1711 results about "Biological macromolecule" patented technology

A process for preparing ultrafine powders of biological macromolecules comprises atomizing liquid solutions of the macromolecules, drying the droplets formed in the atomization step, and collecting the particles which result from drying. By properly controlling each of the atomization, drying, and collection steps, ultrafine dry powder compositions having characteristics particularly suitable for pulmonary delivery for therapeutic and other purposes may be prepared.

The present invention relates to a biologically active functionalized electrospun matrix to permit immobilization and long-term delivery of biologically active agents. In particular the invention relates to a functionalized polymer matrix comprising a matrix polymer, a compatibilizing polymer and a biomolecule or other small functioning molecule. In certain aspects the electrospun polymer fibers comprise at least one biologically active molecule functionalized with low molecular weight heparin. Examples of active molecules that may be used with the multicomponent polymer of the invention include, for example, a drug, a biopolymer, for example a growth factor, a protein, a peptide, a nucleotide, a polysaccharide, a biological macromolecule or the like. The invention is further directed to the formation of functionalized crosslinked matrices, such as hydrogels, that include at least one functionalized compatibilizing polymer capable of assembly.

Biological macromolecules are synthesized in vitro under conditions and in a reaction composition wherein oxidative phosphorylation is activated and protein folding is improved.

An apparatus for growing a biological macromolecular crystal by vaporizing biological macromolecular solution into an oversaturated state. The apparatus includes a first sealed room that receives first crystallizing agent solution, and a communicating tube that communicates with the first sealed room and has a small sectional area for suppressing convection of air. A plurality of droplets of solution dissolving a biological macromolecule and a crystallizing agent therein are held in the communicating tube with the plurality of droplets being separated from each other.

The present invention generally provides multicomponent articles of manufacture and methods of making them. In its broadest aspect, the invention provides a multicomponent article that includes a diffraction grating-based encoded element, wherein the encoded element includes an optical substrate having at least one surface, and an optical coding element; and a substance disposed on at least a portion of the surface of the substrate. The optical substrate may be made from a wide variety of materials. Importantly the multicomponent article may be a reagent particle wherein the substance includes a reagent. The reagent may be chosen from a wide range of biological macromolecules and oligomeric molecules, from any organic chemical or inorganic chemical compound including pharmaceutical agents and candidate pharmaceutical agents, modifications of any of them, and from any microbiological entity, a cell, and similar entities. In another aspect the invention provides a coded reagent library including a plurality of reagent particles described herein the preceding paragraphs. In another aspect the invention provides a method of preparing a multicomponent article including the steps of providing a diffraction grating-based encoded element, and binding a substance to a surface of said optical substrate. The invention also provides a method of preparing a coded reagent library. Additionally the invention provides a method of synthesizing a polynucleotide reagent on a multicomponent article.

The invention discloses a biomacromolecule interpenetrating polymer network hydrogel and a preparation method of the biomacromolecule interpenetrating polymer network hydrogel. The biomacromolecule interpenetrating polymer network hydrogel is formed by crosslinking two kinds of enzymes in a catalysis mode, one is a polysaccharide macromolecule network formed by crosslinking polysaccharide macromolecules with introduced phenolic hydroxyl groups in an oxidation mode as oxidase and hydrogen peroxide catalyze phenolic hydroxyl groups, and the other is a protein or polypeptide macromolecule network formed crosslinking protein or polypeptide containing amino acid residues as transferase catalyzes peptide bonds. The two networks interpenetrate each other and form the novel interpenetrating polymer network hydrogel without bonding of chemical bonds. No chemical crosslinking agent is used in the hydrogel, and the hydrogel has excellent biocompatibility and mechanical property, can be shaped like a dry or wet film, like porous sponge or fibers, and can serve as a contact lens, a medicine release carrier, a scaffold for tissue engineering or materials for tissue repair.

The subject invention provides novel and efficacious systems and methods for particle, chemical, and / or biocompound sensing. In one embodiment, the system of the invention comprises a sensing device that includes a membrane containing at least one nanochannel that spans all or substantially all of the thickness of the membrane. The nanochannel(s) of the invention can be functionalized to enhance target analyte detection and quantification. In one embodiment, the nanochannel is conically shaped and includes a molecular recognition agent for a target analyte. In certain operations, the sensing systems of the invention quantitatively and qualitatively detect biochemical / biomedical species and biomacromolecules, such as proteins, DNA, cells, spores and viruses, with a high degree of sensitivity and specificity.

