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Growth-factor-containing nanofibre porous composite material capable of repairing bone and preparation method thereof

A technology for repairing materials and growth factors, applied in the field of biomedical materials, can solve problems such as the inability to fully simulate the physiological and microenvironment of bone tissue, and achieve osteoinductive effects, satisfying bone defect repair, and promoting bone tissue regeneration and functional reconstruction. Effect

Inactive Publication Date: 2011-08-03
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing bone growth factor sustained-release system cannot fully simulate the physiological microenvironment required for bone tissue regeneration.

Method used

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  • Growth-factor-containing nanofibre porous composite material capable of repairing bone and preparation method thereof
  • Growth-factor-containing nanofibre porous composite material capable of repairing bone and preparation method thereof
  • Growth-factor-containing nanofibre porous composite material capable of repairing bone and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Chitosan nanofiber composite biotype porous bone repair material containing BMP-2

[0039](1) Take by weighing 1.0g chitosan (deacetylation degree 75%, relative molecular mass 200000) and be dissolved in the acetic acid solution of 0.05% (v / v), be made into the solution that concentration is 0.01 (g / mL), then in Under stirring, add BMP-2 according to the mass ratio of BMP-2 and chitosan in the ratio of 0.1:1, and mix evenly;

[0040] (2) Weigh 0.5 g of biological porous bone repair material and immerse it in the solution prepared in step (1) under negative pressure, keep it for 1 hour, and take it out;

[0041] (3) freezing the porous bone repair material treated in step (2) at -80°C, and then freeze-drying for 24 hours;

[0042] (4) Immerse the porous bone repair material treated in step (3) in ethanol aqueous solution containing 0.1mol / L sodium hydroxide as a stabilizer (the volume ratio of ethanol to water is 80:20) for stabilization, and wash with dei...

Embodiment 2

[0052] Embodiment 2: Chitosan nanofiber composite TCP porous bone repair material containing bFGF

[0053] (1) Take by weighing 1.0g chitosan (deacetylation degree 100%, relative molecular mass 20000) is dissolved in the acetic acid solution of 0.025% (v / v), is made into the solution that concentration is 0.05 (g / mL), then in Under stirring, add bFGF at a ratio of 0.001:1 by mass ratio of bFGF to chitosan, and mix evenly;

[0054] (2) Weigh 0.5 g of calcium-phosphorous bioceramic porous bone repair material TCP and immerse it in the solution prepared in step (1) under negative pressure, keep it for 2 hours, and take it out;

[0055] (3) freezing the porous bone repair material treated in step (2) at -80°C, and then freeze-drying for 12 hours;

[0056] (4) Immerse the porous bone repair material processed in step (3) in ethanol aqueous solution containing 0.05mol / L sodium polyphosphate as a stabilizer (volume ratio of ethanol to water is 90:10) to stabilize, deionized water A...

Embodiment 3

[0060] Example 3: Chitosan nanofiber composite biological porous bone repair material containing BMP-2 and bFGF

[0061] (1) Take by weighing 1.0g chitosan (deacetylation degree 91%, relative molecular mass 100000) and be dissolved in the acetic acid solution of 0.05% (v / v), make the solution that concentration is 0.025 (g / mL), then in Under stirring, add BMP-2 and bFGF according to the mass ratio of BMP-2, bFGF and chitosan at a ratio of 0.1:0.001:1, and mix evenly;

[0062] (2) Weigh 0.5 g of biological porous bone repair material and immerse it in the solution prepared in step (1) under negative pressure, keep it for 2 hours, and take it out;

[0063] (3) freezing the porous bone repair material treated in step (2) at -80°C, and then freeze-drying for 24 hours;

[0064] (4) Immerse the porous bone repair material treated in step (3) in ethanol aqueous solution containing 0.1mol / L sodium hydroxide as a stabilizer (the volume ratio of ethanol to water is 95:5) for stabilizat...

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Abstract

The invention discloses a growth-factor-containing nanofibre porous composite material capable of repairing a bone and a preparation method thereof. The preparation method comprises the following steps of: dissolving chitosan in acetic acid; adding bone growth factors into the solution, and uniformly mixing the solution; immersing a porous inorganic material capable of repairing the bone in the solution under a negative pressure for 1-2 hours, and taking out the material; and freezing the porous material capable of repairing the bone at a low temperature, lyophilizing the material, immersing the material in a stabilizer solution to stabilize the material, washing the material with water, lyophilizing the material again, and sterilizing the material to prepare the growth-factor-containing nanofibre porous composite material capable of repairing the bone. In the invention, the growth-factor-containing nanofibre porous composite material capable of repairing the bone simulates the nanofibre structure of an extracellular matrix of the bone; the bioactivity of the bone growth factors can be kept, and the bone growth factors can be released continuously and slowly; the growth-factor-containing nanofibre porous composite material is beneficial for the regeneration and functional reconstruction of bone tissues; and the defect that traditional slow-release systems of the bone growth factors cannot better simulate a physiological microenvironment required for the regeneration of the bone tissues is overcome.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a bone repair material, in particular to a nanofiber composite porous bone repair material containing bone growth factor and its preparation method and application. Background technique [0002] Hard tissue diseases such as bone injury and bone defect caused by trauma, infection, tumor and abnormal development are frequently-occurring and common diseases worldwide. The clinical treatment methods for bone repair include autologous bone transplantation, allogeneic bone transplantation, artificial bone substitute transplantation and other methods. Autologous bone transplantation has the limitations of donor site damage, insufficient bone graft volume, and inability to prepare special shapes. Allogeneic bone grafts are prone to rejection. Therefore, artificial bone repair materials have become the main materials for clinical bone defect repair in the world. . [0003] At present...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/42A61L27/02A61L27/08A61L27/10
Inventor 曾戎汪炬王丁丁屠美赵剑豪孔杰
Owner JINAN UNIVERSITY
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