74 results about "Ultrastructure" patented technology

A novel cell seeded hollow fiber bioreactor is described as a potential bioartificial kidney. Endothelial cells along with pericyte, vascular smooth muscle, and / or mesangial cells or any mesenchymally derived support cells are seeded along a hollow fiber in a perfused bioreactor to reproduce the ultrafiltration function and transport function of the kidney. Maintenance of tissue specific function and ultrastructure suggest that this bioreactor provides an economical device for treating renal failure.

The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.

The invention discloses biological ink for 3D printing. The biological ink for 3D printing comprises water-soluble synthetic polymer with the cross-linking function, water-soluble natural polymer with the cross-linking function, bioactive components capable of spontaneously forming special ultrastructures, cross-linking initiator and solvents and further comprises bioactive components. The biological ink for 3D printing overcomes the defects that traditional 3d printing ink is single in component structure, does not have good biological activity and needs to utilize organic solvents, and it is difficult to take cell compatibility, biological activity and mechanical properties into consideration, the biological ink which has biological activity and the fast curing function at the same time and can form a certain microstructure is obtained, and hydrogel obtained through curing of the ink has controllable mechanical properties, good structural stability and a good fidelity effect.

A novel cell seeded hollow fiber bioreactor is described as a potential bioartificial kidney. Endothelial cells along with pericyte, vascular smooth muscle, and / or mesangial cells or any mesenchymally derived support cells are seeded along a hollow fiber in a perfused bioreactor to reproduce the ultrafiltration function and transport function of the kidney. Maintenance of tissue specific function and ultrastructure suggest that this bioreactor provides an economical device for treating renal failure.

A method for the evaluation of the ultrastructure of connective tissue, such as cartilage, including (a) providing a probe operative in the near-infrared or mid-infrared region of the electromagnetic spectrum, (b) positioning the probe either to be in contact with the connective tissue (for detecting attenuated total reflectance) or within a sufficient distance from the surface of the connective tissue (for detecting reflection), (c) detecting infrared radiation which penetrates the surface of the connective tissue for detecting attenuated total reflectance or which reflects off the surface of the connective tissue and (d) analyzing the infrared radiation from step (c) for at least one of peak height, peak area and frequency, and comparing at least one of the peak height, the peak area and the frequency for established values for at least one of peak height, peak area and frequency for normal connective tissue to detect a modification in the molecular structure of the connective tissue.

A silk protein membrane is described which is loaded with an antimicrobial compound and has a substantially non-granular ultrastructure which is (i) substantially devoid of micellar silk fibroin substructures and (ii) substantially devoid of pores when analysed by scanning electron microscopy at 0.2 μm resolution. The antimicrobial compound comprises, in one aspect of the invention, a host defense peptide. The silk protein membrane of the invention can be used in a method for the treatment of wounds and allows the wound dressing to be kept in place after removal of that wound dressing from a wound the wound has less than 105 colony forming units per gram. A method for manufacturing a wound dressing is also disclosed which comprises transferring a cast precursor material and optionally a host defense peptide, into a solid support and then drying the precursor material on the solid support to form a silk protein membrane for use as the wound dressing.

A human corneal limbal stem cell-amnion tissue engineering complex using human corneal limbal fibroblasts as the trophoblast is characterized in that: it is close to the human corneal epithelium form in histology form, and is analogous to the human corneal epithelium in immunology phenotype, its ultrastructure shows that the connection between the cells grows mature and the connection between the epithelia and the amnion carrier is a close conglutination. The culture method includes the following steps: (1) preparing the amnion for culturing the human corneal limbal stem cell in vitro; (2) preparing the human corneal limbal fibroblast trophoblast; (3) preparing the human corneal epithelia suspension; (4) culturing the human corneal limbal stem cell-amnion complex. The human corneal limbal stem cell-amnion complex has analogous phenotype to the human corneal epithelium, and provides a safe and effective biology tissue engineering product to treat corneal limbal stem cell defective eye diseases.

