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644results about How to "Avoid structural damage" patented technology

Inductive RF plasma source with external discharge bridge

An RF ICP source having a housing with a flanged cover. The interior of the housing serves for confining plasma generated by the plasma source. The cover has at least two openings which are connected by a hollow C-shaped bridge portion which is located outside the housing. The hollow C-shaped bridge portion is embraced by an annular ferrite core having a winding connected to an electric power supply source for generating a discharge current which flows through the bridge portion and through the interior of the housing. The discharge current is sufficient for inducing plasma in the interior of the housing which is supplied with a gaseous working medium. The power source operates on a relatively low frequency of 60 KHz or higher and has a power from several watt to several kilowatt. In order to provide a uniform plasma distribution and uniform plasma treatment, the cover may support a plurality of bridges. Individual control of the inductors on each bridge allows for plasma redistributing. The housing of the working chamber can be divided into two section for simultaneous treatment of two objects such as semiconductor substrates. A plate that divides the working chamber into two sections may have ferrite cores built into the plate around the bridges. In another embodiment, the flow of gaseous working medium is supplied via a tube connected to the bridge portion of the source.
Owner:ADVANCED ENERGY IND INC

Resilient thimble for ball head of prosthetic joint

A head component for use in a prosthetic joint such as a hip joint. The head has a body adapted to engage a natural acetabulum or a prosthetic acetabular cup component. The head has a recess with a resilient sleeve located therein adapted to engage the trunnion of a prosthetic stem component with which it is to be used. The sleeve material is more deformable than the trunnion material and can deform under sufficient force to absorb any unevenness in the trunnion surface.
Owner:BENOIST GIRARD & CIE

Laser unsealing method

The invention discloses a laser unsealing method. The method includes the steps that positions and depths of all elements and a chip in a plastic package device are positioned to obtain size, position and depth information of the elements and the chip in the device; according to the information, a preset unsealing area of the plastic package device is determined for unsealing and positioning; laser ablation is conducted on the plastic package device in a cross filling mode to remove molding materials on the surface of the plastic package device, so that inner lead bonding wires are just exposed out of a chip area, tin-lead welding points are just exposed near a resistor and a capacitor, and inductance is just exposed near an inductor. Acidic liquid is adopted to corrode a molding compound covering the chip till the chip is exposed. The method can well solve the problem that a traditional method can not unseal a plastic mixed integrated circuit easily, and effectively increases the unsealing success rate of the plastic package device.
Owner:CASIC DEFENSE TECH RES & TEST CENT

Method for strengthening and toughening biological ceramic material by use of graphene and preparing artificial bone prepared from material

Aiming at the problems of low strength and low toughness of bioactive ceramics, the invention provides a method for strengthening and toughening a bioactive ceramic material by use of graphene and preparing graphene / biological ceramic artificial bone by a selective laser sintering (SLS) technology. The method has the advantages that graphene is added into the bioactive ceramic (hydroxylapatite, calcium phosphate, bioglass, calcium silicate and the like) as a nano enhanced phase, and the strength and toughness of the ceramic can be remarkably improved by use of the excellent mechanical property of graphene; the SLS technology is applied to the preparation process of graphene / biological ceramic artificial bone, and the sintering time can be shortened to the second level and even millisecond level by use of the characteristic of quick heating and cooling of laser so as to avoid structural injury of graphene caused by long-time high-temperature effect. The method for strengthening and toughening the ceramic material by use of graphene provided by the invention is of great significance to the application of bioactive ceramic to the load bearing parts of the human body.
Owner:CENT SOUTH UNIV

Glycol catalyst prepared by dimethyl oxalate hydrogenation and preparation method thereof

The invention discloses a glycol catalyst prepared by dimethyl oxalate hydrogenation and a preparation method thereof, which is characterized in that the catalyst takes copper nitrate or copper acetate as copper source, ludox as silicon source, ammonia, ammonium bicarbonate or urea as precipitant which are coprecipitated to prepare CuSi catalyst forerunner; one or two of Zn, Fe, Ag, and Pd is soaked in the catalyst forerunner and used as additive agent, and then mixture is baked in the temperature of 200 to 500 DEG C, and finally is beaten into pieces and formed to obtain the catalyst. The catalyst comprises 20-50% of Cuo, 0.005%-3% of additive agent and the rest of SiO2 according to mass percent of oxygen compound, wherein additive agent is oxidate of one or two of Zn, Fe, Ag, and Pd. Before using, the catalyst is reduced by hydrogen and nitrogen combination gas containing 1% to 90% of hydrogen; and the reduction temperature is 150 to 500 DEG C.
Owner:HAISO TECH

