3203 results about "Cuticle" patented technology

This invention comprises administering to a human or animal in need of treatment an effective amount of an antioxidant lipoic acid derivative and / or pharmaceutically acceptable salts and solvates thereof for the treatment or prevention of pathological (inflammatory, proliferative and degenerative diseases, e.g. diabetes mellitus, atherosclerosis, Alzheimer's disease and chronic viral diseases) and non-pathological (e.g. skin aging and wrinkle formation) conditions caused by oxidative damage. Methods of synthesizing novel antioxidant lipoic acid derivatives and their use in preventing or treating diseases or conditions caused by oxidative stress and other free radical mediated conditions are described. Another aspect of this invention is the use of these antioxidant compositions for the protection of skin from damage caused by ultraviolet radiation and dessication, and to provide improved skin feel by desquamating, cleansing and clarifying the skin. The compositions described in this invention increase cellular viability of epidermal cells, promote cytoprotection, and decrease the production of inflammatory mediators such as inflammatory cytokines in these cells. The antioxidant compositions are incorporated into sunscreen products, soap, moisturizing lotions, skin toners, and other skin care products.

Systems and methods are provided for removing fatty tissue underlying a patient's epidermis (e.g., blepharoplasty, brow lifts, eyelid shortening procedures, and the like). These methods include positioning one or more active electrode(s) and one or more return electrode(s) in close proximity to a target site on an external body surface of the patient. A high frequency voltage difference is applied between the active and return electrode(s), and the active electrode(s) are translated across the external body surface to create an incision therein. The bipolar configuration controls the flow of current to within and around the distal end of the probe, which minimizes tissue necrosis and the conduction of current through unwanted paths in the patient. The residual heat from the electrical energy also provides simultaneous hemostasis of severed blood vessels, which increases visualization and improves recovery time for the patient.

This invention comprises administering to a human or animal in need of treatment an effective amount of an antioxidant lipoic acid derivative and / or pharmaceutically acceptable salts and solvates thereof for the treatment or prevention of pathological (inflammatory, proliferative and degenerative diseases, e.g. diabetes mellitus, atherosclerosis, Alzheimer's disease and chronic viral diseases) and non-pathological (e.g. skin aging and wrinkle formation) conditions caused by oxidative damage. Methods of synthesizing novel antioxidant lipoic acid derivatives and their use in preventing or treating diseases or conditions caused by oxidative stress and other free radical mediated conditions are described. Another aspect of this invention is the use of these antioxidant compositions for the protection of skin from damage caused by ultraviolet radiation and dessication, and to provide improved skin feel by desquamating, cleansing and clarifying the skin. The compositions described in this invention increase cellular viability of epidermal cells, promote cytoprotection, and decrease the production of inflammatory mediators such as inflammatory cytokines in these cells. The antioxidant compositions are incorporated into sunscreen products, soap, moisturizing lotions, skin toners, and other skin care products.

A treatment of sweat glands in a target region of skin includes generating electromagnetic radiation having a wavelength of about 1,064 nm to about 1,800 nm. To decrease sweat production in a plurality of sweat glands, the electromagnetic radiation is delivered to a dermal interface defined by a dermal region and a subcutaneous fat region in the target region of skin to cause thermal injury to at least one of the dermal region, the subcutaneous fat region or the dermal interface. An epidermal region of the skin can be cooled at least one of before, during or after delivering the electromagnetic radiation to the dermal interface in the target region of skin.

The present invention provides methods for diagnosing melanoma in a subject by analyzing nucleic acid molecules obtained from the subject. The present invention also provides methods for distinguishing melanoma from dysplastic nevi and / or normal pigmented skin. The methods include analyzing expression or mutations in epidermal samples, of one or more skin markers. The methods can include the use of a microarray to analyze gene or protein profiles from a sample.

Described is a process and compositions for counteracting human sweat malodor. The compositions containing at least 10 weight percent of either 3,7-dimethyl-2,6-nonadienenitrile or 3,7-dimethyl-2,6-octadienenitrile. Additional ingredients may be added including napthyl methyl ether, methyl beta-naphthyl ketone, benzyl acetone, methanoinden propionates, methyl ionone, tetramethylnaphtho furan, ethylene glycol cyclic ester of dodecanedioic acid, 1-cyclohexadecen-6-one, 1-cycliheptadecen- 10-one, and corn mint oil. These compositions are applied to either fabric or a defined surface area of the human epidermis.