A method for detecting the presence of a gene responsible for a pathological state; or the pathological state itself in a patient comprising exposing at least one hair from the patient to fibre X-ray diffraction and detecting changes in the ultrastructure of the hair. An instrument (1) for detecting the presence of a gene responsible for a pathological state; or de pathological state itself, using a hair sample, comprising: an X-ray source (2) producing a beam of X-ray radiation; a sample stage for positioning said hair (3) sample within said beam; a detector to detect the scanering of said X-ray beam caused by said hair sample; and a display means (9) associated with said detector for displaying the output thereof (9a, 9b, 9c and 9d); whereby patterns of output related to the presence of said gene or said pathological state are displayed for interpretation.

The invention relates to a preparation method of TEM (transmission electron microscope) ultrathin section samples of phosphorus-accumulating bacteria, which comprises the following steps: after a sample is taken, carrying out centrifugal cleaning on the sample, putting the phosphorus-accumulating bacteria PAOs sample into a glutaraldehyde solution with a pH value being 7.4 and a volume percentage concentration of 2.5% to fix; dyeing the phosphorus-accumulating bacteria sample by using a Pb(NO3)2 solution with a mass percentage concentration of 2-10%; carrying out dehydration by using a gradient ethanol-acetone series dehydration method; and carrying out resin impregnation and embedding, and sectioning finally. The method disclosed by the invention can be used for solving the problem that, the sample pretreatment process is complicated, the toxicity of used medicaments is strong, and the ultrastructure in a PAOs cell is difficult to clearly display in existing methods; and once the preparation method is adopted for preparing PAOs samples, in TEM observation, the cell structures of bacteria, especially poly-Ps in bacteria, can be clearly and completely revealed.

The invention relates to a noninvasive sperm form and ultrastructure analysis method. The method comprises the following steps: analyzing by using a dispersion type confocal Raman spectrometer, selecting an excitation Raman spectrum, placing sperm cells under a reflected light microscopy, and acquiring the chemical group spectrum of the sperm cells through a certain excitation power and a certain exposure time; respectively dividing the acrosome, the neck and the tail of a sperm into scanning areas, scanning three times to obtain a Raman data spectrum peak, and analyzing the waveform change and the displacement of the spectrum peak; and retrieving the acquired sperm form Raman spectrums in an RRUFF Raman database to obtain the Raman spectra of all areas of the sperm form. The laser Raman spectrum adopted in the invention is a noninvasive technology, so the characteristics of the ultrastructures of different individuals and the scattering substances of different components of each sperm can be reflected without any dye or fixation, and a new assessment method is provided for the sperm morphology analysis.

The invention discloses an ecological Australia murray cod ichthyophthiriusmultifiliis preventing technology. When the ichthyophthiriusmultifiliis occurs on Australia murray cod, sea water is added into a water body where the Australia murray cod is located, detecting is conducted timely through a salinity meter, the salinity is controlled to be 8 thousandths to 10 thousandths, soaking is conducted for 48h, and the ichthyophthiriusmultifiliis is killed. After conducting some deep and basic research on the ultrastructure, the infection mechanism, pathogenesis and immunoreaction and the like of the ichthyophthiriusmultifiliis, the fact that a life cycle of the ichthyophthiriusmultifiliis is divided into stages of trophozoite, cyst and predation is found out. An ichthyophthiriusmultifiliis body is weak during the predation stage and can be restrained effectively by changing the environment, bad effects of pesticide on fish can be avoided, and meanwhile the ichthyophthiriusmultifiliis can be killed thoroughly and effectively.

