585 results about "Sterility" patented technology

The present invention relates to a site-directed knockout system for a rice TMS10 gene, and applications thereof. The site-directed knockdown system comprises a CRISPR/Cas9 system and a sgRNA target, wherein the sgRNA target is the sequence containing PAM or NGG in a rice male sterile gene TMS10, the CRISPR/Cas9 system is CC-TMS10-1, and the CC-TMS10-1 target sequence is a SEQ ID NO.1 sequence from site 2281 to site 2299. The applications comprise carrying out targeted knockout on the TMS10 genes of different japonica rice varieties and different Indica rice varieties by separately using CC-TMS10-1, wherein the experiment results prove that the heterozygous mutation transformation plant produced through the site-directed knockout induction in different rice varieties shows the temperature sensitive male sterility characteristic. According to the present invention, the site-directed knockout system provides the efficient breeding method for the creation of the temperature sensitive male sterile line germplasm resources based on the rice temperature-sensitive male sterile gene TMS10 and the rice hybridization breeding.

The invention discloses a thermo-sensitive male fertility gene and an application thereof and also discloses a genetic marker of the thermo-sensitive male fertility gene, and provides a protein coded by the thermo-sensitive male fertility gene and a carrier containing the thermo-sensitive male fertility gene and a recombinant microorganism. The invention also discloses the application of the thermo-sensitive male fertility gene in cultivating a thermo-sensitive male sterile line and an application of the genetic marker in rice breeding. The thermo-sensitive fertility gene TSSNR is acquired through the map-based cloning technique of Annong S-1 thermo-sensitive sterility gene locus tms5, functions of the gene are validated through transgene experiments, and the transgene technique of RNAi or antisense RNA or the over-expression dominant negative principle is provided, functions of the TSSRZ gene in normal rice varieties are incapable, so as to cultivate the thermo-sensitive male sterile line, and the application value is significant.

Bioreactors are used in neo-organ production to allow for an appropriate environment for the maintenance of healthy culturing conditions from pre-wetting to shipment of the neo-organ. The closed system “all-in-one bioreactor” is designed to allow for minimal exposure of the scaffold to the open air in order to maintain sterility. The design allows for the same container to be utilized for sterilization, pre-wetting, cell seeding, medium exchange, and shipment. The “all-in-one” bioreactor also remains completely closed after the urothelial cell seeding step to the implantation at the clinical site. This allows for sufficient time for release testing to occur so the neo-organ can be implanted into the patient.

A drug delivery device containing a sterile multi dose reservoir. Said sterile reservoir can be used with many types of delivery including injectors or aerosol drug delivery systems. Elevated pressure surrounding the reservoir is used during storage to ensure sterility is maintained. Mechanisms to prevent delivery in the case of potential compromise of sterility are disclosed. A device using the pressure to meter formulation from the reservoir is disclosed.

The invention combines a novel combination with two especially important aspects: first, the invention proposes to simultaneously stimulate response in white blood cells and a patient's tumor cells with a mitogen-challenging compound, preferably a lectin, in the preferred mode the selected lectin being phytohemagglutin ("PHA"), and second, to generate heat shock protein. A method of treatment is set out. The method of manufacturing proposed utilizes a system calculated to better insure sterility and streamline production of the cytokine modulator. A method of testing in conjunction with the therapy is also claimed utilizing clinical assessment of disease activity, patient performance status, and quality of life questionnaire. Should efficacy of a treatment fall off, particularly because of mutation or adaption, the composition and method may be re- applied. The invention is not limited to humans, but is also applicable to mammals. The composition is usable as a stand-alone composition, but preferably is used in conjunction with standard therapy such as radiation, chemotherapy or surgery, particularly surgical therapy, and in conjunction with the administration of cystine, as later defined, to enhance immune system competency.

The invention discloses a method for creating rice engineering maintainer lines preventive against gene flow. The method includes the steps of 1, site-specifically inserting an exogenous reporter gene and a pollen lethal gene into a flanking sequence of a fertility control gene of a wild rice chromosome so as to obtain a transgenic line containing the exogenous reporter gene and the pollen lethal gene, wherein the exogenous reporter gene, the pollen lethal gene and the fertility control gene are able to closely link and inherit; 2, hybridizing wild rice and homozygous recessive rice common genic male sterile lines to obtain F1-generation plants, hybridizing the F1-generation plants as male parents to the transgenic line to obtain a generation F2, screening the generation F2 to obtain hybrid lines containing the exogenous reporter gene, namely rice engineering maintainer lines required, wherein the homozygous recessive rice common genic male sterile lines are mutant lines with the sterility control gene. The invention further discloses application of the rice engineering maintainer lines preventive against gene flow in rice breeding.