The invention relates to a method and a process of determining individual skin structure and function at a point in time for the purpose of determining and formulating skin care products that remedy the deficiencies observed in the skin. Objective and repeatable dermal biometric instrumentation techniques can be used to measure skin moisture content, sebum content, firmness and elasticity properties, skin thickness, transepidermal water loss, skin pH and to perform a photo analysis of the face with UV and visible light. By customizing the skin care products, the individually added active ingredients can be controlled, the diluents can be modified, dermal penetration rates can be controlled, the surfactant systems can be adjusted, and the stability of the product can be controlled. To prevent the loss of active materials in the product, the skin care product is manufactured for an individual consumer and is only sold in a quantity of a three months supply. Additionally, a variety of ingredients can be combined that a mass produced product cannot contain due to stability / compatibility issues. This invention overcomes the limitations in formulating skin products for the mass market by providing a product designed with objective biometric data and created for the specific clinical condition of an individual's skin.

Two types of compositions having an eye-drop delivery system are used during or after an orthokeratology procedure to prevent or retard relaxation of corneal tissue back to the original anterior curvature of the cornea. Each composition functions independently from the others and is a different approach of preparing a stabilizing agent. The first composition is directed to a biologically compatible composition comprising fibril associated collagens with interrupted triple helices (FACITs) and / or small leucine-rich repeat proteoglycans (SLRPs). The fibril associated collagen family includes various types of collagens, such as type VI, type XX, type XII, and type XIV. The small leucine-rich repeat proteoglycans family includes decorin, keratocan, biglycan, epiphycan, lumican, mimecan, and fibromodulin. The second composition includes the enzyme found as a normal component of tissues, plasma, or epidermis, such as transglutaminase.

A deep acting topically working facial care appliance includes a dual action facial puck having a rotating and counter rotatable facial implement that is dually effective both to clean, massage, apply compositions and otherwise topically work the skin and to deeply act to penetrate below the epidermis and into the dermis massaging and deeply passivating and enervating the dermis and dermal collagen.

This invention relates to a method for amplifying neural stem cells in vitro by 3-dimensional culture. The method comprises: selecting microcarrier with 3-dimensional environment, pre-treating, coating the microcarrier with 40-60 ng / mL alkaline fibroblast growth factor, 40-60 ng / mL epidermal growth factor, and B27 DMEM / F12 neural stem cell serum-free culture medium, adding 1X105-1X106 neural stem cells into the culture bottle, taking out the microcarrier grown with neural stem cells, removing the microcarrier, and rinsing cells to obtain neural stem cells. The porous microcarrier can enlarge the culture area. The alkaline fibroblast growth factor and epidermal growth factor can promote cell multiple fission and improve cell microenvironment, which is advantageous for multiple fission of neural stem cells.

The invention relates generally to intradermal, transdermal, and / or transmembrane delivery of biologically active substances in the epidermis and / or through the skin, sub-dermal tissues, blood vessels and cellular membranes without causing damage to the cellular surface, tissue or membrane. The biologically active substances may have a molecular weight no less than about 5.8 kDa to about 2,500 kDa, such as Hyaluronic Acid (HA). The biologically active substances may be deposited in a dermal patch containing a red algae polysaccharide-based matrix, wherein the red algae polysaccharide is an extract of Chondrus crispus at 2% by weight of the dermal patch. The invention provides systems and methods for enhanced intradermal, transdermal, and / or transmembrane delivery of such biologically active substances using pulsed incoherent light. The invention further provides a device for the application of the pulsed incoherent light to cellular surfaces and membranes using those compositions and methods.

A biological tissue sheet which is expected as exerting a favorable therapeutic effect and a high safety in transplantation. The biological tissue sheet formed by (a) preparing in vivo-derived cells; (b) sowing the in vivo-derived cells on amniotic membrane; and (c) culturing and proliferating the in vivo-derived cells in the absence of any xenogeneic animal cells. As the cells of a biological origin, for example, cells originating in corneal epithelium, conjunctival epithelium, skin epidermis, hair follicle epithelium, oral mucosa, respiratory tract mucosa, or intestinal tract mucosa.

The present invention provides methods for characterizing a skin sample of a subject as belonging to an age range by analyzing nucleic acid or protein molecules obtained from the subject. The methods include analyzing expression or mutations in epidermal samples, of one or more skin markers. The methods can include the use of a microarray to analyze gene or protein profiles from a sample and compare them with a known skin age index. Therapeutic and cosmetic formulations are also provided herein.

This invention relates to a method and composition for enhancing epidermal and / or hair follicle stem cell production, cell renewal, and / or growth of the skin, hair and nails, by topical administration of a therapeutic dosage of cyanobacteria and green algae and / or both simultaneous enteral and topical administration of a therapeutic dosage of cyanobacteria and green algae.

