113 results about "Alkaline phosphatase" patented technology

Zeolite / clay / phosphate catalysts can be prepared by a process wherein a composition of zeolite-clay-phosphate is brought to a pH level of about 7.0 to about 14.0. The resulting slurry is then age reacted for about 0.5 to about 24 hours. Thereafter the slurry is dried to produce a zeolite / clay / phosphate catalyst particles that are particularly characterized by their high levels of zeolite stability.

The invention relates to a dental implant and a preparation method of a bioactive antibacterial surface of the dental implant. The dental implant is characterized in that medical titanium or a titanium alloy is taken a substrate; a nanotube morphology is constructed on the surface of the substrate through anodic oxidation; the surface of the substrate is uniformly coated with a polydopamine bionic membrane layer formed by auto-polymerization; the dental implant with the bioactive antibacterial surface provided with in-situ grafted silver nanoparticles is obtained by means of the adsorption and reduction capabilities of a polydopamine surface for silver ions. By adopting the dental implant, a uniform discrete nanotube oxide layer is formed in situ on the surface of the titanium substrate to be taken as a storage carrier of a large quantity of antibacterial silver particles, and the bioactive antibacterial surface which grows in situ is prepared through the steps of surface dopamine auto-polymerization and in-situ soaking and reduction and the like, so that the implant surface has more durable antibacterial property, the hydrophilic performance and bioactivity of the surface are improved, the alkaline phosphatase activity of osteoblast on the implant surface is increased, the differentiation of the osteoblast is facilitated, the infection risk of the implant after the implant is implanted is lowered, and the implanting success rate is increased.

The invention relates to an immunoassay detection kit, and provides an allergenic specific IgE antibody immunoassay detection kit and a preparation method thereof. The detection kit comprises magnetic microspheres coupled with allergenic protein, magnetic microspheres coupled with anti-human IgE antibody, a human IgE antibody standard substance, alkali phosphatase labeled anti-human IgE antibody and an alkali phosphatase chemical luminous substrate AMPPD. The preparation method for the detection kit comprises the following steps: coupling the allergenic protein to the magnetic microspheres, coupling the anti-human IgE antibody to the magnetic microspheres, preparing the human IgE antibody standard substance, preparing the alkali phosphatase labeled anti-human IgE antibody, preparing the alkali phosphatase chemical luminous substrate AMPPD and packing the combination. The detection kit has the advantages of high sensitivity, high accuracy, quick response, convenient usage, low dosage and low cost, can assist in precisely and quantitatively measuring sensitive allergene, and guides clinical anaphylactic reaction treatment.

The invention discloses an aptamer sensor based on nano particles and chemiluminiscence. The aptamer sensor takes Fe3O4@SiO2 magnetic nano particles and double-functional gold nano particles as solid-phase carriers; activated carboxylated Fe3O4@SiO2 magnetic nano particles and an amino modified aptamer are combined to form a magnetic nano particle-aptamer compound; then the magnetic nano particle-aptamer compound and the double-functional gold nano particles are subjected to hybridization to form a magnetic nano particle-aptamer-double-functional gold nano particle compound; finally, the magnetic nano particle-aptamer-double-functional gold nano particle compound is combined with streptavidin marked alkaline phosphatase to obtain the sensor. The invention further discloses a preparation method and an application of the sensor. The aptamer sensor has the magnetism and is convenient in rapid magnetic separation, also has the advantages of being rapid by adopting a chemiluminiscence method and efficient in detection, and has a very great application value in the detection aspect of biomedicines.

The invention relates to a magnetic particle chemiluminescence immunoassay method for human thyroglobulin antibodies (TGAb) and belongs to the technical field of immunoassay. TG antigens marked by fluorescein isothiocyanate (FITC) and human IgG antibodies marked by alkaline phosphatase are combined to form an antigen-antibody-second enzyme-labeled antibody sandwich immune complex with a sandwich-like structure. Then, magnetic particles connected with FITC antibodies are added, the antigen-antibody complex is connected to the magnetic particles through specific combination of the FITC antibodies and the FITC and directly deposited in an external magnetic field, and the complex formed by immune reaction can be separated from the other non-combined substances without centrifuging. A kit combines chemiluminescence with the magnetic particles to provide a reaction system close to a homogeneous phase. Compared with the prior art, the kit has the advantages of higher sensitivity, wide linear range, quickness and the like; the cost of a product is greatly reduced; and the kit has a broad application prospect on the aspects of clinical examination and the like.

