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240 results about "Ploidy" patented technology
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Ploidy (/ˈplɔɪdi/) is the number of complete sets of chromosomes in a cell, and hence the number of possible alleles for autosomal and pseudoautosomal genes. Somatic cells, tissues, and individual organisms can be described according to the number of sets of chromosomes present (the "ploidy level"): monoploid (1 set), diploid (2 sets), triploid (3 sets), tetraploid (4 sets), pentaploid (5 sets), hexaploid (6 sets), heptaploid or septaploid (7 sets), etc. The generic term polyploid is often used to describe cells with three or more chromosome sets.
Methods and compositions for determining ploidy
The invention provides improved methods, compositions, and kits for detecting ploidy of chromosome regions, e.g. for detecting cancer or a chromosomal abnormality in a gestating fetus. The methods can utilize a set of more than 200 SNPs that are found within haploblocks and can include analyzing a series of target chromosomal regions related to cancer or a chromosomal abnormality in a gestating fetus. Finally the method may use knowledge about chromosome crossover locations or a best fit algorithm for the analysis. The compositions may comprise more than 200 primers located within haplotype blocks known to show CNV.
Owner:NATERA
Methods for non-invasive prenatal ploidy calling
PendingUS20190211392A1Fill in the blanksMathematical modelsMicrobiological testing/measurementMedicinePloidy
The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.
Owner:NATERA
Methods and systems for calling ploidy states using a neural network
PendingUS20210327538A1Accurate callInsight into the health of the embryoMicrobiological testing/measurementProteomicsTest samplePloidy
A method of calling a ploidy state using a neural network includes determining, for a training sample, genetic sequencing data or genetic array data for a plurality of genetic positions, determining respective true ploidy state values for a plurality of genetic segments, each genetic segment respectively comprising at least some of the plurality of genetic positions, based on the genetic sequencing data or genetic array data, and determining a neural network comprising one or more layers for calling respective ploidy state values, the neural network defined at least in part by a plurality of weights. The method further includes iteratively modifying the weights using specific processes. The method further includes calling, for a test sample, a ploidy state for a target genetic region by propagating genetic sequencing data for the test sample or genetic array data for the test sample through the modified neural network.
Owner:NATERA
Culture method of high diplont rate sporule regeneration plant of broccoli
ActiveCN101617630AImprove embryo emergence rateImprove germination rateCultivating equipmentsPlant tissue cultureSporeEmbryo
The invention discloses a culture method of high diplont rate sporule regeneration plant of broccoli, which belongs to the technical field of plant tissue culture and comprises the following steps: (1), preparing a culture medium; (2), culturing the high diplont rate sporule regeneration plant of broccoli: 1), selecting a donor plant and a bud, 2), sterilizing the bud, 3), pre-treating and culturing the bud, 4), separating, mixing and subpackaging of bud sporule, 5), culturing sporule embryoid, 6), differentiating and culturing a regeneration plant, 7), rooting and transplanting the regeneration plant and 8), detecting ploidy of the regeneration plant. The invention obviously increases the germ extraction rate and the rate of emergence of the sporule culture of the broccoli and the diplont rate of the regeneration plant respectively to 120 embryos / bud, 70 percent and more than 70 percent from common 80 embryos / bud, 50 percent and about 50 percent, thereby improving the breeding efficiency of the broccoli. The method can be popularized and applied to a vegetable breeding department or company.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Method for establishment of hybrid strain between bluntsnout bream and topmouth culter and breeding method of topmouth bream
ActiveCN102972319AGenetic stabilityFast growthClimate change adaptationPisciculture and aquariaPloidyGenetic stability
The invention discloses a method for establishment of a hybrid strain between bluntsnout bream and topmouth culter. The bluntsnout bream is used as a female parent, the topmouth culter is used as a male parent, the hybrid strain F1 of the bluntsnout bream and the topmouth culter is obtained through distant hybridization and is then bred and tested to obtain a bisexual fertile F1; then F1 is cultured as an object, selfing of the bisexual fertile F1 is performed, and a diploid F2 of the bluntsnout bream and the topmouth culter is obtained through incubation and breeding; and the ploidy and sterility of F2 are tested to obtain a bisexual fertile F2, and thus the heterologous bream and culter stain with genetic stability and bisexual sterility is obtained. The invention also discloses a breeding method of topmouth bream. The bisexual fertile diploid hybrid strain F1 of the bluntsnout bream and the topmouth culter and the bisexual fertile F2 of the bluntsnout bream and the topmouth culter are obtained by the establishment method, and backcross breeding is performed with the bluntsnout bream to obtain the hybrid topmouth bream. By adopting the breeding method, the novel hybrid topmouth bream with characteristics of high growth speed, high resistance, high meat quality, attractive shape and the like can be prepared.