The invention discloses a stable rocuronium injection preparation prescription and a preparation method thereof. The injection contains the active ingredient rocuronium, a stabilizer, an osmotic pressure regulator, a pH (potential of Hydrogen) regulator and injection water, and has two specifications, i.e., 2.5 ml:25 mg and 5ml:50 mg. Rocuronium is added into the proper stabilizer, so that the problem of stability is solved; autoclave sterilization of 121 DEG C is adopted with the help of a filtration sterilization mode to guarantee the sterility. The preparation has the advantages of reasonable prescription, simple process and stable quality and is suitable for industrial production. The injection is used for muscular relaxation of surgical patients.

The invention relates to an application of a paddy rice temperature sensitive male sterility gene TMS10 and a fertility restoration method. The amino acid sequence of TMS10 is represented by SEQ ID No.1. A conventional method is adopted to knock, change or inhibit TMS10 gene to reduce the expression level of TMS10 gene in common paddy rice species so as to obtain paddy rice male sterility line. The prepared paddy rice sterility line does not have abnormal phenotype during the vegetative growth phase; anther development is abnormal during the reproductive stage; the expressions of the phenotype are the same in the mutants of non-glutinous rice and japonica rice; the paddy rice is sterile at an average temperature of 28 DEG C, and paddy rice is fertile at an average temperature of 22 DEG C. The temperature sensitive characteristic, which is utilized during the process of two line hybrid seed production, is widely used in agricultural production.

This invention relates to a nuclear conditional male sterility system in Brassica napus. Embodiments of the invention provide for the (male sterile) prebasic female (MsMsrfrf), the (male fertile) maintainer line (msmsrfrf), the (male sterile) basic female line (Msmsrfrf), and hybrid lines. Further provided are methods for the production of those lines. Further embodiments of the invention relate to markers associated to the sterility, fertility and maintainer alleles and the use of those markers in providing a hybrid system.

The invention discloses application of a male sterility gene OsDPW3 and a rice fertility restoration method. The gene OsDPW3 is knocked out, changed or inhibited by using the conventional genetic engineering method or based on a CRISPR/Cas9 system, and the OsDPW3 gene expression level is decreased in wild type rice with japonica rice background, so that a rice male sterile line is further obtained. The invention also relates to the rice fertility restoration method. According to the method, the OsDPW3 gene is amplified by means of primers, and mutant plants are transformed by using a genetic transformation means, so that the mutant can be restored to a wild-type phenotype. The rice male sterility line prepared by the method has no abnormal phenotype in the rice vegetative growth period, but abnormality occurs in an anther development process of the reproductive growth period. If the rice male sterility line is applied to cross breeding, the work of castration of a female parent can beeliminated, so that the production efficiency is greatly improved, and the labor cost is lowered. The male sterility gene OsDPW3 has important application to agricultural production.

The invention provides a molecular marker for a paddy recessive genic male sterility gene cyp704b2 and application thereof, belonging to the technical field of plant biology. The molecular marker provided by the invention comprises two forward primers SEQ ID NO.1 and SEQ ID NO.2 and one reverse primer SEQ ID NO.3. The molecular marker can be utilized to complete the genetic typing of the paddy recessive genic male sterility gene cyp704b2 on the basis of conventional PCR and PAGE glue electrophoresis. The molecular marker has the advantages of simplicity and convenience in operation, high typing speed, accurate result and low cost, is capable of increasing the selection efficiency of the target trait and can meet the requirements of large-scale molecular marker-assisted selection breeding.

Metastin, compounds that promote the activity of metastin or its receptors and the like are excellent gonadal function improving agents, ovulation inducers or promoters, gonadotropic hormone secretion promoters, gonadotropic hormone secretion inhibitors, sex hormone secretion promoters, sex hormone secretion inhibitors, etc., and can be used as agents for preventing/treating sterility, hormone-sensitive cancers, endometriosis, etc. Metastin and its receptors are useful for screening for these pharmaceuticals.

