210 results about "White spot syndrome" patented technology

The invention discloses a Chinese herbal medicine immunopotentiator for resisting white spot syndrome virus (WSSV) of procambarus clarkia, as well as a preparation method and an application thereof. The preparation method comprises the following steps: milling rheum officinale, epimedium, astragalus membranaceus and isatis root and fully mixing the ingredients in proportion to prepare the powdery immunopotentiator. The Chinese herbal medicine immunopotentiator disclosed by the invention is stable and wide in raw material resources, simple to prepare, and low in cost, is based on the immunomechanism of shellfish to provide Chinese herbal medicines for the shellfish, and can be used for improving the immunity and oxidation resistance of a body during application, improving the capacity of the procambarus clarkia for resisting WSSV infection, and promoting the growth of the procambarus clarkia.

An expression cassette, wherein said expression cassette comprises: (a) an ie 1 promoter from white spot syndrome virus or a variant thereof; (b) a nucleic acid sequence encoding an N-terminal gp64 signal peptide or variant thereof, a nucleic acid sequence encoding an antigenic peptide, a nucleic acid encoding a transmembrane region, and a nucleic acid encoding a gp64 cytoplasmic region or variant thereof; in which the promoter is operably linked to the nucleic acid sequence.

A compound Chinese herbal medicament preparation for preventing and treating white spot syndrome is prepared by following raw materials by weight: 20-35 parts of Corydalis bungeana Turcz., 25-40 partsof radix isatidis, 15-40 parts of radix astragali, 20-40 parts of dyers woad leaf, 25-35 parts of wrinkled giant hyssop, 10-30 parts of Japanese eupatorium, 15-25 parts of roughhaired holly root and5-15 parts of Gynostemma pentaphylla. The compound Chinese herbal medicament preparation can effectively prevent and treat the white spot syndrome. The invention also discloses a forage comprising thecompound Chinese herbal medicament preparation and a method for feeding the same. The forage comprising the compound Chinese herbal medicament has simple processing technology and convenient feeding.The method for feeding the forage comprising the compound Chinese herbal medicament is scientific and reasonable and has a good antiviral effect.

The present invention is prawn white spot syndrome virus gene diagnosing kit and its usage. The kit and its usage is designed based on two pairs of primers for the conservation sequence of prawn white spot syndrome virus gene. By means of PCR technology, the present invention detects qualitatively the specific DNA nucleic acid segment of prawn white spot syndrome virus fast, simply, specifically and sensitively. The present invention may be used in the tracing detection of different stages of prawn cultivation and environment monitoring.

The present invention relates to a composition useful as prophylactic and/or therapeutic agent for the management of viral and bacterial diseases in aquatic animals, said composition containing effective amount of extract obtained from the plants Lantena camera, Aegle marmelos, Ocimum sanctum, Mimosa pudica, Cynodon dactylon, Curcuma longa, and Allium sativum, optionally in combination with a pharmaceutically acceptable carrier, diluents or excipients.

The invention relates to a method for preparing a recombining spore of surface display foreign protein, in particular to a recombining spore for surface display prawn white spot syndrome virus Vp28 protein of a bacillus subtilis spore. In the method, a bacillus subtilis spore capsid protein gene is used as a molecule vector and is recombined with an antigen protein gene on the surface of the prawn white spot syndrome virus to construct an integral recombining plasmid in fusion expression; the bacillus subtilis is converted to a bacillus subtilis recombining strain; and the recombining spore of the spore surface display prawn white spot syndrome virus protein produced by the recombining strain is induced.

The invention discloses a genetic engineering protein TAT-VP28-GH capable of resisting white spot syndrome virus (WSSV) as well as preparation and application thereof. The sequences of the genetic engineering protein are a nucleotide sequence shown in SEQIDNO:1 and an amino acid sequence shown in SEQIDNO:2; and the strain of the genetic engineering protein is a recombinant genetic engineering strain EscherichiacoliBL21pEt-32A-TAT-VP28-GH. The preparation method comprises the following steps: extracting WSSV from prawn, and obtaining VP28 gene by a polymerase chain reaction (PCR); amplifying aTAT-VP28 sequence through the PCR; extracting total mRNA (messenger RNA) from hypophysis cerebri of carp, and obtaining total cDNA through RT-PCR (reverse transcription-PCR); obtaining a mature peptide gene of carp growth hormone through PCR, inserting acid hydrolysis site aspartate and proline (Asp-Pro-Asp) in the upstream of the mature peptide gene; then constructing a recombinant plasmid pEt-32A-TAT-VP28-GH; and transforming the recombinant plasmid pEt-32A-TAT-VP28-GH to Escherichia coil BL21, and efficiently expressing fusion protein TAT-VP28-GH in a dissoluble form under the induction of isopropyl beta-D-1-thiogalatopyranoside (IPTG). The fusion protein can effectively prevent the prawn from suffering WSSV and promoting growth development of the prawn.

