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Nerve conduit capable of improving axonal regeneration orderliness and preparation method thereof

A nerve conduit and axon regeneration technology, applied in the field of nerve conduits, to achieve the effect of improving orderliness

Inactive Publication Date: 2018-06-22
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing techniques, whether by loading nerve regeneration-promoting substances in grafts such as nerve conduits and / or removing substances that inhibit axon growth, can promote axon growth and increase the number of axon regeneration, but the morphology Increases in the number of upper axons did not bring about a commensurate degree of functional recovery

Method used

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  • Nerve conduit capable of improving axonal regeneration orderliness and preparation method thereof
  • Nerve conduit capable of improving axonal regeneration orderliness and preparation method thereof
  • Nerve conduit capable of improving axonal regeneration orderliness and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Please refer to Figure 1-Figure 4 , the nerve guide 100 that can improve the orderliness of axon regeneration provided by this embodiment includes:

[0044]The tube body 101 made of gelatin and the axon-inhibiting molecules distributed in the tube body 101 that have an inhibitory effect on axon regeneration are chondroitin sulfate proteoglycans 102 (CSPGs) in this embodiment.

[0045] Wherein, the inner wall of the tubular body 101 has a plurality of nerve regeneration channels 103 extending along the length direction of the tubular body 101; The channels 103 are distributed in the inside of the tube body 101 in a manner of distributing along the length direction.

[0046] In addition, the nerve regeneration channel 103 is filled with hydrogel 104 that supports and promotes the growth of nerve fibers.

[0047] In addition, the outer diameter of the nerve guide 100 is about 2-5 mm; the diameter of the nerve regeneration channel 103 is about 100-300 μm. Of course, in ...

experiment example 1

[0060] Effect of CSPGs distribution on axon regeneration

[0061] Methods: (Adult SD rats were modeled with sciatic nerve clamp injury, and the SD rats were euthanized 7 days after the injury, and the injured sciatic nerve was taken, fixed in paraformaldehyde, and frozen sectioned, and the longitudinal field of view was taken, followed by immunofluorescence The staining method specifically marked axons (NF200) and chondroitin sulfate proteoglycans (CSPGs), and the results were as follows Figure 5 shown.

[0062] from Figure 5 It can be seen that the effect of axon regeneration after 7 days of injury repair is related to the distribution of CSPGs. Where the distribution of CSPGs is regular, the axon runs smoothly (arrow), while where the distribution of CSPGs is chaotic, the regeneration of axon is disordered (as indicated by the arrow in the figure).

experiment example 2

[0064] Flat CSPGs interface guides axonal steering

[0065] Method: Smear CSPGs on the bottom of the culture dish pretreated with poly-lysine to form the boundary of CSPGs, then plant DRG neurons in the culture dish, fix the cells with paraformaldehyde after a period of culture, and perform immunofluorescence staining Mark the axons, observe the growth of the axons at the boundary of CSPGs under a microscope, the results are as follows Image 6 shown.

[0066] Image 6 -B1 shows that DRG neurons grow and form distinct axonal boundaries in culture plates coated with CSPGs ( Image 6 -B1 indicated by the arrow); Image 6 -C shows that NF200 immunofluorescence staining shows that axons turn around near the CSPGs interface, and the enlarged images of rectangular boxes (corresponding to 1, 2, 3, 4) show the angle of axon turning; Image 6 -D shows that the proportion of axons that are turned (0.88±0.09) by the CSPGs interface is much higher than the proportion of axons that are...

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Abstract

The invention discloses a nerve conduit capable of improving the axonal regeneration orderliness and a preparation method thereof, and relates to the field of nerve conduits. The nerve conduit capableof improving the axonal regeneration orderliness disclosed by the invention comprises a conduit body and axonal inhibition molecules which are arranged in the conduit body and has the inhibition effect on the axonal regeneration; a nerve regeneration channel extending along the length direction of the conduit body is arranged in the conduit body; the axonal inhibition molecules are uniformly distributed in a conduit body substrate material and the inner wall of the nerve regeneration channel. Through the nerve conduit, the condition of axonal disorderly growth in nerve grafts can be reduced;the mismatch rate of axonal and target organ is favorably reduced.

Description

technical field [0001] The invention relates to the field of nerve guides, in particular to a nerve guide capable of improving the orderliness of axon regeneration and a preparation method thereof. Background technique [0002] The main obstacle restricting the repair of peripheral nerve defects is the lack of suitable repair materials. The use of tissue engineering principles to construct biomimetic peripheral nerve grafts is a research hotspot today, of which structural bionics and internal environment bionics are two major topics in this field. [0003] Nerve sleeves, nerve conduits, and decellularized nerve grafts made of biocompatible materials mimic the scaffolding structure of natural nerves to a certain extent, which can promote nerve regeneration to a certain extent, but are still far from ideal functional restoration. Many studies have confirmed that certain neurotrophic factors, extracellular matrix (ECM), drugs, and stem cells can promote peripheral nerve regener...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/58
CPCA61L27/54A61L27/58A61L2300/252A61L2300/40A61L2430/32
Inventor 朱庆棠邹剑龙邱帅孙佳慧全大萍刘小林陈诗浩李家春何富林
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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