Owner:湖南岳麓山水产育种科技有限公司
Systems and Methods for Determining Copy Number Variation
A method of identifying a copy number variations reads includes mapping reads to a reference genome, computing coverage for a plurality of tiles, and normalizing the coverage for a tile based on a coverage mode across the plurality of tiles. The method further includes determining a score for the plurality of tiles being in a plurality of ploidy states, determining a maximum score path across the tiles and through the ploidy states, and providing a copy number determination based on the maximum score path.
Owner:LIFE TECH CORP
Culture method of high diplont rate sporule regeneration plant of broccoli
InactiveCN101617631AImprove embryo emergence rateImprove germination rateCultivating equipmentsHorticulture methodsSporePloidy
The invention discloses a culture method of high diplont rate sporule regeneration plant of broccoli, which belongs to the technical field of plant tissue culture and comprises the following steps: (1), preparing a culture medium; (2), culturing the high diplont rate sporule regeneration plant of broccoli: 1), selecting a donor plant and a bud, 2), sterilizing the bud, 3), pre-treating and culturing the bud, 4), separating, mixing and subpackaging of bud sporule, 5), culturing sporule embryoid, 6), differentiating and germinating and culturing a regeneration plant, 7), rooting and transplanting the regeneration plant and 8), detecting ploidy of the regeneration plant. The invention obviously increases the germ extraction rate and the rate of emergence of the sporule culture of the broccoli and the diplont rate of the regeneration plant respectively to 80 embryos / bud, 70 percent and more than 70 percent from common 60 embryos / bud, 50 percent and about 50 percent, thereby improving the breeding efficiency of the broccoli. The method can be popularized and applied to a vegetable breeding department or company.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Breeding technique of obtaining triploid grape and ploidy early identification using embryo
InactiveCN101283669ASpeed up the breeding processImprove breeding efficiencyDead plant preservationMicrobiological testing/measurementVitis viniferaAgricultural science
The invention discloses a culture method for triploid grape culture by embryo rescue and early identification of ploidy. The invention aims to cultivate triploid grape by embryo rescue and overcome the problem of seed abortion of hybrid embryo between diploid and tetraploid grape cultivars in conventional breeding. After a hybrid seed is obtained, chromosome count is performed by the combination of flow cytometry and wall degradation hypotonic method to identify the ploidy of the hybrid progeny. The invention increases the breeding efficiency of triploid grape, accelerates the breed selection of triploid grape variety and provides a new approach to the culture of seedless grape variety.
Owner:NORTHWEST A & F UNIV
Method for cultivating triploid of red sage root
InactiveCN101124887AReduce nutrient consumptionHigh biological yieldMicrobiological testing/measurementPlant genotype modificationPloidyAsexual reproduction
The invention relates to a cultivation method of the triploid salvia, the steps of the invention are that: not completely ripe good salvia seeds are collected to put into the 1 / 2MS culture medium with colchicine for mutagenesis cultivation; then the seeds are transferred into a normal 1 / 2MS culture medium for cultivation, the root tip of the seedling is fetched to perform the ploidy identification of the chromosome after the preceding process of the chromosome, and the seedling with a chromosome quantity of 2n=4x=32 is the tetraploid salvia; the tetraploid salvia is taken as a female parent and the diploid salvia is taken as a male parent to perform the crossbreed and the obtaining seed is a triploid salvia plant. The nutrient organs such as the root, stem and leaf and so on of the triploid salvia plant obviously become bigger and present remarkable crossbreed advantages, and the root medicinal material output of the triploid salvia is increased by over 30 percent. Moreover, the triploid salvia has flowers, but no seeds, thus decreasing the nutrition consumption and the crossbreed advantages of the triploid salvia can be kept over 10 years through the asexual reproduction, which enhances the disease resistance and stress resistance with strong applicability, thus being applicable to planting in every part of the country. Besides, the crossbreed advantages produced by the novel variety triploid production are able to improve the output of the salvia medicinal material planting in a large scale.