The invention relates to a human mesenchymal stem cell culture medium. According to the culture medium, the basal culture medium comprises the following components based on the final concentration: 1-2g / L of human serum albumin, 5-10mg / L of transferring, 2-8mg / L of fibronectin, 1-4mg / L of laminin, 50g / L of Fe(NO3)3.9H2O, 417g / L of FeSO4.7H2O, 1-3mu g / L of estradiol, 2-5mu g / L of testosterone, 1-3mu g / L of progesterone, 39.25-117.74 mu g / L of dexamethasone, 5-10mg / L of insulin, 376.36mg / L of riboflavin, 80.96-242.87mg / L of coenzyme A, 4.41-6.17mg / L of butanediamine, 1-2mg / L of taurine, 0.61-1.85mg / L of aminoethanol, 8.81-26.42mg / L of pyruvic acid, 3.78-7.56mu g / L of sodium selenate, 292.3-584.6mg / L of L-glutamine, 2-8mu g / L of vascular endothelial growth factor, 4-10mu g / L of epidermal growth factor, 4-10mu g / L of basic fibroblast growth factor, 1-5mu g / L of leukaemia inhibitory factor, 1-5mu g / L of insulin-like growth factor-I and 2-8mu g / L of stem cell factor. The culture medium does not contain the animal serum, the potential animal endogenous endotoxin or virus of the animal serum is eliminated, and the culture medium is conveniently applied to clinics.

The invention discloses a silk fibroin micro-needle system, a silk fibroin nanometer particle and a preparation method thereof. The dissolvable silk fibroin micro-needle system comprises a base plate and a micro-needle, and is characterized in that a body of the micro-needle consists of silk fibroin protein condensate, the silk fibroin protein condensate penetrates into the skin of a user, and micro-pipes are remained on the skin of the user after the silk fibroin protein condensate is dissolved. The silk fibroin nanometer particle is characterized by comprising silk fibroin protein molecules and cosmetic molecules or medicine molecules, the silk fibroin protein molecules wrap the cosmetic molecules or the medicine molecules to form a particle structure, antibody or aptamer molecules can be attached onto the surface of the silk fibroin nanometer particle, and the degradation speed of the silk fibroin nanometer particle in the body of the user can be controlled by means of adjusting crystalline degree of silk fibroin protein. A method for delivering medicine or cosmetic is characterized by comprising steps of penetrating the silk fibroin protein condensate of the body of the micro-needle into the skin of the user, dissolving the silk fibroin protein condensate, and releasing nanometer silk fibroin particles wrapped with the medicine or the cosmetic so that the medicine molecules or the cosmetic molecules are delivered; or delivering the medicine molecules or the cosmetic molecules via the micro-pipes remained on the skin of the user after the silk fibroin protein condensate is dissolved in a permeation manner, and the like.

Provided is a method for obtaining human DNA for genetic analysis, by taking the epidermis of testee by means of an adhesive sheet, and by extracting DNA from the epidermis stuck on the adhesive sheet. Provided are also combined sheets for conveniently storing DNA and a kit for taking the epidermis and analyzing DNA. Along with the kits, the method allows DNA to be easily obtained and stably stored for a long period of time. In addition, both the identification and the DNA analysis of a testee can be conducted at the same time by taking epidermal scraps from the testee, along with a figured epidermal print.

The present invention is a method to manufacture shaped foam composite articles comprising a foam core and one or more skin and shaped foam composite articles made therefrom. Specifically, cold formed shaped foam articles having an upper and lower surface having a skin applied to one or both of the surfaces. Preferably the foam comprises a styrenic polymer foam and the skins may independently be mono-layered or multi-layered. The shaped foam article and the skin may be made from the same or different materials. In the case where there are more than one skin, the skins may comprise the same or different materials.

The invention provides a method for preparing beer by using pomelo. It comprises following steps: washing pomelo, removing epiderm (or not) and bad part, separating pulp from peel, cleaning peel, cutting peel, adding water and grinding, controlling temperature, adding proper amount of acid and stirring evenly, adding enzyme and getting peel enzyme reaction liquid, beating pulp, removing seed and getting pulp slurry, adding proper amount of acid and stirring, adding enzyme and getting pulp enzyme reaction liquid; mixing peel enzyme reaction liquid with pulp enzyme reaction liquid according to a certain proportion, stirring evenly and getting pulp slurry enzyme reaction liquid, filtering and getting pomelo clear juice; mixing filtered pomelo clear juice with brewed beer, loading, sealing, disinfecting, cooling, packing and getting pomelo beer; or mixing pomelo enzyme reaction liquid with prepared cereal mash, boiling, cooling, injecting yeast and preparing with beer processing steps and getting fermented pomelo beer.

