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600 results about "Sterility" patented technology
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Sterility is the physiological inability to effect sexual reproduction in a living thing, members of whose kind have been produced sexually. Sterility has a wide range of causes. It may be an inherited trait, as in the mule; or it may be acquired from the environment, for example through physical injury or disease, or by exposure to radiation.
Multi-lancet device with sterility cap repositioning mechanism
ActiveUS20050149089A1Improve convenienceEasy to disassembleIntravenous devicesSensorsEngineeringLancet devices
A lancing device has a carousel of lancets removably received in a housing. In exemplary embodiments, the lancing device includes a sterility cap positioning mechanism that removes sterility caps from the lancets for actuation and afterwards replaces the sterility caps onto the lancets. Preferably, the lancing device includes a lancet advancing mechanism that automatically advances sequential lancets of the lancet carousel for charging and actuating, and a drive mechanism for actuating the lancets. Also provided are replacement lancet carousels, which include a cylindrical carrier with axial openings and a plurality of the lancets axially oriented in the openings.
Owner:FACET TECH LLC
Methods for producing hybrid seed
InactiveUS20070199095A1Suppress expression of proteinFemale sterilityOther foreign material introduction processesFermentationRecombinant DNAHybrid seed
This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.
Owner:MONSANTO TECH LLC
Nucleotide sequences mediating male fertility and method of using same
InactiveUS20060288440A1Microbiological testing/measurementOther foreign material introduction processesAdemetionineGamete
Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants. In one such method, the homozygous recessive condition of male sterility causing alleles is maintained after crossing with a second plant, where the second plant contains a restoring transgene construct having a nucleotide sequence which reverses the homozygous condition. The restoring sequence is linked with a hemizygous sequence encoding a product inhibiting formation or function of male gametes. The maintainer plant produces only viable male gametes which do not contain the restoring transgene construct. Increase of the maintainer plant is also provided by self-fertilization, and selection for seed or plants which contain the construct.
Owner:PIONEER HI BRED INT INC
Methods for cleaning luer connectors
InactiveUS8197749B2Improves microbial killReduces and prevents evaporative lossDiagnosticsSurgeryAnatomySterility
Illustrative pairs of caps are disclosed, each of the caps being sized and shaped to provide a protective union about a separated medical connector. A pair of caps can include a male cap and a female cap, each of which is configured to be coupled to the other cap in an assembly. The assembly is sealed until the caps are separated for use, thereby maintaining sterility of the internal surfaces of the caps. Related methods are also disclosed.
Owner:MERIT MEDICAL SYST INC
Application of male sterility gene OsDPW2 and rice sterility recovery method
ActiveCN106011167AIncrease productivityReduce labor costsVectorsPlant peptidesGenetic engineeringJaponica rice
The invention discloses application of male sterility gene OsDPW2 and a rice sterility recovery method. OsDPW2 gene is knocked out, changed or inhibited by using the conventional genetic engineering method or on the basis of a CRISPR / Cas9 system, and the expression level of the OsDPW2 gene is reduced in japonica rice background wild type rice, so that a rice male sterility line is obtained. The invention further relates to the rice sterility recovery method, the OsDPW2 gene is amplified by primers, a mutant plant is transformed by a genetic transformation means, and a mutant can be recovered to be in wild phenotype. In a rice nutritional growth period of the rice male sterility line prepared by the invention, abnormal phenotypes do not exist; but in an anther development process of a reproductive growth period, abnormal phenotypes exist. If the male sterility gene OsDPW2 is used in cross breeding, operation of female parent emasculation can be avoided, production efficiency is greatly improved, labor cost is reduced, and the male sterility gene OsDPW2 has important application on agricultural production.