Owner:NANKAI UNIV
Method for doubly improving marigold genotype by utilizing chromosomes
InactiveCN101766119AEasy to control humidityEasy to control temperatureSeed and root treatmentMicrobiological testing/measurementBiotechnologyPloidy
The invention belongs to the technical field of flower breeding, in particular to a method for doubly improving a marigold genotype by utilizing chromosomes, which is characterized in that male sterile dual-purpose line of a seed or a stem tip of marigold is treated by adopting a colchicine solution so as to enable the number of the chromosomes of the marigold to be doubled, thereby providing a breeding starting material of the improvement of the marigold genotype. The method comprises the following steps of: bud forcing treatment, colchicine doubling treatment, rinsing relief treatment, cultivation management, chromosome ploidy authentication and the like. The preferable treatment is described as follows: applying colchicine solution with concentration of 0.05 percent to marigold germinating seeds and soaking for 3 to 6 hours, and thus, the survival rate of plants is high, and the doubling efficiency also reaches 88.89 percent. The invention can effectively control the environment temperature and humidity when the seeds germinate; the repeatability of a test is high, the operation is convenient and time and labor are saved. The invention is suitable for the improvement of the marigold genotype.
Owner:HUAZHONG AGRI UNIV
Methods for non-invasive prenatal ploidy calling
Disclosed herein are methods for determining the copy number of a chromosome in a fetus in the context of non-invasive prenatal diagnosis. In an embodiment, the measured genetic data from a sample of genetic material that contains both fetal DNA and maternal DNA is analyzed, along with the genetic data from the biological parents of the fetus, and the copy number of the chromosome of interest is determined. In an embodiment, the maternal serum is measured using a single-nucleotide polymorphism (SNP) microarray, along with parental genomic data, and the determination of the chromosome copy number is used to make clinical decisions pertaining to the fetus.
Owner:NATERA
High yield maize derivatives
InactiveUS20090162477A1High in sugarImprove digestibilityDough treatmentBiofuelsHybrid speciesPloidy
A Method for producing triploid corn seeds and plants is described. The method comprises combining two parent inbreds of different ploidy levels, wherein one parent inbred is a tetraploid (4N) and the other parent is a diploid (2N) so as to produce a triploid hybrid corn seed; and cultivating the triploid hybrid corn seed to form a triploid corn plant. Usage of triploid corn plants as an economic source of sugar and ethanol is described, as is the production of molasses, rum and fodder from plant material of the low sterility triploid corn plants.
Owner:BIOFUELS GENETICS
Method for analysis of cellular DNA content
ActiveCN102971617AMicrobiological testing/measurementPreparing sample for investigationAPOPTOSIS/NECROSISNucleotide
Owner:CHEMOMETEC AS
Method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala
ActiveCN104585083ABeautiful bodyFast growthClimate change adaptationPisciculture and aquariaBroodstockCyprinus
A method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala includes the following steps that firstly, female cyprinus carpio and male megalobrama amblycephala which have obvious sexual maturity characteristics and good somatic features are selected as parent fishes for special pool breeding, then, artificial spawning induction is conducted on the parent finishes in a breeding season, the parent fishes with a good spawning induction effect are selected to be subjected to artificial dry-method fertilization, running water incubation is conducted on fertilized embryos in a pure water incubation device, fish fry are raised after being incubated, the raised fish fry are detected and screened, hybrid offspring of the cyprinus carpio and the megalobrama amblycephala are obtained through flow cytometry DNA content determination and renal tissue chromosome ploidy detection, and the hybrid offspring comprise diploid natural gynogenesis cyprinus carpio, diploid natural gynogenesis mirror carps, diploid fish like the cyprinus carpio and tetraploid hybrid fish. The method has the advantages that the rate of fertilization is high, the incubating rate is high, the offspring are excellent in character, and the method has great significance in genetic breeding.