The invention is directed toward systems and methods for the formation of two dimensional monolayer structures of ordered biomacromolecules, such as viruses, atop cohesive polyelectrolyte multilayers to create functional thin films. Methods for the formation of such thin films are disclosed that involve an interdiffusion-induced assembly process of the biomacromolecules. The inventive systems provide a general platform for the systematic incorporation and assembly of organic, biological and inorganic materials and will enable many potential technological applications such as, for example, chemical and biological sensors, power devices and catalytic membranes.

The invention discloses a bio-macromolecular hydrogel prepared by enzyme catalysis and ion cross-linking, and a preparation method of the bio-macromolecular hydrogel. The bio-macromolecular hydrogel comprises a protein or polypeptide bio-macromolecular network formed by enzyme-catalyzed cross-linking of protein or polypeptide or amino acid residue-containing molecule, accounting for 1-99 wt% of total dry mass of the hydrogel; and a polysaccharide bio-macromolecular network formed by bivalent ion cross-linking of polysaccharide macromolecule, accounting for 1-99 wt% of total dry mass of the hydrogel. The above two networks are inter-penetrated without chemical bonding. The hydrogel has the advantages of excellent mechanical properties, no use of chemical cross-linking agent, simple and effective preparation method, good bio-compatibility and mechanical strength, and capability of steam sterilization; may be in the forms of wet or dry film, porous sponge, tube and particles; and can be used in cell / tissue culture, and used as tissue repair material, tissue engineered scaffold or drug release carrier.

The invention discloses a preparation method of self-adhesion electroconductive hydrogel and belongs to the technical field of biomaterial preparation. The preparation method includes following steps: introducing double bonds on biomacromolecules through methyl acrylic anhydridization to obtain double-bond biomacromolecules; preparing a polydopamine-functionalized electroconductive nano material, and polymerizing the double-bond biomacromolecules and the polydopamine-functionalized electroconductive nano material through photo-initiated free radicals to obtain the self-adhesive electroconductive hydrogel. With reference to adhesiveness of mussel-imitated materials, phenolic hydroxyl functional group with adhesiveness is introduced, and regulation of self-adhesion performance of the hydrogel is realized by changing content of the phenolic hydroxyl functional group. In addition, the self-adhesion electroconductive hydrogel prepared by the method has high electroconductivity, mechanical performance and biocompatibility, and application range of the hydrogel is widened. The preparation method has the advantages of being simple and easy to implement, controllable in polymerization process, high in polymerization speed and low in energy consumption.

Disclosed are apparatus and methods for enhancing or improving cell cultures, including cell cultures for the production of monoclonal antibodies, using electromagnetic energy treatment, primarily using light in the near infrared to visible region of the spectrum. The delivery of light energy to a culture, in accordance with preferred embodiments, enhances or improves the cell culture such as by providing for enhanced and accelerated formation of important biological macromolecules, including, but not limited to, antibodies, proteins, collagen, and polysaccharides, and also providing for accelerated cellular replication and an enhancement or prolongation of the life of cells so treated.

The present invention relates generally to compositions, methods and kits for use in clarification and viscosity reduction of biological samples. More specifically, the invention relates to such compositions, methods and kits that are useful in the isolation of biological macromolecules from cells (e.g., bacterial cells, animal cells, fungal cells, viruses, yeast cells or plant cells) via lysis and one or more additional isolation procedures, such as one or more filtration procedures. In particular, the invention relates to compositions, methods and kits wherein biological macromolecules are isolated using a filter, where the pore size increases in the direction of sample flow. The compositions, methods and kits of the invention are suitable for isolating a variety of forms of biological macromolecules from cells. The compositions, methods and kits of the invention are particularly well-suited for rapid isolation of nucleic acid molecules from bacterial cells.

Disclosed is a nervous tissue engineering tubular supporting stand and method for making same, wherein the nervous tissue engineering tubular supporting stand comprises a chitosan pipe wall and biological source filling base material with axial multiple passages, and the method consists of preparing semipermeable chitosan hollow pipe with 1-5mm of inside diameter, pouring large biological molecule solvent such as chitosan, collagen or gelatine, and utilizing special-purpose die arrangement and lyophilization technology. The obtained multiple-pass nerve channel with bionic construction is beneficial for cell adhesion, migration and leading of neuraxon directional growth, and suitable of the renovation and regeneration of nerve damages.

