38 results about "Sperm morphology" patented technology

The invention discloses a sperm morphology analysis method based on a deep neural network model, and the method comprises the steps: recognizing and positioning sperms in a microscopic image through asperm image positioning module, extracting a sperm image, finally analyzing the image of each sperm through a sperm morphology analysis module, and completing the classification. According to the invention, the influence of interference factors on detection results can be greatly weakened, and the reliability of sperm morphology analysis results is improved. Feature information which cannot be obtained by a traditional measurement method in sperm pictures can be captured, so that the accuracy of sperm morphology analysis is improved. The sperm morphology analysis requirements of different sperm classification targets can be met.

The invention discloses a sperm morphology anomaly detection method which is highly objective, capable of facilitating verification and improving detection efficiency, and broad in application prospect. The method comprises the steps of (1) acquiring the image of a sperm processed with a chemical reagent; (2) preprocessing the acquired image, wherein graying, contrast enhancement, image smoothing and denoising and binarization are conducted; (3) segmenting the preprocessed image, wherein impurity filtering and target extraction are conducted on the binarized image; (4) conducting profile identification so that the accurate overall sperm target profile and head profile can be obtained; (5) obtaining all parameter values so that relevant parameters of the sperm morphology can be directly judged.

The invention belongs to the technical field of image recognition, and particularly relates to a medical sperm image recognition system based on deep learning. The system comprises an input module, apositioning module and a classification module, wherein the input module is used for collecting sperm pictures after graying processing of a detector; the positioning module is used for positioning asperm head on the sperm picture by utilizing a YOLO v3 model in a deep learning and image recognition method according to the sperm picture acquired in the input module; the classification module adopts the constructed VGG-sense block classification model to carry out positive anomaly judgment on the sperm heads positioned in the positioning module, and outputs normal sperms and abnormal sperms. The system is short in sperm picture detection time, greatly reduces the workload of doctors, is high in accuracy, reduces errors caused by subjectivity, can assist and partially replace the doctors toperform sperm morphology evaluation, and has a good application prospect.

The invention relates to a noninvasive sperm form and ultrastructure analysis method. The method comprises the following steps: analyzing by using a dispersion type confocal Raman spectrometer, selecting an excitation Raman spectrum, placing sperm cells under a reflected light microscopy, and acquiring the chemical group spectrum of the sperm cells through a certain excitation power and a certain exposure time; respectively dividing the acrosome, the neck and the tail of a sperm into scanning areas, scanning three times to obtain a Raman data spectrum peak, and analyzing the waveform change and the displacement of the spectrum peak; and retrieving the acquired sperm form Raman spectrums in an RRUFF Raman database to obtain the Raman spectra of all areas of the sperm form. The laser Raman spectrum adopted in the invention is a noninvasive technology, so the characteristics of the ultrastructures of different individuals and the scattering substances of different components of each sperm can be reflected without any dye or fixation, and a new assessment method is provided for the sperm morphology analysis.

The invention discloses a sperm morphology detection method and device based on an image technology, and relates to the computer vision field. A specific embodiment of the method comprises the following steps: identifying any one independent sperm in a to-be-detected sperm image; obtaining contours of key parts of the independent sperms; determining the central coordinates of the key parts of theindependent sperms according to the contours of the key parts of the independent sperms; based on the to-be-detected semen image and the central coordinates of the key parts of the independent sperms,extracting the contours of the independent sperms; and performing morphological detection on the independent sperms according to the contours of the independent sperms. According to the embodiment, the sperm detection efficiency is improved.

A sperm morphological fast stain consists of solution A containing Nile blue fixation liquid of tissues for staining sperm apical body region. A method for fast staining includes preparing sperm suspension film preparation, drying by air, using said solution A to cover film preparation till sperm apical body region be stained.

