1619 results about "Penicillin" patented technology

The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

Polymers are disclosed that are chemically modified to retard bacterial growth. Such modified polymers (e.g. ePTFE and polypropylene) are produced by first creating acid groups on the polymer surface through reactions with an anhydride. The acid groups are then linked to polyethylene glycol (PEG) through esterification or other reactions such as amidation. Preferably, at least two different molecular weight PEG species are employed. The antimicrobial surface is completed by linking antibiotics (e.g. β-lactam antibiotics) to the PEG extensions. One preferred embodiment of such a modified polymer is produced using ePTFE, maleic anhydride (MA), and penicillin (PEN) to yield PEN-PEG-MA-ePTFE, which inhibits gram-positive bacteria. The PEG spacer is critical for PEN function in this context, since PEN-ePTFE is ineffective against bacterial growth. Another preferred embodiment incorporates ampicillin (AMP) and a heterobifunctional PEG, HOOC—(CH2—CH2—O)n—NH2, to yield AMP-PEG-MA-ePTFE. This latter example inhibits both gram-negative and gram-positive bacteria.

The invention discloses a method for preparing activated carbon from penicillin or terramycin strain residues. The method comprises the steps of: crushing strain residues, sieving by a 20-meshed sieve, uniformly mixing the strain residues with a solution prepared according to different activation ratios, soaking at a constant temperature for 24 hours, drying in an oven at the temperature of 105-110 DEG C, placing in a temperature resistant sealed container to prevent oxidization and incineration, placing the sealed container in an activating and carbonizing furnace, heating to an activation temperature, activating at the constant temperature for set time, washing the activated strain residues with hydrochloric acid, then washing with water to be neutral, drying at the temperature of 105-110 DEG C for 2-3 hours, and cooling to room temperature to obtain the strain residue activated carbon. According to the invention, prepared activated carbon is high in specific area, rich in micropores and mesopores and excellent in adsorbing performance; and waste terramycin strain residues from a pharmaceutical factory are utilized to prepare activated carbon, thus the resource, reduction and harmless treatment of the waste strain residues are effectively realized, and the social benefit is significant.

The invention discloses a method of preparing a bombyx mori silk fibroin specific antibody by utilizing a characteristic polypeptide. The method comprises the following steps: synthesizing a "CGAGAGSGAGAGS" polypeptide sequence by utilizing an Fmoc method, coupling the polypeptide with keyhole limpet hemocyanin (KLH) through the cysteine on the N terminus of the polypeptide so as to obtain a complete antigen; diluting the complete antigen with normal saline, mixing the diluted complete antigen with a complete Freund's adjuvant, adding streptomycin and penicillin to carry out an emulsion treatment so as to obtain a primary immunized antigen emulsion, subjecting a rabbit to a primary immunization by using the primary immunized antigen emulsion, then subjecting the rabbit to a strengthened immunization, wherein the strengthened immunization uses a strengthened immunized antigen emulsion, which is prepared by the following steps: mixing the diluted complete antigen with an incomplete Freund's adjuvant, and then adding streptomycin and penicillin to carry out an emulsion treatment so as to obtain the target product; collecting the blood of the immunized rabbit, when the antibody titer in the rabbit blood sample reaches 1 / 10000; making the blood blocks fully contract to completely separate out the antiserum, then collecting the antiserum, and subjecting the antiserum to a centrifugation treatment so as to obtain a supernate.

The invention relates to a separation method and a culture method for umbilical cord mesenchymal stem cells. The separation method comprises the following steps: thoroughly cleaning umbilical cord tissue of a healthy newborn by using a PBS (phosphate buffer solution) containing streptomycin and penicillin, and removing blood; shearing the umbilical cord into small sections uniform in length, and mechanically separating, bluntly stripping Wharton' s jelly, and removing umbilical arteries and umbilical veins; uniformly shearing the Wharton' s jelly; re-suspending the sheared Wharton' s jelly through an MSCs (mesenchymal stem cells) culture medium, inoculating to a culture dish with laid gelatin, and putting in a CO2 culture box for cultivation; conducting centrifugal separation to obtain tissue blocks and a cell resuspension solution. The culture method comprises the following steps: enwrapping the culture dish, discarding the gelatin, and washing with the PBS; inoculating the separated out tissue blocks and the cell resuspension into the culture dish; performing digestive subculture after cell fusion growth rate reaches 80-90%.