The invention discloses a magnetic particle chemiluminescence quantitative assay kit for anti-ribosomal P protein antibody IgG. The kit includes: anti-ribosomal P protein antibody IgG calibrator; Ribosomal P protein antigen and bovine serum albumin-labeled Tris buffer; alkaline phosphatase-labeled goat anti-human polyclonal antibody and bovine serum albumin in Tris buffer; streptavidin-labeled magnetic particles and bovine serum albumin Serum albumin in Tris buffer; wash solution. Based on the traditional membrane strip immunoassay and enzyme-linked immunosorbent assay, the detection method of the kit increases the sensitivity and linear range by 3-5 orders of magnitude, and realizes quantitative detection in a real sense, with rapid response, reliable results, and It can be used in conjunction with a fully automatic chemiluminescence immunoassay analyzer to realize fully automatic use, and has irreplaceable important value for clinical diagnosis.

The magnetic separated AKP-labeled immunity analysis reagent comprises: the AKP label, the magnetic separation reagent as nano magnetic micro-ball covered by FITC, the FITC-antibody label, and the standard antigen or antibody and substrate. Wherein, the substrate can be PMP, AMPPD or APS-5. This invention is reliable and nun-polluted, and can detect fast and precisely.

The present invention provides methods of improving the osteogenic and / or chondrogenic activity of a bone matrix, e.g., a dermineralized bone matrix (DBM), by exposing the bone matrix to one or more treatments or conditions. In preferred embodiments the bone matrix is derived from human bone. The treatment or condition may alter the structure of the bone matrix and / or cleave one or more specific proteins. Cleavage may generate peptides or protein fragments that have osteoinductive, osteogenic, or chondrogenic activity. Preferred treatments include collagenase and various other proteases. The invention further provides improved bone and cartilage matrix compositions that have been prepared according to the inventive methods and methods of treatment using the compositions. The invention further provides methods of preparing, testing, and using the improved bone matrix compositions. On a assay comprises exposing relatively undifferentiated mesenchymal cells to a bone matrix composition and measuring expression of a marker characteristic of osteoblast or chondrocyte lineage(s). Increased expression of the marker relative to the level of the marker in cells that have been exposed to a control matrix (e.g., an inactivated or untreated matrix) indicates that the treatment or condition increased the osteogenic and / or chondrogenic activity of the bone matrix. Suitable cells include C2C12 cells. A suitable marker is alkaline phosphatase. The inventive methods increase the osteogenic and / or chondrogenic activity of human DBM when tested using this assay system.

The invention discloses a novel coronavirus SARS-CoV-2 S protein detection method. SARS-CoV-2 virus depends on spike protein on the surface to be combined with angiotensin converting enzyme 2 on the surface of a cell so as to enter the cell; according to the method, raw materials commonly used in chemiluminescence immunoassay and ACE2 are easy to purchase from the market, the biotin labeling and the alkaline phosphatase labeling are rapid, the reagent for SARS-CoV-2 S protein detection can be rapidly prepared, and the positive coincidence rate, the negative coincidence rate and the total coincidence rate are high compared with the SARS-CoV-2 fluorescence quantitative PCR result.

This invention provides a human hypothyroid peroxid enzyme antibody magnetic separation enzyme immune measurement method, whose steps are the following: to add sample to react with immune; to wash and to join two anti-enzyme indicate reagent and to wash again; to add monophosphatase fenolftaleina substrate solvent; to terminate color forming and to read absorbing value. The advantages in this invention are the following: it uses reformed human hypothyroid peroxid enzyme antigen fold immune magnetism micro ball as fixed phase and uses alkalescence phosphate enzyme as mark enzyme. Through the quantitative measurement of peroxid enzyme antigen of the hypothyroid patient and normal serum and other clinic symptom, it can judge whether the patient has got the self-immune hypothyroid illness.

