The invention discloses a method for efficiently preparing high-quality cloned embryos, belonging to the field of
embryo transplanting methods. The method provided by the invention comprises the following steps of: agitating and screening to obtain sheep oocytes in the same
cell period and
transplanting a supplier
cell into the sheep oocytes through utilizing
microscopy injection; then treating by utilizing a fusion solution and putting the treated
embryo into a mature solution to be cultured to form a fused
embryo; and chemically activating and then starting and reconstructing embryo cleavage. According to the method provided by the invention, the sheep oocytes which are in the same
cell period and have the uniform mature time through an agitating method are acquired, a new method for picking the good sheep oocytes is provided and the mature rate of the sheep oocytes is improved. According to the method provided by the invention, the operation efficiency of
nuclear transfer is improved by utilizing a method of simultaneously denucleating through
extrusion and carrying out
nucleus injection by utilizing the same injection needle. The fusion efficiency is improved to more than 95% by using the fusion solution which is developed by the method provided by the invention, the successful rate of the cloned embryo is greatly improved and the subsequent embryo development is not influenced. The method provided by the invention has the advantages of reducing the loss of the embryos in the process of preparing the cloned embryo and improving the efficiency of preparing the sheep cloned embryos; and the method provided by the invention is a method for efficiently preparing the sheep cloned embryos with good quality and has a wide application prospect.