89 results about "Nerve graft" patented technology

The invention relates to a surgical system for repairing severed nerve ends. The severed nerve ends may be located on a single nerve or may be located on a nerve graft and a severed nerve. The surgical system includes a suture having a needle and an expanded tail, a clip, and a retaining member. Methods of repairing severed nerve ends and methods of adjoining severed nerve ends are also disclosed.

The present invention is a natural, cell-free tissue replacement that does not require difficult or extensive preparation made by washing tissue replacement in a solution including one or more sulfobetaines and an anionic surface-active detergent and washing the tissue replacement in serial solutions of the buffered salt to remove excess detergent. The natural, cell-free tissue replacement may be a nerve graft that supports axonal regeneration, guides the axons toward the distal nerve end and / or is immunologically tolerated. Other forms of the invention are a composition and kit prepared by the method of making a native, cell-free tissue replacement. The present invention may be modified for use in diagnostic, therapeutic, and prophylactic applications.

The invention discloses an artificial nerve graft prepared by electrostatic spinning and a preparation method and a special device thereof, and a product is a catheter-shaped nerve graft which is prepared from polymers by an electrostatic spinning method and consists of nano-fibers. The used materials of the product are all degradable materials and have good biocompatibility with human bodies. Theprepared product does not contain heterotoxin or side-effect substances introduced by a preparation process. A three dimensional structure with plentiful micropores is arranged on a conduit wall, provides a necessary approach for transporting nutritive substances needed in the growth process of nerve cells, has good use effect and provides a necessary induction function and a necessary growing space for the growth of the nerve cells.

The invention provides a tissue engineering nerve graft based on a biological printing technology and a preparation method thereof. The tissue engineering nerve graft comprises an outer tube and a tube inside scaffolds, wherein trophic factors and / or cells can cover the inner surface and the outer surface. A high-fidelity printing operation is performed according to the actual nerve shape demand by utilizing the biological printing technology, a polymer solution is printed into the specified nerve graft in a three-dimensional way by using an ink-jet printer by adjusting the sizes and the quantity of the jet nozzles of the ink-jet printer, the distances from the jet nozzles to a bottom layer and the pulse frequency of a supercharger and writing a control program of specific printing, and the specified nerve graft is applied to treatment of peripheral nerve defect and spinal cord injury.

The subject invention pertains to compositions and methods for culturing nerve tissue in vitro and nerve grafts produced using such methods. The compositions and methods of the subject invention can be employed to restore the continuity of nerve interrupted by disease, traumatic events or surgical procedures. The invention also concerns methods for promoting repair of damaged nerve tissue using the present compositions and nerve tissue treated according to such methods.

The invention discloses a chitosan artificial nerve graft containing a neurotrophic factor and a preparation method thereof. In the chitosan artificial nerve graft, chitosan is processed into a nerve conduit with a porous structure and high tensile strength by performing processes such as weak acid dissolving, injection molding, molding, neutralization fixing, cleaning, freeze drying and the like under the condition of not adding any foaming agent or crosslinking agent. The nerve conduit contains cells or tissues which have treating effects and are included or distributed inside or on the surfaces of tubular body pores, including autologous bone marrow stem cells, autologous bone mesenchymal stem cells, schwann cells or dorsal root ganglion tissues or combinations thereof. A tissue engineering nerve can be used for repairing peripheral nerve defect and can be applied to repairing of spinal cord injury simultaneously.

The invention relates to a bionic nerve graft used for peripheral nervous recovery and a preparation method and application thereof. The bionic nerve graft comprises a chitosan film conduit and a fibrous protein hydrogel beam positioned in the chitosan film conduit, wherein the fibrous protein hydrogel beam is prepared from fibrous protein through an electrospinning technique. The bionic nerve graft prepared by the preparation method is formed by the filling of the chitosan film conduit and the fibrous protein hydrogel beam of a multi-stage orientation structure, wherein a nerve regrowth space is effectively supported by a chitosan film, the orientational regeneration of a nerve axon is effectively guided by the fibrous protein hydrogel beam of multi-stage orientation, the cell affinity and the mechanical property of whole materials are good, and in addition, degrading speed and the repairing speed of peripheral nervous tissues are matched.