Buckling-restrained shearing steel plate energy dissipation device with clamping plates

InactiveCN103195184AReduced ductilityGuaranteed Yield Energy DissipationShock proofingPunchingEngineering
The invention discloses a buckling-restrained shearing steel plate energy dissipation device with clamping plates. The energy dissipation device comprises a core energy dissipation component, a buckling-restrained device, a top plate and a bottom plate, the core energy dissipation component is a rectangular energy dissipation steel plate, the top plate and the bottom plate are fixedly connected at the upper end and the lower end of the rectangular energy dissipation steel plate respectively, the buckling-restrained device comprises two clamping plates, four guide trough plates and stiffening ribs, the two clamping plates are arranged on the front side and the rear side of the rectangular energy dissipation steel plate respectively, the upper ends of the two clamping plates are fixed with the top plate, the four guide trough plates are fixed on the bottom plate and limit movement of the lower ends of the two clamping plates respectively, and the stiffening ribs are arranged on one lateral surface, departing from the rectangular energy dissipation steel plate, of each clamping plate. By the buckling-restrained shearing steel plate energy dissipation device with the clamping plates, slotting or punching of the core energy dissipation component can be avoided, and the stiffening ribs can be prevented from being directly welded on the core energy dissipation component. By the aid of the buckling-restrained device, out-of-plane deformation of the core energy dissipation component can be limited effectively, bearing capacity is enhanced, and hysteretic behavior is improved.
Owner:江苏力汇振控科技有限公司

Frame assembly for the opening of a structure

A frame assembly may be secured within an opening in a structure and is adapted to carry a window assembly therein. The frame assembly may include a pair of spaced vertical rails and a top rail secured to a top end of the vertical rails. A bottom rail may be secured to a bottom end of the vertical rails and the window assembly is removable without damaging the underlying structure.
Owner:WAYNE DALTON CORP +1

Method for fixing seabed pipeline and preventing buckle propagation

The invention belongs to the technical field of seabed pipeline laying and particularly relates to a method for fixing a seabed pipeline and preventing buckle propagation. The method comprises: manufacturing a plurality of pairs of reversed U-shaped shells of which the inside diameters are the same and the outer edges are tangent according to the outside diameter and wall thickness specification of the seabed pipeline, wherein the upper parts of the shells are in semi-annular shapes and the lower parts of the shells have semi-cylindrical hollow cuboid bases; connecting the surfaces of the bases with barrel structures to form buckle preventing fixers; and mounting the fixers on the seabed pipeline at intervals along a radial direction. The method adopts a reasonable mounting process, and can simplify operation, reduce cost, effectively fix the seabed pipeline and prevent buckle propagation, and ensure the safe and stable operation of the seabed pipeline.
Owner:TIANJIN UNIV

Shoe with energy storage and delivery device

InactiveUS7510538B2Improving surface gripImproving impact dampingSolesNon-surgical orthopedic devicesTibiaEngineering
According to the invention, a shoe having at least one base spring element, which is arranged between appendages at a heel zone of the shoe and at a shaft zone taking support at the front edge of the shin bone and which stretches in the course of an ambulation phase, characterized in that a tensioning assembly moves the appendage of the base spring element at the heel zone away from the appendage at the shaft zone upon planting of the shoe, for stretching of the base spring element.
Owner:DIETMAR WOLTER

Asphalt surface treatment

A method and composition for treating a weathered low traffic volume asphalt surface. The method comprising the steps of providing a composition comprising a solution, emulsion or dispersion of a polymer material, particulate material, rheology modifiers, processing aids, and UV absorbers, wherein the composition is essentially free of bituminous components and is essentially free of cement; and applying the composition to the asphalt surface. The composition preferably is a shear thinning formulation which exhibits a marked reduction in viscosity when sprayed or otherwise subjected to shear during application but increases in viscosity after application.
Owner:ROADSAVE HLDG