Owner:SHANGHAI JIAO TONG UNIV
Site-directed knockout system for rice TMS10 gene, and applications thereof
InactiveCN107326042AExhibit male sterilityEfficient breedingTransferasesNucleic acid vectorBiotechnologyGermplasm
The present invention relates to a site-directed knockout system for a rice TMS10 gene, and applications thereof. The site-directed knockdown system comprises a CRISPR / Cas9 system and a sgRNA target, wherein the sgRNA target is the sequence containing PAM or NGG in a rice male sterile gene TMS10, the CRISPR / Cas9 system is CC-TMS10-1, and the CC-TMS10-1 target sequence is a SEQ ID NO.1 sequence from site 2281 to site 2299. The applications comprise carrying out targeted knockout on the TMS10 genes of different japonica rice varieties and different Indica rice varieties by separately using CC-TMS10-1, wherein the experiment results prove that the heterozygous mutation transformation plant produced through the site-directed knockout induction in different rice varieties shows the temperature sensitive male sterility characteristic. According to the present invention, the site-directed knockout system provides the efficient breeding method for the creation of the temperature sensitive male sterile line germplasm resources based on the rice temperature-sensitive male sterile gene TMS10 and the rice hybridization breeding.
Owner:SHANGHAI JIAO TONG UNIV
Sterilization wraps and methods for sterilizing articles
InactiveUS20050079093A1Easy to useLess-costly to manufactureSurgical furnitureSamplingAbsorbent materialSterility
There is provided an improved sterilization wrap and a method for using the improved sterilization wrap to sterilize an article. The wrap is made of at least one sheet, and preferably two sheets, of sterilization material and in some embodiments, includes an additional sheet which may be made of an absorbent material. The sheet of absorbent material may have a smaller perimeter than at least one sheet of sterilization material. One side of at least one sheet of sterilization material includes a central portion. The absorbent material may be attached to the central portion of at least one sheet of sterilization material. The article to be sterilized is placed on the sterilization wrap and is then wrapped. The absorbent material provides sterility protection for the article in addition to the at least one sheet of sterilization material and also wicks moisture away from the article after sterilization has taken place. A visual indicator may be provided to indicate whether or not the article has been exposed to adequate sterilization conditions.
Owner:ALLEGIANCE CORP
Temperature sensing male fertile gene and use thereof
ActiveCN101333533AImprove selection efficiencyBacteriaMicrobiological testing/measurementBiotechnologyMicroorganism
The invention discloses a thermo-sensitive male fertility gene and an application thereof and also discloses a genetic marker of the thermo-sensitive male fertility gene, and provides a protein coded by the thermo-sensitive male fertility gene and a carrier containing the thermo-sensitive male fertility gene and a recombinant microorganism. The invention also discloses the application of the thermo-sensitive male fertility gene in cultivating a thermo-sensitive male sterile line and an application of the genetic marker in rice breeding. The thermo-sensitive fertility gene TSSNR is acquired through the map-based cloning technique of Annong S-1 thermo-sensitive sterility gene locus tms5, functions of the gene are validated through transgene experiments, and the transgene technique of RNAi or antisense RNA or the over-expression dominant negative principle is provided, functions of the TSSRZ gene in normal rice varieties are incapable, so as to cultivate the thermo-sensitive male sterile line, and the application value is significant.
Owner:SOUTH CHINA AGRI UNIV
Bioreactor for organ reconstruction and augmentation
InactiveUS20070275363A1Inhibit cell growthBioreactor/fermenter combinationsBiological substance pretreatmentsSufficient timeUrothelial Cell
Bioreactors are used in neo-organ production to allow for an appropriate environment for the maintenance of healthy culturing conditions from pre-wetting to shipment of the neo-organ. The closed system “all-in-one bioreactor” is designed to allow for minimal exposure of the scaffold to the open air in order to maintain sterility. The design allows for the same container to be utilized for sterilization, pre-wetting, cell seeding, medium exchange, and shipment. The “all-in-one” bioreactor also remains completely closed after the urothelial cell seeding step to the implantation at the clinical site. This allows for sufficient time for release testing to occur so the neo-organ can be implanted into the patient.
Owner:TENGION
Device and Method for Generating an Aerosol From a Liquid Formulation and Ensuring Its Sterility
A drug delivery device containing a sterile multi dose reservoir. Said sterile reservoir can be used with many types of delivery including injectors or aerosol drug delivery systems. Elevated pressure surrounding the reservoir is used during storage to ensure sterility is maintained. Mechanisms to prevent delivery in the case of potential compromise of sterility are disclosed. A device using the pressure to meter formulation from the reservoir is disclosed.
