The invention provides an orientated nano fiber bionic nerve conduit and a manufacturing method thereof. A nano fiber membrane with orientation degree larger than or equal to 75% is prepared by means of coaxial electrostatic spinning, and then is curved to form the nerve conduit, NGF (nerve growth factor) is added into fibers so that the bionic nerve conduit is combined with various factors promoting nerve regeneration, such as 'nano bionics', 'contact guiding', 'drug release' and the like, and the bionic nerve conduit can effective promote nerve regeneration as proved by animal experiments.