320results about How to "Good cell compatibility" patented technology

The invention discloses a gold nanotube based flexible stretchable electrode and a preparation method and application thereof. The preparation method comprises the following steps: performing hydrophilic treatment on a polydimethylsiloxane (PDMS) elastic film in advance and boosting uniform adhesion and fixing of a silver nanowire on the surface of an elastic base; with a disordered silver nanowire as a template, further preparing a gold nanotube with large length-diameter ratio through a replacement reaction of [Au(en)2]Cl3 and silver, thereby acquiring the gold nanotube based flexible stretchable electrode; connecting the electrode with an external electrode wire, thereby lastly acquiring an electrode used for testing. The gold nanotube based flexible stretchable electrode provided by the invention has the characteristics of high mechanical deformation resistance, excellent electrochemical property and excellent biocompatibility, can be used for monitoring mechanical sensitive cells in real time under true physiological conditions, and breaks through limitation to application of an existing nanometer material to a stretchable electrochemical transducer. The preparation method is simple and controllable and thus the electrode is easy to prepare.

The invention relates to a preparation method for a conduction and sustained release type nervous tissue engineering scaffold. The method comprises the following steps: silk fibroin Silk and lactic acid-caprolactone copolymer are dissolved in a solvent, dissolved and stirred to obtain a solution, then polyaniline and camphorsulfonic acid are added and stirred and mixed uniformly to obtain a cortex electrospinning solution, and NGF (nerve growth factors) are dissolved in ultrapure water completely to obtain a core electrospinning solution; and the cortex electrospinning solution and the core electrospinning solution are accommodated in an injector respectively for coaxial electrospinning, and then fumigation treatment and vacuum drying are performed to obtain the conduction type nervous tissue engineering scaffold. According to the prepared nanofiber scaffold, the nerve repair speed is increased from ways including external electrical stimulation (conductive polymer), biochemistry (NGFs), a topological structure required by nerve regeneration (orientation guide) and the like. The method is simple to operate, good in repeatability and high in economic benefit, and provides novel experimental thought for nerve defect repair in clinical application.

The invention relates the method for preparing rich silver antibiotic film used for medical pyrolytic carbon and TiN film. It solves the antibiosis question. The method comprises the following steps: on the pyrolytic carbon: a buffing and cleaning; b evacuating; c sputtering and cleaning for 5 minutes with 1keV nitrogen ion; d injecting silver ion: 70, 30keV energy, 2-8mA beam current, 2X1014/cm2-3X1014/cm2 dosage, and forming rich silver antibiotic film and antibiosis ratio being 100%; on TiN film: a buffing and cleaning; b evacuating; c sputtering and cleaning for 5 minutes with 1keV nitrogen ion; d sputtering Ti target with 3keV, 80mA Ar+ ion, 20-40keV, beam current: bombarding with 2-8mA high-energy N+ ion for 10-20 minutes; 50-350eV, beam current: bombarding with 10-30mA low-energy N+ ion for 60-180 minutes to deposit, N2 air pressure being 8X10 3Pa; e injecting silver ion: 30-80keV, beam current: 2-8mA, 1X1017/cm2-5X1018/cm2 dosage, forming rich silver antibiotic film and antibiosis ratio being 90%. The film has the advantages of good abradability, strong adhesive force and anti-corrosion, good cell compatibility and long antibiosis time-effect. The film can be used to prepare artificial heart valve and hard tissue alternate material which is implanted in human.

The present invention relates to a bone substituted material. Said bone substitute material is dicalcium silicate coating layer deposited on the surface of titanium alloy of Ti-6Al-4V, said coating layer is formed from beta-Ca2SiO4 and glass phase, and its thickness if 50-400 micrometers. Its preparation method incldues the following steps: firstly, synthesizing gamma-Ca2SiO4, then using atmospheric plasma spraying process to deposit the dicalcium silicate coating layer on the titanium alloy base body, and the combination strength of said coating layer and base body is greater than 34-40 MPa.The tests show that the bone apatite can be formed on the surface of dicalcium silicate, and cell can quickly grow and proliferate on the beta-Ca2SiO4 coating layer surface, so that said substitute material possesses good biological activity and compatibility.

