4926results about How to "Keep alive" patented technology

A biologic deodoring and cleaning agent for treating the refuse to prepare fertilizer and cleaning and activating water area contains high-safety yeast, lactobacillus, nitrogen-fixing bacteria, functional bacillus, and photosynthetic bacteria.

The invention discloses a liquid nitrogen cylinder, a cryogenic vial storing and fetching device and a liquid nitrogen cylinder storing and fetching system. The liquid nitrogen cylinder comprises a cylinder body, wherein the cylinder body is provided with a sealed opening; the liquid nitrogen cylinder further comprises a bracket tray; the bracket tray is arranged in the cylinder body; the bracket tray is used for bearing cryogenic baskets; a central shaft is vertical to the bracket tray and is fixedly connected with the bracket tray; a driving component is electrically connected with the central shaft; the driving component is used for driving the central shaft to rotate; and the central shaft is used for driving the cryogenic baskets to rotate through the bracket tray and conveying the cryogenic baskets to the opening. By adopting the liquid nitrogen cylinder, the error rate of manual cryogenic vial selecting is reduced; and meanwhile, safety hazards for operation personnel during a manual bracket tray rotation process are avoided. By adopting the cryogenic vial storing and fetching device, the process for selecting a single target cryogenic vial for biological samples is realized; and during the whole selecting process, the cryogenic vials are all in a liquid nitrogen environment, so that non-target cryogenic vials are prevented from being exposed at environment temperature. The liquid nitrogen cylinder storing and fetching system is applicable to vial picking work for large biological sample banks.

The invention belongs to the technical field of molecular sieve preparation, and particularly relates to an in-situ preparation method for a microporous-mesoporous molecular sieve containing noble metal. The in-situ preparation method comprises the steps as follows: adding a coupling pore-forming agent, a silicon source, an aluminum source or a titanium source, and an alkali source into a water solution of noble metal nano particles in sequence under the water bath condition; and ageing, drying, crystallizing, drying and carrying out high-temperature calcination to obtain the microporous-mesoporous molecular sieve containing the noble metal. The prepared microporous-mesoporous molecular sieve is provided with a hierarchical pore structure; the noble metal nano particles with high dispersity are covered in situ while a mesoporous structure is generated; and a synthetic method is convenient and simple, saves energy and reduces emission. A multifunctional catalyst prepared with the method integrates the advantages of the microporous channels of the molecular sieve, the transgranular meso pores and the intergranular meso pores of the molecular sieve and the noble metal nano particles, and is more suitable for catalytic reactions of sulfur-containing large molecules such as hydrogen desulfurization and the like.

The invention provides a silicon-based composite material, which is prepared from silicon particles, silicate and optional carbon, wherein the mixture of the silicate and the optional carbon forms a massive body, and the silicon particles are dispersed in the massive body. A preparation method comprises the following steps of: dispersing the silicon particles into absolute ethyl alcohol and / or deionized water to form suspension liquid; dispersing the silicate and the optional carbon into the absolute ethyl alcohol and / or the deionized water to form suspension liquid; ultrasonically oscillating the two kinds of suspension liquid respectively, and then stirring; dropwise adding the suspension liquid of the silicon particles into the suspension liquid of the silicate and the optional carbon to form mixed liquid, heating and stirring the mixed liquid until evaporating the mixed liquid into paste; then, putting the paste in an oven to be dried to obtain masses, and grinding and sieving to obtain undersize particles; and conducting heat treatment in an inert atmosphere, and grinding and sieving to obtain the silicon-based composite material. According to the silicon-based composite material, a lattice structure of the silicon particles can be ensured, therefore the activity of the silicon particles is ensured, and the energy density, the first-time coulomb efficiency and the high-temperature storage performance of lithium ion batteries are increased.

The invention relates to a communication fuel cell spare power supply system, which comprises a hydrogen generation and storage unit, a fuel cell unit, a DC / DC unit, an output unit, an electric control unit, a routing inspection unit, a monitoring unit and a communication unit, and is characterized in that: the hydrogen generation and storage unit generates hydrogen by using solar or wind energy and releases the hydrogen by absorbing the heat of the fuel cell unit; the fuel cell unit generates direct current electric energy and heat by the electrochemical reaction of the hydrogen and oxygen; the DC / DC unit adjusts and boosts the direct current electric energy and then supplies the direct current electric energy to the output unit; the output unit supplies power to a load when commercial power fails; the electric control unit acquires various data and sends control information to all units; the routing inspection unit acquires all individual cell voltage values for transmission; the monitoring unit displays various parameters and working states and realizes human-computer interaction; and the communication unit performs short-range and long-range communication and monitoring. The power supply system is clean, high efficient, reliable and applicable to various communication spare power supplies.

