The invention relates to a field fast high-sensitive detecting kit of monodon baculovirus and a detecting method. The detecting kit comprises a sampling pipe, a rinsing pipe internally filled with distilled water, a nucleic acid denaturation pipe internally filled with TE cushion fluid, an amplified detecting pipe internally filled with amplified reaction liquid and nucleic acid dye, a negative tube, a positive tube, an FTA membrane, a fast drying liquid thereof, etc. Compared with the common PCR detecting method, the detecting method has higher specificity, sensitivity and convenience, has extreme low cost, nontoxicity, safety, convenient use, more exact detecting, fastness and extreme high sensitivity, and can replace the relevant existing detecting method such as a pathological section method, an electronic speculum observation method, an antibody detecting method and a PCR detecting method. The detecting kit and the detecting method not only can be used for an indoor laboratory, but also can be used for a wild production field, have significant meaning for enhancing epidemiological surveillance and disease control as well as prevention to the monodon baculovirus, and have extreme high population application value.