The invention belongs to the field of cytobiology research, and specifically belongs to the field of sperm morphology positioning research. A grid positioning sheet disclosed in the invention comprises a glass substrate, and a polished glass sheet; the glass substrate and the polished glass sheet are glued together; the polished glass sheet is provided with a square grid zone via drawing; the square grid zone comprises 25 1mmX1mm big grids arranged with intervals of 0.1mm; the top left of each big grid is marked with a capital letter selected from A to Y; each big grid comprises 100 0.1mmX0.1mm square small grids; along the side frame of each big grid from the top to the bottom and from the left to the right, the small grids are marked with numbers from 0 to 9; the numbers and grid lines on the grid positioning sheet are all displayed via highlight black lines. The sperm grid positioning sheet is suitable for observing of sperm morphology under a high-power microscope; accurate positioning of the observed sperms can be realized via the big and the small grids which are marked with English letters and Arabic numerals; and it is extremely convenient to find target sperms when repeated checking on specific sperms is necessary.

A method of analyzing a semen sample having living sperm is provided comprising receiving from a remote location a set of digital video clips of a prepared semen sample having living sperm and analyzing a subset of the digital video clips taken from the prepared semen sample using a Computer Assisted Semen Analyzer adapted to analyze the subset of digital video clips received from a remote location. A method of measuring sperm morphology is also provided comprising receiving from a remote location a digital video clip of a prepared semen sample having stained non-living sperm, and, analyzing portions of the digital video clip taken from the prepared semen sample.

The invention discloses a method for identifying and classifying sperms in a morphological image. The method comprises the following steps of: collecting morphological images of the sperms and carrying out image binarization processing on the morphological images; defining the sperms in the morphological images subjected to the image binarization processing as candidate sperms possibly belonging to real sperms, calculating characteristic values of the candidate sperms in the morphological image, providing a preset fixed threshold value, judging whether the characteristic values of the candidate sperms meet the range of the preset fixed threshold value or not, and determining whether the characteristic values of the candidate sperms meet the range of the preset fixed threshold value or not;if yes, defining the candidate sperms as real sperms; and, if not, excluding the sperms from the candidate sperms, comparing the real sperms with various sperm forms pre-stored in a neural network through the neural network, and classifying the real sperms.

The invention discloses a sperm morphology rapid dyeing reagent and a dyeing method thereof. The sperm morphology rapid dyeing reagent is prepared from a solution A, a solution B and a solution C; thesolution A is tissue fixative containing fast green; the solution B is a preservation solution containing eosin; and the solution C is phosphate buffer containing alkaline azure A and methylthioninechloride. The dyeing method comprises the steps that fresh seminal fluid is collected and liquidized in a water-bath pot with the temperature being 20-37 DEG C, sperms are optimized for 8-12 minutes by adopting a swim-up method, and the fresh sperms with high activity are obtained; sperm suspension and a smear are prepared, and the smear is dried under a sterile environment; the smear is dipped into the solution A and reacts for 15 seconds at room temperature so as to remove redundant fixative; the smear is dipped into the solution B and reacts for 10 seconds at room temperature so as to remove the redundant dyeing agent; the smear is dipped into the solution C and reacts for 10 seconds at room temperature so as to remove the redundant reagent; the redundant dyeing agent is removed; moisture is removed, and after the smear is completely dried, dyeing is completed. The sperm morphology rapid dyeing reagent well solves the defects that sperm dyeing is complex to operate, and the cost ishigh in the prior art.

The invention provides a multifunctional sperm quality analysis system. The multifunctional sperm quality analysis system comprises sperm routine computer-aided sperm analysis (CASA) and image analysis, the CASA is used for analyzing a sample video recorded through a CCD camera or video inputted into the local of an analysis host to calculate the quantity, the concentration, the vitality and the motion trail of sperms of the sample; the image analysis is used for analyzing sperm functions and comprises sperm DNA fragment detection, sperm nucleoprotein detection and sperm morphological detection, and pictures taken through the CCD camera or pictures inputted to the local of the analysis host are analyzed. The problems that existing seminal fluid analysis operation is tedious, human factorsare uncontrollable, and the inspection cost is high are solved.