The invention relates to green safe feed for animals in the field of animal husbandry and veterinary medicine, in particular relates to a recipe and a production process of the feed. Green safe feed for fleshy poultry is mainly characterized by comprising the following raw materials: corn, wheat, bean pulp, corn protein powder, cotton seed meal, mixed oil of bean oil and poultry oil, common salt,calcium hydrophosphate, mountain flour, lysine, methionine, mineral feed additives, choline chloride, composite vitamins, coated sodium butyrate, composite Chinese herbal medicine feed additives, emulsifying agents, antioxidants and mycotoxin absorbing agents. The green safe feed has the advantages that the current conditions of high medicine residue content in the poultry meat and poor flavor caused by the abuse of various antibiotics and chemical additives in broiler raising are changed, the feed cost is saved, the economic benefits are improved, and the sustainable development of the animal husbandry is promoted.

The invention discloses a liquid nitrogen container and a cryogenic vial pick-and-place device. The liquid nitrogen container comprises a container body, and further comprises a rotary disc, a cryopreservation frame, a central shaft and a driving assembly, wherein an opening is formed in the container body; the rotary disc is arranged in the container body; the cryopreservation frame is supported by the rotary disc and comprises a plurality of layers of placement faces; the included angle between each placement face layer and the rotary disc is 40-60 degrees; the central shaft is perpendicularly arranged with and fixedly connected with the rotary disc; the driving assembly is electrically connected with the central shaft and used for driving the central shaft to rotate; and the central shaft is used for driving the cryopreservation frame to rotate through the rotary disc to reach the opening of the container body. By adoption of the liquid nitrogen container and the cryogenic vial pick-and-place device, the workload of operating personnel is reduced, the error rate of manual picking of cryogenic vials is lowered, and the potential safety hazard for the operating personnel is avoided. The liquid nitrogen container and the cryogenic vial pick-and-place device are particularly suitable for the work of picking cryogenic vials in a high-capacity biological sample bank, and meet the technical requirement for storage in the high-capacity biological sample bank.

The invention relates to a soil conditioner, in particular to a compound water retention soil conditioner and preparation thereof. The soil conditioner comprises the components by weight parts: 100 to 200 parts of organic matter, 0.2 to 3 parts of active cover agent, 150 to 200 parts of acrylamide, 50 to 100 parts of starch, 20 to 50 parts of attapulgite, 20 to 40 parts of porous inorganic matter, 30 to 60 parts of clay, 0.5 to 2 parts of microorganism microbial inoculum, 100 to 150 parts of acid liquid, 2 to 4 parts of initiator, 0.5 to 1 part of crosslinking agent, 1 to 5 parts of urea and 0.03 to 1 portion of yeast. The compound soil conditioner reserves the advantage of water retention; at the same time, because equivalent organic materials, the porous inorganic matter, the clay and the like are added to the soil conditioner, the soil conditioner has the functions of modifying soil structure, enhancing the organic matter of soil and improving the pH value of the soil; and because humic acid is added, the soil conditioner has the function of a urease inhibitor to a certain degree and enhances the utilization efficiency of a fertilizer N applied together with the soil conditioner.

The invention relates to preparation and application of a biological carbon immobilized microbial agent. The method comprises the following steps of: sequentially performing seed solution culture and liquid fermentation culture to microorganisms capable of degrading petroleum to obtain fermentation liquid; performing mixed adsorption to the fermentation liquid and biological carbon to prepare the biological carbon immobilized microbial agent. The invention further provides application of the biological carbon immobilized microbial agent to soil polluted by oily sludge and sand and petroleum. By immobilizing the microorganisms having a function of degrading petroleum and then remedying the soil polluted by oily sludge and sand and petroleum, the petroleum removal efficiency is remarkably improved and the removal rate is improved by 20 to 30 percent as compared with remediation by solely using microorganisms. The invention uses agricultural wastes such as corncobs as precursors to prepare carriers, the cost is low, a great amount of microbial agent is easily obtained and simultaneously the reclamation of wastes is realized.