The invention relates to a medical sterile automatic-dispensing workbench for clinical transfusion. A main working area of the medical sterile automatic-dispensing workbench comprises a penicillin bottle opening system, an ampoule bottle opening system and an automatic dispensing system, wherein a sliding platform (7) is provided with at least four groups of penicillin bottle holding holes and at least four groups of ampoule bottle holding holes respectively, the lower parts of holding holes are connected with opened cylinders (8) of various specifications with openings, a homodromous rotatable shaft (9) is arranged beside each cylinder and is fixedly provided with a group of torsional springs (10), steel wires at the other ends of the torsional springs (10) are placed in the openings of the cylinders (8), each rotatable shaft (9) is fixedly provided with a connecting rod (11) and is further connected onto a cross rod (12), and one of the rotatable shafts (9) is provided connected with a motor in a connection manner to form a linkage bottle abandoning device; and a shelter eave (4) is arranged above the sliding platform (7), and an purified air outlet (6) is connected with an air purification system. The medical sterile automatic-dispensing workbench disclosed by the invention is used for air sterilization and local air purification in a dispensing room to meet a 100-grade purification requirement, and can be used for automatically opening penicillin bottles and ampoule bottles, sterilizing main surfaces of the bottles and performing medicine dispensing automatically.

The invention provides a mycoplasma hyopneumoniae culture medium and a preparation method thereof, belonging to the technical field of veterinary biology. The mycoplasma hyopneumoniae liquid culture medium comprises the components as follows: brain heart infusion, lactalbumin hydrolysate, PPLO (pleuropneumonia-like organism) broth, yeast extract powder, proteose peptone, sodium thiosulfate, Hank's liquid, sodium pyruvate, 0.1% phenol red solution, penicillin and deionized water. The preparation method comprises the following steps of: adding health horse serum before using, and adding agar into the liquid culture medium to obtain a solid culture medium of mycoplasma hyopneumoniae. The viable bacteria titer of the mycoplasma hyopneumoniae culture medium can reach 1*109CCU / ml-1*1010CCU / ml; the viable bacteria titer and the separation sensibility are far higher than those of the existing culture medium, and the mycoplasma hyopneumoniae is fast in growth speed and high in the separation sensibility; and the preparation method of the culture medium is simple in technology, strong in operability, and suitable for industrial large-scale production.

The invention provides an inducing method and inducing culture medium for differentiation of bone marrow mesenchymal stem cells into osteoblasts in vitro. The inducing culture medium is composed of 1*10<-8> mol / L of dexamethasone, 50 mu mol / L of ascorbic acid and 10 mmol / L of sodium beta-glycerophosphate; and solvent is a supernatant of a sclerite complete culture medium and comprises 10% of fetal calf serum, 100 U / mL of penicillin, 100 mg / L of streptomycin, a mixture of DMEM culture fluid and F12 culture fluid and multiple growth factors secreted by bone cells in the sclerite culture process. According to the invention, bone marrow mesenchymal stem cells of a mouse are purified by replacing the cell culture fluid through an adherent cell passage method, the obtained cells of the first generation are induced, and the supernatant of the sclerite complete culture medium cultured for 72-96 hours is used as the solvent of osteoblast differentiation inducer, thereby obviously improving the in vitro osteogenic differentiation efficiency of bone marrow mesenchymal stem cells.

