The invention discloses an autologous whole blood homogenate compound for reconstruction of an autologous skin epidermis protective screen. After the autologous whole blood is homogenized, under the synergetic effects of multiple bioactive substances and cell growth factors released by the autologous whole blood, the autoblood is effectively applied to epidermis repair and reconstruction of the skin protective screen, thereby giving better play to the maximum value and biological effects of the autoblood in autologous application; and because the autologous whole blood homogenate liquid plays a mutually cooperative protective effect with other auxiliary components and strengthens the compounding effect of the cell factors in epidermis reconstruction, the autologous whole blood homogenate liquid compound has good effects on epidermis repair and functional reconstruction of the epidermis protective screen, can improve the functional problems of the human skin protective screen in multiple aspects at different levels, restores the health of skin, and basically solves the functional problems of the epidermis protective screen. In addition, the preparation method disclosed by the invention is simple, convenient to operate, easy to learn and popularize, and suitable for medical cosmetology institutions with legal qualification for medical cosmetology.