A medical nerve-grafting object containing chitin is prepared through preparing the cathether containing chitin from chitosan, preparing the chitin fibre supporter from chitosan, and embedding said supporter in said cathether. Its advantages are high effect, high and controllable degradability and high biocompatibility.

The invention discloses a new kind of nerve allotransplant, the preparation method and its medical application. The invention employs chemical extraction method to remove the schwann cell, axon and myelin sheath that can cause rejection for nerve allotransplant, stores stroma cell and three-dimensional pipeline structure for axon regeneration, and provides intact regenerative frame. The physical and biochemical property of nerve allotransplant are similar to that of normal nerve, and can be used as nerve allotransplant for damaged nerve.

The invention provides highly-oriented silk fibroin based nanofiber which adopts silk fibroin as a base material, adopts a nerve growth factor as a functional material for inducing neuronal cell growth and is obtained by an in-phase spinning controller with special design through an electrospinning fiber technique. Compared with traditional nano preparation technologies such as freeze drying, gas foaming, self-assembling and the like, the nerve growth factor loaded highly-oriented silk fibroin nanofiber scaffold as an artificial nerve graft has the advantages of high efficiency, simple process, low cost and the like, has structural and functional controllability which is not likely to be realized with the traditional nanotechnology, and is better in forming effect. Meanwhile, a composite of a biological material and cells is directly spun into a composite of a bracket material and the cells once with a blended spinning technology, and compared with a tradition process of firstly bracket material preparation and later cell culture on the bracket material, the process is simple.

The invention belongs to the field of medical biological materials capable of being implanted in human bodies, and relates to tissue engineering nerve graft for repairing injured nerves and a method for preparing the tissue engineering nerve graft. The tissue engineering nerve graft and the method have the advantages that micro-pattern techniques and freeze-dry molding processes are combined with one another, accordingly, an artificial nerve conduit favorable for adhesion and growth of nerve cells can be provided, orientation stripe structures are arranged on the inner walls of the conduit, the walls of the conduit are of loose porous structures, and suture or adhesion of the side walls of the conduit can be omitted during usage; the method for preparing the nerve conduit is simple and feasible and is applicable to mass production.

The subject invention pertains to materials and methods for processing tissue to produce a natural, acellular replacement tissue that is immunocompatible with a recipient. According to the subject invention, harvested tissue is subjected to wash solutions wherein only amphoteric detergent(s) are used (e.g., anionic detergents are excluded). Following extraction by amphoteric detergent(s), the tissue is washed with a buffer system to facilitate the clearance of cellular components and detergent. In one embodiment, the subject invention pertains to a replacement tissue that is a nerve graft that supports axonal regeneration, guides axons toward the distal nerve end and / or is immunologically tolerated. Preferably, the nerve graft retains essential extracellular matrix scaffolding as well as biological components that promote nerve regeneration through the nerve graft.

A medical artificial nerve graft containing silk fibroin used in bridging of nerve damage and its preparation method. The medical artificial nerve graft comprised of vessel or comprised of vessel and fibre scaffold, wherein at least one of the vessel and the fibre scaffold contains component of silk fibroin, and the wall of the vessel has three-dimensional structure provided with numerous microaptures. The preparation method includes the following steps: silk is used to prepare silk fibre; the silk fibroin fibre is used to prepare the vessel or the fibre scaffold containing silk fibroin; and combining the fibre scaffold with the vessel i.e. inlaying the fibre scaffold into the vessel and so on.

