32 results about "Magnoliaceae" patented technology

The invention provides a method for grafting Michelia figo on magnolia denudate. The method includes the steps of scion and rootstock selection, grafting preparation, grafting and management after the grafting. Due to the facts that the magnolia denudata is provided with fleshy roots and root systems are strong, the survival rate of grafting seedlings is guaranteed. The Michelia figo and the magnolia denudata both belong to magnoliaceae, the scion and rootstock affinity is strong, the grafting success rate is improved greatly and the growth of the grafting seedlings after the grafting is also accelerated. The magnolia denudata has stronger cold-resistant capability and can safely live through the winter under the condition of minus 20 DEG C, so the grafted Michelia figo can be planted at cold northern regions and can have the capability of living through the winter in the open field. The method for grafting the Michelia figo on the magnolia denudate is simple, convenient and high in survival rate, greatly enhances the cold-resistant capability of the Michelia figo, achieves planting of the Michelia figo at the northern and northeast regions of China, and enables the Michelia figo to successfully live through the winter in the open field.

The invention provides a container nursery method for magnolia. The container nursery method includes harvesting seeds, cleaning seeds, drying seeds in shade, breaking dormancy to accelerate germination of the seeds, preparing base materials and containers, bagging the seeds, supplying nutrition to the seeds in black bags, cultivating by changing the black bags, and thereby obtaining the magnolia seedlings. Compared with the prior art, the container nursery method for the magnolia has the advantages that seedling cultivation time is shortened, the seeds begin to sprout after 20 days since the seeds are harvested and cleaned, the cultured seedlings are high in uniformity, the average sprouting percentage of the seeds is increased by 85% above, and the outplanting percentage is up to 95%.

The invention relates to the processing technics of Magnolia officinalis, comprising the following steps: a. the dried bark, velamen or branch bark of family Magnoliaceae plant Magnolia officinalis Rehd.Et Wils. or Magnolia officinalis Rehd.Et Wils.Var.biloba et.Wils. is taken and cleansed and the cork bark and impurity on fresh hide are removed; b. deactivation of enzymes; c. cutting; d. diaphoresis; the cut fresh Magnolia officinalis pieces are packed into a thick complex film package for diaphoresis 24-48 hours and are taken out after being completely discolored by diaphoresis; e. drying. The Magnolia officinalis pieces prepared for decoction prepared by the method contains active ingredient of high content, which is at least doubled compared with the content stipulated in the item of Magnolia officinalis of the pharmacopoeia of 2005 edition.

The invention discloses an acidic polyacrylamide electrophoresis separation method for peroxidase in Magnoliaceae plants, belonging to the technical field of electrophoresis methods. The acidic polyacrylamide electrophoresis separation method for peroxidase in Magnoliaceae plants comprises the following steps: pretreatment and extraction of peroxidase in the leaves of Magnoliaceae plants; unidirectional rapid separation of the peroxidase in the leaves of Magnoliaceae plants; and rapid color development via a benzidine method, and after completion of color development, rapid scanning of dyed gel by selecting a transmission cope mode in an EPSON scanner. According to the invention, acidic polyacrylamide electrophoresis is adopted for separation of the peroxidase in the Magnoliaceae plants, and overcomes the disadvantages of low resolution, small band numbers and the like in traditional polyacrylamide electrophoresis separation of peroxidase. The acidic polyacrylamide electrophoresis separation method of the invention has high application value in the identification, variety breeding and genetic relationship research of Magnoliaceae plants.