Lightweight melamine aerogel felt and preparation method thereof

The invention provides a lightweight melamine aerogel felt and a preparation method thereof. The lightweight melamine aerogel felt is characterized by comprising, by volume, 99.4 to 99.7% of SiO2 aerogel and 0.3 to 0.6% of melamine foam, wherein the melamine foam is open-cell foam, the unit weight of the foam is 4 to 10 Kg / m<3>, and the SiO2 aerogel penetrates an interlaced three-dimensional network structure formed by pores of the melamine foam in the form of flocculent gel. The preparation method for the aerogel felt comprises the following successive steps: (1) preparing a silica sol system; (2) putting the melamine foam into a die, casting the silica sol system and then carrying out standing and aging; and (3) successively carrying out cleaning and displacement, drying and heat treatment so as to obtain the melamine aerogel felt. The aerogel felt has a light weight, good mechanical stability, a regular shape and a smooth surface and is easy to process.
Owner:NANJING UNIV OF AERONAUTICS & ASTRONAUTICS

One-step chemical preparation method for graphene and polyaniline composite materials

The invention discloses a one-step chemical preparation method for graphene and polyaniline composite materials. Hydroiodic acid is served as reductive agent, graphite oxide is served as dopant, and graphite oxide reduction and aniline polymerization are achieved in one step in an acid medium to prepare the graphene and polyaniline composite materials. The one-step chemical preparation method for the graphene and polyaniline composite materials comprises dissolving the graphite oxide into an acetic acid solution, adding aniline monomers, uniformly mixing through ultrasonic dispersion, adding reducing agent hydroiodic acid and dropwise adding ammonium persulfate oxidizing agent, magnetic stirring, achieving aniline in situ polymerization and filtering, washing and drying after complete reaction to obtain the graphene and polyaniline composite materials. According to the one-step chemical preparation method for the graphene and polyaniline composite materials, the graphite oxide in the graphene and polyaniline composite materials is fully restored, generated polyaniline nano-particles are dispersed between graphene sheets, the composition method is simple, the reaction time is short, the composite materials are high in specific surface area and good in reactivity and are conductive composite materials with good flexibility and conductivity.
Owner:ZHONGYUAN ENGINEERING COLLEGE

Display device

A display device is provided, including a front case, a rear case fixed to the front case, a frame disposed between the front and rear cases, a display panel disposed on the frame, and a cushion. The cushion is disposed on the frame and contacts the front and rear cases, so as to space the frame apart from the front and rear cases.
Owner:DELTA ELECTRONICS INC

Electrical connector plug having a metallic shield connected to an electrically conductive housing of the plug

An electrical connector plug having resilient contact terminals electrically connects to an electrical connector socket with a casing and two groups of engaging terminals mounted on or in the casing. The electrical connector plug has an electrically conductive housing. A coupling device extends in a longitudinal direction and has a base portion. Two groups of resilient contact terminals are mounted in the coupling device axially symmetrically arranged with each other in the longitudinal direction, each of which has a flat section and an upwardly protruding contact section. A metallic shield frame is electrically connected to and secured to the electrically conductive housing, having a frame section, a front section, and two lateral protective sections. An electrically conductive section extends from the frame section towards the front section for electrical connection to the casing of the electrical connector socket.
Owner:ADVANCED CONNECTEK INC

Application of carboxymethyl chitosan self-crosslinking polymer in silicon negative electrode of lithium ion battery

The invention discloses an application of a carboxymethyl chitosan self-crosslinking polymer in a silicon negative electrode of a lithium ion battery. The carboxymethyl chitosan self-crosslinking polymer is obtained by adding carboxymethyl chitosan to cross-liking agent 1-(3-(dimethylamino propyl)-3-ethyl-carbodiimide hydrochloride in a self-crosslinking manner; the polymer has a three-dimensional structure, and is taken as a binder to be applied to preparation of the silicon negative electrode of the lithium ion battery; the self-crosslinking polymer shows high mechanical performance and adhesive property; chemical bonds with self-repairing capability are formed between the self-crosslinking polymer and the silicon particles; the silicon negative electrode with large volume change in the charging-discharging process can be effectively accommodated; the powder-fall-off phenomenon with structural damage caused by severe volume changes of the silicon particles in the circulating process of the silicon negative electrode is solved; and a new approach for improving the cycle performance of the silicon negative electrode of the lithium ion battery is provided.
Owner:CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Obstacle-jumping system for quadruped robot