The invention discloses a polymer micro-needle array chip. The chip comprises a micro-needle array and a substrate used for the standing of the micro-needle array; and the matrix material of the micro-needle array is a polyacrylamide polymer. The invention also discloses a preparation method of the polymer micro-needle array chip, a micro-needle transdermal drug delivery patch prepared through utilizing the polymer micro-needle array chip, and a preparation method of the patch. The micro-needle of the polymer micro-needle array chip has a high mechanical strength and a sharp needle point, so the skin cuticle can be easily pierced; the preparation method avoids a high-temperature processing treatment step, and is in favor of maintaining the activities of biomacromolecules comprising polypeptides, proteins and the like; the polyacrylamide polymer can easily dissolve or swell after meeting with the water-containing environment, and is in favor the sustained release of drugs in skins; and the preparation method of the polymer micro-needle array chip based transdermal drug delivery patch is simple, so the batch produced can be realized through utilizing present manure processing technologies.

The invention discloses a complete medium with low serum concentration for cultivating mesenchymal stem cells and a method for cultivating the mesenchymal stem cells using same. The complete medium comprises a cell basic medium, fetal calf serum with final concentration of 1-100 mul / ml, an epidermal growth factor with final concentration of 1-100 ng / ml, and a basic fibroblast growth factor with final concentration of 1-100 ng / ml. The complete medium with low serum concentration successfully reaches equal or even better function of promoting cell proliferation than a culture reagent with high serum concentration. The cultured cells have the typical biological characteristics of mesenchymal stem cells, and can also express an omnipotent mark of the embryonic stem cell and high express the idiosyncratic mark of the neuron under the condition of in vitro inducement. And the difference between the cell batches is little, the cost is low and the security is good. Compared with the prior cultivating method, the method has advantages of simple operation, low probability of pollution and high success ratio of cultivating cells.

The invention discloses an acne-removing scar-lightening mask solution which is prepared from the following components in percentage by weight: 0.01-1% of high-molecular humectant, 0.01-0.2% of disodium edetate, 0.01-0.5% of thickener, 5-25% of witch hazel extract, 0.01-2.0% of conditioner, 0.01-5% of desensitizer, 1-10% of low-molecular humectant, 1-8% of plant acne-removing agent, 0.1-0.5% of preservative, 0.01-0.5% of mung bean extract, 0.01-5% of epidermal growth factor and the balance of deionized water. The manufacturing method of the acne-removing scar-lightening mask solution is simple. Under the synergistic actions of the plant acne-removing agent and mung bean extract in combination with the epidermal growth factor and witch hazel extract, the mask solution has the quick effects of acne removal, itching relief and sterilization, and can restore the damaged skin and lighten the acne marks and scars while removing the acnes, thereby improving the skin from inside to outside and restoring the smooth, delicate and healthy skin.

A treatment for subcutaneous fat and / or cellulite includes delivering a beam of radiation to a subcutaneous fat region disposed relative to a dermal interface in a target region of skin. The beam of radiation affects at least one fat cell in the subcutaneous fat region without causing substantial unwanted injury to the epidermal region and causes thermal injury to a dermal region to induce collagen formation to strengthen the target region of skin in a target region of skin. The treatment can include cooling an epidermal region of the target region of skin.

The invention discloses a whitening composition and a skin care product with the same. The whitening composition and the skin care product contain glycyrrhiza glabra extracts, arbutin and 3-o-ethyl ascorbic acid, and preferably contain nonapeptide-1, nicotinamide, ethylhexyl methoxycinnamate or composition of the nonapeptide-1, the nicotinamide and the ethylhexyl methoxycinnamate. Through mechanism analysis, the composition has the advantages that possibly due to the mutual action and influence among various ingredients, the generation of melanin is inhibited through inhibiting the activity of tyrosinase; the migration of melanocytes to the skin is retarded; in addition, the plant and microbial peptide sourced whitening ingredients and various antiallergic ingredients are selected to be combined to obtain the safe and mild whitening effect.

The invention relates to cosmetic active ingredient-containing lipidosome as well as a preparation method and application thereof. The cosmetic active ingredient-containing lipidosome is small and uniform in particle size, so that the wrapped active ingredients can permeate the cuticles to the deep layer of skin so as to effectively realize deep skin care. Moreover, due to the small particle size, the collision and sedimentation probability of the finished lipidosome product is decreased and the stability of the lipidosome cosmetic is improved. The cosmetic active ingredient-containing lipidosome is prepared by a host-guest chemical method, so that the lipoid molecules and the active ingredient molecules are uniformly mixed on the molecular level, so that the gathering inactivation phenomenon of the active ingredients is avoided; the preparation condition is mild, so that the active ingredients are prevented from damage.