The invention provides a fluorescence immunoassay method based on carbon quantum dots. The carbon quantum dot is prepared by using citric acid as a carbon source and thiourea or urea as a nitrogen source. The carbon quantum dot is applied to an enzyme-linked immunoassay method. A novel high-sensitivity fluorescence immunoassay method based on carbon quantum dots is established. An efficient fluorescence internal filtration effect is generated between carbon quantum dots and an enzyme-catalyzed chromogenic substrate, and an absorbance signal formed by reaction of horse radish peroxidase / alkaline phosphatase catalytic substrate in enzyme-linked immunoassay can be successfully and effectively converted into a fluorescence signal. The method has the advantages of simplicity in operation, low cost, high sensitivity, good stability and the like, and can be used for quantitative detection of the micromolecular hapten. In addition, the method has good expansibility and is expected to be expanded to other target detection systems.

The invention discloses a magnetic particle-based quantitative chemiluminescent assay kit for an anti-histone antibody IgG. The kit comprises an anti-histone antibody IgG calibrator, an anti-histone antibody IgG quality control product, a Tris buffer containing biotin-labeled histone antigen and bovine serum albumin, a Tris buffer containing an alkaline phosphatase-labeled goat anti-human polyclonal antibody and bovine serum albumin, a Tris buffer containing streptavidin-labeled magnetic particles and bovine serum albumin, and a rinsing solution. The detection method using the kit improves sensitivity and a linearity range by 3 to 5 orders of magnitudes on the basis of a traditional membrane strip immunization method and a traditional enzyme linked immunosorbent assay method, realizes real quantitative determination, is rapid in reaction and reliable in results, can be automatically used in cooperation with an automatic chemiluminescence immunity analyzer and is of irreplaceable important value to clinical diagnosis.

The invention discloses a traditional Chinese medicine feed additive and an application thereof. The traditional Chinese medicine feed additive disclosed by the invention is an animal feed additive, and the active ingredients of the animal feed additive comprise the following traditional Chinese medicine raw materials in parts by weight: 10-30 parts of lycium barbarum, 10-30 parts of wolfiporia cocos, 30-60 parts of fructus Hippophae, 30-60 parts of flos Lonicerae and 30-60 parts of polygonatum sibiricum red. The animal feed additive disclosed by the invention has the following advantages of reducing the content of cholesterol in eggs, increasing the contents of selenium, ferrum and zinc, increasing the contents of total protein in chicken serum, the content of globulin and the convent of alkaline phosphatase and increasing the content of complement C-4 in the chicken serum.

The invention relates to a nanometer magnetic particle chemiluminiscence kit, a preparation method and a detection method of a hepatitis B virus surface-antibody. The kit comprises a solution containing hepatitis B virus surface-antibody marked by fluorescein, a suspension liquid of the magnetic particle coated with a fluorescein antibody and a solution containing the hepatitis B virus surface-antibody marked by alkaline phosphatase. According to the invention, quantitative detection of the hepatitis B virus surface-antibody can carried out at lower cost, higher accuracy and higher precision.

Described herein are reprogramming techniques allowing for production of mammary-derived iPSCs (“m-iPSCs”). The m-iPSCs described herein exhibit all the hallmarks of stem cell identity including round cluster, bright colony morphology, clonal expansion, and pluripotent marker expression (alkaline phosphatase expression, Oct-4, nanog, etc.) Further refined techniques allow for generation of m-iPSCs under essentially defined conditions.

The invention discloses a high-affinity anti-carcinoembryonic antigen nanobody. The nanobody has three unique complementary determining regions CDR1, CDR2 and CDR3. The invention further provides an expression vector containing a nanobody variable region coding sequence and a host cell containing the expression vector, and further provides fusion protein of a nanobody variable region and alkalinephosphatase, the application of the nanobody to preparation of a carcinoembryonic antigen detection kit, a method for performing immunodetection on a carcinoembryonic antigen by applying the nanobody,and the corresponding detection kit. The anti-carcinoembryonic antigen (CEA) nanobody provided by the invention has a specific CEA recognizing and bonding ability, has the affinity which can reach 4.791E-10, and has unique antigen-determining cluster recognition sites; an excellent detection result can be obtained in CEA immunodetection, especially in a double-antibody sandwich method.