The invention provides a novel application of plant fruit pulp. The invention relates to application of plant fruit pulp in testis function protection, application of plant fruit pulp in prevention and / or treatment of oligospermia and asthenospermia, application of plant fruit pulp in protection of male fertility and application of plant fruit pulp in prevention and / or treatment of testis tissue lesion, testosterone level decline, testis function decline, sperm number reduction, asthenospermia and asthenospermia caused by metabolic disorder. The sperm motility rate is reduced; the sperm morphology is abnormal. The plant fruit pulp can effectively prevent and treat symptoms such as testicular tissue lesion, testosterone level decline, testicular hypofunction, sperm number reduction, sperm motility rate reduction and sperm morphology abnormality caused by metabolic disorder. The application provides a basis for protecting testis functions, preventing and / or treating oligospermia and asthenospermia and protecting male fertility by directly utilizing the plant fruit pulp.

Systems and methods are provided for provided for automatic evaluation of sperm morphology. An image of a semen sample is obtained, and at least a portion of the image is provided to a convolutional neural network classifier. The convolutional neural network classifier evaluates the portion of the image to assign to the portion of the image a set of likelihoods that the portion of the image belongs to a plurality of output classes representing the morphology of sperm within the portion of the image. A metric is assigned to the semen sample based on the likelihoods assigned by the convolutional neural network.

The invention provides a marking method for an intelligent sperm morphology reading system. The marking method comprises the following steps of S1) carrying out HSV image conversion processing on a dyed slide image; S2) carrying out image binarization processing; S3) constructing a structural element of a 5*5 unit matrix, performing an operation of first expanding and then corroding the image, andextracting an image contour; S4) judging all the contours, and deleting the contours with the number of contained points smaller than a threshold value; and S5) excluding all contours which do not meet the requirements, updating the contour list, and finishing the drawing of the contour marks. The method has the advantages that a man-machine interaction solution of a whole set of radiograph reading process from sperm image generation to single sperm labeling, radiograph reading conclusion generation and multi-person cross radiograph reading is provided; the method aims to improve the intelligence and unmanned performance of medical film reading, and can be applied to various working environments needing to mark a large number of targets, such as the fields of bone detection, CT films andthe like.

The invention relates to a sperm quality detection method and device, computer equipment and a storage medium. The method comprises the following steps: acquiring a plurality of to-be-detected sperm images; detecting the to-be-detected sperm image to obtain a sperm morphology detection result of sperms in the to-be-detected sperm image; detecting each to-be-detected sperm image to obtain a sperm number detection result and a sperm position detection result corresponding to each to-be-detected sperm image; determining the sperm concentration according to the sperm number detection result of the plurality of to-be-detected sperm images; and generating a sperm motility detection result according to the sperm position detection results of the plurality of to-be-detected sperm images. By adopting the method, the sperm morphology detection result and the conventional sperm detection result can be quickly obtained, so that the working process of sperm quality detection is greatly simplified, and the sperm quality detection efficiency is improved.

The invention belongs to the technical field of sperm morphology detection, and particularly relates to an automatic sperm morphology analysis method based on multi-scale feature fusion, which comprises the following steps: scanning a sperm sample image through a microscope automatic scanning platform, performing normalization processing on the obtained sperm sample image, extracting features of sperms on multiple scales by using an SD Net network model, fusing the features, outputting coordinates and bounding box information of the sperms on the sperm sample image, and detecting sperm images existing in the sperm sample image; and carrying out feature extraction and regional feature fusion on the detected sperm image by using an SR Net network model, and finally completing the recognition of abnormal sperm cells. Compared with a general deep learning method, the invention has the advantages that by fusing the features on multiple scales, the method has better detection capability on sperms with smaller sizes, the detection accuracy is higher, the generalization is stronger, and a very good detection effect can also be obtained under the condition of larger sample image difference.

