1613 results about "Sperm" patented technology

An intravaginal fertilization and culture container includes a main chamber and a microchamber. A culture medium, one or more oocytes and sperm are introduced through an access opening having an associated resealable closure device. The container is enveloped in a capsule of soft elastic material prior to lodgment in the posterior fornix. A loop on one capsule part adapts the length to anatomic variations. The microchamber has a channel which receives the catheter tip to facilitate embryo retrieval while eliminating risk of injury thereto. The microchamber permits embryos to be microscopically inspected in situ before transfer to the uterine cavity.

PCT No. PCT / JP96 / 02503 Sec. 371 Date Mar. 2, 1998 Sec. 102(e) Date Mar. 2, 1998 PCT Filed Sep. 4, 1996 PCT Pub. No. WO97 / 08946 PCT Pub. Date Mar. 13, 1997The present invention aims to provide a medium composition for in vitro fertilization, in particular, a composition usable in the culture of ova or early embryos which are fertilized eggs, the preparation or culture of sperm, and the pre-treatment of ova or sperm. The composition comprises, as its essential components, L-phenylalanine, L-tryptophan, L-lysine, L-threonine, L-valine, L-methionine, L-isoleucine, L-leucine, L-proline, glycine, L-alanine, L-tyrosine, L-histidine, L-arginine, L-taurine, L-aspartic acid, L-serine, L-asparagine, L-glutamic acid, L-glutamine and L-cystine, provided that at least a part of the L-cystine may be replaced by L-cysteine.

The present invention aims at extracting, separating and purifying the active components of Maka as one natural plant to obtain extractive with several medicinal and health care values. Fresh or dried Maka root material is extracted and separated to obtain active components and extracted residue. The active components includes glucosinolate and its decomposed product isothiocynate in 10-90 wt%,Maka amide and Maka olefin 5-70 wt% and sterol 2-30 wt%. The active components may be further prepared into capsule, tablet, and delayed releasing forms, and the prepared medicine and health food have the functions of treating climacteric syndrome, strengthening male's and female's sexuality, improving sexual function, etc.

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comrprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).

Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformly stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.

The invention discloses a method for acquiring an aromatic rice strain by targeting a Badh2 gene via CRISPR / Cas9 gene editing technology. The method comprises the following steps: separately targeting sequences, recognizable by Cas9, of every exon and intron of an aromatic gene by using the CRISPR / Cas9 gene editing technology and then cutting a genomic DNA sequence to initiate DNA restoration so as to obtain an afunctional Badh2 gene; with the callus of Indica rice, japonica rice or glutinous rice as a receptor material of genetic transformation and an mature embryo, young ear, ovary or the like as explant, carrying out induction so as to obtain a diploid callus, introducing a targeting vector into cells of the callus by using an Agrobacterium mediated transformation method, screening and identifying positive plants and separating the plants from a T1 colony so as to obtain an aromatic rice strain; and with anther, pollen, unfertilized ovary or the like as explant, carrying out induction so as to obtain a haploid callus, introducing the targeting vector into cells of the callus by using the Agrobacterium mediated transformation method, screening a positive callus, reduplicating the positive callus by using colchicine, carrying out differentiation to form seedlings and identifying genetic transformation positive plants so as to eventually obtain the aromatic rice strain.

There is provided a method for controlling a flow of sperms in a uterine tube formed by a uterine tube wall of a patient. The method comprises gently constricting (i.e., without substantially hampering the blood circulation in the uterine tube wall) at least one portion of the uterine tube wall to influence the flow of sperms in the uterine tube, and stimulating the constricted wall portion to cause contraction of the uterine tube wall portion to further influence the flow of sperms in the uterine tube. The method can be used for restricting or stopping the flow of sperms in the uterine tube, or for actively moving the fluid in the uterine tube, with a low risk of injuring the uterine tube.