The invention discloses a procambarus clarkia feed additive as well as a preparation method and use thereof, belonging to the technical field of aquatic product feed additives. The procambarus clarkia feed additive comprises the following raw materials in parts by weight: 1 part of honeysuckle, 0.8-1.2 parts of radix astragali, 0.6-1.0 part of poria cocos and 0.7-1.1 parts of houttuynia cordata. The preparation method comprises the steps of weighing 1 part of honeysuckle, 0.8-1.2 parts of radix astragali, 0.6-1.0 part of poria cocos and 0.7-1.1 parts of houttuynia cordata according to parts by weight, respectively soaking, poaching and filtering the raw materials to obtain filtrates, respectively concentrating the filtrates to obtain honeysuckle concentrated solution, radix astragali concentrated solution, poria cocos concentrated solution and houttuynia cordata concentrated solution, mixing the concentrated solutions to obtain the procambarus clarkia feed additive. The procambarus clarkia feed additive has the advantage of remarkably improving the sum of blood corpuscles, the lysozyme, the superoxide dismutase, the acid phosphatase and the alkaline phosphatase of the procambarus clarkia to ensure the healthy growth and reduce the death rate by using the honeysuckle, the radix astragali, the poria cocos and the houttuynia cordata to form the additive.

The invention discloses multiple PCR detection primers of escherichia coli, pseudomonas aeruginosa and staphylococcus aureus, a kit and a detection method. Specific primers are designed by means of combined utilization of escherichia coli alkaline phosphatase gene, pseudomonas aeruginosa outer membrance proteins gene and staphylococcus aureus heat-resistant nuclease gene sequences, multiple PCR detection is conducted on a sample by means of the specific primers according to the method, and specific detection of the escherichia coli, the pseudomonas aeruginosa and the staphylococcus aureus in the sample can be achieved at a time; the detection time is short, operation is easy and convenient, the detection efficiency can be effectively improved, the requirement of rapid detection is met, the sensitivity is high, the specificity is high, and the multiple PCR detection primers of the escherichia coli, the pseudomonas aeruginosa and the staphylococcus aureus, the kit and the detection method can be applied to microbiological detection work of cosmetics.

The invention discloses a fusion gene fragment rolB-FGFs, which consists of a rolB gene and an FGFs gene that is designed according to the preference of a plant codon, wherein a PIM gene without the antibiotic marker and a human secreted alkaline phosphatase signal peptide (SEAP) gene are introduced; a plant secreted binary expression vector without the antibiotic marker is constructed; the expression vector is transformed into agrobacterium rhizogene; by taking the plant as the host and adopting a hairy root system to express the FGFs, the active FGFs can be purified from a hairy root and culture solution respectively, and the FGFs can be prepared industrially, thereby improving the inductivity, the expressed protein amount, the yield and the stability of the hairy root; and no antibiotic marker exists in the method, thereby protecting the environment. The ginseng hairy root containing the FGFs prepared by taking the ginseng as the host has the double pharmacological effects of the ginseng and the FGFs, thereby being a better health-care product or medicine; in addition, the ginseng hairy root is used for the development and the application of the functional food and medicine and has wide prospect.

The technology belongs to the technical field of aquiculture disease prevention, in particular to the preparation method of the immune reinforcing agent for cultivating crustacean, and the applicationmethod thereof. The aquiculture composite immune reinforcing agent comprises 2-4 parts of aquiculture extractive or baicalin and 2.5-5 parts of levamisole. The composite immune reinforcing agent caneffectively enhance non-specific immunity for cultivating blue crab, improve the activity of the blood serum phenol oxidase, the superoxide dismutase and the alkalinephosphatase of the blue crab, andimproves the disease resistance of the blue crab to common pathogens such as white spot syndrome virus, vibrio, hematodinium, etc. The composite immune reinforcing agent can be applied to the cultivation of the blue crab by being injected or added into blue crab fresh fish bait and granulated feed for preventing and controlling the disease at the disease peak and reducing the disease rate and themortality rate of the blue crab. The immune reinforcing agent is added into the feed for preventing and controlling the disease of the penaeus vannamei boone and exopalaemon carinicauda.