The invention discloses a kit for detecting white spot syndrome virus (WSSV) of prawn. The kit comprises DNA extractant of a sample to be detected, LAMP pre-reaction liquid, Bst DNA polymerase, LAMP reaction stabilizing solution, indicator, positive control DNA and negative control DNA. The method also discloses a method for detecting WSSV by utilizing the kit. The kit of the invention has strong specificity and can be applied to scientific research and WSSV detection of cultivation site. The method of the invention is low in cost, and is rapid and sensitive, and the detection result can be judged directly by naked eyes.

The invention relates to a method for breeding the FanNaBing sterilize which can resist hickie complex symptom virus. Wherein, it comprises: (1) selecting parent shrimp while the female-male ratio is 1:2; (2) counteracting toxic substances that ejecting hickie complex symptom virus solution into the first section of muscle at the back of parent; (3) eliminating the ill and dead parents, to select the FanNaBing sterilize which can resist hickie complex symptom virus; (4) breeding parent shrimps.

The invention discloses an envelope protein VP28 idiotype monoclonal antibody against shrimp white spot syndrome virus (WSSV) and a preparation method thereof. The antibody is secreted by a hybridoma cells with the collection number of CCTCC-CT200938, is prepared by taking anti-WSSV-VP28 monoclonal antibody (Ab1) as antigen, can bind with anti-WSSV-VP28 antibody of hare, and has the capability of competing with WSSV to bind with the anti-WSSV-VP28 antibody of the hare. The anti-WSSV-VP28 idiotype monoclonal antibody (Ab3) prepared by taking the antibody as antigen can bind with the WSSV, the binding site of the anti-WSSV-VP28 idiotype monoclonal antibody (Ab3) and the WSSV is located on an envelope, and the Ab3 can neutralize WSSV infection and has Ab1 properties. In the invention, the idiotype antibody is applied in the research of WSSV for the first time; a screening system is established, which uses an indirect enzyme-linked immunosorbent assay (ELISA) method and a competitive enzyme-linked immunosorbent assay (ELISA) method for detection; the fact that Ab3 has properties of Ab1 is proved by adopting an indirect immnnofluotesent method (IIF), a gold labeling immunoelectron microscopic method and crayfish in vivo neutralization tests, thus proving that the monoclonal antibody in the invention has the property to simulate original antigen WSSV-VP28.

The invention relates to a shrimp white spot syndrome virus disease detection reagent kit and a detection method thereof. The reagent kit consists of a set of primers, a DNA extracting buffer solution, DNA polymerase, a loop-mediated isothermal amplification reaction liquid, positive contrast, negative contract, betaine and dNTP. The reagent kit can detect whether a shrimp to be detected is infected by a shrimp white spot syndrome virus. The reagent kit utilizes the strand displacement characteristic of Bst DNA enzymes, designs four primers to identify six regions of a target sequence, generates a large number of stem-loop sample structures under the isothermal condition, and does not require any special equipment under the condition of qualitative detection, thereby having the characteristics of specificity, high sensitivity and short detection time compared with common PCR.

The invention relates to a detection kit for isothermal amplification of nucleic acid for the white spot syndrome virus and a detecting method thereof. The kit is designed by using a set of LAMP primers as the main body designed according to the gene conserved sequence of the white spot syndrome virus. The kit is provided with eleven agents needed by WSSV detection including SEMP lapping liquid, nucleic acid extract, UNG enzyme, TE buffer solution, enzymolysis buffer solution, LAMP reaction solution and on the like, thus realizing a programmed and standardized detection; therefore, the invention has the advantages of the highest sensitivity, simplicity, rapidity, safety, good specificity and low cost, and the amount of the copies detected with virus can be as low as 26. Only one water-bath is needed to correctly detect the white spot syndrome virus in prawns and aquaculture water within 2 hours by the kit and the detecting method. Moreover, the problem that the detection is subject to interference in the LAMP technology is also solved. The detecting method of the invention is expected to substitute the previous related detecting methods of the white spot syndrome virus, such as the electron microscope method, the TE staining method, the biopsy method, the antibody detecting method, the nucleic acid probe hybridization method and the PCR detecting method.

