The invention belongs to the technical field of medicines, and discloses a delivery system based on protein-phospholipid. The system comprises a protein-phospholipid dispersion with the weight ratio of between 1:100 and 100:1, wherein the grain size of the protein-phospholipid is between 5 and 1,000nm, and the surface of the phospholipid or the surface and the inside of the phospholipid are coated / covered by a protein layer. The preparation method comprises: preparing a phospholipid containing phase, preparing a protein containing water phase, performing the homogenization treatment on the phospholipid containing phase and the protein containing water phase at a temperature of between 0 and 40 DEG C and under a pressure of between 400 and 40,000psi, and obtaining a dispersion system with the average grain size of less than 1,000nm. The delivery system has the advantages of realizing the delivery in various ways such as injection, oral taking, cavities and canals, skin and the like, and lowering side effects of medicines. The delivery system solves the problems of drug-loading rate and stability of protein nano-grain and liposome, and adopts low-toxicity organic solvent, even no organic solvent; and the preparation method is easy for industrialized application, and can prepare a liquid type protein-phospholipid containing delivery system.