The invention provides a recombinant microorganism for producing beta-nicotinamide mononucleotide (NMN) and a method for producing NMN by using the recombinant microorganism, the strain of the recombinant microorganism contains one or more or all of the following characteristics: (1) adding nicotinamide into a fermentation culture medium, and transforming by the recombinant microorganism to generate NMN; and (2) the nicotinamide phosphoribosyltransferase is overexpressed. And (3) deletion or inactivation or enzyme activity reduction of genes for encoding alkaline phosphatase and nucleotidase on the recombinant microorganism genome.

The invention relates to a reagent kit for detecting tuberculosis antibodies, and a preparation method thereof. The kit is composed of a chain avidin coated solid-phase vector, negative and positive controls, biotinylation tuberculosis antigens, alkaline phosphatase marker tuberculosis antigens, concentration washing solutions and chemiluminescent substrate solutions. The reagent kit adopts the double antigen sandwich reaction principle and the biotin-avidin system coated solid-phase vectors, the method not only has uniform and firm coating and greatly improves the antigen purity, but also can increase the antigen absorption, lower the cost and have the characteristics of high sensitivity, strong specificity and the like, can accurately detect the tubercubosis antibodies in various tuberculosis serums, and provide a reliable basis for clinical diagnosis and tuberculosis screening.

The invention discloses a detecting card for bone alkaline phosphatase, hemoglobin and glutamic-pyruvic transaminase. The detecting card comprises a PVC (polyvinyl chloride) bottom plate, a sample pad, a fluorescent pad, a nitrocellulose membrane, a detecting line, a quality control line, water absorption filter paper, sample feeding ports, sample adding tubes, a support, a stop piece, a sample adding zone, a reaction zone, a sample flowing pad, a bulge, a reaction film and an added sample filtering zone, wherein the sample pad, the nitrocellulose membrane, the fluorescent pad, the water absorption filter paper, the added sample filtering zone, the sample flowing pad, the bulge, the sample adding zone and the reaction zone are arranged on the PVC bottom plate; one end of the sample pad is overlapped on the fluorescent pad, the other end of the fluorescent pad is overlapped on the nitrocellulose membrane, the water absorption filter paper is overlapped at the other end of the nitrocellulose membrane, the detecting line and the quality control line are formed on the nitrocellulose membrane, the added sample filtering zone and the sample flowing pad are contacted, and the sample adding tubes are arranged above the sample pad, the added sample filtering zone and the sample adding zone. The detecting card has the advantages of simplicity, convenience and rapidness in operation and the like.

The multi-constituent immunity analysis and detection system comprises: a solution transmission system with a multi-channel peristaltic pump (6) and a connection pipe (7) and a multi-position selection valve (8), an immunity reactor, a chemiluminescence detector, and a computer, wherein the analysis method comprises: fixing the packed antibody in the reactor, adding sample and enzyme-mark antibody; marking with HRP and alkaline phosphates to form two types of sandwich composites; clearing, crossing in turns the HRP chemiluminescence substrate area, cleaning buffer liquid area and the alkaline phosphates chemiluminescence substrate area. This invention has fast speed and high sensitivity for wide application.

The invention discloses a magnetic particle chemiluminescence quantitative determination reagent kit of anti-centromere antibody IgG. The reagent kit comprises an anti-centromere antibody IgG calibration material, an anti-centromere antibody IgG quality control product, a Tris buffering solution containing biotin-labeled centromere antigens and bovine serum albumin, a Tris buffering solution containing alkaline phosphatase-labeled goat-anti-human polyclonal antibodies and bovine serum albumin, a Tris buffering solution containing streptavidin-labeled magnetic particles and bovine serum albumin, and a cleaning solution. According to a detection method of the reagent kit, on the basis of a traditional membrane strip immunization method and enzyme linked immunosorbent assay, sensitivity and the linear range are improved by 3-5 magnitude orders, quantitative determination in real sense is achieved, responding is fast, the result is reliable, and the magnetic particle chemiluminescence quantitative determination reagent kit can be full automatically used in cooperation with a full-automatic chemiluminescence immunity analyzer and has irreplaceable important value on clinical diagnosis.

