197 results about "Acid phosphatase" patented technology

The invention provides plant acid phosphatase coding sequences. Also provided are constructs comprising these sequences, plants transformed therewith and methods of use thereof. In certain aspects of the invention, transgenic plants are provided exhibiting improved phosphorous utilization.

The present invention relates to an isolated mutant acid phosphatase / phytase with improved enzymatic properties. The mutant acid phosphatase / phytase composition is particularly useful in animal feed compositions.

The invention discloses a combined detection kit for seminal plasma. The nine major indexes of pH value, leukocyte esterase, lecithin body, citric acid, zinc, acid phosphatase, elastase, fructose and neutral alpha-glucosidase in the seminal plasma can be rapidly, accurately, simply, conveniently and practically detected at one time in a combined way, functions of glands such as the prostate, the epididymis and the vesicula seminalis can be comprehensively evaluated, detailed related information is provided for the clinical diagnosis and treatment of caused related diseases such as prostatitis, reproductive system inflammation, sexual dysfunction and sterility infertility, a market gaps are filled, and the combined detection kit is easy to clinically popularize.

The invention discloses an acid phosphatase GmPAP1-like protein gene from the cell wall of soybeans and application thereof. The acid phosphatase GmPAP1-like protein gene has a nucleotide sequence asshown in SEQ ID No. 1 and an amino acid sequence as shown in SEQ ID No. 2. The acid phosphatase GmPAP1-like protein gene provided by the invention is capable of participating in activation and utilization of extracellular organic phosphorus, eventually improves the low-phosphorous resistance of plants and has strong acid phosphatase activity. Overexpression of the acid phosphatase protein gene inthe roots of crops can substantially improve the organic-phosphorus activation and utilization capabilities of transgenic roots; and thus, soybean roots can be improved in adaptive capacity to low-phosphorous stress of acid soil through gene transformation, and the acid phosphatase protein gene has good application prospects in construction of transgenic soybeans with resistance to low-phosphorousstress.

Alzheimer's disease is characterized by the presence of senile plaques formed from beta amyloid (Abeta), and neurofibrillary tangles (NTFs) formed from paired helical filaments consisting of hyperphosphorylated tau. A number of studies have shown that the NTFs correlate better with the duration and severity of Alzheimer's disease than senile plaques. However, a criticism of the primary etiological role of NTFs in Alzheimer's disease is the absence of variants of kinases or phosphatases associated with Alzheimer's disease. Acid phosphatase, a product of the ACP 1 gene, is a ubiquitous low molecular weight protein tyrosine phosphatase. A common allele, ACP 1*A, is associated with a lower activity of acid phosphatase. It is due to an Arg 105 Gln substitution of the ACP1 locus and detected as a Taq I polymorphism. We report a significant association of the low activity 2 allele with sporadic early onset Alzheimer's disease (EOAD). These findings support the possibility that other variants of kinase and genes may be associated with sporadic Alzheimer's disease.

The invention discloses a soil conditioner for tobacco field acidic soil, wherein the soil conditioner comprises, by weight, 40-80 parts of shells, 20-60 parts of potassium feldspar, 10-50 parts of limestone, and 15-40 parts of dolomite. According to the present invention, the soil conditioner can neutralize the active acid and the exchangeable acid in the soil, obviously increase the pH value ofthe soil, increase the soil plough layer exchangeable Ca<2+> and Mg<2+> concentrations, improve the soil microbial environment, increase the numbers of soil bacteria, actinomycetes and aerobic cellulose decomposition bacteria, improve the activities of urease, acid phosphatase, catalase and cellulase, passivate the heavy metals in the soil, reduce the heavy metals in tobacco, reduce the occurrenceof tobacco root diseases, improve the aroma quality, the aroma amount and the concentration of tobacco leaves, and reduce the irritation, the miscellaneous gas and the like of tobacco leaves.

A method for producing nucleoside-5'-phosphate esters inexpensively and in high yields by phosphorylating a nucleoside with a phospahte group donor using an acid phosphatase having an increased affinity for the nucleoside and / or an increased temperature stability at a pH of pH 3.0 to 5.5, to produce a nucleoside-5'-phosphate ester. Mutant acid phosphatases having increased affinity for nucleosides and / or an enhanced temperature stability are also provided.

The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a S. cerevisiae mating factor alpha 2 (Sc MFα2) or repressible acid phosphatase (Sc PHO5) secretion signal.

The invention discloses a kit for detecting the activity of acid phosphatase in seminal plasma and a detection method. The kit comprises substrate solution, a standard sample, stop solution and a color development reagent, wherein the substrate solution is disodium phenyl phosphate substrate solution; and the stop solution is alkaline solution. The detection method comprises the following steps of: first setting parameters of a detecting instrument; then adding the disodium phenyl phosphate substrate solution into an empty tube, adding the standard sample and the substrate solution into a standard tube, and adding the dilute seminal plasma and the substrate solution into a sample tube; next simultaneously performing incubation on the empty tube, the standard tube and the sample tube at the temperature of 37 DEG C for 15 minutes; and finally adding the same amount of alkaline solution and color development reagent into the incubated empty tube, the incubated standard tube and the incubated sample tube, performing uniform mixing, and performing color comparison by using the detecting instrument to obtain a result. The kit and the detection method of the invention can make the operation simple, convenient, time-saving and reagent-saving, and can make stable and reliable the result and be applied to laboratories for the department of andrology in various hospitals as synchronous comparison tests can be performed.