The invention provides an obstacle-jumping system for a quadruped robot. The system comprises a posture detecting subsystem, a motion planning subsystem and a torque control subsystem which are sequentially connected and is characterized in that the posture detecting subsystem processes posture and joint signals returned by the robot and transmits the data to the motion planning subsystem; the motion planning subsystem analyzes topographical change and the size of an obstacle to plan the jump motion trajectory of the robot and calculates the expected displacement, speed and acceleration of therobot; the torque control subsystem calculates the required input torque of each joint according to the planned motion trajectory. By the obstacle-jumping system, when the robot faces a large obstacle or a high step, the robot can jump over the obstacle or jump onto the step.
Owner:SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV

Method for extracting astaxanthin from haematococcus pluvialis

The invention belongs to the technical field of medicines, and relates to a method for extracting astaxanthin from haematococcus pluvialis. The method comprises the steps of culturing a seed stock solution in a glass apparatus with a drainage system, and carrying out later amplification culture of the haematococcus pluvialis and accumulation culture of the astaxanthin after 10-15 d; centrifuging a culture in an exponential growth period of seed culture; and inoculating cell clusters in a BBM basal medium to obtain a primary culture. The later accumulation culture of the astaxanthin is amplification culture by using a breathable plastic bag type simple device provided by the invention. In the accumulation stage, a stress culturing method is adopted to obtain a lab-scale test haematococcus pluvialis culture; and haematococcus pluvialis powder is obtained by spray drying. According to a preparation technology that extracts astaxanthin from the haematococcus pluvialis by adopting an ultrasonic cell disruption assisted mixed solvent extraction method, the haematococcus pluvialis powder is added in an organic solvent to carry out ultrasonic cell disruption, and then the astaxanthin is obtained by the steps of reflux extraction in a water bath, suction filtration, filtrate merging and concentration. Compared with a conventional direct extraction method, the method provided by the invention saves extraction time, and increases astaxanthin yield.
Owner:SHENYANG PHARMA UNIVERSITY

Silicon dioxide aerogel coating and preparation method thereof

The invention discloses a silicon dioxide aerogel coating and a preparation method thereof. The coating is prepared from the following ingredients in parts by weight: 100 parts of silicon dioxide wetgel, 20-50 parts of auxiliary film forming matter, 0.3-0.5 part of mildewproof microbicide and 0.1-2.0 parts of thickening leveling agent. The preparation method comprises the steps of weighing the silicon dioxide wet gel, adding the auxiliary film forming matter, the mildewproof microbicide and a thickener at a low speed with low-speed stirring, carrying out stirring for 30 minutes or more, adding a proper volume of distilled water or deionized water, and adjusting the viscosity of the coating to about 1,500mPa.s, thereby obtaining the finished coating. According to the silicon dioxide aerogel coating and the preparation method thereof, concentrated aerogel colloid is directly used as a raw material, thus, the difficult dispersity of aerogel powder materials is avoided, and the possibility of structural damage to aerogel powder after secondary water absorption is also avoided; meanwhile, through adding a structural stabilizer, the problem of structural collapse during drying is overcome, and a reinforcing effect in a later-stage film forming process can also be achieved; and the coefficient of thermal conductivity of a coating film at normal temperature can reach 0.015W / m.K to theminimum, and the light transmittance of the coating film can reach 80% or above.
Owner:XINHUASHENG ENERGY SAVING TECH CO LTD

Methods and devices for humidity control of materials

This invention provides novel devices and methods for manipulation of moisture / fluid levels in and / or upon objects and is used to, e.g., safely dry wet books and documents with minimal deleterious side effects or to add humidity to such books / documents or other objects or areas.
Owner:US SEC AGRI +1

External fixation clamp

An insert for an external fixation has two jaws for clamping a rod-shaped element. The insert includes a locking element and is an angled piece having two free ends, with at least one engagement edge for engagement into the rod-shaped element being provided at its one free end. The insert has a recess or bore for engagement with a locking shaft of the clamp provided at the free end thereof that lies opposite to the free end having the rod engagement edge. The insert is angled so that it may extend through a passageway in one of the jaws and into a cavity formed in the jaw for receiving the rod. The insert rod engaging edges are tapered to bite into the rod to prevent its movement.
Owner:STRYKER EURO OPERATIONS HLDG LLC

Installation method for thermal insulation and decoration integrated facade panels