The invention provides an intestinal tract micro-ecological preparation and its preparing process, wherein each 100g of the product contains bifidobacteria 1.0-20.0g, Lactobacillus acidophilus 1.0-25.0g, Streptococcus thermophilus 1.0-25.0g, Bulgarian subspecies of Lactobacillus fermentum 1.0-25.0g, saccharomycete 1.0-35.0g, and right amount of isomaltose hypgather, fructooligosaccharide, and many kinds of vitamins.

The invention provides a preparation method of high-purity collagen protein sponge, and relates to a preparation method of collagen protein sponge. The preparation method of the high-purity collagen protein sponge is used for solving the problems that the collagen protein sponge prepared by using a conventional method is long in production cycle and low in yield and purity and has poor hemostasis performance. The preparation method of the high-purity collagen protein sponge comprises the following steps: step one. pretreating fresh bovine heel tendons; step two. extracting collagen protein; step three. centrifuging; step four. salting out; step five. dissolving; step six. carrying out gradient dialysis; step seven. pre-freezing; step eight. carrying out freeze drying; and step nine. sterilizing. The final product prepared by using the method has a smooth and flat surface and relatively good hemostatic performance and is uniform in pore size distribution. The product has relatively high purity (the total amount of amino acids reaches 97.73%), an obvious hemostatic effect and no abnormal taste, is safe, non-toxic, high in yield and short in time; liquid is clear without impurities; the production cycle is shortened; the whole preparation process is carried out at a room temperature; the biological activity of the collagen protein is maintained; and the application of the high-purity collagen protein sponge in clinical is improved.

The invention relates to the field of stem cells, discloses a method for preparing exosome freeze-dried powder of stem cells, and particularly relates to a method for preparing exosome freeze-dried powder of human amniotic mesenchymal stem cells. The method for preparing the exosome freeze-dried powder of the human amniotic mesenchymal stem cells disclosed by the invention comprises the following steps: mixing trehalose with exosomes of the human amniotic mesenchymal stem cells as a cryoprotectant; filtering and removing bacteria by virtue of a filter membrane; and firstly carrying out ultralow temperature pre-freezing, and then carrying out low-temperature freezing at a certain vacuum degree. The preparation method disclosed by the invention is simple to operate, safe and effective. An experiment proves that compared with a conventional preparation method of the freeze-dried powder, the morphologies and the activity of the exosomes of the human amniotic mesenchymal stem cells can be well kept by the method for preparing the exosome freeze-dried powder of the human amniotic mesenchymal stem cells disclosed by the invention; and the method is suitable for long-term preservation and application of the exosomes of the human amniotic mesenchymal stem cells.

The invention provides a preparation method of chloroepoxy propane by chloropropene epoxidation. Chloropropene, a solvent and titanium silicalite molecular sieve catalyst fine particles are put in a reaction kettle for reaction at the temperature of 10-80 DEG C. The method is characterized in that the reaction kettle is a single kettle or multistage slurry bed reaction kettles with same or different volumes which are connected in series; the reaction solution needs not to be homogeneous phase, slurry particles of the chloropropene, the solvent and the titanium silicalite molecular sieve catalyst are added to a first stage reaction kettle by a metering pump, hydrogen peroxide is dropwise added to the first stage reaction kettle or each stage of the reaction kettles, the reaction occurs in a weakly acidic condition, materials are subject to oil-water delamination after the reaction to be divided into oil-phase slurry and water-phase slurry; the catalyst is suspended in the water phase, and the oil phase is sent to a rectification tower to separate the chloropropene, the epichlorohydrin and trace methanol; the water phase slurry is filtered, a catalyst filter cake or concentrated slurry is directly beaten by the solvent, and pumped to a first stage epoxidation reaction kettle, the water phase slurry contains the methanol, trace epichlorohydrin and the water which is generated by the reaction and the water which is taken by the hydrogen peroxide, the trace methanol and the epichlorohydrin are separated out by negative pressure rectification, and the recovered methanol is used for preparation of the catalyst slurry. The method has the advantages of simple process, little solvent consumption and high product separation yield, and is available for industrialized production.

