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Magnetic fluorescent microspheres and preparation method thereof

A technology of fluorescent microspheres and magnetism, which is applied in the fields of microsphere preparation, microcapsule preparation, chemical instruments and methods, etc. It can solve the problem of the reduction of fluorescence performance of magnetic fluorescent microspheres, the reduction of flow cell signal accuracy, and the limitation of magnetic fluorescent microspheres. Application and other issues, to achieve uniform and controllable particle size, good photochemical stability, and various types of fluorescent codes

Inactive Publication Date: 2010-07-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole process needs to go through many steps, which increases the production cost and limits the application of magnetic fluorescent microspheres
[0005] 2. The prepared microspheres have poor performance
Due to the addition of magnetic nanoparticles in the polymerization process of the microspheres in the usual preparation method, the surface of the prepared microspheres is rough and the particle size uniformity is poor, which reduces the accuracy of the signal during the flow cytometry detection process.
The organic fluorescent dyes used at the same time have disadvantages such as poor stability and easy quenching of fluorescence, which greatly reduce the fluorescence performance of magnetic fluorescent microspheres.

Method used

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  • Magnetic fluorescent microspheres and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] (1) Accurately weigh 90 mg of carboxylated polystyrene microspheres with a particle size of 4 microns and put them in a centrifuge tube, add 5 ml of n-butanol and chloroform with a volume ratio of 1:1 as a swelling agent, ultrasonic Disperse and spare.

[0037] (2) Use a pipette to accurately measure 5 mg of quantum dots with a particle size of 7 nm and an emission wavelength of 500 nm and 5 mg of magnetic nanoparticles with a particle size of 5 nm and a saturation magnetization of 73 emu / g, and add them to the swelling system.

[0038] (3) Place the composite system of quantum dots, magnetic nanoparticles, and carboxylated polystyrene microspheres on a decolorizing shaker and shake for 36 hours.

[0039] (4) Stop the reaction, centrifuge, use 2ml of cyclohexane-ethanol mixed solution (the volume ratio of cyclohexane to ethanol is 3:1), wash the precipitate, after ultrasonic dispersion, centrifuge until the supernatant is colorless.

[0040] (5) The final precipitation product...

Embodiment 2

[0042] (1) Accurately weigh 98 mg of carboxylated polystyrene microspheres with a particle size of 8 microns and put them into a centrifuge tube, add 5 ml of n-butanol and chloroform with a volume ratio of 1:2 as a swelling agent, ultrasonic Disperse and spare.

[0043] (2) Use a pipette to accurately measure 1 mg of quantum dots with a particle size of 4 nm and an emission wavelength of 610 nm and 1 mg of magnetic nanoparticles with a particle size of 7 nm and a saturation magnetization of 60 emu / g, and add them to the swelling system.

[0044] (3) Place the composite system of quantum dots, magnetic nanoparticles, and carboxylated polystyrene microspheres on a decolorizing shaker and shake for 24 hours.

[0045] (4) Stop the reaction, centrifuge, wash the precipitate with 2ml of cyclohexane-ethanol mixed solution (the volume ratio of cyclohexane to ethanol is 1:1), after ultrasonic dispersion, centrifuge until the supernatant is not under UV light color.

[0046] (5) The final prec...

Embodiment 3

[0048] (1) Accurately weigh 96 mg of carboxylated polystyrene microspheres with a particle size of 10 microns and place them in a centrifuge tube, add 5 ml of n-butanol and chloroform with a volume ratio of 1:3 as swelling agents, and ultrasonic Disperse and spare.

[0049] (2) Use a pipette to accurately measure 2 mg of quantum dots with a particle size of 10 nm and an emission wavelength of 400 nm and 2 mg of magnetic nanoparticles with a particle size of 8 nm and a saturation magnetization of 80 emu / g, and add them to the swelling system.

[0050] (3) Place the composite system of quantum dots, magnetic nanoparticles, and carboxylated polystyrene microspheres on a decolorizing shaker and shake for 48 hours.

[0051] (4) Stop the reaction, centrifuge, wash the precipitate with 2ml of cyclohexane-ethanol mixed solution (the volume ratio of cyclohexane to ethanol is 2:1), after ultrasonic dispersion, centrifuge until the separated supernatant is in the ultraviolet Colorless under th...

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Abstract

The invention relates to magnetic fluorescent microspheres and a preparation method thereof. The particle size of the magnetic fluorescent microspheres is 5-10 mu m, the fluorescence excitation wavelength range is 400-700nm, and the fluorescence intensity is not reduced within 24h. The prepration method comprises the following steps: adding a swelling agent into monodisperse carboxylated polystyrene microspheres, and adding magnetic nano microparticles into a swelling system; shaking on a decolorization shaker for 12-48h; using mixed solution of cyclohexane and ethanol for cleaning sediment, and sequentially carrying out ultrasonic dispersion and centrifugal separation till supernatant liquid is colorless under an ultraviolet lamp; and saving a final sediment product in 1ml of liquor. Compared with the existing magnetic fluorescent microspheres, the magnetic fluorescent microspheres have the advantages of uniform and controllable diameter, good fluorescence stability, simple preparation process, multiple types of fluorescence codes and the like, and can not only carry out fast separation and purification on reactants by being applied in the biological macromolecular detection, but also simultaneously detect a plurality of target molecules in a sample to be detected in a reaction tube and a hole, thereby being widely applied in the fields of immunoassay, nucleic acid hybridization, genotype analysis and the like.

Description

Technical field [0001] The invention relates to a magnetic fluorescent microsphere and a preparation method thereof. Background technique [0002] Magnetic fluorescent microspheres refer to the introduction of magnetic nanoparticles and fluorescent substances into polymer microspheres, so that the microspheres have both magnetic properties and characteristic fluorescence, and can be identified and analyzed by a flow cytometer. The surface of the magnetic fluorescent microspheres can bind various biomolecules. These biomolecules react with the corresponding target molecules in the sample, and use fluorescently labeled assay substrates as the reporter group for detecting biological reactions. Qualitative and quantitative analysis of target molecules. Magnetic fluorescent microspheres have a very broad range of biomedical applications. The use of magnetic fluorescent microspheres for biological macromolecule detection can not only quickly separate and purify the reactants, but als...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L25/08C08L33/02C08K3/30C08K3/22C09K11/88C09K11/56H01F1/42B01J13/02
Inventor 王汉杰宫晓群宋涛苏文雅常津
Owner TIANJIN UNIV
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