The invention relates to an installation method for thermal insulation and decoration integrated facade panels in the field of construction methods of the construction industry. The method includes the following steps: (Step 1) main wall surface treatment; (Step 2) datum line making; (Step 3) framing installation; (Step 4) thermal insulation and decoration integrated facade panel installation; (Step 5) gap filling; (Step 6) sealant filling; (Step 7) panel surface cleaning. When the method disclosed by the invention is adopted, integral installation is convenient, cost is reduced, the construction period is short, the load of facade panels is reduced greatly, the utilizability of space and land is enhanced, the application range is wide, temperature is kept, heat is insulated, energy consumption is reduced, structural destruction caused by the circulation of rain, snow, freezing, thawing, dryness and wetness is prevented, the worry about the water seepage of wall surfaces is eased after installation, the phenomenon that indoor wall surfaces go moldy is effectively prevented, and the thermal insulation and decoration integrated facade panels are environment-friendly and durable.
Owner:杭锦旗亿利朗新材料科技有限公司

Battery fixing mechanism of electronic device

The invention discloses a battery fixing mechanism of an electronic device, the battery fixing mechanism is used for fixing the battery provided with a first combination part to the electronic device, comprising a sliding part which is arranged in the electronic device in a sliding way, an elastic part which provides recovery elasticity for the sliding part, and an operation part which drives the sliding part to slide laterally. The sliding part is provided with a second combination part which is used for combining with the first combination part correspondingly, and a first guide part; the operation part is provided with a second guide part which is corresponding to the first guide part, therefore, on the premise that the operation part moves to a receiving part, the second guide part pushes the first guide part and drives the sliding part to slide laterally, thus leading the second combination part to be disconnected with the first combination part.
Owner:INVENTEC CORP

Axial pre-tightening force double-wave measuring method

The invention provides an axial pre-tightening force double-wave measuring method. The method comprises the steps: integrating a pre-tightening force sensor on one end face of a fastener in situ; determining parameters of the fastener; respectively acquiring transverse wave and longitudinal wave temperature compensation calibration curves of the fastener; measuring a sound time difference from theemission of the transverse wave and the longitudinal wave to the reception of the first echo, and taking the sound time difference ratio of the transverse wave and the longitudinal wave as a reference value; acquiring an axial pre-tightening force calibration curve between the sound time difference ratio of the transverse wave and the longitudinal wave and the axial pre-tightening force of the fastener under the same working condition; and applying a pre-tightening force to the fastener, and measuring the acoustic time difference ratio of the transverse wave to the longitudinal wave to obtainan axial pre-tightening force of the fastener corresponding to the sound time difference ratio. According to the invention, ultrasonic double-wave rapid high-precision measurement of the axial pre-tightening force of the fastener is realized, and problems of poor sensor environmental adaptability, incapability of realizing simultaneous receiving and transmitting of double waves, too redundant detection equipment lines, fuzzy implementation details of the method, measurement failure caused by ultrasonic waveform distortion and the like in the prior art are solved.
Owner:CHENGDU SCI & TECH DEV CENT CHINA ACAD OF ENG PHYSICS

Acoustic excitation non-contact modal testing system and method

The invention belongs to the field of mechanical measurement and provides an acoustic excitation non-contact modal testing system and method. The problem that the conventional modal testing result for a thin-slab structure is inaccurate is solved in a non-contact modal testing mode. According to the technical scheme, the method comprises the following steps: giving a sound by using a loudspeaker, generating vibration through a non-contact excitation thin-slab testing piece, measuring an acoustic signal by using an acoustic sensor, and measuring the vibration response of the thin-slab testing piece by using a scanning-type laser vibration measurement system in a laser non-contact way; by taking the acoustic signal measured by the acoustic sensor as an input signal and taking the vibration response measured by the scanning-type laser vibration measurement system as an output signal, performing analysis through conventional modal analysis software in the scanning-type laser vibration measurement system to obtain modal parameters of the thin-slab testing piece. The system and the method have the benefits that the influence on additional quality and additional rigidity is avoided in a modal test performed by using the system and the method, and the accurate modal parameters can be obtained; meanwhile, the structural damage to the testing piece is avoided, and the testing effect is good.
Owner:BEIJING INST OF STRUCTURE & ENVIRONMENT ENG +1

Fin shaped structure and method of forming the same

ActiveUS20160141387A1Preferable Vth sensitivityAvoid fin structure damageTransistorSolid-state devicesInsulation layerEngineering
A fin shaped structure and a method of forming the same, wherein the method includes forming a fin structure on a substrate. Next, an insulation layer is formed on the substrate and surrounds the fin structure, wherein the insulation layer covers a bottom portion of the fin structure to expose an exposed portion of the fin structure protruded from the insulation layer. Then, a buffer layer is formed on the fin structure. Following this, a threshold voltage implantation process is performed to penetrate through the buffer layer after forming the insulation layer, to form a first doped region on the exposed portion of the fin structure.
Owner:UNITED MICROELECTRONICS CORP