The invention provides a bioremediation composition for in situ bioremediation of soil and / or groundwater contaminated with hydrocarbons, comprising an adsorbent capable of adsorbing said hydrocarbons, a mixture of facultative anaerobes capable of metabolizing said hydrocarbon under sulfate-reduction conditions, a sulfate-containing compound that releases sulfate over a period of time, and a nutrient system for promoting growth of said anaerobes, wherein said nutrient system includes a sulfide scavenging agent.

The invention discloses an application of nano-gold directly bonded with luminol in an immunoassay. The invention is characterized in that the immunoassay probe of a nano-gold directly bonded with luminol comprises antibodies which are labeled by the nano-golds directly bonded with luminol, wherein the nano-gold directly bonded with luminol are prepared through reducing chloroauric acid by the luminol at a single step; a chemiluminescence immunoassay method is based on the immunoassay probe of the nano-gold directly bonded with luminol; and a kit is used for carrying out the immunoassay method. The chemiluminescence immunoassay method of the invention has the advantages of high sensitivity (for instance, the detection limit can reach 1.0pg / mL for detecting human IgG), wide range of linearity, good repeatability, simple operation, low cost and the like, can be applied to detect multi-analyte in various samples and has key application prospect in fields, such as clinical diagnosis and cure, drug analysis, food security detection, environmental monitoring and the like.

The invention provides a tissue engineering nerve graft based on a biological printing technology and a preparation method thereof. The tissue engineering nerve graft comprises an outer tube and a tube inside scaffolds, wherein trophic factors and / or cells can cover the inner surface and the outer surface. A high-fidelity printing operation is performed according to the actual nerve shape demand by utilizing the biological printing technology, a polymer solution is printed into the specified nerve graft in a three-dimensional way by using an ink-jet printer by adjusting the sizes and the quantity of the jet nozzles of the ink-jet printer, the distances from the jet nozzles to a bottom layer and the pulse frequency of a supercharger and writing a control program of specific printing, and the specified nerve graft is applied to treatment of peripheral nerve defect and spinal cord injury.

The invention discloses piggy creep feed, which mainly comprises corn, rice, full cream puffing soybean, fermentation bean pulp, peeled bean pulp, fish meal and other raw materials, wherein a yeast cell extractive is added to improve the immunity of the piggy, the complex enzyme preparation and probiotics replace the high copper and high zinc, so the pollution to the environment is reduced, meanwhile, the digestion and absorption of the feed are improved, and the normal flora of the intestinal canal is kept. The full organic trace element replaces the inorganic trace element, so the oxidation of the salt to the feed and the organism is reduced. The invention further discloses a method for preparing the creep feed, which utilizes the granulation process, the objectionable constituents and the beneficial constituents are effectively accepted or rejected, so the nutrient value of the feed is effectively maintained.

The invention discloses a probiotic ultralow temperature refrigeration technology, a formula of a total-salt cryoprotectant which is suitable for the probiotic ultralow temperature refrigeration technology, and probiotic ultralow temperature refrigeration particles prepared by using the probiotic ultralow temperature refrigeration technology. The probiotic ultralow temperature refrigeration technology comprises following steps: (1) preparing a fermentation medium; (2) culturing probiotics; (3) centrifuging; (4) preparing the cryoprotectant; (5) adding the cryoprotectant; and (6) quick-freezing and moulding. The invention also provides a probiotic preparation prepared by using the probiotic ultralow temperature refrigeration particles, and a preparation method of the probiotic preparation. The probiotic ultralow temperature refrigeration particles are probiotic refrigerated bacterial strains which do not need to be dried and possesses advantages of fast refrigeration speed, high living bacterial rate and excellent rehydration performance. The probiotic ultralow temperature refrigeration particles contain water, can be dissolved in solutions more quickly, and are capable of preventing probiotic cells from damage caused by rehydration of normal low temperature refrigeration dried probiotic powder, and increasing the living bacterial rate of the probiotic preparation greatly.

The invention discloses a method for preparing an immuno biosensor for measuring ractopamine (RAC) in the technical field of chemical detection. Nanogold, a carbon nano tube having conjugated bonding coupled RAC-bovine serum albumin, and prussian blue are modified on a glassy carbon electrode sequentially to obtain the electrochemical immuno biosensor. By the method, the electrochemical immuno biosensor which has high sensitivity, high stability and can quickly test the ractopamine can be prepared.