The invention provides super macroporous polymer microspheres and a preparation method thereof. The preparation method comprises the following steps of: firstly, preparing an oil-in-water in-water composite emulsion as a template for super macroporous microspheres through a two-step emulsion process; then, solidifying an oil phase by using a solvent removal method to form super macroporous microspheres provided with inner-outer through pore passages; and finally, after molding the microspheres, further crosslinking microsphere skeleton molecules to obtain microspheres with rigid resin structures. The microspheres prepared by the method have a through pore passage structure, the controllable particle size range is 0.1-300 microns, the controllable pore size range is 0.09-90 microns, and the controllable porosity range is 10-90%. Super macroporous structures are beneficial for biological macromolecules to penetrate through and enter the microspheres, the mass transfer by convection in the microspheres can be realized, and the rigid structure can tolerate higher pressure and higher flow velocity. The super macroporous polymer microspheres can be used as stationary phase fillers for chromatographic separation, immobilized carriers of enzymes, cell culture micro-carriers, tissue engineering micro scaffold materials, adsorbing materials and the like.

A series of MOF-based hierarchical porous material, namely IPD-mesoMOF-1˜9, based on nanoscale MOFs of MIL-100(Al, Fe, Cr, Sc and In), MIL-53(Al), HKUST-1, DUT-5, DUT-4, MIL-101(Cr), MIL-101NDC(Cr), MIL-101BPDC(Cr) and MIL-110 respectively, forming the permanent interparticle porosities by using close (or relatively close) packing, and preparation methods thereof. Modulated or functionalized IPD-mesoMOFs can be applied for gas adsorption and molecule separation (such as CH4- and CO2-adsorption, gasoline / diesel desulfurization and purification), catalyst loadings and molecular recognition / immobilization of biological macromolecules and enzymes.

The invention discloses a polymer micro-needle array chip. The chip comprises a micro-needle array and a substrate used for the standing of the micro-needle array; and the matrix material of the micro-needle array is a polyacrylamide polymer. The invention also discloses a preparation method of the polymer micro-needle array chip, a micro-needle transdermal drug delivery patch prepared through utilizing the polymer micro-needle array chip, and a preparation method of the patch. The micro-needle of the polymer micro-needle array chip has a high mechanical strength and a sharp needle point, so the skin cuticle can be easily pierced; the preparation method avoids a high-temperature processing treatment step, and is in favor of maintaining the activities of biomacromolecules comprising polypeptides, proteins and the like; the polyacrylamide polymer can easily dissolve or swell after meeting with the water-containing environment, and is in favor the sustained release of drugs in skins; and the preparation method of the polymer micro-needle array chip based transdermal drug delivery patch is simple, so the batch produced can be realized through utilizing present manure processing technologies.

Disclosed are products and methods to facilitate the identification of compounds that are capable of interacting with biological macromolecules of interest, especially when such macromolecules are attached to a support surface in microarray. Aspects of the invention concern attachment chemistry, peptide labeling, antibody preparation, applications and so on.

A novel sorbent suitable for use as a stationary phase in a chromatography column, the core of which consists of an organic polymer of synthetic or natural origin. Further, the carrier exhibits a plurality of covalently bonded non-aromatic zwitterionic groups on its surface. Additionally, the invention also relates to a method for purifying a particular biological macromolecule, such as a protein or a nucleic acid, by zwitterionic ion exchange chromatography as well as an ion exchange column suitable for use in the zwitterionic ion exchange chromatography.

The invention relates to a growth factor slow-release type double-layered artificial skin which is used for repairing injured skin with related growth factor slow-release functions and a preparation method thereof. The artificial skin is divided into two layers of an epidermal layer and an enderonic layer, wherein the epidermal layer is constituted by a micropore double-layered thin film with the functions of water proofing, ventilation and enderonic protection; the materials of the upper layer which contacts with air is polyurethane, or silicon rubber, or polyethylene glycol, or ethylene terephthalate and other medical materials; the lower layer which contacts with the dermis is a natural biological macromolecule thin film which is constructed by silk fibroin and chitosan; the enderonic layer of the artificial skin is constituted by collagen, polysaccharide and microspheres carrying growth factors related to skin regeneration and repairing; the microspheres have good slow-release function, and the slow-release period is equal to or slightly longer than the degradation period of the collagen and polysaccharide and can be matched with the skin regeneration and repairing period; and the thickness of the artificial skin is in a range of 0.5-1.5mm and can be regulated according to requirements.