A male undergarment providing a comfort barrier between the groin and outerwear with enhancements for promoting male fertility. By use of less restrictive (and confining) clothing adjuncts to the conventional undergarment, an environment for genitalia, primarily the testes, is acquired that will reduce the level of body heat accumulating in the zone or regions proximate those organs. Elements are used that will reduce the normally existing temperatures: a dam, consisting minimally of a fabric partition, for compartmentally segregating genitalia from the loins; thermally isolation, by use of suitable insulative / reflective fabrics, on the dam and between the segregated organs and groin regions; and, a vented, independent, scrotal compartment. Contrary to the modern norm, the genitalia are allowed to assume a more natural, pendulous suspension, one more conducive to the vitality of spermatozoa.

The invention relates to a method for the production of arachidonic acid in transgenic organisms, especially in transgenic plants and yeasts. The invention also relates to DNA sequences coding for a protein with enzymatic activity of a Δ5-desaturase from Phytophthera megasperma. The invention further relates to transgenic plants and plant cell and transgenic yeasts containing a nucleic acid molecule comprising a DNA sequence according to the present invention and having, on the basis thereof, an enhanced arachidonic acid synthesis in comparison with wild-type cells. The invention also relates to harvest products and propagating material of transgenic plants.

This invention provides FSH analogues having increased serum half-life relative to FSH. This invention also provides related compositions and methods for increasing fertility, egg production and spermatogenesis in a subject.

The present invention proposes formative agent of protein complex, in which a polyphenol is useful component, and the agent is useful as gene complex, cell adhesion inhibitor or immune tolerogen. The polyphenol of forming the agent is selected from catechin group consisting of epigallocatechin-gallate, tannic acids, or proanto-dianisidine, a protein of the protein complex is selected from proteins consisting of animal proteins composed of polypeptide chain of peptide-combined amino acids, vegetative proteins, nucleus proteins, glycogen proteins, lipo-proteins and metal proteins, the gene complex comprises by compositing genes by polyphenol catechins in order to introduce genes to cells of animals or human bodies, a cell composed of the cell adhesion inhibitor is selected from cells consisting of an animal cell including a stem cell, skin cell, mucosa cell, hepatocyte, islet cell, neural cell, cartilage cell, endothelial cell, epidermal cell, osteocyte or muscle cell isolated from human or animal organism, or sperm, ovum or fertilized egg of domestic animals or fishes and a tissue or an organ for transplantation of the immune tolerogen is selected from the tissue or the organ consisting of skin, blood vessel, cornea, kidney, heart, liver, umbilical cord, bowels, nerve, lung, placenta or pancreas.

This invention provides FSH analogues having increased serum half-life relative to FSH. This invention also provides related compositions and methods for increasing fertility, egg production and spermatogenesis in a subject.

A cattle and sheep non-animal-derived frozen semen diluent comprises at least one or the combination of the following ingredients: trehalose, propylene glycol, soybean lecithin, soluble soybean protein, nitrogen-trishydroxymethyl aminoethanesulfonic acid and dodecanoyl sodium sulfate. A production method of a cattle and sheet frozen semen by using the cattle and sheep non-animal-derived frozen semen diluent fully mixes the cattle or sheet semen and the dilutent, lowers the temperature and carries out the preservation. Compared with the prior art, the animal semen dilutent of the invention eliminates the animal-derived ingredients; the preservation method is more practical, the technical effects are better, thus thoroughly eliminating the potential risks of spreading various epidemic diseases between the animals and the people by the animal-derived ingredients in the dilutent. The cattle and sheep non-animal-derived frozen semen diluent can promote the popularization of animal artificial insemination technology to the utmost extent and improve the labor productivity and the economic benefits, thus having great economic and social values.

The invention discloses a simple, convenient and efficient fixed-point gene editing method relating to the fields of transgenic animal preparation, gene functional study and biomedicines. A testis injection method is a sperm-mediated gene transferring method developed by utilizing the sperm capacity of actively combining, transferring and integrating an exogenous DNA. By using the method disclosed by the invention, an animal testis injection method is optimized, a carrier constructed by utilizing a clustered regularly interspaced short palindromic repeat system (CRISPR / Cas9) is integrated with a sperm chromosome through multi-point testis injection or seminiferous tubule microinjection, so that the aims of deleting, replacing and inserting genes are achieved. Then, a transgenic animal descendant is obtained through various approaches such as natural mating and artificial insemination. According to the method, the advantages of a CRISPR / Cas9 gene editing system are sufficiently utilized, and the existing transgenic animal system is combined, so that the complexity of in-vitro cell operation and requirement on expensive precise instruments / equipment are avoided while the gene modification animal obtaining efficiency is greatly increased.