The invention provides a rice CYP704B2 gene mutant and applications thereof, belonging to the technical field of genetic engineering. A nonglutinous rice variety 93-11 is subjected to induced mutation by cobalt 60 radiation, so as to cause deletion of two G basic groups behind 1267 basic groups of a rice CYP704B2 gene; the rice CYP704B2 gene mutant is named as cyp704B2-2, and a nucleotide sequence thereof is shown as SEQ ID No.1 and further verifies that the mutant causes rice recessive genic male sterility; the rice CYP704B2 gene mutant can be used for preparing transgenic rice with recessive genic male sterility, and plays an important role in genetic improvement and breeding of rice germplasm resources. The invention also provides a molecular marker identification method of the mutant and applications of the method in breeding and seed production.

The invention discloses a coated carrier and the application thereof in the method for detecting maturity of sperms and separating mature sperms noninvasively; the coated carrier is a carrier coated by hyaluronic acid, or hyalurate or hyaluronic acid derivatives; the detecting method is as follows: preparing specimens; loading the specimens to the coated carrier and covering with microscopic glass; reacting for 5-60 minutes at room temperature; observing by a microscope and counting; and calculating the combination ratio of hyaluronic acid of active sperms. The separating method is as follows: preparing specimens; loading the specimens to the coated carrier, reacting for 5-120 minutes at the temperature of 18-37 DEG C; and collecting the combined sperms. The coated carrier has the advantages of precise and reliable results, simple and fast operation, low cost and good safety performance, and can be used for estimating sperms quality, sperms maturity and analysis of the causes of sterility disease of mails, and is applicable to assisting reproduction or reproduction research center to safely collect mature and active sperms, so that the goal of improving embryo quality greatly is achieved.

The present invention relates generally to a system and methods for providing protective coverage of an operational surface and any items that may be on the surface. Certain embodiments of the system may be used to help to maintain the sterility of a surface and the items on the surface. Advantageously, such embodiments of the system may be used to maintain the sterility of surgical instruments and materials, and the table surface on which they are placed prior to the start of the surgical procedure. Other embodiments of the system may be used to at least help to prevent the contamination of or maintain the freshness of food and the containers and utensils used to hold, prepare, and serve the food.

The present invention discloses a breeding method for pod dehiscence-resistant cytoplasmic sterile line and pod dehiscence-resistant cytoplasmic maintainer line of brassica napus, and belongs to a cultivation method for a new crop variety. With the method provided by the present invention, the detection method for pod dehiscence resistance of the rape is improved; the pod dehiscence-resistant germplasm resource is screened; the screened pod dehiscence-resistant line H155 and the cytoplasmic sterile line 077A are subjected to hybridization, and continuous 8 backcrosses to breed the cytoplasmic sterile line H155A, wherein the sterility rate of the H155A is 100%. According to the present invention, the H155A has characteristics of micro pollen at the low temperature, complete sterility at the high temperature, good agronomic characteristics, cold resistance, viral disease resistance and sclerotium disease resistance. The erucic acid content and the glucosinolate content in the H155A is consistent with the erucic acid content and the glucosinolate content in the cytoplasmic maintainer line of the H155A. The pod dehiscence-resistant cytoplasmic sterile line and the pod dehiscence-resistant cytoplasmic maintainer line of the brassica napus establish the foundation for breeding the domestic pod dehiscence-resistant cytoplasmic sterile line hybrids.

The invention relates to isolation and cloning of paddy rice photoperiod sensitive genic male sterility gene pms1 and applications thereof. The pms1 gene has only one transcript PMS1T, which is a non-coding long-chain RNA. There are insertion/deletion variation as long as 65 bp and two mononucleotide mutations between different alleles of the gene, wherein the sequence of a dominant allele of the pms1 is represented as the SEQ ID No.4 and the sequence of a recessive allele is represented as the SEQ ID No.3. By means of the insertion/deletion variation and one mononucleotide mutation, two molecular markers of the paddy rice photoperiod sensitive genic male sterility gene pms1 are obtained. The dominant allele of the pms1 can reduce the setting rate of NIL (MH) under long-day condition, while excessive expression on total-length or shortcut PMS1T also can reduce the setting rate of NIL (MH) under long-day condition too, so that by inhibiting the expression of the PMS1T in a photoperiod sensitive genic male sterile line Nongken 58S, the fertility of the Nongken 58S can be recovered. The isolation and cloning of the gene has important utilization value for selective breeding and improvement of a novel paddy rice photoperiod sensitive genic male sterile line.