896 results about "Molecular identification" patented technology

The invention discloses a cultivating method of morchella importuna. The cultivating method of the morchella importuna comprises a bacterium culturing producing step, a cultivation material producing step, a hydration processing step, a managing and protecting step and the like. A maternal species (HKAS 62868) which can be identified as the morchella importuna (the number of the Genbank is JQ 618576) through a molecular identification can be used for cultivating cultispecieses. After the hydration processing is carried out on the cultispecieses, the cultispecieses are sowed on the face of the right land and then are earthed up. The cultivation materials can be produced and added in the field and the field can be normally managed for 80-120 days, so that sporocarps can be obtained. The cultivating method of the morchella importuna aims at providing an edible mushroom cultivating method which not only is beneficial to the sustainable development of ecology, but also helps to increase farmer income and a novel cultivating bacterial strain is provided for the industrialization development of the morchella importuna at the same time.

The invention discloses a preparation method of a temperature sensitive adsorbent of halloysite magnetic composite material surface blotting, belonging to the technical field of environment functional material preparation. The preparation method comprises the following steps: preparing a ferroferric oxide / halloysite nanotube (Fe3O4 / HNTs) magnetic composite material through a simple and effective solvent thermosynthesis method; and then, performing ethylene modification on the magnetic composite material; and preparing the temperature sensitive adsorbent of halloysite nanotube magnetic composite material surface blotting through a radical polymerization process, and utilizing the adsorbent in selective identification and separation of 2,4,5-trichlorophenol in aqueous solution. The prepared temperature sensitive blotting adsorbent has excellent thermal and magnetic stability, sensitive magnetic and thermal induction effect, high adsorption capacity, excellent reversible adsorption / release function with temperature and obvious TCP molecular identification performance.

The invention discloses a molecular identification method for lasiohelea taiwana. The method is characterized in that two different genes, namely a COI gene and an ITS2 gene are adopted to be combined, and the lasiohelea taiwana is subjected to species identification by comparison of the similarity of the gene sequences. By adopting the molecular identification method for lasiohelea taiwana provided by the invention, accurate and rapid identification of the lasiohelea taiwana is facilitated. A basis is provided for molecular identification of the lasiohelea taiwana. The new variety can play a certain role in transmission of plant pollen and environment harmless reaction.

The invention discloses a molecular identification method for a DNA bar code of lumbricus and a CO I sequence thereof. The method comprises the following steps: amplifying a sample CO I gene through polymerase chain reaction (PCR); verifying the PCR product through agarose gel and then sending to a biological sequencing company for sequencing; manually correcting and splicing sequence for the sequencing result; comparing with a public sequence; and if the homology of the gene sequence SEQ ID NO.1-NO.4 is above 99%, confirming a to-be-detected tissue as a source of pheretima aspergillum or Shanghai lumbricus. According to the invention, a reliable DNA molecular identification technique is adopted; the morphological identification for lumbricus varieties is not required; the lumbricus varieties and non-drug lumbricus varieties specified in Chinese Pharmacopoeia can be quickly and accurately identified; besides, the pheretima aspergillum and the Shanghai lumbricus can be further distinguished; the accuracy and the reliability can be enhanced; the safety of the lumbricus used as a medicinal material can be guaranteed.

The invention belongs to the field of food, in particular belongs to the field of pickles in the food industry and in particular provides a microbial fermentation agent capable of being used for continuously fermenting pickles in multiple batches. Main microbes in Sichuan old saline water pickles are determined to be lactobacilluspentosus, lactobacillus brevis and kazachstaniae xigua through study by various advanced means, such as traditional separation and purification, molecular identification, high throughput measurement and quantitative PCR, and have a content ratio of 10 to 1 to 1. 1 / 10000-5 / 10000 of the microbes are added when the pickles are made. The microbial fermentation agent has the positive effects that compared with pickle fermentation agents (single strains) of the same kind, the microbial fermentation agent has the closer fermentation flavor to natural fermentation; and a stable fermentation system (equivalent to the pickle mother water) can be formed only by adding the fermentation agent when the pickles are made for the first time and can be used for continuously making at least 20 batches of pickles.