Owner:湖南岳麓山水产育种科技有限公司
Methods and systems and analysis of CGH data
Methods, systems and computer readable media for analysis of comparative genomic hybridization data analysis, including creating a centralization curve from log ratio data values for DNA copy numbers of a genome of a test sample relative to a genome of a reference sample, wherein the reference sample has a known ploidy, and the test sample has a same copy number as the reference sample in normal, non-aberrant genomic regions; identifying a peak corresponding to regions of normal copy number in the centralization curve; centralizing the log ratio data so that the peak corresponding to regions of normal copy number is centered at a log ratio value of zero; calculating a mathematical measurement that is a function of the width of the peak corresponding to regions of normal copy number; calculating a tolerance value as a function of the mathematical measurement; and outputting the tolerance value. Methods, systems and computer readable media are provided to create a centralization curve from log ratio data values for DNA copy numbers of a genome of a test sample relative to a genome of a reference sample, wherein the reference sample has a known ploidy, and the test sample has a same copy number as the reference sample in normal, non-aberrant genomic regions; identify peaks in the centralization curve; assign copy numbers to the identified peaks; plot expected ratios, based on the assigned copy numbers, of the peaks versus observed ratios of the peaks calculated from the log ratio data values; conclude that the assigned copy numbers are correct if the plot of the expected ratios versus the observed ratios is substantially linear; and output at least one of the plot of expected ratios versus observed ratios, and a conclusion as to whether the plot is substantially linear.
Owner:AGILENT TECH INC
Method for obtaining chrysanthemum distance hydrid using goung embryo rescue
InactiveCN1561694ABroaden the genetic baseDistant hybridizationPlant genotype modificationGermplasmInterspecific hybridization
A method for preparing the distant hybrid of chrysanthemum by embryo rescue includes hybridization between wild diplontic chrysanthemum and cultured hexaplontic 'Huang Ying', embryo rescue by ovary culture, discriminating their descendants by different approaches, and choosing the descendant with new characters.
Owner:NANJING AGRICULTURAL UNIVERSITY
Cultivation method for high yield and high quality industrial cannabis sativa
InactiveCN109287485AHigh induction rateHigh differentiation rateHorticulture methodsPlant tissue cultureFiberCannabis
The invention provides a cultivation method for high yield and high quality industrial cannabis sativa. The method mainly includes the steps of: 1. flower bud collection and screening; 2. flower bud sterilization and inoculation; 3. anther induction culture; 4. callus redifferentiation; 5. adventitious bud proliferation and rooting; 6. pollen plant ploidy detection, and rejection of non-diploid plants; 7. transplantation of pollen plant seedlings; and 8. cross breeding, thus obtaining a holandric new line. The invention for the first time establishes a complete system for industrial cannabis sativa anther culture, also utilizes the method to produce super-male plants and female plants, and the plants are subjected to cross breeding to cultivate the holandric new line, thus significantly improving the yield and quality of cannabis sativa fiber, and providing a material basis for the development and utilization of cannabis sativa and cannabis sativa fiber.