The invention relates to an artificial nerve graft constructed based on a chip-type acellularized scaffold and a preparation method of the artificial nerve graft, belonging to the field of tissue engineering. According to the artificial nerve graft and the preparation method, heterogeneous animal medulla spinalis, skeletal muscle or tendon acellularized tissue slices are adopted as a bracket, genipin is adopted for crosslinking cytokines, autologous ecto mesenchymal stem cells are planted on the bracket, and fibrous protein is taken as an adhesive for constructing the artificial nerve graft, so that the repairing of the nervous tissue is promoted. The artificial nerve graft and the preparation method have the advantages that the preparation cost is low, the period is short, the preparation raw materials are easily available, and the preparation is simple and is easy to operate. The constructed artificial nerve graft has low immunogenicity, contains a large number of autologous stem cells and cell factors, and is beneficial for adjusting the nerve injury microenvironment, and promoting the nerve regeneration and myelinogenesis. The artificial nerve graft can be used for preparing peripheral nerve and medulla spinalis defect, and has the broad application prospect.

The invention discloses a double-silk fibroin nerve graft. The double-silk fibroin nerve graft is characterized by comprising a skeleton and a tussah silk fibroin shell, wherein the skeleton is produced by mulberry silk fibers through weaving. By combining the property characteristics of mulberry silks and tussah silks, the mechanical properties of a silk fibroin nerve graft are improved. The prepared double-silk fibroin nerve graft has high mechanical strength and good flexibility and can resist the stretching of a suture line, and when the formation of a conduit is 50%, the compressive strength can reach 1.8N; and the double-silk fibroin nerve graft has very important clinical significances to the rapid acceleration of nerve regeneration and the long-distance restoration of defected nerves.

The invention discloses a tissue engineering nerve graft with a suspension fiber scaffold. The nerve graft comprises a conduit and tube fiber scaffolds. The nerve graft is characterized in that a plurality of suspensions are arranged on each fiber scaffold along the fiber length direction, and are connected with the inner wall of the conduit; and the fiber scaffolds are distributed and suspended in the conduit by virtue of the suspensions. By utilizing bio-printing technology, built-in microfilaments of a tissue engineering nerve graft can be stably and uniformly distributed in the conduit by utilizing the supporting force of the suspensions, and each fiber scaffold has an independent space to guarantee that nerve cells can directionally grow along the fiber scaffolds and cannot have error bridging, so that the success rate of operations can be greatly improved.

The invention discloses a chitosan artificial nerve graft containing a neurotrophic factor and a preparation method thereof. The chitosan artificial nerve graft is prepared by processing chitosan into a nerve conduit with a porous structure and high tensile strength by virtue of weak acid dissolution, mold injection, freezing formation, neutralization and immobilization, cleaning, lyophilization and other processes under a condition of not adding any foaming agent or cross linking agent. The cell factor with a treatment effect in the nerve conduit is one or more of a basic fibroblast growth factor (bFGF), a brain derived neurotrophic factor (BDNF), a neurotrophic factor 3 (NT-3), a basic fibroblast cell growth factor (bFGF) and a glial-cell-line derived neurotrophic factor (GDNF) and the like. The product provided by the invention contains the neurotrophic factor, adopts degradable materials, and has excellent biocompatibility with a human body. The prepared product does not contain exogenous toxic or side-effect substances brought by a preparation process.

The invention discloses a nerve conduit capable of improving the axonal regeneration orderliness and a preparation method thereof, and relates to the field of nerve conduits. The nerve conduit capableof improving the axonal regeneration orderliness disclosed by the invention comprises a conduit body and axonal inhibition molecules which are arranged in the conduit body and has the inhibition effect on the axonal regeneration; a nerve regeneration channel extending along the length direction of the conduit body is arranged in the conduit body; the axonal inhibition molecules are uniformly distributed in a conduit body substrate material and the inner wall of the nerve regeneration channel. Through the nerve conduit, the condition of axonal disorderly growth in nerve grafts can be reduced;the mismatch rate of axonal and target organ is favorably reduced.