The invention provides a face verification anti-counterfeit recognition method and a system thereof based on an interactive action. The method comprises a step of carrying out the initial recording of the information of an register static face image and the information multiple register face action images, a step of waiting the reading of a static face image to be read, matching a character and a stored character when the shooting of static face image to be detected obtained by a user to be verified is detected, and if a matching degree reaches the storage characteristic of a preset threshold value, conforming to a verification requirement, and a step of randomly selecting and prompting the user to be verified to complete a corresponding face action according to the recorded face action, extracting the characteristic of the action image to be detected of the user be verified, matching the characteristic with the historical verification feature information of a corresponding face action, completing face identity verification if a matching rate reaches a preset threshold value, adding the matched image into the historical verification feature information, returning to select a next face action to continue matching if the matching is not approved or does not reaches a desired effect, treating the verification as a failure if the action exceeds a preset number of times, and ending the verification.

The invention relates to a method for extracting active polysaccharides from brown algae. The method comprises the following specific steps of: putting a cleaned, dried and smashed alga raw material into an extraction container, and adding an organic acid solution or organic-inorganic mixed acid solution; heating a stirred and wetted material to 50-150 DEG C to realize partial degradation of highpolymer polysaccharide substances of brown algae; fully washing with an organic solvent till the acid solution in the material is discharged completely; adding 5-8 times of volume of water for extracting, concentrating an extracting solution, regulating the PH value to neutral, and gradually depositing in alcohol to obtain align, fucoidin and laminaran which are rich in mannuronic acid fragments (rich in M) respectively; and pretreating brown alga raw material residues with water-extracted organic acid according to an alkali digestion process to obtain align which is rich in guluronic acid fragments (rich in G). By adopting the method, the defects of high water consumption, severe pollution and the like existing in the conventional align production process are overcome; and the method has the advantages of saving in energy, reduction in emission, high product yield, small extraction solvent dosage, and the like.

This invention discloses one photoelectric reactor to degrade organic pollute and one method to remove the pollute assistant with catalyze reactor, wherein, the reactor is located with conductive multi-hole materials load compound semi-conductor photo catalyze positive and negative electrodes; the optimized catalyze can be used as light source; it uses conductive solid electrolyte applied to degrade pollute with gas and liquid and to separate metal ions.

The invention discloses a Byzantine fault-tolerant alliance chain consensus method and system for power consumption information protection and a storage medium, and the method comprises the steps thata blockchain node credit model is built, and a node with the highest credit is selected as a main node through the blockchain node credit model; the transaction initiator broadcasts transactions andpackages the transaction requests into blocks; the main node generates a proposal message from the block and signs the proposal message; after the slave node receives the proposal information, the legality of the proposal is verified, when the verification result is illegal, recording is carried out, the consensus is given up, and a view replacement protocol is initiated; the verified slave node enters a confirmation stage, executes the block transaction and replies a message to the client; and consensus is reached according to the node reply message condition received by the client, and eachnode executes block chaining. According to the method, the probability that the Byzantine node is the main node is reduced, so that the view replacement frequency is greatly reduced, and the system performance is effectively improved.

The invention relates to a preparation method of a shell core fiber covered endovascular stent graft. The steps are as follows: firstly, a polymer is dissolved in adaptive dissolvent to obtain solution with a certain concentration; secondly, medicine or an artificial polymer and medicine, and a bio-active element are dissolved in the adaptive dissolvent to obtain solution or suspension liquid; thirdly, the solution of the polymer and the medicine or the solution or suspension liquid of the medicine or bio-active element are respectively filled in two injectors, the speed of a micro injection pump, the voltage of an electrostatic generator and the distance of a receiving device are adjusted, fiber is obtained through the static spinning preparation, and the fiber is received as a tube shape or film shape structure; fourthly, the endovascular stent graft is fixed on a revolution axle, through the revolution of the endovascular stent graft, the static spinning fiber are directly received as fibrous membrane covered on the endovascular stent graft. The invention can effectively prevent smooth muscle cells from hyperplasia in the stent graft or from narrowing in the stent graft caused by the other functions, and the shell core fiber for the medicine loading can slowly release the medicine to attain the purpose of curing.