The invention discloses an animal sex control method based on Rbmy gene editing and application. The method controls animal sex by using CRISPR / Cas9 system to specifically cut Rbmy gene on Y chromosome. Rbmy gene encodes a germ cell specific nucleoprotein, this protein is an important factor related to spermatogenesis, and by cutting Rbmy gene, it is possible to deactivate Rbmy gene, thus Y sperm or fertilized ovum with Y sperm is deactivated and cannot develop into a normal embryo, birth ratio of female animals is finally increased and sex control is achieved. Therefore, sex ratio of animal offspring can be controlled through the method so that the ratio of female animals with better economic value is increased and production efficiency and economic effect are greatly improved.

The invention belongs to the health-care drink field, especially relating to a health-care heat-removing tea, containing the following components in terms of parts by weight: 9-15 parts of fructus momordicates, 3-6 parts of sterculia seed, 3-10 parts of Chrysanthemum morifolium, 3-15 parts of honeysuckles, 3-9 parts of balloonflower root, 6-15 parts of dwarf lilyturf, 6-15 parts of medlars, 6-30 parts of walnut meat, 3-9 parts of powdered liquorice, 6-9 parts of jujube and 3-6 parts of rock sugar. The health-care heat-removing tea has heat-removing and toxication-removing, liver-nourishing and visual acuity-improving, spittle-generating and dryness-wetting, lung-clearing and phlegm-eliminating, voice-raising and sore throat-relieving, kidney-nourishing and sperm-benefiting, blood-nourishing and verves-calming functions.

The invention relates to a method for reducing CRISPR / Cas9 mediated embryo gene editing missing rate. The method comprises the following steps: compounding a sgRNA sequence of a targeting gene and constructing a sgRNA expression plasmid carrying T7 promoter; in vitro transcribing Cas9mRNA and sgRNA; and importing intracytoplasmic sperm into unfertilized MII ovum by adopting intracytoplasmic sperminjection (ICSI) and meanwhile importing Cas9mRNA and sgRNA. According to the method provided by the invention, the targeting editing efficiency is high and the missing mutation rate is low.

This invention provides VEGF-FSH compounds having increased serum half-lives relative to either native VEGF or FSH, in which both VEGF and FSH are biologically active. This invention also provides related compositions and methods for increasing fertility, egg production and spermatogenesis in a subject, as well as methods for increasing vascularization in a tissue, particularly in ovarian tissue.

The present invention provides a method for enhancing embryo viability comprising administering at least one inhibitor of p53 or a p53-associated pathway to one or more of the following: the embryo, oocytes, sperm, a femme animal or a male animal.

The invention relates to a technical method of triploid seed production of rainbow trout. The technical method comprises the following steps: in proper time after sperm-egg binding, carrying out thermal (shock) treatment on oosperm in low-temperature environment in a certain time interval, then restoring the original low-temperature environment and carrying out cooling water incubation; leading more than 80% of oosperms which need to be incubated into diploid oosperm to generate triploid oosperm after thermal shock treatment; and raising the triploid oosperm into triploid offspring seed (larval fish). The implementation of the technical proposal stops the discharging of a secondary polar body of the oosperm, induces the oosperm to produce another type of oosperm which is fused by the egg, the sperm nucleus and the secondary polar body with respective monoploid chromosome and contains triploid chromosome, namely triploid oosperm, and realize industrialization of triploid seed production. Each thermal inducing incubator can produce more than 40,000 of triploid oosperms for one time, and each reproduction period (October to February) can produce more than 2,000,000 oosperms. The inductivity of the triploid is more than 80%.