The invention discloses a method for molecular identification of Siniperca chuatsi basilewsky and Siniperca scherzeri Steindachner and a kit used by the same. The method for molecular identification comprises the following steps: DNA extraction, DNA fragment amplification, electrophoresis of PCR products and identification by a silver-staining method. The kit contains a pair of SSR primers and a standard map. The method for molecular identification can improve the efficiency and accuracy of identification of Siniperca chuatsi basilewsky and Siniperca scherzeri Steindachner, and is expected to solve the identification problem in the aquiculture of Siniperca chuatsi basilewsky and Siniperca scherzeri Steindachner and stimulate the sound development of Siniperca chuatsi culture industry.

The invention discloses amino acid sequences of GAI proteins of Pyrus betulaefolia and Pyrus bretschneideri, and nucleotide sequences for coding the GAI proteins, wherein the sequences are disclosed as SEQ ID NO1-4 in the sequence table. The invention also discloses a molecular identification method based on transfer of GAI mRNA between pear rootstock and scion, which comprises the following steps: (1) micrografting seedlings in test tubes by using Pyrus bretschneideri as scion and using Pyrus betulaefolia as rootstock; (2) looking for the respective specific enzyme-digestion sites of the GAI genes of the Pyrus bretschneideri and the Pyrus betulaefolia, and respectively designing a primer at the upstream and downstream of the enzyme-digestion site; (3) and carrying out enzyme-digestion on the Pyrus betulaefolia, the Pyrus bretschneideri, and the RT-PCR product of the Pyrus betulaefolia and the Pyrus bretschneideri after grafting, and comparing the enzyme-digestion spectra before and after the grafting to identify the transfer conditions. The method is quick, sensitive, accurate, simple and convenient, and is applicable to other pear plants.

The invention discloses a fluorescence nanocrystal quartz fluorescent sensor for molecular identification-based multicomponent simultaneous saccharide detection and a method for detecting saccharides by using the same. The invention provides a preparation method of a molecular identification-based fluorescence nanocrystal comb quartz plate, which comprises the following steps: selecting an identifier corresponding to the saccharides; preparing a fluorescence nanocrystal, and carrying out surface modification on the fluorescence nanocrystal according to the literature; and modifying the modified fluorescence nanocrystal onto surfaces of different probes of the comb quartz plate by using layer-by-layer accumulation surface modification technique. The invention also provides a method for multicomponent simultaneous saccharide detection, which comprises the following steps: immersing the modified quartz plate into a food sample solution which is simply pulpified, installing the quartz plate onto a sealed silica dish, and detecting the saccharides in the sample. The invention has the advantages of strong specificity, high sensitivity, short detection time and low cost. The saccharide fluorescence detection method has a quick and simple operation process, and the reaction and the result are automatically completed and recorded by instruments.

The invention discloses a method for quickly extracting fungal DNA for PCR amplification, which comprises the steps of: (1) culturing fungi to obtain fungal mycelia; (2) placing the fungal myceliua into a centrifugal tube filled with a lysate; (3) alternately freezing and thawing the centrifugal tube; and (4) centrifuging the thawed fungal mycelia, taking a supernatant, adding 95 percent ethanol into the supernatant, mixing the mixture evenly, quickly freezing the mixture by liquid nitrogen, centrifuging the mixture, removing a supernatant, and air-drying a precipitate to obtain the fungal DNA. The method has simple operation, is time-saving and labor-saving, reduces the loss of the fungal DNA during the grinding, does not need phenol and chloroform for extraction, and avoids the contact of a toxic reagent. The DNA extracted by the method has good integrity, high purity and high yield, can be directly used for a PCR amplification reaction, and can efficiently amplify a target product for cloning and sequencing. The method improves the extraction efficiency of the fungal DNA, reduces the cost for the extraction of the fungal DNA, and can effectively improve the molecular identification and detection efficiency of the fungi.