The invention relates to a herbal medicine preparation method for the children bronchopneumonia treatment, belonging to the technical field of Chinese herbal medicine preparation methods; which adopts the technical proposal that: herbs like ginkgo seed, ephedra, apricot kernel, perilla, pinellia ternate, Swallowwort Rhizome (SRh), aster, Tussilago farfara, cortex mori, Chinese wolfberry root-bark, scutelarria, blackberry lily, dyers woad leaf, isatis root, bupleurum, dandelion, honeysuckle, houttuynia cordata, sargentgloryvine stem, sophora tonkinensis, APN (Andrographis Paniculta), Franchet Groundcherry Fruit, balloonflower and liquorice are immersed in 1000ml water for half an hour, and then decocted on mild fire; after decoctation, the herbal liquor is filtered to get 300ml herbal medicine for the treatment of children bronchopneumonia. In prior art, penicillin, streptomycin, and gentamicin are used for the treatment of children bronchopneumonia; the application of penicillin can cause allergic reaction, as a result, the life of the patient may be in danger if rescue is not timely. The preparation method has the advantages of simple manufacturing technology, small side effect and durable and stable therapeutic effect.

The invention provides a device for controlling a penicillin bottle to automatically enter and exit from a freeze dryer. The device is arranged at the inlet / outlet of the freeze dryer and comprises a frame, a push / pull mechanism, a bottle feeding track, a bottle storage platform and a bottle storage frame, wherein the bottle storage frame is arranged above the bottle storage platform; the bottle storage platform, the bottle feeding track and the push / pull mechanism are fixedly arranged on the frame in sequence from the inlet / out of the freeze dryer to the outside; the push / pull mechanism comprises a slide bar assembly which comprises a push / pull plate; the bottle storage frame moves up and down above the bottle storage platform and comprises a frame body covered on the penicillin bottle; the frame body is provided with a plurality of connecting parts; and one end of the push / pull plate is operably connected with the connecting parts. By adopting the device, entering and exiting processes can be finished with only one set of equipment, so that the equipment investment cost is saved. Contact of a person with a medicament is avoided, and the medicament is kept sanitary. The automatic one-time feeding amount is large, so that the production efficiency can be increased.

The invention discloses a mixed bacterial agent capable of degrading antibiotics in soil, belonging to the field of microbial technology. The mixed bacterial agent is prepared by mixing Bacillus subtilis J5P2 and Pseudomonas J2 according to a volume ratio of (0.1-3): 1. A preparation method for the mixed bacterial agent comprises the following steps: I, preparation of a suspension; II, colony culture: (1) preparation of a medium and (2) culture; III, separation of strains; IV, subculture and domestication: (1) domestication, (2) preparation of a LB medium, and (3) preservation; V, preparation of inoculum liquid; and VI, preparation of the mixed bacterial agent. The mixed bacterial agent provided by the invention has the characteristics of capacity of effectively degrading a plurality of residual antibiotics in soil and reducing environmental pollution, etc., and can be used for the remediation of land contaminated by antibiotics such as tetracycline, penicillin, sulfadiazines and quinolones.

The invention designs a prescription of a levetiracetam injection and a preparation method thereof. The prescription is mainly composed of levetiracetam as an active component as well as an acetate buffer solution, sodium chloride and water for injection. The preparation method of the prescription comprises the steps of: weighing the components, stirring for dissolving, adding 0.05%-0.2%(W / V) activated carbon for injection, performing pressure filtration on the liquor to remove pyrogen, regulating the pH value of the solution to be in a range of 5.0-6.0, performing coarse filtration with a 0.45mu m filter membrane, then performing fine filtration with a 0.22mu m filter membrane, filling into 5ml penicillin bottles after quality inspection pass, capping and sealing, and sterilizing under a terminal sterilizing condition of 121 DEG C for 15 minutes. The levetiracetam injection produced by the invention has a high sterility level, and the safety performance is more fully guaranteed for clinical medication.

The invention discloses an electrochemical biosensor for detecting penicillin and a preparation method and application thereof. The electrochemical biosensor is characterized in that a working electrode is a magnetic glassy carbon electrode immobilized with MWCNTs-Fe3O4 / Au-HRP-Ab. The preparation method comprises the following steps: carrying out carboxylation on a multi-walled carbon nanotube, preparing Fe3O4 / Au composite nano particles, preparing a Fe3O4 / Au magnetic nano particle-HRP-penicillin antibody, sequentially modifying the carbon nanotube subjected to carboxylation and Fe3O4 / Au-HRP-Ab to the surface of the glassy carbon electrode of the carbon nanotube, forming the electrochemical biosensor by taking a platinum electrode as a counter electrode and taking a saturated calomel electrode as a reference electrode, immersing the electrochemical biosensor into a sample to be detected, and determining the concentration of the penicillin in the sample to be detected according to a quantitative relation between a corresponding current value and the concentration of the penicillin. The electrochemical biosensor has the advantages of high sensitivity, high selectivity, high accuracy and high detection speed.