Owner:ARADIGM
Breeding method of polyclonal antibody cold-resistant two-line sterile line
ActiveCN103229712ALow costReduce pollutionPlant genotype modificationAnimal scienceTryporyza incertulas
The invention discloses a breeding method of a polyclonal antibody cold-resistant two-line sterile line, belonging to the technical field of breeding for disease resistance. According to the breeding method, by using a two-line sterile line as a recurrent parent, and using a tryporyza incertulas-resistant variety, a rice blast-resistant variety, a stunt-resistant variety and a cold-resistant variety as antigen parents, through a hybrid polymerization process, antigen genes of four rice diseases, insect and cold damages are polymerized in the same rice material, and then subjected to multi-generation backcross and screening together with the recurrent parent of the two-line sterile line, so that the insect and cold-resistant two-line sterile line, with economical characters, sterility period translative temperature and sterility highly consistent with those of the recurrent parent of the two-line sterile line, is bred. The two-line sterile line is used for preparing two-line hybrid rice, and thus the level of resisting insects and low-temperature coldness of the hybrid rice can be improved.
Owner:福建亚丰种业有限公司
Adjuvant immune therapy in the treatment of solid tumors through modulation of signaling pathways following engagement of humoral and cell mediated responses
InactiveUS20020187130A1Evaluating efficacyAccurate assessmentBiocideSaccharide peptide ingredientsAdjuvantWhite blood cell
Owner:KINDNESS GEORGE +2
Method for creating rice engineering maintainer lines preventive against gene flow and application of rice engineering maintainer lines
InactiveCN105063083AAvoid Ecological Security RisksAvoid Ecological PollutionFermentationVector-based foreign material introductionAgricultural scienceMutant line
The invention discloses a method for creating rice engineering maintainer lines preventive against gene flow. The method includes the steps of 1, site-specifically inserting an exogenous reporter gene and a pollen lethal gene into a flanking sequence of a fertility control gene of a wild rice chromosome so as to obtain a transgenic line containing the exogenous reporter gene and the pollen lethal gene, wherein the exogenous reporter gene, the pollen lethal gene and the fertility control gene are able to closely link and inherit; 2, hybridizing wild rice and homozygous recessive rice common genic male sterile lines to obtain F1-generation plants, hybridizing the F1-generation plants as male parents to the transgenic line to obtain a generation F2, screening the generation F2 to obtain hybrid lines containing the exogenous reporter gene, namely rice engineering maintainer lines required, wherein the homozygous recessive rice common genic male sterile lines are mutant lines with the sterility control gene. The invention further discloses application of the rice engineering maintainer lines preventive against gene flow in rice breeding.
Owner:HUNAN HYBRID RICE RES CENT
Stable rocuronium injection preparation and preparation method thereof
InactiveCN103462885AOrganic active ingredientsInorganic non-active ingredientsMuscle relaxationSterility
The invention discloses a stable rocuronium injection preparation prescription and a preparation method thereof. The injection contains the active ingredient rocuronium, a stabilizer, an osmotic pressure regulator, a pH (potential of Hydrogen) regulator and injection water, and has two specifications, i.e., 2.5 ml:25 mg and 5ml:50 mg. Rocuronium is added into the proper stabilizer, so that the problem of stability is solved; autoclave sterilization of 121 DEG C is adopted with the help of a filtration sterilization mode to guarantee the sterility. The preparation has the advantages of reasonable prescription, simple process and stable quality and is suitable for industrial production. The injection is used for muscular relaxation of surgical patients.
Owner:河北凯盛医药科技有限公司
Application of paddy rice temperature sensitive male sterility gene TMS10 and fertility restoration method
ActiveCN105821074AControl reproductive processPlant peptidesFermentationGrowth phaseAgricultural science
The invention relates to an application of a paddy rice temperature sensitive male sterility gene TMS10 and a fertility restoration method. The amino acid sequence of TMS10 is represented by SEQ ID No.1. A conventional method is adopted to knock, change or inhibit TMS10 gene to reduce the expression level of TMS10 gene in common paddy rice species so as to obtain paddy rice male sterility line. The prepared paddy rice sterility line does not have abnormal phenotype during the vegetative growth phase; anther development is abnormal during the reproductive stage; the expressions of the phenotype are the same in the mutants of non-glutinous rice and japonica rice; the paddy rice is sterile at an average temperature of 28 DEG C, and paddy rice is fertile at an average temperature of 22 DEG C. The temperature sensitive characteristic, which is utilized during the process of two line hybrid seed production, is widely used in agricultural production.
