The invention provides super macroporous
polymer microspheres and a preparation method thereof. The preparation method comprises the following steps of: firstly, preparing an oil-in-water in-water composite
emulsion as a template for super macroporous microspheres through a two-step
emulsion process; then, solidifying an
oil phase by using a
solvent removal method to form super macroporous microspheres provided with inner-outer through pore passages; and finally, after molding the microspheres, further crosslinking
microsphere skeleton molecules to obtain microspheres with rigid resin structures. The microspheres prepared by the method have a through pore passage structure, the controllable particle size range is 0.1-300 microns, the controllable pore size range is 0.09-90 microns, and the controllable
porosity range is 10-90%. Super macroporous structures are beneficial for biological macromolecules to penetrate through and enter the microspheres, the
mass transfer by
convection in the microspheres can be realized, and the
rigid structure can tolerate higher pressure and higher flow velocity. The super macroporous
polymer microspheres can be used as
stationary phase fillers for
chromatographic separation, immobilized carriers of enzymes,
cell culture micro-carriers,
tissue engineering micro
scaffold materials, adsorbing materials and the like.