The invention relates to a serum-free adipose tissue-derived mesenchymal stem cell culture medium, which consists of a basic culture medium and added ingredients, wherein the basic culture medium is DMEM-LG, and the added ingredients and the content of each added ingredient are shown as follows: 5 to 20ng / mL of alkaline fiberblast growth factors, 5 to 20ng / mL of epidermal growth factors, 100U / mL of penicillin, 100 micrograms / mL of streptomycin, 50 to 200 micrograms / mL of heparin, 2 to 8mM of L-glutamine, 100 to 300 microM of 2-mercaptoethanol, 500 to 2000U / mL of leukaemia inhibitory factors and 0.5 to 2mM of sodium pyruvate. The serum-free adipose tissue-derived mesenchymal stem cell culture medium does not contain the serum, so that the inter-batch difference and the influence of the serum component on the cell culture can be avoided; the exogenous pollution of the serum and the toxicity of the serum on the cells can be avoided; and the ingredients are clear, so that the research of the psychological regulation mechanism of the cells can be facilitated.

The invention discloses a method for improving survival rate of triangular bream in long-distance transport. Anti-stress effect of vitamin C, sedative effect of low-concentration clove oil and antibacterial effect of penicillin sodium are applied, metabolism and stress reaction of fish fry in transportation process are effectively reduced, and injury and infection of the fish fry in the transportation process can be effectively prevented, so that the survival rate is improved.

The invention relates to a macropore carrier 'synchronization method' covalent crosslinking-immobilized papain polymer and a method. In the papain polymer, a papain is embedded in the pore canal of a carrier the aperture of which is more than 0.5mu m; an inorganic macroporous material or an organic macroporous material the surface of which is provided with hydroxyl is taken as an immobilized enzyme carrier; synchronous complementation of the formation of the crosslinking papain polymer and the covalent connection of the crosslinking papain polymer and the macroporous carrier is finally realized through amino-group modification, enzyme adsorption, enzyme precipitation and synchronous crosslinking on the surface of the carrier; the enzyme carrying amout of the immobilized enzyme is higher than that of the immobilized enzyme of a common carrier; the enzyme leakage caused by applications of the macroporous carrier is avoided by adopting a covalent fixed method; the carrier shape can be adjusted according to the actual requirements; and the optimum catalysis temperature, pH value, solvent and heat stability are obviously improved. The papain polymer provided by the invention can be applied to other proteases, lipase, amylase, glucose isomerase, penicillin, acylase, pectinase, oxidase, L-asparaginase, aspartase and peroxidase and the like.

The invention discloses a normal temperature preservation diluent for sperm, comprising 120ml to 140ml double distilled water, 1.0g to 3.0g fructose, 3.5g to 5.5g Tris, 2.0g to 3.5g citric acid, 30mg to 50mg GSH, 140mg to 180mg BS, 30mg to 50mg ATP, 450mg to 550mg VC, 300,000 to 500,000 penicillin, 150,000 to 250,000 streptomycin and 1.0g to 2.0g trehalose; the optimum operating temperature ranges from 15 DEG C to 25 DEG C. The normal temperature preservation diluent for sperm has the advantages that, the diluent has good effect on the normal temperature preservation of the sperm of cattle, sheep and deer; the invention has simple production method, low production cost and convenient popularization and application.

The present invention describes an assay method comprising: (A) generating (1) at least a first fragment of a reporter molecule linked to a first interacting domain and at least a second fragment of a reporter molecule linked to a second interacting domain, or (2) nucleic acid molecules that code for (A)(1) and subsequently allowing said nucleic acid molecules to produce their coded products; then, (B) allowing interaction of said domains; and (C) detecting reconstituted reporter molecule activity, where said reporter molecule can react with a penicillin- or a cephalosporin-class substrate.