The invention discloses an artificial blood vessel stent with aligned fibers and a manufacturing method of the artificial blood vessel stent, and belongs to the field of tissue engineering. According to the artificial blood vessel stent provided by the invention, a bio-compatible polymer material is taken as a spinning solution; firstly, the influence of different rotating speeds on fiber alignment is researched by virtue of an electrostatic spinning method, so that a rotating speed that the best alignment degree is achieved is determined; and then, the aligned fiber artificial blood vessel stent is manufactured on the basis of the rotating speed; the artificial blood vessel stent is relatively good in biocompatibility; and in addition, the alignment of smooth muscle cells on blood vessels can be guided. The artificial blood vessel stent provided by the invention is applicable to large-scale production and is expected to be applied to actual clinical diagnosis.

The invention provides an inflammatory microenvironment responsive smart drug-loaded hydrogel and a preparation method and application thereof. The preparation method for the inflammatory microenvironment responsive smart drug-loaded hydrogel comprises the following steps: a functional polymer containing ortho-hydroxyl reacts with phenylboronic acid containing amino or carboxyl to prepare a phenylboronic acid polymer; an amphiphilic drug carrier is prepared; the prepared amphiphilic drug carrier and a hydrophobic drug are dissolved with a good solvent, and then, water is added thereinto understirring to prepare a drug-loaded micellar solution; and the hydrophilic drug and the prepared phenylboronic acid polymer are dissolved in the prepared drug-loaded micellar solution, and then, the pHvalue of a mixed solution is regulated to 8-9 to prepare the smart drug-loaded hydrogel. The hydrogel responsively releases drugs through pH and reactive oxygen, and has the advantages that the preparation components are few and the synthesis operation is simple.

The invention relates to a method for preparing a porous bone scaffold by laser and increasing mechanical and biological performances of the porous bone scaffold by adding zinc oxide with little amount, which belongs to the bone tissue engineering field. Aiming at uncontrollable performances of aperture distribution, shape, space direction and connectivity in the preparation method of a traditional bone scaffold, and aiming at the disadvantages of poor mechanical property and fast resolving rate existed in tricalcium phosphate (beta-TCP), the invention provides the method for preparing the porous bone scaffold by using a selective laser sintering (SLS) technology, and provides the method for increasing performance by adding zinc oxide (ZnO). The method for preparing the porous bone scaffold has the advantages that the porous scaffold which enables three-dimensional interconnection is prepared by SLS, zinc oxide with little amount is added for introducing into a liquid phase, the particles densification can be promoted, crystal grain is refined, and the mechanical performance is improved, simultaneously, cell compatibility is increased, the degradation rate is reduced, and the biological performance is increased, so that the interconnected porous beta-TCP bone scaffold with enhanced mechanical performance and good biological performance can be finally prepared. The method has the advantages of simple preparation technology, convenient operation, low cost, easy control of technical parameter and the like.

The invention belongs to the field of medical materials, particularly relates to N,O-carboxymethyl chitosan-polyaldehyde hyaluronic acid gel and use thereof, and aims at providing an anti-adhesion blocking agent which is good in anti-adhesion effect, good in biocompatibility and bioabsorbable. The N,O-carboxymethyl chitosan-aldehyde hyaluronic acid gel provided by the invention has structural unit shown in a formula I described in the specification, wherein x=10-10,000 and y=10-10,000. The invention also provides a preparation method of the N,O-carboxymethyl chitosan-aldehyde hyaluronic acid gel, and use thereof in prevention and treatment of postoperative tissue adhesion. The prepared N,O-carboxymethyl chitosan-aldehyde hyaluronic acid gel provides a novel choice for the anti-adhesion blocking agent.