Owner:SHANGHAI JIAO TONG UNIV
New hybrid system for brassica napus
InactiveUS20100222605A1Restore fertilityGenetic engineeringFatty-oils/fats productionBrassicaHybrid system
This invention relates to a nuclear conditional male sterility system in Brassica napus. Embodiments of the invention provide for the (male sterile) prebasic female (MsMsrfrf), the (male fertile) maintainer line (msmsrfrf), the (male sterile) basic female line (Msmsrfrf), and hybrid lines. Further provided are methods for the production of those lines. Further embodiments of the invention relate to markers associated to the sterility, fertility and maintainer alleles and the use of those markers in providing a hybrid system.
Owner:SYNGENTA PARTICIPATIONS AG
Application of male sterility gene OsDPW3 and rice fertility restoration method
ActiveCN108130328AControl reproductive processIncrease productivityPlant peptidesFermentationRestoration methodWild type
The invention discloses application of a male sterility gene OsDPW3 and a rice fertility restoration method. The gene OsDPW3 is knocked out, changed or inhibited by using the conventional genetic engineering method or based on a CRISPR / Cas9 system, and the OsDPW3 gene expression level is decreased in wild type rice with japonica rice background, so that a rice male sterile line is further obtained. The invention also relates to the rice fertility restoration method. According to the method, the OsDPW3 gene is amplified by means of primers, and mutant plants are transformed by using a genetic transformation means, so that the mutant can be restored to a wild-type phenotype. The rice male sterility line prepared by the method has no abnormal phenotype in the rice vegetative growth period, but abnormality occurs in an anther development process of the reproductive growth period. If the rice male sterility line is applied to cross breeding, the work of castration of a female parent can beeliminated, so that the production efficiency is greatly improved, and the labor cost is lowered. The male sterility gene OsDPW3 has important application to agricultural production.
Owner:SHANGHAI JIAO TONG UNIV
Molecular marker for paddy recessive genic male sterility gene cyp704b2 and application thereof
ActiveCN106676179AStrong specificityMeet the need for assisted selectionMicrobiological testing/measurementDNA/RNA fragmentationForward primerElectrophoresis
The invention provides a molecular marker for a paddy recessive genic male sterility gene cyp704b2 and application thereof, belonging to the technical field of plant biology. The molecular marker provided by the invention comprises two forward primers SEQ ID NO.1 and SEQ ID NO.2 and one reverse primer SEQ ID NO.3. The molecular marker can be utilized to complete the genetic typing of the paddy recessive genic male sterility gene cyp704b2 on the basis of conventional PCR and PAGE glue electrophoresis. The molecular marker has the advantages of simplicity and convenience in operation, high typing speed, accurate result and low cost, is capable of increasing the selection efficiency of the target trait and can meet the requirements of large-scale molecular marker-assisted selection breeding.
Owner:HAINAN BOLIAN RICE GENE TECH CO LTD
Method and apparatus for validation of sterilization process
An apparatus, system and method for verifying the achievement of a desired sterility assurance level (SAL) for components manipulated within a low-energy electron beam sterilization chamber. The components are preferably pre-sterilized and connected together in an assembly fashion which creates and maintains the sterility of the connection by subjecting the components to low-energy (less than 300 KeV) electron beam radiation. The verification is completed by measuring the sterilization dose delivered to a sensor, also known as a dosimeter, positioned within the sterilization process to simulate the components.