The invention relates to novel oxazinones designed to bind to the penicillin receptor, methods of synthesizing the compounds, and the use of the compounds as antibacterial agents. The compounds have the general formula (I)Preferably the compounds have a carboxyethyl or a substituted carboxymethyl substituent at the 2-position and a hydroxyl group at the 5-position and have a molecular shape suitable for binding to and reacting with the active site of a pencillin-recognizing enzyme. The compounds are synthesized by condensing a carboxyl-protected N-hydroxy amino acid with a 3-hydroxyprotected-4-bromobutanoic acid to form a a doubly protected N-hydroxy N-acylamino acid, which is cyclized with an organic base to yield a doubly protected 1,2-oxazin-3-one. The protecting groups are then removed to provide an antibacterial agent.

The invention discloses a tissue sample preservative solution used for preserving tissue samples, such as fat, umbilical cords, placentas and the like, after collection and before separation. The tissue sample preservative solution mainly comprises following components: high-glucose DMEM dry-powder culture medium, sodium bicarbonate, DMSO, dexamethasone, insulin, penicillin, streptomycin, amphotericin and a heparin sodium injection. The high-glucose DMEM dry-powder culture medium and the sodium bicarbonate are used for maintaining osmotic equilibrium among cells inside and outside tissue, maintaining a pH value, maintaining a moistening situation of the tissue and providing nutritional components. The DMSO is a freeze-storage protective agent and can prevent freeze-injuries on the tissue samples. The dexamethasone can inhibit immunization and protect activity of stem cells in the tissue sample; the insulin can promote absorption and utilization of the tissue sample to glucose. The penicillin, the streptomycin and the amphotericin can prevent pollution from bacteria and moulds and can eliminate pollution which has occurred. The heparin sodium injection can prevent blood solidification in the tissue samples and increase a yield of the stem cells. The tissue sample preservative solution is simple in components, is low in cost, is convenient to use, can maintain activities of the stem cells in the tissue samples, such as fat, umbilical cords, placentas and the like, and can greatly reduce a time limit from collection to preparation of the tissue samples.

The invention discloses a limbal stem cell serum-free medium, consisting of the following raw materials (calculated by weight percent): DMEM / F12 60ml-90ml, BSA 0.5g-4g, EGF 1Mug-4Mug, insulin 0.1mg-1mg, hydrogen 20ug-100ug, cholera toxin 1ug-10ug, transferrin 0.1mg-1mg, selenious acid 0.1ug-1ug, GCLCM 10ml-40ml, penicillin 5*103IU-2*104IU and streptomycin 5*103IU-2*104IU. The invention adopts the method that the culture medium is added with fibroblast conditioned medium, and the nutritive factors are supplemented with the hormone and cytokine combination, fibroblast conditioned medium and BSA. As a result, the nutritive needs for long-time in-vitro expansion and cultivation of the limbal stem cell serum-free medium are satisfied, and the medium is serum-free and suitable for commercial production.

The invention discloses an anti-freezing agent and an anti-freezing diluent for freezing and storing livestock sperm and a preparation method thereof. The anti-freezing agent for freezing and storing bovine and porcine sperm is algin, the amount of the algin added into the conventional diluent is 60 to 110 milligrams, wherein the conventional diluent is prepared from 1.1 grams of glucose, 1.48 grams of citric acid, 2.42 grams of Tris, 10 percent of fresh egg yolk, 0.06 gram of penicillin sodium and 0.1 gram of streptomycin sulfate, which are dissolved in 100 millimeters of distilled water. The adding amount of the algin for cattle is 100 to 110 milligrams, and the adding amount of the algin for pigs is 60 to 80 milligrams. The preparation method comprises the following steps of: adding 100 to 110 milligrams of algin and 4 percent glycerin (for cattle) or 60 to 80 milligrams of algin and 1 percent glycerin (for pigs) into the conventional diluent to prepare the anti-freezing diluent; and adjusting the pH value of the solution to be between 6.5 and 7.5, filtering and sterilizing the anti-freezing diluent under the osmotic pressure of 286mOsm / L, and putting the anti-freezing diluent into a 4 DEG C refrigerator for later use. The algin is used for freezing and storage of the bovine and porcine sperm for the first time, and after unfreezing, the sperm motility rate, the acrosome completeness rate and the plasma membrane completeness rate are 65 percent, 69 percent and 72 percent (for cattle) respectively and 58 percent, 62 percent and 63 percent (for pigs) respectively.