Manhole cover opening and closing movement device

The invention discloses a manhole cover opening and closing movement device which comprises a frame; the two opposite sides of the frame are respectively provided with a rolling device which comprises a step-shaped wheel axle and a wheel axle bracket; the step-shaped wheel axle comprises two cylindrical surfaces with unequal excircle diameters; the smaller cylindrical surface is matched with the upper surface of the frame in a contacting way; the step-shaped wheel axle is provided with an inner hole and is connected with the wheel axle bracket by a pin shaft arranged in the inner hole; the upper part of the rolling device is provided with a catching module; the rolling device is connected with the catching module by a hoisting module; the frame has an angle iron welding structure; the wheel axle bracket of the rolling device has a channel steel structure; a channel steel wall is provided with a screwed hole which is connected with a screw rod by screw thread; the outer edge of the frame is respectively provided with an opening block groove I and an opening block groove II; the position of the opening block groove II is matched with that of the rolling device; and the opening block groove I and the opening block groove II are matched with the screw rod. The manhole cover opening and closing movement device is simple and reliable in structure, convenient to assembled and disassemble and move and suitable for lifting and moving a detection manhole cover which is heavy and inconvenient in traffic.
Owner:国网山东省电力公司超高压公司

Composite structure photonic crystal vibration isolation support

InactiveCN107542827ASolve the singularityIncrease the amount of vibration isolationStands/trestlesShock absorbersSurface typePhonon
The invention relates to a composite structure photonic crystal vibration isolation support. The photonic crystal vibration isolation support is mainly composed of a vibration input shaft sleeve, a vibration isolation lining, a supporting lining, a surface type local resonance type vibration isolation assembly, an embedded type local resonance type vibration isolation assembly, an outer sleeve, alimiting structure and a vibration isolation device base; the vibration input shaft sleeve is connected with the vibration isolation lining, the vibration isolation lining is connected with a bearingshaft sleeve, the bearing shaft sleeve is connected with the outer sleeve through the surface type local resonance type vibration isolation assembly and the embedded type local resonance type vibration isolation assembly, the outer sleeve and the base are connected, and the limiting structure and the base are connected into a whole. The composite structure photonic crystal vibration isolation support solves the problem that a passive type vibration isolation support is narrow in vibration isolation frequency range, single in vibration isolation direction and not obvious in vibration isolationeffect; the mechanism can effectively isolate vertical vibration, lateral vibration and torsional vibration, the limiting effect on large-amplitude vibration can be achieved, and the structure can beused as a vibration isolation device of equipment such as various mechanical devices and precise instruments.
Owner:JILIN UNIV

Electrode and battery using it

An electrode and a battery using the same are provided to improve cycle properties by reducing damages in the structure of active materials caused by repeated expansion-and-shrinkage upon charge and discharge processes and also reducing the reactivity with electrolyte. The electrode comprises: a current collector comprising at least one selected from Cu, Ni, Ti, Fe and Cr; a layer of an active material mounted on the current collector, which comprises at least on selected from Si and Ge compounds; a film layer mounted on the active material layer, which comprises at least one Cu and Ni.
Owner:MURATA MFG CO LTD

Plant pollen tube cell calcium ion ultrastructure positioning method

The invention discloses a plant pollen tube cell calcium ion ultrastructure positioning method which mainly comprises the following steps: (1) preparing a quantitative immobilizing liquid and washing liquid; (2) immobilizing and washing a material; (3) immobilizing and washing secondarily; (4) dehydrating and transiting; (5) carrying out resin permeating, embedding and polymerizing; (6) directly observing by using an electron microscope without dyeing the material. Due to the adoption of the method, the problems that cell turgor pressure needs to be adjusted, abnormal reaction can be caused between potassium pyroantimonate and buffer liquid, calcium pyroantimonate precipitation displacement or dilution can be caused by material immobilization and aftertreatment, Ca<2+> precipitate observation can be affected because of inappropriate dyeing, and the like can be solved, and organelle can be clearly distinguished without dyeing completely.
Owner:CROP RES INST SHANDONG ACAD OF AGRI SCI
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