The invention relates to the technical field of carbon quantum dots, in particular to preparation of cellulose-based carbon quantum dots and application of the cellulose-based carbon quantum dots in the field of organophosphorus pesticide (OPs) detection. The nitrogen-doped blue fluorescent carbon dots are synthesized by taking microcrystalline cellulose and aspartic acid as raw materials through adoption of a one-step hydrothermal method. Characterization is carried out by means of a transmission electron microscope, an ultraviolet spectrum, a fluorescence spectrum and the like. On the basis of an internal filtering effect (PER), by virtue of an enzymatic reaction of decomposing 4-nitrophenyl sodium phosphate (NPP) through alkaline phosphatase (ALP), carbon dot fluorescence is quenched by taking NPP as an absorber, and the fluorescence is recovered along with continuous generation of a product 4-nitrophenol (NTP). The invention also provides a novel organophosphorus pesticide detection method, when OPs exists in a detection solution, the decomposition progress of NPP can be influenced, and the fluorescence recovery level has different degrees of differences. Through the change of the fluorescence intensity, whether the organophosphorus pesticide exists in the daily water or agricultural products or not is visually judged, so that the purposes of protecting the environment and maintaining the body health are achieved.

The invention relates to an antibody chip for screening and diagnosing compositae plant virus diseases, and a kit and a method of the antibody chip. The antibody chip for screening and diagnosing compositae plant virus diseases is mainly characterized by comprising a base membrane and specific capture antibodies (primary antibodies) which are respectively pointed on the base membrane and used for capturing seven types of viruses with harm to the compositae plant, wherein the antibodies (primary antibodies) for capturing seven types of viruses have resistance to bean yellow mosaic virus, resistance to arabis mosaic virus, resistance to curtobacterium flaccumfaciens virus, resistance to cucumber mosaic virus, resistance to tobacco rattle virus, resistance to tobacco ringspot virus and resistance to tomato spotted wilt virus; three positive quality control points P, IgG pointed to be alkaline phosphatase mark, three negative quality control points N and a carbonate buffer solution pointed to reach a pH being 9.2-9.8 are also encapsulated on the base membrane. The antibody chip for screening and diagnosing compositae plant virus diseases has the advantages that the antibody chip for detecting a plurality of viruses of the compositae plant is successfully built, so that comprehensive detection is carried out on a plurality of pathogens at a high flux.

The invention discloses a medical implant and a preparation method thereof. The method comprises the following steps: firstly, constructing a special memory deformation high polymer material with pores, then fixing the special memory deformation high polymer material on the surface of an implant body, and carrying out volume expansion on the memory deformation high polymer material under illumination or temperature stimulation to obtain the degradable expansion implant. Compared with an existing medical implant, the implant is jointly composed of an outer-layer deformable material and an innerimplant body, and after the implant is implanted into sclerotin, the outer-layer high polymer material of the implant expands in a manual regulation and control mode, so that the high initial stability of the implant is achieved. Due to the degradability and the growth factor carrying capacity of the material, the synthesis of bone cell alkaline phosphatase and the deposition of calcium-containing minerals can be effectively promoted, and osteogenesis is induced, so that the rapid and effective osseointegration effect of the implant is finally realized.

The invention discloses a preparation method of a photoelectrochemical furazolidone sensor, and belongs to the technical fields of novel nano functional materials and biosensors. According to the preparation method, a novel dual metal co-doped two-dimensional photosensitizer is prepared at first, namely, iron and manganese co-doped titanium dioxide nano blocks and molybdenum disulfide carry out in-situ synthesis to generate a two-dimensional nano composite material (FeMn-TiO2 / MoS2), which has a good biocompatibility and large specific area, then a furazolidone antibody is loaded on the two-dimensional nano composite material, alkaline phosphatase is fixed on the two-dimensional nano composite material; when the photosensitizer is used for detection, alkaline phosphatase can catalyze L-ascorbic acid-2-phosphate trisodium salt (AAP) to generate L-ascorbic acid (AA) in-situ; thus electron donors are provided for photoelectric detection; due to the influence of specific and quantitative combination between antibody and antigen on the electron transmission performance, the photoelectric current strength is correspondingly reduced, and finally a photoelectric sensor for detecting furazolidone through a label-free photoelectric method is manufactured.