The subject invention pertains to materials and methods for processing tissue to produce a natural, acellular replacement tissue that is immunocompatible with a recipient. According to the subject invention, harvested tissue is subjected to wash solutions wherein only amphoteric detergent(s) are used (e.g., anionic detergents are excluded). Following extraction by amphoteric detergent(s), the tissue is washed with a buffer system to facilitate the clearance of cellular components and detergent. In one embodiment, the subject invention pertains to a replacement tissue that is a nerve graft that supports axonal regeneration, guides axons toward the distal nerve end and / or is immunologically tolerated. Preferably, the nerve graft retains essential extracellular matrix scaffolding as well as biological components that promote nerve regeneration through the nerve graft.

The invention discloses a heterologous decellularized nerve graft with a tissue adhesion preventing function and a preparation method thereof. The heterologous decellularized nerve graft capable of preventing tissue adhesion consists of a heterologous decellularized nerve graft and an anti-adhesion macromolecular layer attached to the surface of the heterologous decellularized nerve graft; the heterologous decellularized nerve graft is an extracellular matrix which is obtained through subjecting in-vitro peripheral nerves of a healthy mammal to decellularizing. The anti-adhesion heterologous decellularized nerve graft disclosed by the invention is wide in source, low in price, good in biocompatibility and safe in use and meanwhile is free of immunogenicity; a microstructure of the extracellular matrix of the peripheral nerves is completely reserved, and thus, a natural piping support and a nutrition environment are provided for regenerating nerve axons and restoring a nerve conduction function; the anti-adhesion macromolecular layer of the heterologous decellularized nerve graft capable of preventing tissue adhesion, disclosed by the invention, can be changed into gel coats which are attached to surfaces of the decellularized nerves after the heterologous decellularized nerve graft is grafted in vivo, so that the action of preventing heterologous decellularized nerves from being adhered to periphery tissue can be excellently exerted.

The present invention discloses an artificial nerve graft prepared by electrostatic spinning, the preparing method and a special apparatus used therefor. Said artificial nerve graft is in the shape of a tube composed of nano-fiber that is prepared by electrostatic spinning of a polymer. The materials used in the present invention are bio-degradable materials and of desirable biocompatibility with human body. The product of the present invention is free of exogenous toxic substances or substances having side effects. Furthermore, the tube wall is of a 3-dimensional structure having micropores contained therein thereby providing a path for supplying nutritions required for the growth of nerve cells. Another advantage of the present invention is that necessary induction and space are provided for the growth of the nerve cells.

The present invention provides a conductive hydrogel comprising a polystyrene sulfonate compound selected from poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), reduced graphene oxide polystyrene sulfonate (rGO:PSS) and rGO:PEDOT:PSS; and a conjugate of polyethylene glycol (PEG) and a linker-(BX)n oligopeptide of formula (I)PEG-linker-(BX)n   (I)wherein B is lysine or arginine, X is selected from alanine, glycine, serine, threonine, tyrosine, glutamic acid or aspartic acid and n is an integer selected from 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and 20. The invention further relates to processes for assembling the conductive hydrogel. The conductive hydrogel can be used in various biomedical applications, such as neuroprostheses, biosensors, nerve grafts, cell culture and encapsulation of cells and microorganisms as well as for drug delivery.

A tissue engineered nerve graft for repairing peripheral nerve injury consists of a nerve conduit and a extracellular matrix (ECM) that is secreted by autologous or allogeneic support cells and obtained by decellularization. A preparation method of the ECM-modified tissue engineered nerve grafts containing support cells, nerve conduit and constructing a ECM-modified tissue engineered nerve graft.

The invention discloses an artificial nerve graft of a neurotrophic factor fixed by genipin and a preparation method thereof. A product comprises a nerve graft scaffold material, wherein the neurotrophic factor which is cross-linked by the genipin is arranged on the nerve graft scaffold material. The preparation method comprises the following steps of: adding a genipin solution and a neurotrophic factor solution into the solution of the nerve graft scaffold material, then molding and processing the solution, solidifying and cross-linking to obtain the required artificial nerve graft. The product contains the neurotrophic factor, and all the used materials are degradable materials which have favorable biologic compatibility with a human body. The prepared product does not contain external toxic or side effect substances carried in because of the preparation process.