The invention is suitable for the field of magnoliaceae magnolia plant cultivation, and discloses a breeding method for tissue culture of axillary buds of magnolia sinostellata. The breeding method comprises the following steps: explant selection, explant treatment, anti-browning treatment, primary culture, subculture, rooting culture and seedling transplantation, wherein a culture medium for primary culture comprises an MS basic culture medium, 0.5-2 mg/L of 6-BA, 0.2-1 mg/L of KT, 0.05-0.2 mg/L of NAA, 0.05-0.1 mg/L of IBA, 7 g/L of agar and 30 g/L of cane sugar, and the pH value of the primary culture medium is 5.8-6.5; a culture medium for subculture comprises the MS basic culture medium, 0.5-1.5 mg/L of 6-BA, 0.05-0.2 mg/L of NAA, 0.05-0.1 mg/L of IBA, 7 g/L of agar and 30 g/L of canesugar, and the pH value of the subculture medium is 5.8-6.5; and a culture medium for rooting culture comprises a 1/2MS basic culture medium, 1-2 mg/L of NAA, 1-2 mg/L of IBA, 7 g/L of agar and 30 g/L of cane sugar, and the pH value of the rooting culture medium is 5.8-6.5. According to the breeding method for tissue culture of the axillary buds of the magnolia sinostellata, the propagation speedof the magnolia sinostellata can be increased, and the obtained magnolia sinostellata seedlings can maintain the characteristics of mother seeds.

The invention discloses an efficient evergreen magnolia plant defoliation method. The method comprises the following steps of thoroughly watering a to-be-treated plant once, and then carrying out drought stress under natural conditions; diluting a Guoguang ethephon water aqua with 40% of effective components by 100 times, and uniformly mixing the Guoguang ethephon water aqua with abscisic acid with the concentration of 30 mg/L according to an equal volume ratio of 1: 1; in a windless afternoon with sufficient illumination, carrying out pesticide atomized spraying treatment on the plant after drought stress to ensure that pesticide is sprayed to the front and back surfaces of all the leaves, wherein the treatment standard is that the pesticide on the leaf surfaces is in a water drop shape,the number of treatment times is 2, and the interval time is 1 day; and after plant induction is implemented, watering the roots thoroughly in time to wait for the leaves to fall off. The evergreen magnolia plant after artificial defoliation can be transported like deciduous magnolia with bare roots, the branches and the buds of the plant are not damaged, the plant is high in survival rate and fast in recovery after being replanted, and the problems of high circulation cost, difficulty in inspection and quarantine and the like can be effectively solved.

The invention discloses a manufacturing method of a magnoliaceae color-retaining specimen. The method can shorten the color retention time in the manufacturing process, so that the specimen is long in color retention and preservation time. The method comprises the following steps: (1) putting magnoliaceae into ethyl alcohol to be soaked for 20-40 min; (2) adding copper acetate powder into 50% glacial acetic acid until the solution is saturated, and diluting the solution to 3 times to obtain a color-retaining solution; (3) putting the magnoliaceae obtained in the step (1) into the color-retaining solution, performing heating and soaking until the color is greened again, and then soaking and cleaning the magnoliaceae with water; and (4) putting the magnoliaceaeon a water-absorbing towel to absorb surface moisture, putting the magnoliaceae into water-absorbing paper, pressing the magnoliaceae by using a specimen holder, drying the magnoliaceae by using a specimen dryer to obtain a magnoliaceae specimen, and performing vacuum film coating after the specimen is completely dried.

The invention discloses a method for controlling tall tree Icerya aegyptiaca. The method comprises the following steps: a container around a tree trunk is arranged at the bottom of the trunk of a tall tree, a water absorbing material is filled in the container to ensure that the water absorbing material contacts with the epidermis of the tree trunk, then diluted omethoate (the dilution multiple is 80-120) is injected in the water absorbing material until the water absorbing material is saturated, and the container is sealed. The method for controlling tall tree Icerya aegyptiaca is simple and effective for tall trees, particularly the crown part is effectively controlled; and the labor intensity is greatly reduced, and the risk that liquid medicine falls on the operator can be avoided. By adopting the method for controlling Icerya aegyptiaca, the effect is good, the normal growth and ornamental value of the tree can be ensured, and the popularization and application of tall trees of the Magnoliaceae and Moraceae in landscaping can be ensured.

The invention relates to a bio-based antibacterial adhesive, in particular, relates to a bio-based antibacterial adhesive and a preparation method thereof, and belongs to an adhesive synthesis technology. The bio-based antibacterial adhesive is a linear polymer which is obtained by taking 5',5-diallyl-2,2'-biphenol and 3,6-dioxa-1,8-octane dithiol as reactants, taking 2-isopropyl thioxanthone as a catalyst and taking methylbenzene as a solvent at room temperature under the irradiation of a 365 nm light source. The 5',5-diallyl-2,2'-biphenol disclosed by the invention is derived from a bio-based compound of a magnolia deciduous tree plant, and has good antibacterial property and biocompatibility. The bio-based antibacterial adhesive disclosed by the invention has the characteristics of biomass source, reproducibility, easiness in degradation, low energy consumption and simple process, and the economic value of the antibacterial adhesive is improved.