The invention relates to a freezing diluent for a seminal fluid of livestock. Every 100 ml of the freezing diluent comprises components as follows: 2.71 g of trihydroxymethyl aminomethane, 1.4 g of citric acid, 1.0 g of monosaccharide, 5-20 ml of fresh yolk, 0-10 ml of a permeable protecting agent, 0.1 million IU of penicillin, 0.1 million IU of streptomycin, 0.05-5 g of polyvinyl alcohol and the balance of ultrapure water. The common high-molecular polymer polyvinyl alcohol serving as an ice crystal inhibitor replaces natural antifreeze protein and is applied to the cryopreservation research of the seminal fluid of livestock for the first time. The survival rate of unfrozen sperms is about 75%, the activity is 50% or higher, the acrosome completeness is 65% or higher, the plasma membrane completeness is about 50%, and the non-return rate after artificial insemination is 70% or higher.

The invention relates to an equus semen storage diluter and a preparing method and a use method thereof, belonging to the technical field of animal semen storage. The equus semen storage diluter is prepared from double distilled water, glucose, glycin, penicillin, streptomycin and yolk. The equus semen storage diluter has a good effect of preserving the equus semen under the normal or low temperature, has the advantages of simple operation, low cost, easy mastery, and is suitable for popularization and application, which is beneficial to the development of the equus breeding.

The invention discloses an automatic medicine-dispensing device. The device comprises an input module, a bottle-opening module, an interactive module, a solvent module, a peristaltic pump module, a dustbin module, a lifting window component and a control module, wherein the input module, the bottle-opening module, the interactive module, the solvent module and the peristaltic pump module are arranged in a medicine-dispensing cavity located in the upper portion of the automatic medicine-dispensing device, and the dustbin module and the lifting window component are arranged in a support cavity located below the medicine-dispensing cavity. Through the automatic medicine-dispensing device, the liquid injection of a penicillin bottle, the dissolution of medicine in the penicillin bottle, opening of an ampoule bottle and the suction of medicine liquid can be automatically achieved, after medicine dispensing is completed, discarded medicine bottles and needles are discarded in a dustbin, and therefore the whole medicine-dispensing process is automatically completed. The automatic medicine-dispensing device improves the efficiency of medicine dispensing, can avoid medicine liquid pollution easily caused by manual operation at the same time, and improve safety.

The invention relates to the technical field of biological control of breast cancer disease and discloses a polypeptide for promoting apoptosis of breast cancer cells by targeted uptake of siRNA. Thepolypeptide comprises a polypeptide 1; the sequence of the polypeptide 1 is H-Ile-Phe-D-Trp-Leu-Leu-Trp-Gln-Gly-Arg-Gly-Gly-Gly-Arg-Arg-Arg-Arg-Arg-Arg-Arg-OH. A detection method for the polypeptide for promoting the apoptosis of the breast cancer cells by targeted uptake of the siRNA comprises the following steps: firstly, culturing the breast cancer cells: selecting breast cancer cells MDA-MB-231, culturing the breast cancer cells MDA-MB-231 by adopting a DMEM culture medium, and sequentially adding 10 percent of fetal calf serum, 100 unit / ml of penicillin and 100g / mL of streptomycin in theculture medium. According to the polypeptide for promoting the apoptosis of the breast cancer cells by targeted uptake of the siRNA, the polypeptide 1 wraps the siRNA to form nanoparticles for targeting delivery of the siRNA to the breast cancer cells; the nanoparticles can realize the targeting delivery of the siRNA through a surface receptor of the breast cancer cells and have little damage to normal cells, and the practicality of the polypeptide is improved; meanwhile, the targeting delivery of TRPC1 siRNA by using the polypeptide 1 causes the apoptosis of the breast cancer cells, so that the effect of treating breast cancer is realized.

