Noninvasive sperm form and ultrastructure analysis method
An analysis method and ultrastructure technology, applied in the direction of material excitation analysis, Raman scattering, etc.
Inactive Publication Date: 2014-04-02
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
View PDF0 Cites 11 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
At present, there is no report on the establishment of a single sperm morphology analysis method using a laser Raman spectroscopy system
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0025] 1. Research materials and methods
[0026] 1. Experimental equipment
[0027] 1.1 Laser Raman Spectroscopy System
[0028] Dispersive confocal Raman spectrometer (Senterra R200-L), produced by Bruker, Germany, with spectral resolution ≤ 1.5cm -1 , horizontal resolution < 1um, vertical resolution < 2 um, equipped with three lasers with wavelengths of 785nm, 633nm and 532nm, three-dimensional automatic control platform and other important performances.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more PUM
![No PUM](https://static-eureka-patsnap-com.libproxy1.nus.edu.sg/ssr/23.2.0/_nuxt/noPUMSmall.5c5f49c7.png)
Abstract
The invention relates to a noninvasive sperm form and ultrastructure analysis method. The method comprises the following steps: analyzing by using a dispersion type confocal Raman spectrometer, selecting an excitation Raman spectrum, placing sperm cells under a reflected light microscopy, and acquiring the chemical group spectrum of the sperm cells through a certain excitation power and a certain exposure time; respectively dividing the acrosome, the neck and the tail of a sperm into scanning areas, scanning three times to obtain a Raman data spectrum peak, and analyzing the waveform change and the displacement of the spectrum peak; and retrieving the acquired sperm form Raman spectrums in an RRUFF Raman database to obtain the Raman spectra of all areas of the sperm form. The laser Raman spectrum adopted in the invention is a noninvasive technology, so the characteristics of the ultrastructures of different individuals and the scattering substances of different components of each sperm can be reflected without any dye or fixation, and a new assessment method is provided for the sperm morphology analysis.
Description
technical field [0001] The invention relates to a sperm morphology and structure analysis method, in particular to a non-invasive sperm morphology and ultrastructure analysis method. Background technique [0002] At present, about 20% of couples of childbearing age in the world suffer from infertility, and male causes account for about 50% of infertility, while more than 60% of male infertility is due to oligozoospermia and asthenospermia. totally clear. At present, clinicians in many hospitals still rely on the routine semen analysis indicators in the laboratory to choose clinical treatment plans. Although the application of routine semen analysis has a long history, many medical institutions still use manual microscope analysis technology; grayscale computer-aided sperm analysis (CASA, Computer-aided sperm analysis) analysis method has been widely used clinically for many years; However, the CASA analysis method of phase contrast microscopy is still very limited in hospi...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Application Information
Patent Timeline
![application no application](https://static-eureka-patsnap-com.libproxy1.nus.edu.sg/ssr/23.2.0/_nuxt/application.06fe782c.png)
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 李铮刘锋朱勇何琳刘宇飞
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Try Eureka
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap