2035 results about "Agarose" patented technology

The invention relates to a transdermal analyte monitoring system comprising a medium adapted to interface with a biological membrane and to receive an analyte from the biological membrane and an electrode assembly comprising a plurality of electrodes, wherein the medium is adapted to react continuously with the analyte, an electrical signal is detected by the electrode assembly, and the electrical signal correlates to an analyte value. The analyte value may be the flux of the analyte through the biological membrane or the concentration of the analyte in a body fluid of a subject. The medium may comprise a vinyl acetate based hydrogel, an agarose based hydrogel, or a polyethylene glycol diacrylate (PEG-DA) based hydrogel, for example. The surface region of the electrode may comprise pure platinum. The system may include an interference filter located between the biological membrane and the electrode assembly for reducing interference in the system. The system may comprise a processor programmed to implement an error correction method that corrects for sensor drift.

The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activiated agarose matricees, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.

The invention relates to a transdermal analyte monitoring system comprising a medium adapted to interface with a biological membrane and to receive an analyte from the biological membrane and an electrode assembly comprising a plurality of electrodes, wherein the medium is adapted to react continuously with the analyte, an electrical signal is detected by the electrode assembly, and the electrical signal correlates to an analyte value. The analyte value may be the flux of the analyte through the biological membrane or the concentration of the analyte in a body fluid of a subject. The medium may comprise a vinyl acetate based hydrogel, an agarose based hydrogel, or a polyethylene glycol diacrylate (PEG-DA) based hydrogel, for example. The surface region of the electrode may comprise pure platinum. The system may include an interference filter located between the biological membrane and the electrode assembly for reducing interference in the system. The system may comprise a processor programmed to implement an error correction method that corrects for sensor drift.

The invention is drawn to composite agarose / acrylamide compositions and gels. In particular it relates to gels for the separation of molecules, particularly macromolecules such as proteins. The invention is also directed to the preparation of composite gels, the separation of molecules by techniques such as electrophoresis using such gels, and the transfer of proteins from such gels to a transfer membrane using an immunoblot transfer gel.

A food product that includes a casing of a gelatin-free, water-based, set hydrocolloid gel that forms an enclosure, and at least one solid, liquid, soft or particulate center enclosed by the casing. The casing is made of a carageenan, alginate, agarose, gellan gum, pectin or cellulose compound, and the food product can withstand changes in temperature. Also, a process for the production of such a food product, wherein a liquid hydrocolloid mass is partially set and a hard, liquid, soft or particulate center is injected therein with the final completing or the setting of the hydrocolloid mass providing the encapsulation of the center.

A plugging medium is used to block the bottom opening of cassettes for vertical electrophoresis in order to facilitate filling the cassettes with separation media. Cassettes can be filled within a vertical electrophoresis system in which electrophoresis is conducted without further manipulation of the cassettes. The system includes a frame assembly mounted on a base containing a basin for plugging solution. The cassette is mounted to the frame assembly in a substantially vertical position such that a bottom opening of the empty cassette resides below the rim of the basin. The plugging solution (e.g. agarose gel) forms a plug within the bottom of the cassette to contain the separation medium.

The invention relates to a transdermal analyte monitoring system comprising a medium adapted to interface with a biological membrane and to receive an analyte from the biological membrane and an electrode assembly comprising a plurality of electrodes, wherein the medium is adapted to react continuously with the analyte, an electrical signal is detected by the electrode assembly, and the electrical signal correlates to an analyte value. The analyte value may be the flux of the analyte through the biological membrane or the concentration of the analyte in a body fluid of a subject. The medium may comprise a vinyl acetate based hydrogel, an agarose based hydrogel, or a polyethylene glycol diacrylate (PEG-DA) based hydrogel, for example. The surface region of the electrode may comprise pure platinum. The system may include an interference filter located between the biological membrane and the electrode assembly for reducing interference in the system. The system may comprise a processor programmed to implement an error correction method that corrects for sensor drift.

The present invention provides an agarose gel separation medium which is uniform in size, controllable and has high hydrophility and high porousness, and can be used for making separation and purification of biological active material. Its preparation method is characterized by that it adopts the following steps: (1) dissolving a certain quantity of agarose in a certain quantity of water under the heating condition to obtain agarose aqueous solution with a certain concentration, using pressure to make said aqueous phase by passed through microporous membrane and make it feed into oil phase to obtain emulsion drop with uniform size; and (2). transferringsaid emulsion into cooling device, under the condition of adopting programmed temperature-falling and slow-mechanically stirring process cooling to below 15 deg.C and making gel be solidified so as to obtain the invented agarose gel microspheres with uniform size.

An alkanethiol monolayer membrane is formed on a substrate having an Au layer using Au-S bonding, and a second layer constructed by a monolayer lipid membrane is formed on its surface to form a BLM. A gel layer is formed using agarose on the side of the BLM having the second monolayer lipid layer. In addition, a polymer layer is formed using the amino acid poly-L-lysine between the surface of the monolayer lipid membrane and the gel layer to immobilize the BLM. The use of this supporting constitution for holding and immobilizing the membrane extends the life-span of the BLM, while the gel layer keeps the electrolyte solution required for maintaining flexibility of the membrane and activating protein on the surface of the BLM. In addition, a BLM having a life-span of one month or more is obtained by arranging a membrane supporting portion that minimizes lifting of the membrane molecules due to buoyancy above the BLM arranged upright in an aqueous system.

The invention provides methods and compositions for azide tagging of biomolecules. In one embodiment of the invention, proteins are tagged by metabolic incorporation of prenylated azido-analog substrates. Examples of such analogs are azido farnesyl diphosphate and azido farnesyl alcohol. The azido moiety in the resulting modified proteins provides an affinity tag, which can be chemoselectively captured by an azide-specific conjugation reaction, such as the Staudinger reaction, using a phosphine capture reagent. When the capture agent is biotinylated, the resulting conjugates can be detected and affinity-purified by streptavidin-linked- HRP and streptavidin-conjugated agarose beads, respectively. The invention allows detection and isolation of proteins with high yield, high specificity, and low contamination without harsh treatment of proteins.

Biological agents such as secretory cells are encapsulated in a hydrophilic gel made of agarose or collagen-agarose and gelatin sponge-agarose combinations. In a preferred embodiment, semi-solid beads are formed from a suspension containing collagen, agarose and secretory cells such as pancreatic islets, the collagen is polymerized to form solid, agarose-collagen beads and the solid beads are coated with agarose. Coating is preferably by rolling the solid beads in about 5-10% agarose, contacting the rolled beads with mineral oil and washing oil from the beads. Beads containing secretory cells can be transplanted into a mammal to treat a condition caused by impaired secretory cell function.

The invention discloses a microcapsule wrapping a solid sun-screening agent, a preparation method and application thereof. The microcapsule comprises the solid sun-screening agent and a natural polymer. The solid sun-screening agent is wrapped in the microcapsule formed by the natural polymer. The solid sun-screening agent is an inorganic ultraviolet screener, an organic ultraviolet adsorbent or a mixture of the inorganic ultraviolet screener and the organic ultraviolet adsorbent. The natural polymer is agar, agarose, cellulose, chitosan or a derivative thereof. The microcapsule provided by the invention can be used in cosmetic to effectively prevent the solid sun-screening agent from being decomposed, and the sun-screening agent is separated from skin, so that irritation to skin is reduced. Hydrophilic natural polymer can cover color, improve feel and enhance cooperation in cosmetic.

The invention discloses a preparation method of agarose immune magnetic microspheres and applications thereof, and relates to a preparation method of microspheres and applications thereof, which solves the technical problems that an existing purified monoclonal antibody is complex in process, is low in yield, and is high in cost, and coupling agents are hypertoxic. The preparation method comprises the following steps of: 1. preparing a ferric oleate precurser; 2. preparing a magnetic fluid; 3. preparing an oil phase and a water phase; 4. preparing agarose magnetic microspheres; 5. preparing activated and crosslinked agarose magnetic microspheres; 6. preparing agarose magnetic microspheres joined with spacer arms; and 7. preparing agarose immune magnetic microspheres. The agarose immune magnetic microspheres provided by the invention are used for purifying genetic engineering recombination trastuzumab monoclonal antibodies. The agarose immune magnetic microspheres prepared by the invention are used for the field of separating and purifying the monoclonal antibodies.

The present invention is a surface-modified base matrix comprised of a porous polymeric base matrix onto which branched hydrophilic polyhydroxy-functional polymers have been covalently attached, wherein the polyhydroxy-functional polymers are hyperbranched polymers presenting a degree of branching (DB) of at least about 0.2 and each polymer is tethered to the base matrix at two or more points. The present matrix can for example be a cross-linked carbohydrate material, such as agarose, and the hyperbranched hydrophilic polymer can e.g. be a copolymer of epichlorohydrin and a sugar. The invention also relates to a method of surface-modification of a porous base matrix by activating functional hydroxy groups thereon and contacting the activated matrix with a hydrophilic hyperbranched hydroxy-functional polymer.

