Method for preparing sperm slide specimen of Neoseiulus californicus

Abstract

The invention relates to a method for preparing a sperm slide specimen of Neoseiulus californicus. The method is characterized by being a method of preparing the sperm slide specimen after obtaining a spermatophore from the gnathosoma of a male Neoseiulus californicus which is in mating; the method comprises five parts of obtaining of a female Neoseiulus californicus and the male Neoseiulus californicus which are in mating, shaping of the female Neoseiulus californicus and the male Neoseiulus californicus, separating of the female Neoseiulus californicus and the male Neoseiulus californicus, obtaining of the spermatophore, obtaining of sperm and preparation of the slide specimen. According to the method provided by the invention, the spermatophore of the male Neoseiulus californicus can be obtained by a very simple and ingenious method, and the sperm slide specimen is prepared; the obtaining of the spermatophore can provide a raw material for the preparation and research of the sperm slide specimen of the Neoseiulus californicus, and the preparation of the sperm slide specimen can provide reference for understanding the sperm morphology of the Neoseiulus californicus and revealing the reproduction principle of the Neoseiulus californicus.

Description

technical field [0001] The invention relates to a method for making a sperm slide specimen of Neoseius californica, and belongs to the field of basic biological research of Neoseius california. Background technique [0002] Neoseius californica is a dioecious animal. It must undergo mating and fertilization between male and female mites before laying eggs and breeding offspring. Both male and female offspring develop from fertilized eggs. [0003] The existing information on the mating of male and female mite of Neoseius californica to form fertilized eggs is that the sperm of the male mite is stored in the sperm bag before entering the body of the female mite, and the female mite stuffs the sperm bag into the mating hole of the female mite (Reference: Liang Lairong , 1979, Plant Protection, How Mites Live), the sperm is slowly released from the seminal vesicle, transferred to the seminal vesicle and combined with the egg to form a fertilized egg. However, there are no repo...

AI Technical Summary

Problems solved by technology

[0004] However, because the individuals of Neoseius california are very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. During the mating process, the male and female mites are very closely stacked together, and the mating time is very short, only about 1 hour. , and the size of the spermatozoa is only a dozen microns and is hidden between the ventral surfaces of the male and female mites, which are in close contact with each other. It is difficult to see intuitively, and there is no way to obtain the spermatozoa in the existing data

Therefore, it is very difficult to obtain spermatozoa, and it is even more difficult to make spermatozoa slide specimens. It is necessary to develop a method to obtain the spermatozoa of Neoseius californica and to make slide specimens of Neoseiusius californica sperm