The invention relates to an ultraviolet fluorescent coating, in particular to a bio-based antibacterial ultraviolet fluorescent coating and a preparation method thereof, and belongs to a biomaterial synthesis technology. The bio-based antibacterial ultraviolet fluorescent coating is prepared by carrying out a cross-linking reaction on a prepolymer and a polythiol compound under the action of a catalyst 1, 1'-(methylenebis-4,1-phenylene)bis[2-hydroxy-2-methyl-1-acetone] (Irgacure127). Wherein the prepolymer is a linear polymer which is obtained by polymerizing 5', 5-diallyl-2, 2'-biphenol and 1, 6-hexanedithiol under the action of a catalyst azodiisobutyronitrile (AIBN). The 5', 5-diallyl-2, 2'-biphenol is derived from a bio-based compound of Magnoliaceae deciduous tree plants, and the bio-based ultraviolet fluorescent coating has the characteristics of being antibacterial, green and environment-friendly, renewable and biodegradable in raw materials, simple in process, wide in raw material source and the like; the risk of generating toxicity to a human body is avoided, and the application of the ultraviolet fluorescent coating is greatly widened.

The invention belongs to the technical field of electrophoresis, and discloses an acidic polyacrylamide electrophoresis separation method for Magnoliaceae plant seed prolamins. The acidic polyacrylamide electrophoresis separation method for Magnoliaceae plant seed prolamins comprises the following steps: extraction of prolamins in Magnoliaceae plant seeds, unidirectional rapid separation of the prolamins from the Magnoliaceae plant seeds, fixation, staining, and decolorizing. The method adopting acidic polyacrylamide electrophoresis to separate the Magnoliaceae plant seed prolamins is simple,is easy to perform, and provides a rapid and effective way for classifying and identifying seeds of different kinds of Magnoliaceae plants.

The invention discloses a hybrid liriodendron efficient transient gene expression method using suspension cell protoplast as the receptor. The method includes: preparing the hybrid liriodendron suspension cell protoplast, preparing plasmid, converting and observing. The method has the advantages that the hybrid liriodendron suspension cell protoplast is used as the receptor system for genetic transformation, a PEI mediated method is used, green fluorescent protein GFP genes are used as the reporter genes, and the efficient transient expression of the GFP genes is detected through a fluorescent microscope; the hybrid liriodendron suspension cells are used as the source of the protoplast, the hybrid liriodendron suspension cells are abundant, consistent in state, weak in background signal and convenient in material taking, and the built transient expression system can provide a fast and convenient operation platform for the researches of the gene functions of liriodendron and magnoliaceae plants.

The invention discloses 5- allyl-3'-(2,3-diallyl)-2,4'-biphenol novel compound (formula I) which is obtained by separating dry tree bark, root bark and wig bark from magnolia biloba (Magnolia officinalis Rehd. et Wils.var.biloba Rehd.et Wils ) of traditional Chinese medicine schisandra sphenanthera mangnolia officinalis (Magnolia officinalis Rehd. et Wils). According to the angiogenesis inhibiting activity research, the compound of the formula I has a good angiogenesis inhibiting effect. The 2,4'-biphenol compound can be used for preventing and treating the diseases including tumors, diabetic retinopathy, rheumatism, psoriasis, hemangioma and the like.

The invention belongs to the technical field of metal material corrosion protection and particularly relates to a corrosion inhibitor for calcium chloride antifreeze solution and a preparation method thereof. The corrosion inhibitor for calcium chloride antifreeze solution is prepared by adding trace additives into leaf extracting solution of magnoliaceae michelia deciduous tree michelia. The corrosion inhibitor has the advantages of being abundant in raw material source and low in cost and having good corrosion-inhibiting effects on various metal including steel, iron, copper, tin, aluminum and the like, and the preparation method has the advantages of being simple in process, convenient to operate, environment-friendly and convenient for industrial production and the like.