The invention discloses a biological small-diameter artificial blood vessel and preparation method thereof. The method comprises a step of crosslinking the natural extracellular matrix of vessels by using procyanidins. The small-diameter artificial blood vessel of the invention has the characteristics of improved mechanical strength and stability, non-toxicity, enzymatic degradation resistance in vivo, anti-calcification, platelet adhesion resistance, low immunogenicity, and very little protein release from cross-linking agent and the small-diameter vessel. These crosslinking characteristics are beneficial for the immigration, prolongation, and long-term anti-calcification of the small-diameter vascular smooth muscle cells and vascular endothelial cells of a human body itself after the small-diameter artificial blood vessel is implanted in the human body.

The invention relates to a preparation method of composite hydrogel with an interpenetrating network structure. The preparation method comprises the following process steps of: (1) preparing a collagen solution with acetic acid, and preparing methacrylated chondroitin sulfate solution and methacrylated hyaluronic acid solution with deionized water, distilled water or phosphate buffer solution; (2) adjusting the pH value of the collagen solution prepared in the step (1) to 7.0-7.4 with sodium hydroxide solution under stirring at normal pressure and 0-4 DEG C, then adding the methacrylated chondroitin sulfate solution and methacrylated hyaluronic acid solution prepared in the step (1) to the collagen solution under stirring, uniformly mixing three solutions, then adding phosphate buffer solution for diluting until the collagen concentration in the mixed solution is 5-9mg/mL, then adding an initiator and a catalyst, and uniformly mixing to form a precursor solution; and (3) injecting theprecursor solution prepared in the step (2) to a mold, and placing the mold holding the precursor solution in an environment of normal pressure and 37 DEG C for 30-60minutes.

The invention discloses a bioactive ceramic fiber composite scaffold for bone repair and a preparation method of the composite scaffold, and relates to the field of bone repair materials. In order toovercome the shortcomings of large brittleness, difficulty in processing, unsatisfactory degradability and biological activity and the like of a traditional ceramic scaffold material, bioactive ceramics such as calcium silicate serve as a scaffold body, and the surfaces of ceramic fibers are uniformly coated with a layer of degradable biomedical high polymer materials by an impregnation-centrifugation-drying process to obtain a high-porosity, good-mechanical-property and biodegradable bioactive ceramic fiber composite scaffold material for bone repair. The bioactive ceramic materials are easily subjected to synostosis with host bones, inorganic ions released by degrading the bioactive ceramic materials can participate in and promote osteogenesis and even angiogenesis metabolism activity ofa body and have irritating or inducing functions for tissue regeneration repair, and defective bone tissue repair and functional reconstruction are promoted. Degradation products of the biomedical high polymer materials on the surface of the scaffold are harmless and can be excreted to the outside of the body by metabolism.

The invention provides an enzyme-responsive intelligent bacterial adhesion resistant and bactericidal layer-by-layer self-assembled multi-layer film coating and a preparation method thereof. A surface-hydrophobic gel coating which is stable in a water environment is obtained through physical coating of chitosan, hyaluronic acid and a lysine solution, so that a material is endowed with bacterial adhesion resisting and contact sterilizing functions; polylysine improves the bacterial adhesion resistance of the material, so that the coating has the favorable gram-positive and gram-negative bacterial adhesion resisting and sterilizing capabilities. The preparation method is simple in process, quick, mild in condition, and easy for dip-coating, spin-coating, spray-coating and layer-by-layer self-assembly, can be implemented industrially, and has a wide application range, and the antibacterial performance, the bio-compatibility and the lubricity of the surface of medical devices can be effectively improved.