A sperm collecting apparatus is provided and includes a container including a cylindrical container main unit that has an opening portion on at least one end face in a longitudinal direction thereof and a cap that is attached to and detached from the opening portion of the container main unit to close and open the opening portion, and a core member which is accommodated in said container and has an insertion room extending from an insertion port at one end face in a longitudinal direction therein. The core member includes a core main unit having said insertion port and the insertion room and a plurality of ribs provided upright from an outer face of the core main unit to cross one another, and sealed rooms are formed through the outer face of the core main unit.

An improved two-layered condom device which offers improved sensation to the male wearer compared to a conventional condom, which should result in increased compliance with usage. The device provides a dual barrier, with lubricant between an inner condom and an outer condom which may contain a chemical or chemicals to neutralize bacteria, viruses, and sperm, thereby making it superior to a conventional single-layered condom in preventing unwanted pregnancy and the spread of sexually transmitted diseases.

The invention relates to a traditional Chinese medicine composite in the field of Chinese herba preparations which nourish kidney yang. The technical scheme is that the composite consists of raw materials with the parts by weight as follows: 20 to 30 parts of ginseng, 10 to 20 parts of cinnamon, 20 to 30 parts of morinda offcinalis, 15 to 20 parts of dodder seed, 20 to 30 parts of human placenta, 10 to 20 parts of Chinese caterpillar fungus, 10 to 20 parts of cornu cervi pantotrichum, 20 to 30 parts of hippocampi, 20 to 30 parts of desertliving cistanche, 25 to 30 parts of rehmanniae vaporata, 10 to 20 parts of medicinal cyathula root, 20 to 30 parts of songaria cynomorium herb, 20 to 30 parts of short horned epimedium, 20 to 30 parts of astragalus hoangtchy, 15 to 20 parts of angelica, 20 to 30 parts of cortex eucommiae, 15 to 20 parts of licorice, 10 to 15 parts of radices saussureae, 20 to 25 parts of fructus lycii and 20 to 30 parts of cornus officinalis. The compatibility of the invention can tonifying qi and lifting yang, warm the interior and disperse the coldness, tonify the kidney and strengthen yang, encourage sperm and tonify marrow, promote blood circulation and remove blood stasis, and dispersing stagnated hepatoqi. The composite has very good curing and nourishing effects on impotence, spermatorrhea and frequent micturition which are caused by impotence due to deficiency of the kidney, softening of bones and muscles, insufficiency of kidney-yang and loss of essence and blood and can enhance the autoimmunity.

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.

A method of formulating high ambient temperature (room temperature and above) stable biologics (biologically active macromolecules, enzymes, serums, vaccines, viruses, pesticides, drug delivery systems, liposomes, cells suspensions, sperm, erythrocytes, other blood cells, stem cells, multicellular tissues, skin, heart valves) including secondary drying comprising at least two steps of stability drying at elevated temperature: 35° C., 40° C., 45° C., 50° C., and higher temperatures. The method could be applied to stabilize biologics encapsulated in alginate gel microspheres for better oral delivery. The method encompasses the following: microspheres are formulated using a cryo-encapsulation procedure comprised of mixing drops of frozen preservation mixture (To form the preservation mixture, biologics are mixed with preservation solutions containing sodium alginate.) with frozen drops of a calcium solution (i.e. calcium gluconate) and subsequent warming to form the gel particles.

A fish allogenous frozen-sperm induced gynogenesis method comprises a frozen storage method of the allogenous fish sperm, a genetic inactivation method, an induced method for the gynogenesis diploid fry and an identification method for the gynogenesis fry. The present invention establishes the method adopting the frozen-sperm of the sea bass to induce the egg gynogenesis of a plurality of sea fishes for the first time, which selects the cold-shock starting time, cold-shock temperature and the cold-shock durance time that are separately suitable for fish eggs of the strip-spotted flounder, the turbot and the canine-tooth paralichthys olivaceus to establish a micro-satellite marking identification method of the gynogenesis fry. The percentage rate adopting the present invention to acquire the gynogenesis fry of the strip-spotted flounder, the turbot and the canine-tooth paralichthys olivaceus is 7.8 percentages to 40.7 percentages, 26 percentages to 34 percentages and 10 percentages to 25 percentages respectively. The established method of the present invention is characterized in advancing, high efficiency, accuracy and reliability, which has an important application value in fish gender controlling and the production of the all-female fries and has a broad popularization and application prospect on fish cultivation and fish breeding.