The invention discloses a transmission electron microscope sample preparation method of microminiature insect compound eyes. The method includes the steps of S1, cleaning an insect body; S2, sampling;S3, fixing and rinsing; S4, fixing and rinsing again; S5, dewatering and replacing; S6, performing permeation sample soaking; S7, performing polymerization drying; S8, positioning, slicing, and drying slices; S9, dyeing and observing. The transmission electron microscope sample preparation method has the advantages that the method is based on an existing sample preparation method, and operation and treatment manners are improved according to microminiature insect tissue features; transmission electron microscope observation shows that a sample prepared by the method is clear and complete in ommatidium ultrastructure and has no evident defects, content loss, deformation and the like, so that the method is applicable to the transmission electron microscope sample preparation of the microminiature insect compound eyes and especially applicable to the transmission electron microscope sample preparation of thrips compound eyes.

A method for producing an implant from intestinal tissue 101, the intestinal tissue comprises a tubular segment 103 of intestine with at least part of its associated vasculature 102 intact, the method comprises: perfusing the vasculature 102 through a vessel thereof with at least one decellularising medium, and separately perfusing the tubular segment 103 of intestine through its lumen with at least one decellularising medium. The tubular segment 103 of intestine may be harvested from a human or non-human mammal such that at least part of the associated vasculature 102 is retained intact. Also claimed is a substantially decellularised implant derived from intestinal tissue 101 comprises a tubular component 103 and a vascular component 102, wherein a luminal surface of the vascular component 102 comprises the internal elastic lamella which displays original elastin fibre architecture and molecular ultrastructure of the vasculature from which it is derived. The apparatus for processing the intestinal implant 101 comprises a first perfusion circuit for perfusing the vasculature 102 with at least one decellularising medium, and a second perfusion circuit for separately perfusing the tubular segment 103 of intestine through its lumen with at least one decellularising medium.

The invention discloses a construction method of an in-vitro model for simulating mesenchymal stem cell transplantation, which comprises the following steps of: (1) selecting in-vitro neonatal rat cortical neurons; (2) selecting in-vitro rat mesenchymal stem cells; (3) pre-processing the in-vitro rat mesenchymal stem cells, and inoculating in the neonatal rat cortical neurons for co-culture; and (4) after the co-culture, treating the rat mesenchymal stem cells and the neonatal rat cortical neurons by a light microscope, a fluorescence microscope, an atomic force microscope and a scanning electron microscope; and determining an in-vitro model for simulating mesenchymal stem cell transplantation and a growth tendency, an interaction and formation of a synapsis-like structure between the cortical neurons and the mesenchymal stem cells on an ultrastructure. The construction method disclosed by the invention provides a simplified in-vitro model for the mesenchymal stem cell transplantation; meanwhile, the synapsis-like structure formed in the model provides an in-vitro evidence of a structure integration mechanism for the mesenchymal stem cell injury mechanism.

The invention belongs to the biomedical high molecular material technical field, in particular to a preparation method and the application of dental composite resin which contains functional monomer PMDM and modified hydroxyapatite. The preparation method includes the steps: 5%-20% of functional monomer PMDM is added to the substrate resin and placed in a constant-temperature oven; under the temperature of 40 DEG C -80 DEG C, the PMDM is dissolved in the substrate resin and stirred evenly; then photosensitizer and organic amine activator are added to the substrate resin and stirred evenly; then modified inorganic filler is added and diluted in solvent; the amount of solvent is 5%-10% of the mass of the mixture; the mass fraction of inorganic filler is 20%-70% of the total mass; inorganic filler is stirred evenly; the mixture is placed in a vacuum oven and placed under 40 DEG C-80 DEG C for 2-4 hours; the composite resin paste is obtained after the solvent is volatilized completely and the air bubbles are eliminated. The oral cavity repair resin has the advantages of good bond performance, ultrastructure, higher mechanical intensity, higher biocompatibility, having wide application prospect in the oral cavity repair field, having reasonable preparation line, obtainable raw materials, lower price, simple preparation process, having important popularization and application values.

The invention provides a quick and simple leafhopper digestive organ anatomy method and belongs to the field of entomotomy. According to the method, a complete leafhopper digestive tract can be obtained in a short time, losses of components of cells can be reduced to the greatest degree, it can be guaranteed that initial conditions of ultrastructures in the cells can be kept complete, and good results in the next step of experiment can be obtained conveniently. According to the method, the digestive tract is connected with salivary glands of the head and the rectum of the tail, the digestive tract can be dissected out carefully through a drawing and pulling method, digestive organs are not damaged, leafhopper shells are not destroyed, and the completeness of insects are reserved to the greatest degree.