The various embodiments herein provide an electrospun fiber mat filter comprising a cigarette filter containing macrocycle for removing toxic compounds from a toxic material, wherein the toxic material comprises liquid, gas, and cigarette smoke and a method of synthesizing the same. The electrospun fiber mat cigarette filter comprises a biological, organic or synthetic macrocycle, plurality of additives, a solvent and an acceptable polymeric carrier. The biological macromolecules are engineered polyhemoglobin and / or chlorophyll. The biological, organic, or synthetic macrocycle are electrospun with the acceptable polymeric carrier in presence of an abruptly asymmetric electric field to form an electrospun fiber mat. The electrospun fiber mat is made up of networks of plurality of nanofibers.

Electrospray systems and modified electrospray systems for the fabrication of core-shell particles for controlled-release and / or sustained-release treatment and delivery are herein disclosed. The electrospray system may include between one and a plurality of co-axially situated tubes. Each tube may be electrically connected to a power supply wherein a voltage may be applied thereto. Core-shell particles may be collected on a collection target, which may be a wet or dry collector, and electrically connected to the power supply. Core-shell particles and methods of manufacture are also disclosed. The precursors of the core-shell particles may be polymer- or biomacromolecule-based solutions and may include at least one treatment agent incorporated therein. The number of “core” particle(s) within the “shell” may vary and may provide different treatment agent release profiles depending on the material and / or chemical characteristics of the polymer and / or biomacromolecule used. Methods of treating a condition are also disclosed. A treatment may include delivery of a plurality of core-shell particles which include a treatment agent to a treatment site. Delivery may be performed by a surgical procedure or by a non-invasive procedure such as catheter delivery.

The invention relates to a method for preparing an NCC / CS / PVA composite nanofiber membrane. NCC is rod-shaped particles, wherein the diameter of each particle ranges from 20 nm to 60 nm, and the particles are highly crystallized. The method comprises the steps that (1) an NCC / CS solution is prepared, wherein the concentration of the NCC, by weight, ranges from 0.09% to 0.21%; (2) an NCC / PVA solution is prepared, wherein the concentration of the NCC, by weight, ranges from 0.4% to 0.8%; (3) an acetic acid solution, tetraethyl orthosilicate, the NCC / CS solution obtained in the step (1) and processed through ultrasound, and the NCC / PVA solution obtained in the step (2) and processed through ultrasound are mixed to obtain a spinning solution, and the nanofiber membrane is obtained through the electrospinning technology, wherein the mass ratio of the acetic acid solution to the tetraethyl orthosilicate to the NCC / CS solution to the NCC / PVA solution is (2.5-3.5):(1.5-2.5):5:5; (4) the obtained nanofiber membrane is immersed in an alkaline solution for 2-6 hours at the normal temperature, and the membrane structure can be stable. The method for preparing the NCC / CS / PVA composite nanofiber membrane has the advantages that the nanofiber material preparation process is simple, the preparation process is environmentally friendly and free of pollution, nanofibers are excellent in mechanical performance, the surface of the nanofiber membrane is rich in modifiable functional groups, and the nanofiber membrane has a remarkable affinity effect on biomacromolecule.

The present invention discloses a graphene matrix and an application of the graphene matrix in matrix-assisted laser desorption / ionization-time of flight-mass spectrometry (MALDI-TOF-MS) detection. According to the present invention, in the MALDI-TOF-MS, the graphene is adopted as an assisted matrix, such that the analysis and the detection of the materials including from the small molecule compounds to the biological macromolecules can be efficiently and rapidly achieved; importantly the background interference of the molecular ion peak of the traditional organic matrix can be effectively eliminated so as to achieve the analysis and the detections of amino acids, lipid compounds, peptides, proteins, oligonucleotides, and other structural molecules; the MALDI-TOF-MS adopting the graphene as the matrix is the desorption ionization method, which does not require addition of any organic matrixes, such that the decomposition of the analyte can be avoided, and good reproducibility and high salt tolerance are provided; the efficient and rapid detection method is provided for the natural products and the biological metabolites; the method further can be used for the detection of the common biological macromolecules, and can be popularized and applied in all the MALDI-TOF mass spectrometry so as to achieve the deep application of the graphene.

The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The invention is characterized by the use of low dead volume columns, which is achieved in part by the use of low pore volume frits (e.g., membrane screens) to contain a bed of extraction media in the column. Low dead volume facilitates the elution of the captured analyte into a very small volume of desorption solution, allowing for the preparation of low volume samples containing relatively high concentrations of analyte.

The present invention provides a preparation method of supermacroporous polymer microsphere and its product. It is characterized by that said preparation method includes the following steps: adding high-content surfactant into oil phase containing monomer, making the oil phase containing monomer and surfactant be dispersed in water phase, making the above-mentioned material undergo the process of suspension polymerization so as to obtain supermacroporous microsphere. The grain size of said microsphere is 1-200 micrometers, and its porosity is 10%-90%.