The invention relates to an endotoxin adsorbent, which in detail relates to a method of preparing endotoxin adsorbent for blood perfusion. The method employs spherical agarose gel with good biocompatibility as base material, employs epichlorohydrin, hexamethylene diamine, 1, 1'-carbonyldiimidazole as activating agent, and bonds polymyxin B for endotoxin removal. The invention is characterized in that it overcomes the toxicity of cyanogen bromide and instability of aglycone, improves the safty and reliability of operation, reducesnon-specific adsorption through bonding polymyxin B with 1, 1'-carbonyldiimidazole, and improves biocompatibility of adsorbent and suits for treating endotoxemia. The invention is also characterized by simple experimental procedure and high clearance for endotoxin.

A system and method for mimicking a human brain for MR imaging at various magnetic field strengths is disclosed. A phantom is constructed of a structure having a number of sections. A first section contains a mixture of nickel chloride, agarose gel powder, potassium sorbate, deuterium oxide, and water such that the T1, T2, and proton density values of the first section mimic white matter of the human brain. A second section contains different amounts of the same components such that the T1, T2, and proton density values of the second section mimic gray matter of the human brain. As such, when the phantom is scanned in an MR imaging machine, an optimized flip angle for T1-weighted imaging to improve contrast between white matter and gray matter of the human brain that takes into account proton density differences therebetween can be determined.

A process for preparing high-capacity microreticular agarose gel medium includes such steps as dispersing the granular calcium carbonate as hole-forming agent in the aqueous solution of agarose to obtain suspension, adding it to salad oil, adding emulsifier Span80, emulsifying while stirring, quick cooling for sphericizing, adding epoxy chloropropane, cross linking, reducing with sodium borohydride, and using diluted hydrochloric acid to remove granular calcium carbonate.

The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving / gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and / or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores. A similar process with either agarose beads (made by this or another process) or cored agarose (made by this or another process) can be used to add multiple layers of agarose on to the existing beads. An apparatus for running the process is also disclosed.

The invention relates to a transdermal analyte monitoring system comprising a medium adapted to interface with a biological membrane and to receive an analyte from the biological membrane and an electrode assembly comprising a plurality of electrodes, wherein the medium is adapted to react continuously with the analyte, an electrical signal is detected by the electrode assembly, and the electrical signal correlates to an analyte value. The analyte value may be the flux of the analyte through the biological membrane or the concentration of the analyte in a body fluid of a subject. The medium may comprise a vinyl acetate based hydrogel, an agarose based hydrogel, or a polyethylene glycol diacrylate (PEG-DA) based hydrogel, for example. The surface region of the electrode may comprise pure platinum. The system may include an interference filter located between the biological membrane and the electrode assembly for reducing interference in the system. The system may comprise a processor programmed to implement an error correction method that corrects for sensor drift.

A method for the indirect extraction of DNA greater than 300 kb in size from non-cultivatable organisms contained in an environmental sample is disclosed. The method comprises isolating the organisms from the sample and embedding the isolated organisms in a block of agarose where the organisms are subsequently lysed and the DNA subjected to a first alternating field electrophoresis to extract DNA fragments which are large in size and separate them from the cell debris. The first electrophoretic migration is followed by an enzymatic restriction step and additional electrophoretic migrations. The invention also encompasses DNA obtained by the method.

The invention belongs to the field of pharmacogenomics and genetic diagnosis, and relates to a method used for detecting HLA-B*5801 alleles. The method comprises following steps: a DNA sample to be detected is taken, three pairs of specific primers and a pair of internal primers are taken, amplification of DNA segments is realized by using sequence specific primer method, and then the results of the amplification are analyzed by agarose gel electrophoresis; or sample DNA is extracted, a pair of specific primers, a pair of internal primers and three fluorescence probes are taken, amplification of DNA segments is realized by Taqman probe method using a fluorescence ration PCR instrument, and then the amplification curve is analyzed so as to obtain results. Results analysis methods such as agarose gel electrophoresis, high resolution melting curve and SYBRGreen fluorogenic quantitative PCR are employed in the method. The method has advantages of speediness, convenience, flexibility, high resolution and no contamination; is suitable for detection of HLA-B*5801 alleles in samples such as peripheral blood and hair; and can be used for determining the probability of severe skin adverse reaction of patients with gout or hyperuricemia caused by taking of allopurinol.

The present invention relates to a PCR-RFLP identification method for 7 kinds of Chinese south inshore oysters. Said identification method includes the following several main steps: making genome DNA extraction, PCR amplification of object fragment, selecting proper incision enzyme combination, utilizing selected incision enzyme to enzyme-cut the PCR product and utilizing agarose gel electrophoresis to make detection.