The invention provides an application of a pharmaceutical composition in the preparation of drugs for improving cerebral microcirculation dysfunction. The application uses an ischemia-reperfusion model which is established by the ligation of bilateral carotid arteries of a Mongolian gerbil, the dynamic cerebral microcirculation including cerebral blood flow, blood velocity, blood vessel diameter, generation of vascular endothelial peroxides, leukocyte adhesion and change of vascular permeability of the Mongolian gerbil is observed through a visual dynamic continuous system after the ligation of the bilateral carotid arteries, and a projecting and scanning electron microscope is further used for observing the changes of ultrastructures of small veins and capillaries for observation and research, thereby proving that Yangxueqingnao particles have the effects of treating and improving the cerebral microcirculation dysfunction caused by ischemia-reperfusion.

The invention discloses a traditional Chinese medicine preparation for assisting acupuncture treatment of diabetic gastroparesis and a preparation method and applications thereof. The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 8-10 parts of astragalus membranaceus, 8-10 parts of codonopsis pilosula, 8-10 parts of atractylodes macrocephala koidz, 3-6 parts of fructus amomi, 6-8 parts of hovenia dulcis, 6-8 parts of salvia miltiorrhiza and 6-8 parts of endothelium corneum gigeriae galli. The traditional Chinese medicine preparation is prepared by decocting raw herbal materials with water, then extracting and concentrating. Pharmacodynamic experiments show that the traditional Chinese medicine preparation for assisting acupuncture treatment of diabetic gastroparesis has no obvious adverse reaction, and is safe and reliable; the preparation can be used for promoting the increase of body mass, food intake and stool quantity of mice with diabetic gastroparesis, relieving the gastric electrical activity confusion and improving the gastric antrum ICC ultrastructure very well, so that the expression of positive ICC and SP positive materials of gastric antrum c-kit can be enhanced, and the positive expression of nNOS can be lowered; and the preparation has a good improvement effect on the number increasing, form and signal transduction function of ICC.

The invention provides a method for making a paraffin section of the elytrum of an insect. The method comprises the following steps: 1, distinguishing female and male coleoptera insects in a pupal stage and taking the elytra of the insects after eclosion for preparation of paraffin sections; 2, fixing the elytra, i.e., cutting the to-be-observed parts of the elytra obtained in the step 1 and fixing the parts with an FAA fixing agent; 3, dehydrating the elytra, i.e., successively dehydrating the fixed elytrum tissue with alcohols with different volume concentrations; 4, transparentizing the elytra, i.e., transparentizing the dehydrated elytra in a solution of anhydrous alcohol and xylene at first and then in xylene; 5, impregnating the elytra with paraffin, i.e., putting the transparentized elytra in a solution of soft paraffin and xylene with a temperature of 55 DEG C for 3 h at first and then in a solution of hard paraffin and xylene with a temperature of 65 DEG C for 3 h, and then carrying out excessive paraffin impregnation at a constant temperature of 65 DEG C for 6 h; 6, carrying out embedding and slicing; 7, carrying out unfolding, pasting and roasting; 8, carrying out paraffin removal and rehydration; and 9, carrying out dyeing and mounting. The elytrum paraffin section prepared by using the method has a high integrity rate and can meet requirements of research on the microstructure and ultrastructure of the elytra.