The invention relates to a method for simultaneously detecting completeness of a sperm nucleus protein and a membrane, which comprises the following steps: (1) preparing a hypotonic solution; (2) preparing a sperm morphology fixing solution; (3) carrying out simple sperm hypotension and morphology fixing treatment; (4) centrifuging the semen with fixed sperm morphology, standing to stratify, discarding the supernatant, and diluting the obtained semen with a phosphate buffer solution for sperm sediment to obtain a diluted semen; (5) sufficiently and evenly mixing the diluted semen and phosphate buffer solution, centrifugating, discarding the supernatant, washing the sperm precipitate with the phosphate buffer solution, and adding gelatin to the washed sperm precipitate to prepare a semen; (6) putting the prepared semen on a glass slide, and drying; (7) fixing the glass slide at 25 DEG C by adding the sperm morphology fixing solution; (8) adding aniline blue into the glass slide for dyeing; (9) flushing the glass slide with tap water, and immersing the glass slide in 0.9 wt% sodium chloride for decolorization; and (10) naturally airing or blow-drying the glass slide, adding 2 drops of glycerol sealing solution, and sealing with a cover slip.

The invention discloses application of a Shorr staining reagent and a staining method, and relates to the technical field of assisted reproduction. The invention discloses application of a Shorr staining reagent in preparation of a sperm morphology diagnostic kit. A sample of the diagnostic kit is a testis needle biopsy tissue frozen section. According to the kit, fresh testis needle biopsy tissue frozen sections serve as staining objects, all parts of sperms can be stained in different colors, the sperms and background cells can be effectively distinguished, the recognition degree of the sperms is improved, and the diagnosis time of the pathological morphology of the sperms is shortened.

The invention relates to a method for preparing a sperm slide specimen of Neoseiulus californicus. The method is characterized by being a method of preparing the sperm slide specimen after obtaining a spermatophore from the gnathosoma of a male Neoseiulus californicus which is in mating; the method comprises five parts of obtaining of a female Neoseiulus californicus and the male Neoseiulus californicus which are in mating, shaping of the female Neoseiulus californicus and the male Neoseiulus californicus, separating of the female Neoseiulus californicus and the male Neoseiulus californicus, obtaining of the spermatophore, obtaining of sperm and preparation of the slide specimen. According to the method provided by the invention, the spermatophore of the male Neoseiulus californicus can be obtained by a very simple and ingenious method, and the sperm slide specimen is prepared; the obtaining of the spermatophore can provide a raw material for the preparation and research of the sperm slide specimen of the Neoseiulus californicus, and the preparation of the sperm slide specimen can provide reference for understanding the sperm morphology of the Neoseiulus californicus and revealing the reproduction principle of the Neoseiulus californicus.

The invention provides a novel application of plant seed oil. The invention relates to application of plant seed oil in testis function protection, application of plant seed oil in prevention and / or treatment of oligospermia and asthenospermia, application of plant seed oil in protection of male fertility and application of plant seed oil in prevention and / or treatment of testis tissue lesion, testosterone level decline, testis function decline, sperm number reduction, and asthenospermia caused by metabolic disorder. The sperm motility rate is reduced; the sperm morphology is abnormal. The plant seed oil can effectively prevent and treat symptoms such as testicular tissue lesion, testosterone level decline, testicular hypofunction, sperm number reduction, sperm motility rate reduction and sperm morphology abnormality caused by metabolic disorder. The application provides a basis for protecting testis functions, preventing and / or treating oligospermia and asthenospermia and protecting male fertility by directly utilizing the plant seed oil.

