PCT No. PCT / GB96 / 01830 Sec. 371 Date Apr. 21, 1998 Sec. 102(e) Date Apr. 21, 1998 PCT Filed Jul. 25, 1996 PCT Pub. No. WO97 / 05280 PCT Pub. Date Feb. 13, 1997The invention relates to the detection of target nucleic acids or
nucleic acid units in a sample, by obtaining a SER(R)S spectrum for a SER(R)S-active complex containing, or derived directly from, the target. The complex includes at least a SER(R)S-active
label, and optionally a
target binding species containing a
nucleic acid or
nucleic acid unit. In this detection method, the concentration of the target present in the SER(R)S-active complex, or of the nucleic acid or unit contained in the
target binding species in the SER(R)S-active complex, is no higher than 10-10 moles per
liter. Additionally or alternatively, one or more of the following features may be used with the method: i) the introduction of a
polyamine; ii) modification of the target, and / or of the nucleic acid or nucleic acid unit contained in the
target binding species, in a manner that promotes or facilitates its chemi-
sorption onto a SER(R)S-
active surface; iii) inclusion of a chemi-sorptive functional group in the SER(R)S-active
label. The invention also provides SER(R)S-active complexes for use in such a method, a kit for use in carrying out the method or preparing the complexes and a method for sequencing a nucleic acid which comprises the use of the detection method to detect at least one target
nucleotide or sequence of nucleotides within the acid.