Owner:江苏高航农业科技有限公司
Breeding method of poplar haploid
ActiveCN102550405AGrow vigorouslyHigh reproductive coefficientPlant tissue cultureHorticulture methodsAgricultural sciencePloidy
The invention discloses a breeding method of poplar haploid and belongs to the field of selective breeding of poplar haploid. The breeding method comprises the following steps: (1) inoculating sterilized poplar anthers to a callus induction culture medium for callus induction culture to obtain poplar calli; (2) inoculating the poplar calli to an adventitious bud differentiation culture medium for differentiation culture of adventitious buds to obtain poplar adventitious buds; (3) inoculating the poplar adventitious buds to a rooting culture medium for rooting culture to obtain test-tube plantlets; and (4) performing ploidy identification to obtain poplar haploid plants. The breeding method of poplar haploid solves the problems occurring in poplar haploid breeding, has the advantages of high callus induction rate, high callus organ differentiation rate, high breeding rate and simple induced breeding operation, can produce high-quality poplar test-tube plantlets with high yield during a short time, achieves the purpose of large-scale industrial production, and provides a material basis for breeding, quality improvement and genetic research of poplar.
Owner:BEIJING FORESTRY UNIVERSITY
Industrial production method of Cymbidium germchit based on sexual hybridization
InactiveCN102301950AIncrease varietyProliferation line highCultivating equipmentsHorticulture methodsPollinationBottle
The invention relates to an industrial production method of Cymbidium germchit based on sexual hybridization. The method provided by the invention comprises the following steps of: firstly identifying chromosome number and ploidy of Cymbidium parents; selecting pairing hybridized combinations according to the ploidy; carrying out a sexual hybridization pollination of the Cymbidium; managing the pollinated Cymbidium; carrying out a nonsymbiotic germination culture on Cymbidium seeds; inducing and differentiating protocorms (PLBs) of the Cymbidium seeds; differentiating bigger seedlings of the Cymbidium; transplanting the Cymbidium seedlings into bottles; and managing the cultivation of the Cymbidium transplanted seedlings. On the basis of indentifying the chromosomes of the Cymbidium parents, the method provided by the invention selectively pairs the hybridized combinations and carries out the sexual hybridization pollination, so that the aims of improving varieties and breeding new varieties are achieved; then a lot of Cymbidium germchit can be industrially produced through a tissue culture method.
Owner:NANKAI UNIV
Reagent for detecting copy number of EGFR gene and ploidy of chromosome 7
ActiveCN101899504AHelps with sensitivityAids in prognostic judgmentMicrobiological testing/measurementFluorescence/phosphorescenceTyrosine-kinase inhibitorPloidy
The invention discloses a reagent for detecting a copy number of an EGFR gene and the ploidy of a chromosome 7. The reagent comprises a fluorescence probe RP11-339F13 used for detecting the copy number of the EGFR gene and a fluorescence probe RP11-144H20 used for detecting the ploidy of the chromosome 7, wherein the RP11-339F13 is cloned by BAC, which covers the whole EGFR gene, and marked with rhodamine fluorescein; and RP11-144H20 is cloned by specific BAC of the centromere of the chromosome 7 and marked with fluorescein isothiocyanate. The reagent can quickly, simply and conveniently detect the copy number of the EGFR gene and the ploidy of the chromosome 7, contributes to the judgment of medicament susceptibility and prognostic judgment, and has great significance for the guidance of use of molecular targeted medicament tyrosine kinase inhibitors (TKIs) of tumors, such as non-small cell lung cancer and the like.
Owner:合肥艾迪康医学检验实验室有限公司
Asparagus all-male breeding method
InactiveCN105941152AIncreased haploid doubling efficiencyHigh doubling efficiencyPlant tissue cultureHorticulture methodsDiseaseF1 generation
The invention provides an asparagus all-male breeding method. The breeding method comprises the following specific steps: (1) selecting and pretreating anther; (2) preparing an asparagus anther induction medium; (3) performing high sugar treatment on the anther, then disinfecting and inoculating; (4) performing induction culture on the anther; (5) performing callus differentiation culture; (6) enabling green buds to expand propagation, multiplication and root; (7) hardening seedlings and transplanting; (8) observing chromosome ploidy, removing non-diploid plants, and distinguishing super male plants and female plants; (9) screening and hybridizing asparagus super male plants and female plants. According to the all-male breeding method, asparagus anther culture plants are obtained through an efficient anther culture way, the diploid super male plants and female plants are obtained after the non-diploid plants are removed, further the asparagus super male plants and female plants with excellent agronomic characters are selected for hybridization, thus obtaining a high-yield and high-quality F1 generation all-male system high in growth rate and strong in disease resistance. The asparagus super male plants and female plants are high in inductivity and fewer in abnormal plant, and the obtained all-male system is excellent in quality and great in industry value.