The invention relates to the technical field of medical apparatus and instruments, in particular to an automatic sperm morphological detection slide making device and method. The automatic sperm morphology detection slide making device comprises a control system, a sample loading device, a dyeing device, a flushing device and a blow-drying device. The sample loading device consists of a sample loading groove; the dyeing device is composed of dyeing liquid pipes (including a first dyeing liquid pipe and a second dyeing liquid pipe), a liquid inlet and a liquid outlet, the flushing device is composed of a spray head and a flushing pipeline, and the blow-drying device is composed of an air dryer (including a front baffle, a rear baffle, fan blades, a motor and an air bellow). The invention further comprises a button and a power supply. According to the invention, automation of sperm morphological detection slide making is realized, the operation is simple, the carrying is convenient, flaking precision can be realized while flaking is rapid, and the practicability is very high.

The invention relates to a method for inducing skin stem cells to differentiate into sperms. The method comprises the following steps of firstly, co-culturing the skin stem cells of a mouse and fibroblast treated by mitomycin C; inducing to differentiate into PGCs (primordial germ cells); then, reversely injecting PGC cell mixing liquid into a testis seminiferous tubule through an efferent ductule of a recipient mouse; after developing for one month in the testis of the recipient mouse, fetching out the testis of the recipient mouse; after HE staining analysis, finding the recovery of spermatogenesis in the seminiferous tubule, emerging of cells with typical sperm morphology, and expression of GFP and MVH of spermatogenic cells in the seminiferous tubule with recovery of spermatogenesis. The method has the advantages that the injury to a body is little, the limitation of ethical issue is avoided, the skin stem cells are effectively induced to be differentiated into sperm cells, the differentiation efficiency is high, the sperm cells obtained by differentiation have typical sperm morphology, and the specific gene of the sperm cell is expressed.

The invention relates to a full-automatic computer-assisted seminal fluid quality analysis system. The system consists of seven parts, namely a sample management part, a consumable and reagent management part, a test item management part, a sperm image analysis part, a report management part, a user management system and a system setup part, and the sperm image analysis part comprises a full-automatic seminal fluid sample pretreatment module and a full-automatic sperm quality analysis module. According to the invention, an instrument can be controlled to replace manual operation and manual interpretation, various indexes of the semen sample can be detected in a full-automatic manner, and the full-automatic semen sample pretreatment module provides guarantee for the accuracy rate of subsequent sperm morphology detection based on deep learning.

The invention discloses a counting slide assembly and a sperm morphological activity concentration detection system, and belongs to the technical field of medical equipment. A glass slide of the counting glass slide assembly is divided into a counting observation area and an information identification area, the counting observation area is provided with at least two rectangular grooves used for preparing samples, and the samples can be sperm samples which are only liquefied at normal temperature. An operator can compare sperm samples under different treatment degrees on one glass slide at the same time, so that the detection precision is improved; the depth of each rectangular groove is not greater than 10 microns, so that a single-layer sperm sample can be formed, and the single-layer sperm sample is analyzed to obtain the corresponding morphological concentration activity of the single-layer sperm sample; an operator can record the detection serial number of the sperm in a marking area, so that the detection serial number can be checked at any time in the later period; the two-dimensional code is adopted for recording information of a patient providing sperms, and personal privacy is protected; and the cover glass only covers the counting observation area, so that the length of the cover glass is shortened, and the production cost is reduced.

The invention provides a compound traditional Chinese medicine for treating masculine sterility, which comprises the following components in parts by mass: 8-15 parts of polyporus umbellatus, 6-12 parts of tuckahoe, 6-12 parts of oriental waterplantain rhizome, 6-12 parts of talcum, 10-30 parts of donkey-hide gelatin, 10-30 parts of rehmannia glutinosa, 8-15 parts of China dodder, 8-15 parts of raspberry and 4-6 parts of leech. The compound traditional Chinese medicine disclosed by the invention is mainly used for treating sterility due to male dysspermatism; after being verified by a large amount of clinical tests, the compound traditional Chinese medicine can be used for quickly recovering various objective indicators of dysspermatism patients, such as sperm count, sperm morphology, sperm pH value, sperm viscosity, sperm liquefying time, sperm motility, sperm viability rate and the like to be normal within one to three weeks, thereby the successful conception is achieved. Meanwhile, the compound traditional Chinese medicine has definite curative effect for sterility caused by sperm immune abnormality. By administrating the compound traditional Chinese medicine, the quality of semen can be improved as soon as possible, thereby the semen is ensured to be normal and impregnated.