The invention relates to a diluent for storing the sperm of a pig under normal temperature, which is characterized by comprises the following components by weight ratio: 9-11 of glucose, 0.2-0.3 of ethylene diamine tetraacetic acid, 0.5-0.6 of trisodium citrate, 0.08-0.12 of 1570U / mg penicillin and 0.2-0.25 of 720U / mg streptomycin. The diluent is completely dissolved in distilled water with distilled water according to the dilution ratio that diluent of 1g is diluted with distilled water of 20ml, and dilution liquid with the pH of being 6.5-6.8 and the osmotic pressure of being 290-310mOsmo / L is obtained; the diluent of the sperm of the pig, produced by the formulation, can effectively prolong the holding time, has convenient use and low cost, is suitable for being used by an artificial insemination station of the pig and a large-scale pig farm, has good application effect in the long-term production, has wider application range than the imported sperm, and can lead the conception rate of a sow in the oestrous period when being applied to a foreign pig to enable conception rate of a sow to be up to 86.8 percent with the litter size of 11.2 heads.

The invention relates to the technical field of a biosensor, and particularly relates to an aptamer-based biosensor for detecting penbritin, which is used for solving the problems of low specificity and sensitivity in the traditional method for detecting the penbritin in the prior art. The aptamer-based biosensor for detecting the penbritin is characterized in that a capture probe layer, HAP and an MB probe layer are sequentially decorated on an electrode. The preparation method comprises the following steps: preprocessing the electrode; decorating the capture probe layer onto the surface of the electrode; decorating the HAP and the MB probe layer onto the surface of the electrode. By utilizing the specificity recognition of aptamer, the aptamer of penbritin is used as a recognition substance, so that the high-specificity detection on the target penbritin is realized; by utilizing the polymerization effect of polymerase, the cycling utilization of a target substance is realized, and an effect on amplifying the signal is realized.

A Bacillus strain characterized by (i): sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol; (ii) antimicrobial activity against E. coli and Clostridium perfringens; and (iii) a sporulation percentage of at least 80 when measured after 2 days of incubation. The invention further relates to a method for selecting such strains. Many of the identified strains according to the invention are of the species Bacillus amyloliquefaciens. Some of the Bacillus amyloliquefaciens were further identified as Bacillus amyloliquefaciens subsp. amyloliquefaciens whereas others were identified as amyloliquefaciens subsp. plantarum. A Bacillus strain of the invention may be used as a feed additive to animal feed where it has a probiotic effect.

The invention relates to an HUVEC (human umbilical vein endothelial cell) separation, culture and subculture method belonging to the technical field of biology. The method comprises the following steps: (1) separation of HUVEC: taking a fresh umbilical cord, cleaning with a sterile PBS (phosphate buffer solution) containing penicillin-streptomycin, adding collagenase to perform water bath digestion at 37 DEG C, centrifuging the digestion solution, then adding an M199 culture medium, transferring into a gelatin-coated culture bottle, and culturing in a CO2 incubator at 37 DEG C; (2) culture of HUVEC: after the HUVEC is cultured at 37 DEG C for 24 hours, pouring out the culture medium, cleaning with the PBS, adding a fresh M199 culture medium, and afterwards, changing the culture medium once every two days, wherein the HUVEC can be subcultured generally after being cultured for 5-7 days; and (3) subculture of HUVEC: pouring out the culture medium, cleaning with the PBS, adding the digestion solution to digest the cell, adding a DMEM (dulbecco's modified eagle medium) containing serum to terminate the reaction once the cell is rounded, centrifuging the digested cell, and adding a fresh culture medium, wherein the HUVEC subcultured for 2-3 generations is used for experiments. The separated HUVEC is economical and practical, is simple and easy to use, and is beneficial to obtaining required in-vitro experimental model cells.