Owner:田东县举家富现代农业开发有限公司
Noninvasive detection method for screening healthily grown blastulas
ActiveCN105861658AHigh precisionRelieve painMicrobiological testing/measurementEmbryo transferPloidy
Owner:GUANGZHOU NVWA LIFE TECH CO LTD
Novel germplasm creating method by distant hybridization of Brassica campestris ssp. chinensis Makino and Raphanus sativus
InactiveCN103947536AGenetic stabilityShorten breeding timePlant tissue cultureHorticulture methodsBrassicaGenetic diversity
The invention discloses a novel germplasm creating method by distant hybridization of Brassica campestris ssp. chinensis Makino and Raphanus sativus. The method uses autotetraploid Brassica campestris ssp. chinensis Makino as a female parent and autotetraploid Raphanus sativus as a male parent to conduct artificial sexual crossbreeding; and repeated pollination and in vitro embryo rescue techniques are employed to obtain distant hybridization novel germplasm of autotetraploid Brassica campestris ssp. chinensis Makino and autotetraploid Raphanus sativus. The invention for the first time adopts sexual crossbreeding of the autotetraploid Brassica campestris ssp. Chinensis Makino and autotetraploid Raphanus sativus and in vitro embryo rescue technique to obtain new germplasm of distant hybridization. Compared with traditional diploid hybridization, which obtains zygotic embryo for ploidy reduplication, the method does not require chromosome doubling operation, and can significantly shorten the breeding time; and the hybrid F1 can reach stable inheritance. The novel germplasm created by the invention creation has the genetic materials of Brassica campestris ssp. chinensis Makino and Raphanus sativus, and a wider genetic diversity.
Owner:NANJING AGRICULTURAL UNIVERSITY
Breeding method for kumquat triploid
The invention discloses a breeding method for kumquat triploid. The method comprises the following steps: tetraploid mutagenesis, sprouting cultivation of seeds after mutagenesis, chromosome ploidy inspection, cultivation of tetraploid plants, tetraploid fruit harvesting and seed extraction, tetraploid embryo separation and cultivation, embryo chromosome detection, and cultivation of triploid seedlings. According to the method, a sexual hybridization process is avoided; the interference of nucellar embryo is eliminated; the triploid is directly separated and cultivated from polyembryonic tetraploid seeds; and the method has the advantages of being simple to operate, and short in breeding period.
Owner:GUANGXI UNIV
Method for utilizing plant heterosis
The invention discloses a method for utilizing plant heterosis. The method comprises the following steps: S1, converting meiosis of germ cells of a hybrid into similar mitosis by using a gene mutationor genetic engineering technology so as to obtain gametes consistent with a genotype and chromosome ploidy of the hybrid; and S2, inducing the gametes to develop into seeds or plants. By applying thetechnical scheme of the invention, hybrid seeds can generate cloned seeds or plants which are completely consistent with its genotype and chromosome ploidy, so that the heterosis can be utilized fora long time, and the existing problems of difficulty in parent-to-parent hybridization, low seed production yield, high hybrid seed cost and the like caused by inconsistent flowering periods and the like in the utilization of the heterosis are solved.
Owner:CHINA NAT RICE RES INST
Detection method and equipment for measuring instability of genome, terminal equipment and computer readable storage medium
The invention provides a detection method for measuring the instability of genome. The detection method comprises the following steps: (1) acquiring sequencing data; (2) determining a BAF value and an LRR value; (3) removing outliers; (4) carrying out at least one round of segmentation treatment; (5) combining breakpoint sets; (6) carrying out merging processing on the second-stage sorting fragments; (7) carrying out filtering and cutting treatment on the three-stage separation fragments; (8) carrying out same-distribution inspection; (9) determining a BAF mean value and a copy number mean value; (10) performing planar coordinate axis clustering mapping on the BAF mean value and the copy number mean value percentage ordinal number of the four-stage separation segment so as to determine the tumor purity of the tumor sample in the to-be-detected sample; (11) determining a ploidy correction factor of the sample to be detected; (12) performing BAF numerical correction and copy number correction; (13) determining an A genotype parameter value and a B genotype parameter value; and (14) determining a variation type.