The embodiment of the invention discloses a sperm morphology recognition method based on a deep convolutional neural network and a storage medium. The method comprises the following steps: acquiring a multi-sperm image, and performing image segmentation processing on the multi-sperm image to obtain at least one single sperm image; extracting at least one sperm part in each single sperm image, and determining a part contour image of each sperm part, wherein the sperm part comprises at least one of a head part, an apical body, a cavitation bubble, a neck part and a tail part; and recognizing the part contour image based on a deep convolutional neural network to obtain a sperm morphology recognition result of the single sperm, wherein the sperm morphology recognition result comprises normal sperm morphology or abnormal sperm morphology. According to the technical scheme disclosed by the embodiment of the invention, the sperm morphology recognition is more accurate and rapid, and the efficiency and quality of sperm morphology analysis are improved.

A semi-automatic sperm staining instrument, comprising a rectangular base, several staining solution containers arranged side by side along the long sides at intervals on the base, and a padding device arranged above the base, the padding device includes a long side and a base A pressing plate with a horizontal short side, several sperm-coated glass sheets arranged on the pressing plate, sliding connection devices hinged at both ends of the lower long side of the pressing plate, so that the pressing plate can be turned up and down and reciprocally slide along the long side of the base. The pressure dyeing method and the use of special instruments to control the time also solve the problem of easy mixing of dyes. At the same time, there are washing and air-drying devices, and the standardization of sperm staining can be achieved at a fixed room temperature. It solved the problem of standardization of sperm morphology staining and laid the foundation for the standardization of sperm morphology diagnosis.

A sperm morphological fast stain consists of solution A containing Nile blue fixation liquid of tissues for staining sperm apical body region. A method for fast staining includes preparing sperm suspension film preparation, drying by air, using said solution A to cover film preparation till sperm apical body region be stained.

The invention provides a compound traditional Chinese medicine for treating masculine sterility, which comprises the following components in parts by mass: 8-15 parts of polyporus umbellatus, 6-12 parts of tuckahoe, 6-12 parts of oriental waterplantain rhizome, 6-12 parts of talcum, 10-30 parts of donkey-hide gelatin, 10-30 parts of rehmannia glutinosa, 8-15 parts of China dodder, 8-15 parts of raspberry and 4-6 parts of leech. The compound traditional Chinese medicine disclosed by the invention is mainly used for treating sterility due to male dysspermatism; after being verified by a large amount of clinical tests, the compound traditional Chinese medicine can be used for quickly recovering various objective indicators of dysspermatism patients, such as sperm count, sperm morphology, sperm pH value, sperm viscosity, sperm liquefying time, sperm motility, sperm viability rate and the like to be normal within one to three weeks, thereby the successful conception is achieved. Meanwhile, the compound traditional Chinese medicine has definite curative effect for sterility caused by sperm immune abnormality. By administrating the compound traditional Chinese medicine, the quality of semen can be improved as soon as possible, thereby the semen is ensured to be normal and impregnated.

A method of automated measurement of motility and morphology parameters of the same single motile sperm. Automated motility and morphology measurements of the same single sperm are performed under different microscope magnifications. The same single motile sperm is automatically positioned and kept inside microscope field of view and in focus after magnification switch. A method of automated non-invasive measurement of sperm morphology parameters under high magnification of imaging. Sperm morphology parameters including subcellular structures are automatically measured without invasive samplestaining. A method of automatically selecting sperms with normal motility and morphology and DNA integrity for infertility treatment.