The invention discloses an organ chip. The organ chip comprises a No.1 outer-layer chip, a No.1 porous layer is arranged on the outer surface of the lower end of the No.1 outer-layer chip, a middle-layer chip is arranged on the outer surface of the lower end of the No.1 porous layer, and a middle-layer chip is arranged on the outer surface of the lower end of the middle-layer chip. By the aid of the middle-layer chip, the organ chip can simulate tiny structures in human liver organs by a bionic cell culture chamber, a bionic left rear superior border vein, a bionic middle hepatic vein, a bionic left medial lobe vein branch, a bionic left outer lobe vein branch, a bionic caudate lobe vein, a bionic right rear superior border vein, a bionic right hepatic vein, a bionic auxiliary middle hepatic vein, a bionic right anterior lobe vein branch and a bionic right rear upper vein, ensure that cells can better adapt to simulation survival environment, form ultrastructures of liver organs in human bodies and more accurately simulate cell survival environments in human bodies so as to improve medicine detection accuracy and efficiency.

The invention discloses a method for sequencing partial DNA of a mitochondrial Cytb gene of a brachionus rotifer and a method for identifying the brachionus rotifer, which comprises the following steps: extraction of DNA from the brachionus rotifer; PCR primer amplification and sequencing, wherein the PCR primer is Cytb1S: 5'-TWGATYTWCCTTCTCCHGC-3'; Cytb1R:5'-GGWGAYGCWGGACAD CTHCC-3; system analysis. The PCR primers can amplify part of the DNA of the mitochondrial Cytb gene, and the results can be used for precise identification of the rotifer, the identification problem caused by using morphology and ultrastructure is solved. In the process of large-scale culture of the rotifers, the competition of rotifers caused by mixed populations, which causes output reduction and large-scale death of the rotifers, can be reduced.

The invention provides a method of detecting neoplastic or neurological disorders comprising exposing skin or nails to X-ray diffraction and detecting changes in the ultrastructure of the skin or nails, and also provides an instrument when used in the method of detection.

The invention discloses a preparation method of a decellularized nerve scaffold and an effect evaluation method of the preparation method. According to the preparation method, the decellularized nervescaffold is prepared by adopting a mode of combining TritonX-100 and a freeze-thaw enzyme. The decellularized effect on the decellularized nerve scaffold is evaluated through ways of morphological observation, ultrastructure observation, cytotoxicity detection, biocompatibility detection, complex frozen section observation and the like. The result shows that the decellularized nerve scaffold which is prepared by adopting a mode of combining the TritonX-100 and the freeze-thaw enzyme has the advantages of sufficient decellularized effect, complete three-dimensional space structure, smooth canals, good compatibility with cells and tissues, slight inflammatory reaction and good cell growth and proliferation state, is suitable for adhesion growth of cells, and is an ideal scaffold material.

The invention discloses a method for preparing a xylem sample of haloxylon ammodendron for a scanning electronic microscope. The method is characterized by mainly comprising the steps of sample preparation, sample dehydration, sample drying, sample corrosion, bonding the sample to a table and coating the sample with a film. Compared with the prior art, the sample is complete in structure, the organization structure in the tangent plane of the xylem is clear when the sample is observed by the scanning electronic microscope, images are highly stereoscopic, and the ultrastructure of the xylem of the haloxylon ammodendron can be conveniently observed and analyzed.

The invention relates to a special nano zirconium dioxide composite powder material for glass reflective insulation paint, belonging to the field of manufacturing of metal oxide ultrastructures. The material is characterized by comprising the following chemical components in percentage by mass: 70-90% of zirconium dioxide (ZrO2) and 10-30% of titanium dioxide (TiO2). The preparation method comprises the following steps: continuously spraying an atomized ammonium bicarbonate water solution into an anhydrous ethanol solution (which is prepared by respectively preparing zirconium oxychloride (ZrOCl2.8H2O) and tetrabutyl titanate and mixing evenly) in a stirring state to react to obtain a mixed sol, carrying out azeotropic dehydration on the mixed sol by using butanol as an azeotrope former to obtain a dry material, and calcining to obtain the special nano zirconium dioxide composite powder material for glass reflective insulation paint, of which the particle size is 30-50nm and the specific area is greater than or equal to 30 m<2> / g. The invention provides a high-activity high-dispersity high-stability special nano zirconium dioxide composite powder material for glass reflective insulation paint, and a preparation method and application method thereof. The sunlight reflectance of the prepared paint for white is up to 0.85; and the hemispherical emittance is up to 0.85.