The invention relates to an organic-inorganic composite gel material for bone repair and a preparation method thereof. The composite gel material is characterized in that the organic material is formed by synthetic polymers or natural polymers; the inorganic material is formed by SiO2, CaO and P2O5 with mole fraction of 50-80%, 10-40% and 1-10% respectively; and the mass ratio of the organic material to the inorganic material is (0.1-1):1. The method is characterized by penetrating the polymer chains with biocompatibility and biodegradability into the SiO2 inorganic network structure to form the semi-interpenetrating structure. In the invention, the good blending of the organic and inorganic components at molecular level is realized, the mechanical property of the materials is simultaneously improved, and the bioactivity of the materials is improved; and the material and preparation method have important development potential in the field of bone defect repair.

The invention discloses a preparation method of chitosan/sodium alginate dual-network hydrogel, comprising the following steps of: carrying out a Schiff base reaction on a prepolymer solution composedof modified chitosan, modified sodium alginate, a photoinitiator and water to obtain single-network hydrogel; and reacting the single-network hydrogel under the irradiation of ultraviolet light to obtain secondary photo-crosslinked chitosan/sodium alginate double-network hydrogel, wherein the modified chitosan is carboxymethyl chitosan grafted with a collagen peptide; the modified sodium alginateis formylated sodium alginate modified by methacrylic acid. The collagen peptide disclosed by the invention has non-immunogenicity, and can be grafted on chitosan through an enzymatic reaction underthe condition of not using a toxic cross-linking agent, so that the cell compatibility and the cell proliferation promoting capability of the material are improved.

The invention discloses a large-aperture bone regeneration bracket material and a preparation method thereof, and belongs to the technical field of medical biomimetic material engineering. A natural porous inorganic biological material (cuttlebone) and ammonium dihydrogen phosphate undergo a full reaction at high temperature under high pressure so as to be transformed into a multi-phase biological ceramic (consisting of calcium carbonate, hydroxyapatite and calcium phosphate). The preparation method for the material is simple in process, the material source is abundant, and the cost is low. In addition, the prepared material has good biological compatibility and high compression strength, and is environmentally-friendly. The bone cell experiment shows that the material has bone conduction activity, can promote and induce bone regeneration self-repair, can be degraded step by step in vivo, is an ideal bone transplant replacement material, and has wide application range and obvious social and economic benefits in bone surgical operation.

The invention discloses a TiN-Ag nano composite coating and a preparation and application thereof. The TiN-Ag nano composite coating is characterized by being a composite film obtained by magnetron co-sputtering deposition; and a certain substrate temperature is kept in the sputtering process, and the obtained thickness is between 0.5[mu] m and 1[mu] m. The negative bias voltage of a substrate in the preparation process is adjusted by experiments; for an obtained TiN-Ag nano composite coating, the hardness of the substrate is remarkably improved; Ag elements are uniformly distributed in a TiN film, so a contact angle of the TiN film is significantly smaller than that of a TiN film in which no Ag is doped, good hydrophilicity is obtained and cell compatibility is improved; and the TiN-Ag nano composite coating is expected to improve application of a titanium alloy in biomedicine. The material formed by the method disclosed by the invention is stable in structure, the method can be repeated, and preparation conditions are controllable.

The invention discloses photocuring enzyme cross-linking bioink, a preparation method of the bioink and a 3D (three dimensional) printing method. The photocuring enzyme cross-linking bioink comprisesthe following ingredients: 0.1-50wt% of fibrinogen, 0.1-50wt% of methacrylic anhydride modified gelatin and 0.01-20wt% of photoinitiator. The photocuring enzyme cross-linking bioink has diversified material components; enzyme cross-linking of the fibrinogen is added based on ultraviolet light cross-linking of methacrylic anhydride modified gelatin; and a double-network hydrogel system comprising bioactive cells is constructed. A bionic structure printed by the photocuring enzyme cross-linking bioink has the characteristics of high cell compatibility, high mechanical strength, high degradability and no cytotoxicity, provides a good bionic environment for proliferation, differentiation and protraction of the bioactive cells and is applicable to the field of tissue engineering such as biological tissue construction and repair.