The invention relates to the field of cell culture, in particular to cell cryopreservation liquid, application thereof and a cryopreservation method of megakaryocyte progenitor cells. The cell cryopreservation liquid comprises DMSO, fetal calf serum, dextran, trehalose and albumin. By means of the cell cryopreservation liquid, the cytoactive of the megakaryocyte progenitor cells can be well maintained during the cryopreservation period, and damage to cells in the cryopreservation and resuscitation processes is lowered. According to the cell cryopreservation liquid, application thereof and the cryopreservation method of the megakaryocyte progenitor cells, the cryopreservation and temperature reduction processes are softer, the cells are stored in liquid nitrogen after the temperature reduction process is conducted, the influence on the cytoactive is small, and damage to the cells in the cryopreservation process is lowered. It is indicated by experiments that the vigour of the resuscitated cells can reach 94% one month after the megakaryocyte progenitor cells are cryopreservated in the cryopreservation liquid, and multiplication activity is good, and the cell cryopreservation liquid is significantly superior to the prior art.

The present invention relates to a preparation of fiber and its fabric containing heparin and bioactive molecule and its application. Its preparation method includes the following steps: dissolving heparin or heparin and bioactive molecule composite and polymer together, electrostatic spinning to prepare nano / micron fiber or coaxial cospinning to prepare shell-core fiber. The obtained fiber has good modality, and can be easily made into film-like fabric or tubular fabric.

The invention relates to a micromolecule walnut peptide and a preparation method thereof. The preparation method sequentially comprises the following steps of protein extraction, twice enzymolysis, filtering, purification, concentration and drying. In the twice enzymolysis process, alkaline protease with the mass being 1-3% that of the walnut protein is added into a walnut protein extracting solution for once enzymolysis; enzyme deactivation is performed on a once enzymolysis solution after the once enzymolysis is finished; one or more kinds of proteinase including the flavored proteinase, the neutral proteinase and the papain with the mass being 1-3% that of the walnut protein are added after the once enzymolysis solution obtained after enzyme deactivation is performed is cooled for secondary enzymolysis. According to the preparation method, the twice enzymolysis method is adopted, due to the specificity of the structure of the walnut protein structure, sufficient enzymolysis cannot be performed on the walnut protein through enzymolysis of one proteinase, and the twice enzymolysis method is adopted for ensuring that the enzymolysis is brought into play on the most suitable condition of each proteinase, and therefore the obtained walnut peptide content is high.

The invention discloses a green nursery stock planting substrate multifunctional additive, which is characterized in that the additive comprises the following components in weight portion: 1)150 to 200 portions of vegetable carbide; 2) 5 to 10 portions of polyacrylamide type high absorbent resin;3) 0.5 to 1 portion of inorganic fertilizer or compound fertilizer containing nitrogen, phosphorous, kalium elements and the like; 4) 0.05 to 0.1 portion of encapsulated type slow / controlled release fertilizer; 5) 10 to 20 portions of organic fertilizer; and 6) 0.03 to 0.05 portion of compound microbial inoculum comprising photosynthetic bacteria, actinomycete, microzyme, lactobacillus, rhizobium, bacillus and thiobacillus. By the additive, the planting survival rate of nursery stock is more than 98 percent, and the additive also has the advantages of quick seedling recovery, vigorous rooting, vigorous growth and obvious effects.

The invention discloses a macromolecular microcarrier and a preparation method thereof, belonging to the technical field of biomedicine. The preparation method comprises the following steps of: splitting a silk solution and a drug solution into micrometer grade liquid drops with core-shell structures under the action of a high-voltage electric field by adopting a coaxial high-voltage electrostatic technology and a freeze drying method; concreting through liquid nitrogen, and then preparing the microcarrier which is difficult to dissolve in water through freeze drying. The microcarrier is in the shape of a microsphere with the core-shell structure, and the diameter of the microsphere is 100-500 micrometers; the shell of the microcarrier comprises the components of silk fibroin; the random-coil conformation of silk fibroin molecules is 80-90 percent; the shell is in a porous structure, and an aperture is 5-20 micrometers; and a core of the microcarrier comprises the components of water-soluble drugs. The silk microcarrier has extensive application prospect in the fields of cell culture, drug release, and the like.