The invention relates to a nerve graft conduit based on chitosan and a preparation method thereof. The nerve graft conduit based on chitosan is the conduit prepared by taking chitosan as a main raw material, the degradation time in a human body is accurately controlled to be 1 week to 6 months, the porosity of the conduit is 65-85%, the molecular weight distribution of the chitosan is 10K-500k, according to the porosity and the molecular weight of chitosan, the conduit can be divided into a short-term-use conduit, a middle-term-use conduit and a long-term-use conduit , the nerve conduit has good biocompatibility with the human body, the nerve conduit with corresponding repair time can be made according to nerves at different parts, different defect lengths and the thickness of defective nerves, the nerve graft conduit meets the nerve repairing growth rule, the use effect is good, and the nerve graft conduit can be widely applied clinically.

The invention provides a nerve graft and a nerve graft system using the same, and relates to the technical field of medical engineering. The nerve graft provided by the invention comprises a sensory nerve bundle passage, a moving nerve bundle passage and a mixed nerve bundle passage; all nerve bundle passages are matched with the nerve bundles of the corresponding sections of normal nerve, and are respectively filled with NGF (nerve growth factor), GDNF (glial cell-line derived neurotrophic factor), and NGF and GDNF. The nerve graft provided by the invention is matched with the nerve section to be restored in appearance; the inside nerve bundle passage trend is also precisely matched with the nerve bundle to be restored. Secondly, different nerve nutrition factors are respectively loaded in the nerve graft; the promotion and the direction guide can be performed for the nerve bundles with different functions; the matching rate of the nerve graft and the receptor nerve can be improved; the classified guidance on different nerve bundles can be realized; the nerve restoration effect is improved. In addition, the invention also provides the nerve graft system using the nerve graft so as to treat complicated nerve defect diseases and patients.

The invention discloses a nerve graft preparing method and a product of a nerve graft. The preparing method comprises the following steps of extracting pig nervous tissue, removing adipose tissue and connective tissue which are connected with the nervous tissue, freeze thawing through liquid nitrogen, chemically washing and sterilizing. According to the nerve graft preparing method, two methods of freeze thawing and chemical washing are combined. Thus, the structural integrity of a nerve tissue skeleton is improved, cellular components in the pig nervous tissue are completely removed, and the immunity is reduced. The nerve graft product prepared from the nerve graft can achieve goals of quick growth of nerves and functional recovery and provides a feasible program for clinical treatment.

The invention provides a melatonin nerve conduit composition and a nerve conduit, as well as a preparation method and application thereof. The composition comprises a biodegradable material, melatoninand a biocompatible adhesive material; various conduits applied to biomedicine are molded by utilizing 3D printing or co-melting of an electrostatic spinning biodegradable material and melatonin or an organic solvent; and after the conduits are molded, a biological adhesive substance layer is printed or coated to the inner wall according to requirements, and cells are induced to differentiate andgrow in conduits. The invention further provides a conduit bracket essential to biology, which is prepared from an ideal biomedicinal functional material, and has the advantages of simple preparation, low cost, easily controlled quality, wide application and the like. Sciatic nerve defect repairing experiments for rats indicate that the melatonin nerve conduit can be used for promoting nerve regeneration, improving maturation of regenerated nerves, increasing the number of effective nerve fibers and promoting functions of sciatic nerves to be restored to normal, has the effect superior to autologous nerve grafting restoration, and has a good application prospect.

Techniques are described for determining the quality of a nerve graft by assessing quantitative structural characteristics of the nerve graft. Aspects of the techniques include obtaining an image identifying laminin-containing tissue in the nerve graft; creating a transformed image using a transformation function of an image processing application on the image; using an analysis function of the image processing application, analyzing the transformed image to identify one or more structures in accordance with one or more recognition criteria; and determining one or more structural characteristics of the nerve graft derived from a measurement of the one or more structures.