The microflow control chip for protein analysis features that it consists of four basic units connected successively with the sample outlet of the upper basic unit being connected to the sample inletof the next basic unit. They are the first protein isoelectric focusing separation unit; the second protein screening electrophoresis unit with organic polymer selected from cellulose and agarose asscreening medium; the third protein proteinase enzymolyzing unit with specific proteinase bonded to the inner wall or specific proteinase stuffing filled; and the fourth interface unit to convey the enzymolysis product of protein to mass spectral analysis. Using the chip of the present invention in protein analysis can obtain great amount of information in short time and through simple operation.

The invention discloses a rigid ceramic / agarose composite microsphere and a preparation method thereof. The composite microsphere has a uniform composite structure of a rigid ceramic skeleton and agarose gel. The preparation method comprises the following steps of: firstly, preparing ceramic slurries, and uniformly mixing nano-silica powder, zirconia powder or titanium dioxide powder with sodium alginate solution; secondly, preparing a porous ceramic microsphere, inverse suspension dispersing the ceramic slurries in an oil phase, dropping calcium chloride solution to for curing into microspheres, collecting the microspheres, drying, forming the microspheres to through high temperature sintering; thirdly, filling agarose, immersing the ceramic microspheres into agarose solution, and cooling and solidifying after preserving the temperature in a sterilizing pot for a period of time; and fourthly, grinding and screening: grinding a solide mixture of ceramic and the agarose in a mortar, obtaining the microspheres with the required particle size through screening in water, and removing the agarose on the surfaces of the microspheres. The microsphere has the advantages of good rigidity, good controllable density, good hydrophilicity, good sphericity and low cost. Shown by test experiments, the microsphere can be used as a matrix of biomacromolecule chromatographic separation medium.

The invention relates to a polypeptide immunoassay kit, which comprises a protein G or protein A agarose serving as a solid phase carrier, a purified serum polypeptide antibody and PBS buffer solution. The invention also relates to a polypeptide immunomic spectrometry analysis method, which comprises the following steps: coupling a purified serum antibody with a proper solid carrier; allowing the coupled product to bond with a polypeptide marker antigen specifically, and separating the antigen from the carrier by using eluent; and finally, detecting polypeptide antigen in the eluent by high-pass matrix-assisted laser desorption / ionization time of flight mass spectrometry (MALDI-TOFMS). Thus, the complete immunomic spectrometry analysis method is established.

The invention relates to a preparation method and a product of macroporous agarose microspheres. The preparation method is characterized by comprising the following steps: adding a high-concentration surface active agent to an agarose aqueous solution to form a large number of micelles in a water phase; dispersing the water phase into an oil phase; and then preparing the macroporous agarose microspheres according to the characteristic that the micelles of the surface active agent in the water phase can absorb the oil and then swells. The prepared product remains the original mesh shaped gel pores of the agarose gel and also has macropores at hundred-nanometer level (100 to 500nm). The preparation method of the macroporous agarose microspheres, provided by the invention, is simple, and the aperture of the product can be controlled; the prepared macroporous agarose microspheres remain the original excellent performances of the agarose gel and greatly increases the aperture, and are ideal liquid phase chromatogram stationary phase medias and excellent immobilized enzyme carriers; and the macroporous agarose microspheres serving as the liquid phase chromatogram media especially can run at higher flow rate, and can realize quick and efficient separation of biomolecule, in particular biomacromolecule.

The invention discloses an endotoxin adsorbent using a molecular cluster as a functional group and a preparation method thereof, and belongs to the fields of bioseparation and biomedical engineering. By taking agarose gel as a vector, the endotoxin adsorbent is subjected to epoxy activation to be directly coupled with a molecular sieve frame epsilon-polylysine or polyaspartic acid, then the coupled mixture is ingrafted with micromolecular lysine or lycine by virtue of a condensation reaction to prepare the adsorbent. The adsorbent prepared by the method has higher removal rate to endotoxin inserum of a human, but adsorbs less serum protein. Meanwhile, the preparation process of the adsorbent has the advantages of mild conditions, fewer synthesis steps and relatively low material cost.

The invention discloses a DNA (deoxyribonucleic acid) barcoding based molecular identification method and CO I sequence of a leech (whitmania pigra). Polymerase chain reaction (PCR) is carried out by using the method to amplify a CO I gene of the leech, a PCR product is sent to a sequencing company to be sequenced after being verified by agarose gel, the sequencing results are subjected to manual proofreading and sequence splicing and are compared with public sequences, and the tissue to be tested can be judged to be the source of whitmania pigra if the homology of the sequencing results and a gene sequence SEQ ID NO.1 is above 99%. The whitmania pigra variety is quickly and accurately identified by adopting the reliable DNA molecular identification technology, thus improving the accuracy and the reliability and ensuring the safety of the leech as a medicine.