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Preparation method of dianthus chinensis magnolol

A technology of magnolol and dianthus, which is applied in the field of separation and preparation of dianthus magnolol, can solve the problems such as the preparation method of high-content dianthus magnolol, and achieve the effects of short preparation cycle, serious pollution and high yield

Inactive Publication Date: 2013-11-27
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no patent report on the preparation method of high-content caryophyllol in China

Method used

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  • Preparation method of dianthus chinensis magnolol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Get the stem bark of Magnolia officinalis, after crushing, weigh 1kg, add 5 times of 80% ethanol solution, the extraction voltage is 140V, the extraction time is 90s, carry out flash extraction, filter to obtain the extract, the extract is passed through a molecular weight cut-off of 3000 ultrafiltration membrane ultrafiltration and nanofiltration membrane concentration with a molecular weight cut-off of 500, the operating pressure is 0.8MPa, the membrane concentrate is concentrated and dried under reduced pressure to obtain a crude extract; high-speed countercurrent chromatography is used to separate and purify Dianthus in the crude extract Magnolol, with a volume ratio of 5:4:6:2 petroleum ether-ethyl acetate-methanol-water as a two-phase solvent system, the upper phase is filled with a high-speed countercurrent chromatographic column as the stationary phase, the host is rotated, and pumped into the lower phase to make The mobile phase, the crude extract dissolved in t...

Embodiment 2

[0023] Get the stem bark of Magnolia officinalis, after pulverization, weigh 1kg, add 12 times of 80% ethanol solution, the extraction voltage is 140V, the extraction time is 80s, carry out flash extraction, filter to obtain the extract, the extract is passed through a molecular weight cut-off of 1000 ultrafiltration membrane ultrafiltration and nanofiltration membrane concentration with a molecular weight cut-off of 500, the operating pressure is 1.8MPa, the membrane concentrate is concentrated and dried under reduced pressure to obtain a crude extract; high-speed countercurrent chromatography is used to separate and purify Dianthus in the crude extract Magnolol, with a volume ratio of 7:3:4:3 petroleum ether-ethyl acetate-methanol-water as a two-phase solvent system, the upper phase is filled with a high-speed countercurrent chromatographic column as the stationary phase, the host is rotated, and pumped into the lower phase to make The mobile phase, the crude extract dissolve...

Embodiment 3

[0025] Get the stem bark of Magnolia officinalis, after pulverization, weigh 2kg, add 10 times of 80% ethanol solution, the extraction voltage is 140V, the extraction time is 90s, carry out flash extraction, filter to obtain the extract, the extract is passed through a molecular weight cut-off of 1000 ultrafiltration membrane ultrafiltration and nanofiltration membrane concentration with a molecular weight cut-off of 300, the operating pressure is 2.0MPa, the membrane concentrate is concentrated and dried under reduced pressure to obtain a crude extract; high-speed countercurrent chromatography is used to separate and purify Dianthus in the crude extract Magnolol, with a volume ratio of 6:4:6:3 petroleum ether-ethyl acetate-methanol-water as a two-phase solvent system, the upper phase is filled with a high-speed countercurrent chromatographic column as the stationary phase, the host is rotated, and pumped into the lower phase to make The mobile phase, the crude extract dissolve...

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Abstract

The invention relates to a preparation method of dianthus chinensis magnolol, and the preparation method is easy to operate and less in pollution. The preparation method comprises the following process steps of: (1) taking the bark of magnolia obovata, carrying out crushing treatment, carrying out flash extraction by using an alcohol solution, and filtering to obtain an extracting solution; (2) treating the extracting solution through a membrane separation system, and concentrating and drying to obtain a crude extract; (3) separating and purifying the dianthus chinensis magnolol contained in the crude extract by adopting a high-speed counter-current chromatography, collecting dianthus chinensis magnolol components, wherein a two-phase solvent system is petroleum ether-ethyl acetate-methanol-water, an upper phase is a fixed phase, and a lower phase is a mobile phase; (4) carrying out vacuum concentration on the dianthus chinensis magnolol components, separating out crystals, and filtering the crystals to obtain the dianthus chinensis magnolol. The method disclosed by the invention is used for preparing the dianthus chinensis magnolol and high in product purity and easily realizes the industrialized amplification.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of traditional Chinese medicines, and relates to a method for separating and preparing dianthus magnolol from the bark of Japanese magnolia officinalis. Background technique [0002] Dianthus magnolol (Caryolane magnolol), is a kind of lignan compound, molecular formula: C 33 h 42 o 3 , molecular weight: 486.7, CAS accession number: 133056-11-8, structural formula: [0003] [0004] Dianthus magnolol is derived from the stem bark of Magnolia obovata Thunb. in the family Magnoliaceae. Dianthus magnolol has neurotropic effect in the neuronal cell culture system of rat fetal brain hemisphere; it can increase the activity of choline acetyltransferase. [0005] At present, there are no patent reports related to the preparation method of high-content dianthus magnolol in China. Contents of the invention [0006] The object of the present invention is to provide a kind of prep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C41/36C07C41/34C07C43/253
Inventor 刘东锋万冬梅
Owner NANJING ZELANG MEDICAL TECH
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