The invention discloses an indoor cultivation method of litopenaeus vannamei parent, and relates to the breeding of seawater shrimps. The method disclosed by the invention selects the healthy shrimp seed without carrying white spot syndrome virus, and is characterized in that the parent cultivation is performed in a cement nursery pond in a seed cultivation room; the water change amount of the cement nursery pond is adjusted according to the body length of the shrimp seeds; the shrimp seeds are fed with compound feed before the body length reaches 10cm; the shrimp seeds are fed with the mixture of compound feed and fresh shredded squid when the body length reaches 10cm; when the body length reaches 12cm, the shrimp seeds are fed with fresh shredded squid; after the shrimp seeds are longer than 14cm, the spermatophore of the male shrimp occurs, the shrimp seeds are fed with fresh fait with gonad of clamworm and oyster meat; before the shrimp seeds reach the parent specification, the cultivation salinity is kept at 22; when the shrimp seeds reach the parent specification and the spermatophore of a few male shrimps is mature, the salinity is adjusted to 30; and in the next day after a great amount of water in the nursery pond is changed, 10ml/m<3> of composite bacterial liquid consisting of bacillus subtilis, lactic acid bacteria and saccharomycetes is added once per day.

The present invention relates to a novel composition useful for inhibiting White Spot Syndrome Virus (WSSV) infection of crustacean animals, particularly those of the genera Penaeus sp. More specifically, the novel composition comprises a polypeptide whose amino acid sequence corresponds to at least a portion of Vp28, a surface protein of WSSV, or an antibody that specifically binds the polypeptide. The polynucleotide sequences encoding the Vp28 polypeptides of the present invention are also disclosed. Further disclosed are methods for using the novel compositions to inhibit WSSV infection in crustacean animals.

The invention discloses an immunity detection chip of prawn white spot syndrome virus (WSSV), comprising a chip carrier and an agarose gel layer which is paved on the chip carrier, wherein, the agarose gel layer is fixed with a plurality of antibody microarrays of 4*4, and chip-dedicated fences or Super PAP Pen scribings are used for separating the microarrays from each other. The invention comprises the following steps: adopting the sandwich method to detect the antigen; fixing the pathogenic polyclonal antibody (PcAb) on the chip slice base; taking the target organ tissue of the individual to be detected to prepare to-be-detected sample liquid; incubating directly the to-be-detected sample liquid with the chip which is fixed with PcAb to capture the antigen and lead the antigen to combine on the chip; adding a specific monoclonal antibody probe marked by fluorescence; and reading results through a CCD chip scanner. The invention has the advantage of detecting white spot syndrome virus (WSSV) in multiple samples simultaneously, and is applicable to the rapid and accurate detection of white spot syndrome virus (WSSV) of prawns/crabs in breeding production and the quarantine inspection of WSSV in import and export prawns/crabs.

The technology belongs to the technical field of aquiculture disease prevention, in particular to the preparation method of the immune reinforcing agent for cultivating crustacean, and the applicationmethod thereof. The aquiculture composite immune reinforcing agent comprises 2-4 parts of aquiculture extractive or baicalin and 2.5-5 parts of levamisole. The composite immune reinforcing agent caneffectively enhance non-specific immunity for cultivating blue crab, improve the activity of the blood serum phenol oxidase, the superoxide dismutase and the alkalinephosphatase of the blue crab, andimproves the disease resistance of the blue crab to common pathogens such as white spot syndrome virus, vibrio, hematodinium, etc. The composite immune reinforcing agent can be applied to the cultivation of the blue crab by being injected or added into blue crab fresh fish bait and granulated feed for preventing and controlling the disease at the disease peak and reducing the disease rate and themortality rate of the blue crab. The immune reinforcing agent is added into the feed for preventing and controlling the disease of the penaeus vannamei boone and exopalaemon carinicauda.