The invention provides an application of an acanthopanax extract in preparing medicine for preventing or treating cardiac toxicity, and belongs to the field of medicine. The inventor finds that the acanthopanacis senticosi extract can effectively prevent or treat cardiac toxicity caused by adriamycin, can increase myocardial function, improve cardiac configuration and cardiac remodeling, alleviatemyocardial tissue ultrastructure and pathological change, and inhibit apoptosis of damaged myocardial cells and reduce oxidative and inflammatory damage of myocardial cells. The extract can be used for preparing medicine for preventing or treating cardiac toxicity, thereby protecting myocardial cells, avoiding side effects of adriamycin and having wide application prospect.

The invention discloses an ultrastructure lens and a manufacturing method thereof. The method comprises the steps that the focus of the ultrastructure lens is determined; a design variable of the surface type of the ultrastructure lens is set in a cross section layer of a cross section assembly, wherein the cross section assembly comprises a substrate, the cross section layer and a perfect matching layer, and excitation waves are distributed inside the cross section assembly; exponential power law mixed material interpolation of a pre-selected material and a vacuum relative dielectric constantin the cross section layer is carried out according to the design variable, and the distribution position of the pre-selected material is determined to obtain the cross section structure configuration of the ultrastructure lens so as to maximize the electric field energy density at the focus, wherein the pre-selected material is the material of the ultrastructure lens, and the cross section structure configuration is rotated along the symmetry axis of the ultrastructure lens to obtain the structure configuration of the ultrastructure lens; and according to the structural configuration, the ultrastructure lens is machined and obtained. The structure configuration is reversely designed according to the required ultrastructure lens, the ultrastructure lens is machined and obtained, the convenience is achieved, the geometric structure data size of the ultrastructure lens is reduced by obtaining the profile structure configuration, and the manufacturing efficiency is improved.

The invention discloses a method for building a lipopolysaccharide induced piglet liver cell programmed necrosis model. The method includes: performing lipopolysaccharide induction on a test piglet toobtain a to-be-measured piglet; sampling blood from the precaval vein of the to-be-measured piglet, separating serum, measuring biochemical indexes in the serum, and judging the influence of the lipopolysaccharide induction on the liver function of the piglet; taking the liver sample of the to-be-measured piglet, observing the shape structure of liver tissue and the ultrastructure of liver cells,measuring the key genes of liver cell programmed necrosis and the protein expression of the key genes, and analyzing the effect of the lipopolysaccharide induction on the piglet liver cell programmednecrosis. The method has the advantages that lipopolysaccharides are used as the induction agent, the liver cells are used as the model cells of the cell programmed necrosis model, and the piglet isused as the modeling object to successfully build the liver cell programmed necrosis model, and the method is simple and practical to operate; by measuring the key genes of the liver cell programmed necrosis and the protein expression of the key genes, the method is reliable in measuring result and good in repeatability.

The invention provides a medicine for treating primary osteoporosis through a kidney-tonifying, spleen-strengthening and blood-stasis-removing method. The medicine is prepared from epimedium, antler gum, tortoise-plastron glue, semen cuscutae, cinnamon, rhizoma cibotii, achyranthes roots, eucommia bark, turtle shells, drynaria rhizome, astragalus, teasel roots, dried rehmannia roots, cornus officinalis, Chinese yam, poria cocos, moutan bark, rhizoma alismatis, radix angelica sinensis, white peony roots, radix salvia, leeches, dicranopteris linearis, aster striatus, orixa japonica and raw licorice roots. The medicine can improve the shape of bone trabecula by inhibiting bone loss caused by osteoclast, effectively improves ultrastructures of osteoporotic cancellous bones, increases bone mass, prevents and repairs microscopic fracture, and achieves the curative effect of preventing osteoporosis.