The invention belongs to the technical field of bio-macromolecular pharmaceutical preparations, and specifically relates to a DNA, a plasmid and a preparation capable of directionally clearing HBV (Hepatitis B virus) ccc in hepatocyte. According to the invention, five brand-new CRISPR / Cas systems are discovered through wide and in-depth studies, and can effectively clear HBV ccc DNA in cells, inhibit reproduction of HBVs, and reduce expression of proteins related to the HBVs. A pH-sensitive and PEG-modified cationic lipid carrier is further prepared, and has the advantages of good stability and high transfection efficiency; and internal instability of multiple cationic lipid carriers is overcome. The CRISPR / Cas systems are combined with the pH-sensitive and PEG-modified cationic lipid carrier to prepare a CRISPR / Cas9 cationic lipid carrier preparation which can effectively inhibit virus reproduction in a murine model suffering from acute HBV infection and reduce antigen expression level.

The invention provides an efficient pig-feed complex enzyme preparation with enzyme protectant. The efficient pig-feed complex enzyme preparation with enzyme protectant is capable of promoting absorption of nutrients in the feed by pigs to increase feed utilization rate by degrading antinutritional factors, such as beta-glucan, phytic acid and pectin, biomacromolecules and the like. By adding the protectant, inactivity rate in enzymes can be reduced, effective time can be prolonged, and the effects of the enzyme preparation are more evident and longer. The efficient pig-feed complex enzyme preparation with enzyme protectant is high in feed conversion rate, feed-to-grain ratio can be lowered by more than 19%, growth speed of pigs is increased by more than 23%, and enzyme activity is kept in 18 months and can be kept by more than 90%.

The temperature sensitive hydro gel medicine releasing system and its preparation process belongs to the field of medicine technology. The medicine releasing system consists of carried medicine 0.1-30 wt%, temperature sensitive hydro gel 20-90 wt%, and high molecular weight polysaccharide 5-50 wt%. The medicine releasing system is prepared through a polysaccharide-temperature sensitive hydro gel compounding process, a polysaccharide-temperature sensitive hydro gel hydrophilic emulsifying process or a polysaccharide-temperature sensitive hydro gel emulsifying process. Owing to the retarding diffusion effect during solution-gel conversion, the present invention has raised medicine carrying amount, and improved medicine stability. The present invention provides simple and practical technological scheme for the controllable conveyance of biological macro molecular medicine and other treating components and the improvement of carried medicine release.

The invention relates to magnetic fluorescent microspheres and a preparation method thereof. The particle size of the magnetic fluorescent microspheres is 5-10 mu m, the fluorescence excitation wavelength range is 400-700nm, and the fluorescence intensity is not reduced within 24h. The prepration method comprises the following steps: adding a swelling agent into monodisperse carboxylated polystyrene microspheres, and adding magnetic nano microparticles into a swelling system; shaking on a decolorization shaker for 12-48h; using mixed solution of cyclohexane and ethanol for cleaning sediment, and sequentially carrying out ultrasonic dispersion and centrifugal separation till supernatant liquid is colorless under an ultraviolet lamp; and saving a final sediment product in 1ml of liquor. Compared with the existing magnetic fluorescent microspheres, the magnetic fluorescent microspheres have the advantages of uniform and controllable diameter, good fluorescence stability, simple preparation process, multiple types of fluorescence codes and the like, and can not only carry out fast separation and purification on reactants by being applied in the biological macromolecular detection, but also simultaneously detect a plurality of target molecules in a sample to be detected in a reaction tube and a hole, thereby being widely applied in the fields of immunoassay, nucleic acid hybridization, genotype analysis and the like.

The invention relates to the technical field of ordered porous materials, in particular to a highly ordered porous silica and a porous silicon carbonitride material and a preparation method and application thereof. Under a certain temperature, egg white adopted as template and structure-directing agent is stirred and then hydrolytically polycondensed through oil-soluble silicon source capable of controlling hydrolysis, and the template is removed from the obtained mixture under different roasting conditions, so that the ordered porous silica and the ordered porous silicon carbonitride material can be respectively obtained. The two materials have the unique ordered structure of the egg white, and have a specific surface area and good heat stability. Compared with the conventional method, the invention has the advantages of low cost, simple preparation technique, environment-friendliness and the like, and the obtained materials have a broad application prospect in fields such as the controlled release, separation, adsorption and catalysis of large biological molecules and proteins.