Owner:BAXTER INT INC
Universal type breeding method for rice engineering maintainer line, and application thereof in propagation of ordinary nucleic male sterility lines of rice
ActiveCN102870670ABroad recovery spectrumFree combinationMicrobiological testing/measurementVector-based foreign material introductionBiotechnologyColor marker
The invention discloses a universal type breeding method for a rice engineering maintainer line. The method comprises the following steps: establishing a dual-element expression vector of color marker gene C; shifting the vector into a type with the genetype of SS through a transgenic method to obtain transgenic strains; obtaining isozygoty strains through selfing propagation; analyzing the insertion site of gene C of each isozygoty strain and obtaining an engineering maintainer line candidate bank through screening and establishment; and crossbreeding ordinary nucleic male sterility lines of rice with the genetype of SS with the isozygoty strains in the candidate bank to obtain F1, obtaining F2 through selfing propagation of F1, breeding seeds with no color in the F2, inspecting sterile stains in each group of F2 stain after breeding, and screening the seeds of F1, where the sterile stains accounting for more than 98% of the F2 come from to take the seeds as the engineering maintainer line. The breeding method provided by the invention has the advantages of short period, strong universality, high efficiency and the like; and the obtained engineering maintainer line can be applied to mechanized and large-scale propagation of ordinary nucleic male sterility lines of rice.
Owner:HUNAN HYBRID RICE RES CENT
Gonadal function improving agents
ActiveUS20060287227A1Reduce releaseReduces of production of testosteroneOrganic active ingredientsPeptide/protein ingredientsOvulation timesDepressant
Owner:TAKEDA PHARMA CO LTD
Rice CYP704B2 gene mutant, as well as molecule identification method and applications thereof
ActiveCN104894144ABreeding benefitsMicrobiological testing/measurementFermentationGenetically modified riceAgricultural science
The invention provides a rice CYP704B2 gene mutant and applications thereof, belonging to the technical field of genetic engineering. A nonglutinous rice variety 93-11 is subjected to induced mutation by cobalt 60 radiation, so as to cause deletion of two G basic groups behind 1267 basic groups of a rice CYP704B2 gene; the rice CYP704B2 gene mutant is named as cyp704B2-2, and a nucleotide sequence thereof is shown as SEQ ID No.1 and further verifies that the mutant causes rice recessive genic male sterility; the rice CYP704B2 gene mutant can be used for preparing transgenic rice with recessive genic male sterility, and plays an important role in genetic improvement and breeding of rice germplasm resources. The invention also provides a molecular marker identification method of the mutant and applications of the method in breeding and seed production.
Owner:HAINAN BOLIAN RICE GENE TECH CO LTD
Gene MS33 related to maize male nuclear sterility and application thereof in cross breeding
The invention discloses a gene MS33 related to maize male nuclear sterility and application thereof in cross breeding. The invention provides MS33 protein related to maize male nuclear sterility, which is shown in (a) or (b): (a) protein composed of an amino acid sequence shown in a sequence 1 in a sequence table; or (b) protein which is derived from the sequence 1 by substituting and / or deleting and / or adding one or more amino acid residues on the amino acid sequence of the sequence 1, and related to plant male fertility. MS33 gene mutation causes maize complete male sterility, characters are complete, and environmental influence is avoided. The gene has application potential in maize hybrid seeds.
Owner:CHINA AGRI UNIV
Coating carrier and its use in method for sperm maturity detection and non-invasive mature sperm separation
InactiveCN101487841ADeterminable maturity statusPromote maturityBioreactor/fermenter combinationsBiological substance pretreatmentsMale infertilityEmbryo
The invention discloses a coated carrier and the application thereof in the method for detecting maturity of sperms and separating mature sperms noninvasively; the coated carrier is a carrier coated by hyaluronic acid, or hyalurate or hyaluronic acid derivatives; the detecting method is as follows: preparing specimens; loading the specimens to the coated carrier and covering with microscopic glass; reacting for 5-60 minutes at room temperature; observing by a microscope and counting; and calculating the combination ratio of hyaluronic acid of active sperms. The separating method is as follows: preparing specimens; loading the specimens to the coated carrier, reacting for 5-120 minutes at the temperature of 18-37 DEG C; and collecting the combined sperms. The coated carrier has the advantages of precise and reliable results, simple and fast operation, low cost and good safety performance, and can be used for estimating sperms quality, sperms maturity and analysis of the causes of sterility disease of mails, and is applicable to assisting reproduction or reproduction research center to safely collect mature and active sperms, so that the goal of improving embryo quality greatly is achieved.
