCR reaction solution, a forward primer sequence K-M6 PCR reaction solution and a forward primer sequence K-M7 PCR reaction solution which are used for detecting Gly12Asp mutation, Gly12Val mutation, Gly12Ser mutation, Gly12Cys mutation, Gly12Ala mutation, Gly12Arg mutation and Gly13Asp mutation on a codon 12 and codon 13. According to the kit, under the 100ng / reaction wild-type human DNA background, amplification signals and the false positive condition are avoided, and it shows that the kit is good in specificity and high in antijamming capability. By the adoption of the kit, human K-RAS gene mutation in samples such as clinical paraffin sections can be detected quickly, and a reliable experiment basis is provided for diagnosing related cancer therapies.

The invention relates to the field of detection by biotechnology, and aims at providing a visualized in-situ hybridization detection kit with high sensitivity and reliability and a method thereof for human papilloma virus. The invention has one technical scheme of providing an in-situ hybridization detection kit for human papilloma virus, comprising a slide, a hybridization solution and an in-situ hybridization reagent, wherein the hybridization solution comprises a labeled hybridization probe, and the sequences of the hybridization probe are as shown in SEQ ID NO:9 to SEQ ID NO:12. The invention has the beneficial effects of overcoming the limitation that an in-situ hybridization detection sample has to be a paraffin section or frozen section by special treatment of the slide, introducing a hybridization enhancing solution and a development synergic solution in the in-situ hybridization detection procedure, increasing the signal / noise ratio of the detection result, and facilitating reading.

The invention relates to the biochips field, in particular to a tissue chip used for functional genome research and the preparation method as well as the application thereof. The tissue chip of the invention includes a substrate and a paraffin section arranged on the surface of the substrate, wherein, the paraffin section is provided with tissue sample points which are in different development stages and array according to the tissue dynamic development process. Because the tissue chip of the invention can be combined with a gene chip technology, the space-time expression of a difference expressive gene screened out by the chip technology in the tissue dynamic development process is validated and researched in the high-quality, economic and available way.

The invention provides a tissue paraffin removing liquid, which includes vegetable oil. The paraffin removing liquid not only can be used for manually removing paraffin from tissue paraffin section in a routine laboratory, and but also can be used for on-line paraffin removing of the tissue paraffin section on an automatic dyeing instrument. The tissue paraffin removing liquid can completely replace xylene during the paraffin removing process, so as to achieve excellent dyeing effect and have the paraffin removing same effect as the xylene. The natural vegetable oil as the main component of the paraffin removing liquid is harmless to human and environment.

The invention relates to a detection method of prostatic cancer related fusion gene Fish. The detection method comprises the steps of: carrying out conventional pretreatment on prostatic cancer tissue paraffin sections, then carrying out in-situ hybridization with a two-color fusion probe, and judging whether the prostatic cancer related fusion gene exists according to the detected fluorescence signal. Preferably, the two-color fusion probe is TMPRSS2 / ERG two-color fusion probe, TMPRSS2 / ETV1 two-color fusion probe or TMPRSS2 / ETV4 two-color fusion probe, wherein the TMPRSS2, ERG, ETV1 and ETV4 are respectively nucleotide sequences shown in SEQ ID NO: 1-4. The invention also relates to a correlated two-color fusion probe and a kit. The detection method of the prostatic cancer related fusion gene Fish is ingenious in design, is simple to operate, greatly increases specificity and sensitivity, can be used for quickly, accurately and visually detecting the prostatic cancer related fusion gene, and has accurate and reliable detection result, thus the detection method can be used as a reference for diagnosing prostatic cancer by physicians, and is suitable for large-scale popularization and application.

The method of the invention comprises the steps of: (1) collecting health coral containing a hermatypic coral oocyte in seafloor; (2) fixing the coral; (3) removing a calcareous skeleton of the hermatypic coral; (4) removing moisture from the texture of the coral; (5) conducting hyalinization to the texture; (6) immersing paraffin into the texture so as to make preparation for sectioning; (7) revising the section; (8) fixing the section on a glass slide; (9) removing the paraffin from the section; (10) carrying out coloration; (11) performing dewatering and section sealing. Considering the characteristic of lime skeleton in hermatypic coral, the method of the invention solves the problem that the lime skeleton of hermatypic coral is unsuitable for making paraffin sections by applying formic acid decalcification method and the like, thus providing accurate technical support for studying hermatypic coral oocyte growth and predicting the ovulation time of hermatypic coral.

The invention relates to a manufacture method of continuous paraffin sections for gonad of pelodiscus sinensis in embryonic period and application of the manufacture method in sex determination. The manufacture method comprises the following steps: (1) fixing a sample, (2) dying the gonad, (3) carrying out dehydration and transparentizing procedure, (4) absorbing paraffin and embedding, (5) cutting into sections and flatting sections, and (6) dying and mounting. The manufacture method is characterized in that the gonad is first dyed in the step (2); sections are subjected twice flatting by adopting water bath and high-temperature paraffin in the step (5). Compared with the prior art, the manufacture method has the advantages that the gonad is dyed first before being embedded, the gonads of a plurality of individuals are embedded in one paraffin block, determination is carried out through a manner of continuous tissue sections, and then the result accuracy is ensured. The manufacture method is simple to operate and convenient to popularize and apply. By utilizing the manufacture method, sex ratio of the pelodiscus sinensis in the embryonic period can be rapidly and accurately subjected to statistics.