The invention relates to external oculentum for treating amblyopia, astigmatism, strabismus, myopia and visual fatigue of teenagers and a preparation method thereof. The oculentum comprises the following components: 20 to 40 parts of feather cockscomb seed, 20 to 40 parts of flower of small pipewort, 20 to 50 parts of raspberry, 30 to 60 parts of cassia seed, 25 to 50 parts of medlar, 30 to 60 parts of pale butterflybush flower, 20 to 40 parts of saffron, 20 to 40 parts of wild chrysanthemum, 30 to 50 parts of danshen root, 30 to 50 parts of largetrifoliolious bugbane rhizome, 20 to 40 parts of mint, 20 to 40 parts of storax, 30 to 50 parts of calamine, 2,000 to 3,000 parts of Vaseline, 300 to 500 parts of liquid paraffin and appropriate amount of water. The external oculentum is prepared by removing impurities from the medicinal raw materials; decocting, filtering and concentrating the medicinal raw materials; and adding the Vaseline and the liquid paraffin. The external oculentum is scientifically prepared according to compatible principle of monarch, minister, assistant and guide of Chinese medicinal theory. The external oculentum can effectively treat the amblyopia, the astigmatism, the strabismus, the myopia, the visual fatigue and ametropia of the teenagers, and has the advantages of exact curative effect and no toxic or side effects on human bodies.

The invention discloses a shallow sea oyster single body cultivation method. The method comprises the following steps of sperm and egg obtaining, fertilization, incubation, baby oyster cultivation, young oyster collection, middle cultivation of young oyster seeds in a sea area, separation of the young oysters from matrixes, and placing of oyster single bodies in baskets and cultivation. According to current oyster single body cultivation, the young oyster single bodies cultivated in a room are adopted and directly placed in the sea area for cultivation, as the young oysters and cultivation cage screens are small, a cultivation cage is blocked by float sludge in the sea area easily, the young oysters are mutually piled up easily and grow slowly, and the survival rate is low. The method solves the problems that the existing oyster single body cultivation effect is poor, the oysters grow slowly, and the survival rate is low. The method is easy and convenient to carry out, and high in operability and survival rate, and the oyster single bodies grow fast.

The present invention relates to compounds capable of acting as androgen receptor, antagonists, pharmaceutical formulations containing the same, and methods of use thereof. Such uses include, but are not limited to, use as antitumor agents, particularly for the treatment of cancers such as colon, skin and prostate cancer and to induce androgen receptor antagonist activity in a subject afflicted with an androgen-related affliction. Examples of androgen-related afflictions include, but are not limited to, baldness, hirsutism, behavioral disorders, acne, and uninhibited spermatogenesis wherein inhibition of spermatogenesis is so desired.

The invention relates to wine preserving health and keeping young. The production method and characteristic mixture ratio of the invention are as follows: medlar, shizandra berry sesame, milk veteh, processed fuzi, walnut meat, prepared polygalae, achyranthes, prepared fleece flower root, cibotium root, dodder, leek seed, epimedium powder, root of rehmannia, cinnamon, cynamorlum herba cistanches, fructus cnidii selinum, tuckahoe, cedar seed, raspberry, ligustrum lacidum aiton, caulis cynomorii, prepared rhizome of rehmannia, angelica, fructus psoraleae, 230 to 260g of honey, and 300 to 9000g of high-quality daqu wine of 55 degrees added to be heated to boil and form the invention after cooling and filtering, followed by bottling. The wine has the effects of invigorating vital energy, hematogenesis, invigorating blood circulation, tranquilizing by nourishing the heart, benefiting kidney, yin and yang, strengthening muscle and bone, through channels, warming the kidney and strengthening yang, benefiting sperm and supplementing marrow, protecting liver and strengthening spleen, attempering five Zang-organs and six Fu-organs, growing and blacking hair, maintaining beauty and revivifying, flushing the spirit, eliminating fatigue, promoting metabolism, activating senile cell, building up immune function of human, delaying senescence and keeping youth.