Owner:SHENZHEN PEOPLES HOSPITAL
System and methods for providing protective coverage of an operational surface
InactiveUS20150096475A1Freshness compromisedSterility compromisedShow cabinetsSurgical furnitureSurgical instrumentationEmergency medicine
The present invention relates generally to a system and methods for providing protective coverage of an operational surface and any items that may be on the surface. Certain embodiments of the system may be used to help to maintain the sterility of a surface and the items on the surface. Advantageously, such embodiments of the system may be used to maintain the sterility of surgical instruments and materials, and the table surface on which they are placed prior to the start of the surgical procedure. Other embodiments of the system may be used to at least help to prevent the contamination of or maintain the freshness of food and the containers and utensils used to hold, prepare, and serve the food.
Owner:LEE SEON KYU +1
Devices and Methods for Sampling Biological Fluids
ActiveUS20090305407A1Easy to pumpReduce pollutionBioreactor/fermenter combinationsBiological substance pretreatmentsEngineeringBiological fluids
Devices, instruments, systems and methods are provided in which a primary line that receives a biological fluid is selectively sampled by a plurality of collection chambers. Selective sampling occurs by selectively accessing the primary line and selective engagement of a sampling pump under control of a microprocessor. Further, the instrument housing reversibly houses a drive assembly and sample collection housing to permit the interchangeability of drive assemblies and collection housings and thus enhance sterility or reduction of cost.
Owner:STRATEGIC APPL
Methods for producing hybrid seed
InactiveCN105325288AAvoid mistakesClimate change adaptationHorticulture methodsBiotechnologyHybrid seed
This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.
Owner:MONSANTO TECH LLC
Sterility cover for medical device surface
An enclosure for a medical instrument including a pair of flexible sheets transmissive to a signal generated by a subject, the pair of sheets being arranged in parallel to each other, sealed along the sides and the top, and between the opposing lateral sides and the top and bottom ends along one or more seam lines.
Owner:MA RICHARD
Combined detection kit for seminal plasma
ActiveCN103760331AComprehensive detection indicatorsThe detection method is simpleMicrobiological testing/measurementBiological testingWhite blood cellInfertility
The invention discloses a combined detection kit for seminal plasma. The nine major indexes of pH value, leukocyte esterase, lecithin body, citric acid, zinc, acid phosphatase, elastase, fructose and neutral alpha-glucosidase in the seminal plasma can be rapidly, accurately, simply, conveniently and practically detected at one time in a combined way, functions of glands such as the prostate, the epididymis and the vesicula seminalis can be comprehensively evaluated, detailed related information is provided for the clinical diagnosis and treatment of caused related diseases such as prostatitis, reproductive system inflammation, sexual dysfunction and sterility infertility, a market gaps are filled, and the combined detection kit is easy to clinically popularize.
Owner:AUTOBIO DIAGNOSTICS CO LTD
Breeding method for pod dehiscence-resistant cytoplasmic sterile line and pod dehiscence-resistant cytoplasmic maintainer line of brassica napus
InactiveCN102342245AImprove stabilityGood reproducibilityPlant genotype modificationDiseaseAgricultural science
The present invention discloses a breeding method for pod dehiscence-resistant cytoplasmic sterile line and pod dehiscence-resistant cytoplasmic maintainer line of brassica napus, and belongs to a cultivation method for a new crop variety. With the method provided by the present invention, the detection method for pod dehiscence resistance of the rape is improved; the pod dehiscence-resistant germplasm resource is screened; the screened pod dehiscence-resistant line H155 and the cytoplasmic sterile line 077A are subjected to hybridization, and continuous 8 backcrosses to breed the cytoplasmic sterile line H155A, wherein the sterility rate of the H155A is 100%. According to the present invention, the H155A has characteristics of micro pollen at the low temperature, complete sterility at the high temperature, good agronomic characteristics, cold resistance, viral disease resistance and sclerotium disease resistance. The erucic acid content and the glucosinolate content in the H155A is consistent with the erucic acid content and the glucosinolate content in the cytoplasmic maintainer line of the H155A. The pod dehiscence-resistant cytoplasmic sterile line and the pod dehiscence-resistant cytoplasmic maintainer line of the brassica napus establish the foundation for breeding the domestic pod dehiscence-resistant cytoplasmic sterile line hybrids.
Owner:HENAN ACAD OF AGRI SCI
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