Owner:BGI GENOMICS CO LTD +2
Flaking method of petunia chromosome
InactiveCN104215485AHigh mitotic indexPrevent or disrupt formationPreparing sample for investigationAnimal scienceStaining
The invention discloses a flaking method of petunia chromosome. The method comprises the following steps: getting a petunia young bud at 8:00-10:00 in the morning of a clear day, placing in 0.002mol.L<-1> 8-hydroxyquinoline aqueous solution for pre-treating at low temperature, transferring into a Carnoy's fluid to be fixed, performing low-permeation through 0.075mol.L<-1> potassium chloride, and then water-bathing at 60 DEG.C in 1mol.L<-1> HCl solution for decomposing, washing and then low-permeating through the distilled water, and staining through aceto-carmine staining solution, transferring the young bud onto a glass slide, cutting to get a pistil stigma part, flaking and performing microscopy. The bud size, the pretreatment method, the decomposition and staining method and time and the flaking processes of petunias with different ploidy at optimal sampling time are determined, the obtained chromosome is uniform in dispersion, easy to count and easy for performing karyotype analysis, the operation is simple and convenient, the efficiency is high, and the method has a good application prospect.
Owner:JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
Culture method for brassica oleracea L. var. acephala microspore regeneration plant
InactiveCN102228003AImprove embryo emergence rateImprove utilization efficiencyHorticulture methodsPlant tissue cultureSporeHeat shock
Owner:ZHEJIANG UNIV +1
Cultivation method of gynogenesis megalobrama amblycephala
ActiveCN101720697AFacilitates genetic researchShorten breeding timeClimate change adaptationPisciculture and aquariaEmbryoPloidy
The invention discloses a cultivation method of a gynogenesis megalobrama amblycephala, which comprises the following steps of: firstly selecting and breeding a female megalobrama amblycephala and a male erythroculter ilishaeformis bleeker as parent fishes; then carrying out artificial induced spawning to obtain the sperms of the erythroculter ilishaeformis bleeker and the ripe ovums of the megalobrama amblycephala; mixing the erythroculter ilishaeformis bleeker sperms inactivated by ultraviolet rays with the ripe ovums of the megalobrama amblycephala; 3-4 minutes later, placing the ovums activated at 0-4 DEG C for cold shock treatment for 20-30 minutes; and after incubating embryos treated by cold shock at 23-24 DEG C, obtaining diploid gynogenesis megalobrama amblycephalas through a flow type cell DNA content determination method and a chromosome ploidy detection method of peripheral blood cell culturing. The cultivation method of the gynogenesis megalobrama amblycephala has the advantages of short cultivation time, fast growth speed, high incubation rate, excellent offspring property, and the like, and has great importance on aspects of fish inheritance breeding and biological evolution researches.
Owner:HUNAN NORMAL UNIVERSITY
Copy number variation detection method and device
The invention provides a copy number variation detection method and device. The detection method comprises the following steps: acquiring sequencing comparison data of a to-be-detected sample; calculating the sequencing depth of each base site in the sequencing comparison data; dividing a reference genome into a plurality of bins, and calculating the copy number of each bin of the to-be-detected sample by utilizing the sequencing depth of each basic group site; and combining bins of which the copy number is different from the ploidy of the specified contig to obtain an area in which the embryonic system copy number varies. In the aspect, deletion or repetition of a gene exon in the length more than 1000bp can be detected; compared with a chip method in the prior art, the method of the invention has higher coverage and resolution and more accurate copy number evaluation for detecting the CNV, not only can the copy number variation condition of some known sites be detected, but also theunknown copy number variation condition can be detected, and the detection sensitivity is improved.
Owner:北京橡鑫生物科技有限公司 +2
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